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Luong, Hoang Van,Long, Nguyen Van,Duong, Vu Binh,Toan, Nguyen Linh,Minh, Nguyen Van,Quang, Le Bach,Kim, Nam-Hyuck,Byun, Sang-Yo The Korean Society for Biotechnology and Bioengine 2009 KSBB Journal Vol.7 No.4
This study was initiated to investigate the impacts of media types and other components on the callogenensis and cell mass production of Panax vietnamensis in the first step of the cell biomass procedure. Four media were checked: Murashige-Skoog (MS), White, Gamborg and Nitch-AII. All the four media were shown potential media for Panax vietnamensis callogenensis and cell mass production, in which the MS medium showed the best results: the successful callogenensis ratio and cell mass formation were 30% and 62,93 ${\pm}$ 3,63 mg (DW) respectively, the Nitch medium showed the lowest results: the successful callogenensis ratio and cell mass formation were 15% and 27,10 ${\pm}$ 2,24 mg (DW) respectively. The results showed that the MS medium is the most suitable medium for Panax vietnamensis callogenensis and cell mass production.
Hoang Van Luong,Nguyen Van Long,Vu Binh Duong,Nguyen Linh Toan,Nguyen Van Minh,Le Bach Quang,Nam Hyuck Kim,Sang Yo Byun 한국생물공학회 2009 KSBB Journal Vol.24 No.2
This study was initiated to investigate the impacts of media types and other components on the callogenensis and cell mass production of Panax vietnamensis in the first step of the cell biomass procedure. Four media were checked: Murashige-Skoog (MS), White, Gamborg and Nitch-All. All the four media were shown potential media for Panax vietnamensis callogenensis and cell mass production, in which the MS medium showed the best results: the successful callogenensis ratio and cell mass formation were 30% and 62,93 ± 3,63 mg (DW) respectively, the Nitch medium showed the lowest results: the successful callogenensis ratio and cell mass formation were 15% and 27,10 ± 2,24 mg (DW) respectively. The results showed that the MS medium is the most suitable medium for Panax vietnamensis callogenensis and cell mass production.
Hoang, Van Minh,Kim, Bao Giang,Phan, Thi Hai,Trinh, Dinh Hoang,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Thuy Linh,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
Scientific evidence on all aspects of smoking amongst youth is very important for designing appropriate interventions to reduce smoking among this vulnerable population. This paper describes current access to anti-smoking information among school children aged 13 to 15 years in Vietnam in 2014 and examines its potential impact on preventing smoking initiation. The data used in this paper were obtained from the 2014 Global Youth Tobacco Survey (GYTS) in Vietnam. Students were asked questions about their level of awareness of anti-smoking information from various sources in the past 30 days and about lessons in school regarding the dangers of tobacco use during the last 12 months. Those who have never smoked were asked "whether or not they thought about avoiding cigarettes because of health warnings on cigarette packages" and answers were analyzed in combination with data on access to anti-smoking information from other sources. The prevalence of exposure to antismoking campaigns was high among school children in Viet Nam: 55.3% of current smokers reported thoughts of smoking cessation because of health warnings on cigarette packages; 60.5% of never smokers avoided initiating smoking because of the same health warnings. The potential impact of graphic health warnings to prevent school-aged children from smoking initiation would be stronger if there was concurrent access to anti-smoking programs on the dangers of tobacco use in schools. However, school education for tobacco prevention and control has not been as strong as expected. A more comprehensive school curriculum on tobacco prevention and control is recommended to reinforce antismoking messages among school children.
Chu Van Men,강종성,장유선,Kwan Jun Lee,이제현,TRANHONG QUANG,Nguyen Van Long,Hoang Van Luong,김영호 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12
A quantitative and pattern recognition analyses were conducted for quality evaluation of Kalopanacis Cortex (KC) using HPLC. For quantitative analysis, four bioactive compounds, liriodendrin, pinoresinol O-β-D-glucopyranoside, acanthoside B and kalopanaxin B, were determined. The analysis method was optimized and validated using ODS column with mobile phase of methanol and aqueous phosphoric acid. The validation gave acceptable linearities (r > 0.9995), recoveries (98.4% to 101.9%) and precisions (RSD < 2.20). The limit of detection of compounds ranged from 0.4 to 0.9 μg/mL. Among the four compounds, liriodendrin was recommended as a marker compound for the quality control of KC. The pattern analysis was successfully carried out by analyzing thirty two samples from four species, and the authentic KC samples were completely discriminated from other inauthentic species by linear discriminant analysis. The results indicated that the method was suitable for the quantitative analysis of liriodendrin and the quality evaluation of KC.
Anti-inflammatory compounds from Ampelopsis cantoniensis.
Van Thu, Nguyen,Cuong, To Dao,Hung, Tran Manh,Van Luong, Hoang,Woo, Mi Hee,Choi, Jae Su,Lee, Jeong-Hyung,Kim, Jeong Ah,Min, Byung Sun Natural Product Communications 2015 Natural product communications Vol.10 No.3
<P>Many natural products have been shown to have an inhibitory effect on nitric oxide (NO), and are used as chemotherapy agents for inflammation disease. The current study was designed to evaluate the anti-inflammatory activity of chemical components from the leaves of Ampelopsis cantoniensis. Sixteen compounds (1-16) were isolated and identified. Phloretin (5) and 5,7,3',5'-tetrahydroxyflavanone (16) inhibited nitric oxide (NO) production with IC50 values of 5.2, and 18.5 μM, respectively. The inhibitory effect of compounds 5 and 16 were accompanied by dose-dependent decreases in LPS-induced nitric oxide synthase (iNOS) in RAW 264.7 cells, respectively. This study investigated the significant anti-inflammatory properties of isolated compounds from the leaves of A. cantoniensis for the first time. The findings demonstrate that A. cantoniensis could be used beneficially in the treatment of inflammation disease.</P>
Applications of Magnetite Nanoparticles for Water Treatment and for DNA and Cell Separation
Nguyen Hoang Hai,Nguyen Chau,Nguyen Hoang Luong,Nguyen Thi Van Anh,Phan Tuan Nghia 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.3
Magnetic nanoparticles with a diameter of 15 nm prepared by using the coprecipitation method have been applied to enforce the sedimentation of the solid waste, to adsorb the arsenic ions in water, to increase the DNA concentration by using a magnetic eld for the electrochemical DNA sensor and to separate the helper CD4+ T cells to determine the number of the cells in blood. A combination of magnetic nanoparticles and alum makes the solid waste in water under a magnetic field aggregate a dozen times faster than under the gravity alone. A concentration of 0.1 mg/l of arsenic in water was reduced to a value lower than the permissible concentration of 0.01 mg/l after few minutes of stirring. The particles functionalized with 3-aminopropyl triethoxysilane were used to enrich the DNA of the Herpes virus, which extended the sensitivity of an electrochemical sensor down to a concentration lower than nM/l. The particles coated with fluorescent-labeled antiCD4 antibody were used to count the helper CD4+ T cells. The fluorescence signals of the particle/cell system were two times stronger than those of the uorescence antiCD4 cell system. This can be used for the treatment of an HIV-infected patient with a simple fluorescent microscope. Magnetic nanoparticles with a diameter of 15 nm prepared by using the coprecipitation method have been applied to enforce the sedimentation of the solid waste, to adsorb the arsenic ions in water, to increase the DNA concentration by using a magnetic eld for the electrochemical DNA sensor and to separate the helper CD4+ T cells to determine the number of the cells in blood. A combination of magnetic nanoparticles and alum makes the solid waste in water under a magnetic field aggregate a dozen times faster than under the gravity alone. A concentration of 0.1 mg/l of arsenic in water was reduced to a value lower than the permissible concentration of 0.01 mg/l after few minutes of stirring. The particles functionalized with 3-aminopropyl triethoxysilane were used to enrich the DNA of the Herpes virus, which extended the sensitivity of an electrochemical sensor down to a concentration lower than nM/l. The particles coated with fluorescent-labeled antiCD4 antibody were used to count the helper CD4+ T cells. The fluorescence signals of the particle/cell system were two times stronger than those of the uorescence antiCD4 cell system. This can be used for the treatment of an HIV-infected patient with a simple fluorescent microscope.
Duc Manh Hoang,Trinh Nam Trung,Phan Thi Thu Hien,Do Thi Ha,Hoang Van Luong,이명숙,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
Protein tyrosine phosphatase 1B (PTP1B), a negative regulator of insulin signaling, has served as a potential drug target for the treatment of type 2 diabetes. The MeOH extracts of twenty-nine medicinal plants, traditionally used in Vietnam as anti-diabetes agents, were investigated for PTP1B inhibitory activity in vitro. The results indicated that, most materials showed moderate to strong inhibitory activity with IC50 values ranging from 3.4 mg/mL to 35.1 mg/mL; meanwhile, eleven extracts (37.9%) could demonstrate PTP1B activity with IC50 values less than 15.5 mg/mL; sixteen extracts (55.2%) could demonstrate PTP1B activity with IC50 values ranging from 15.5 mg/mL to 35.1 mg/mL. The study may provide a proof, at least in a part, for the ethno-medical use in diabetes disease of these plants.
Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells
Duc Manh Hoang,Trinh Nam Trung,Long He,Do Thi Ha,이명숙,김보연,Hoang Van Luong,안종석,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.2
We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, a- and b-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of 80 mM, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with a- and b-eudesmol of 80 mM. Thus, both a- and b-eudesmol exerted caspase-3 activation and cytochrome c release at 80 mM in HL-60 cells. These results are firstly reported that the sesquiterpenes, a- and b-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.
Inverse MR and Dual-AMR Phenomena in Co/CoO/Ag/Co Sandwiches
Nguyen Anh Tuan,Luong Van Su,Hoang Quoc Khanh,Nguyen Anh Tue,Nguyen Thi Luyen 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.72 No.7
Band-form Co/CoO/Ag/Co sandwiches were prepared using radio-frequency magnetron sputtering. The sandwiches had rather thick Co (tCO = 63 nm) and Ag (tAg = 2 ÷ 65 nm) layers and a super-thin paramagnetic cobalt monoxide (CoO) layer (< 1 nm) inserted in the Co/Ag interface. The sandwiches exhibited anomalous magnetoresistance (MR) behavior depending on the tAg value. Inverse magnetoresistance (IMR) effect was observed only at thin tAg values of 2 nm and 6 nm, whereas the dual-anisotropic MR (dual-AMR) occurred at thicker tAg values of 12 nm, 25 nm and 65 nm. A superposition of the dual-AMR and the weak IMR effects was obtained at the thickest Ag layer of 65 nm. The origin of these anomalous MR behaviors was discussed to show the prominent role of the CoO layer and large thickness of the Co and Ag layers.
Methodology for the Global Youth Tobacco Use Survey (GYST), Vietnam, 2014
Kim, Bao Giang,Hoang, Van Minh,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Thuy Linh,Nguyen, Tuan Lam,Pham, Thi Quynh Nga,Luu, Ngoc Hoat Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
Viet Nam is a country with the highest rate of adult male smokers in the world. In 2010, the percentage of adult male smokers was 47.4%. Each year in Viet Nam, there are 40,000 lives lost from tobacco-related diseases. The Global Tobacco Youth Survey is an international standardized survey that has been conducted in more than 140 countries. To provide evidence for tobacco control, Viet Nam already conducted two rounds of GYTS in 2003 and 2007. The GYTS in 2014 is the survey's third iteration. This paper aims to document key methodological details and socio-demographic characteristics of the respondents included in the 2014 GYTS in Viet Nam. This survey followed international protocol and was conducted in 13 provinces representative of 6 geographical regions. A total of 3,430 school children, aged 13 to 15 years used a standardized answer sheet to answer 76 questions about seven tobacco-related topics, including prevalence of tobacco use, environmental tobacco smoke, access and availability, media and advertisement, cessation, knowledge and attitudes, tobacco-related school curriculum. This GYTS provides valid and reliable data for monitoring tobacco use among youth in Vietnam and is recommended to be regularly repeated.