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Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Nguyen Viet Than,Thipthaviphone Soulinho,Nguyen Hai Nam,Tran Thi Phuong,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-a (TNF-a), the mRNA expression levels of pro-inflammatory cytokines, including TNF-a and IL-1b, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-kB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.
Duc Manh Hoang,Trinh Nam Trung,Phan Thi Thu Hien,Do Thi Ha,Hoang Van Luong,이명숙,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
Protein tyrosine phosphatase 1B (PTP1B), a negative regulator of insulin signaling, has served as a potential drug target for the treatment of type 2 diabetes. The MeOH extracts of twenty-nine medicinal plants, traditionally used in Vietnam as anti-diabetes agents, were investigated for PTP1B inhibitory activity in vitro. The results indicated that, most materials showed moderate to strong inhibitory activity with IC50 values ranging from 3.4 mg/mL to 35.1 mg/mL; meanwhile, eleven extracts (37.9%) could demonstrate PTP1B activity with IC50 values less than 15.5 mg/mL; sixteen extracts (55.2%) could demonstrate PTP1B activity with IC50 values ranging from 15.5 mg/mL to 35.1 mg/mL. The study may provide a proof, at least in a part, for the ethno-medical use in diabetes disease of these plants.
Screening of Vietnamese Medicinal Plants for Cytotoxic Activity
Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Tran Viet Hung,Tran Thi Hien,Yim Namhui,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.1
Thirty-two methanol extracts of thirty-one Vietnamese medicinal plants were evaluated for the cytotoxic activity against five human cancer cell lines, including A549, MCF-7, HT 1080, Huh-7, and HepG2. Of these, the nine extracts of Acanthopanax trifoliatus (4), Acanthopanax gracilistylus (5), Siegesbeckia orientalis (10), Betula alnoides (11), Passiflora edulis (18), Zanthoxylum simulans (leaf, 23), Adenosma caeruleum (26), Solanum verbascifolium (29), and Alpinia malaccensis (31), exhibited high potent cytotoxic activity showing a certain degree of selectivity against the different cell types, with IC50 values ranging from 2.1 to 3.8 mg/mL.
Do Thi Ha,Trinh Nam Trung,Nguyen Hai Nam,Chu Van Men,Nguyen Bich Thu,Tran Thi Phuong,KiHwan Bae 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.6
The aim of the present study was to investigate effects of the ethyl acetate fraction of an ethanol extract of Coix lachryma-jobi (ECLJ) on glucose uptake and adipocyte differentiation in 3T3-L1 cells. ECLJ phosphorylated AMP-activated protein kinase (AMPK) and its downstream substrate acetyl-coenzymeA carboxylase in 3T3-L1 cells in a time- and dosedependent manner. Moreover, we discovered that compound C inhibits ECLJ-stimulated ACC phosphorylation. In addition,ECLJ exhibited a dose-dependent stimulation of glucose uptake in 3T3-L1 cells, and this increase was obviously attenuated by compound C. ECLJ also caused a decrease in the expression levels of adipogenesis factors such as fatty acid synthase, sterolregulatory-element-binding protein-1c, peroxisome proliferator-activated receptor g, and CAATT=enhancer binding protein a in a dose-dependent manner. Differentiation was examined by Oil red O staining activity after ECLJ treatment for 6 days. ECLJ decreased mean droplet size. These results suggest a possible role for AMPK in the process of adipose differentiation and that ECLJ targeted for adipocyte functions could be effective in improving the symptoms of metabolic syndrome.
홍지영,정화진,배송이,Trinh Nam Trung,배기환,이상국 대한암예방학회 2014 Journal of cancer prevention Vol.19 No.4
Background:Physcion is an anthraquinone from rhubarb (rhizomes of Rheum tanguticum) and has been reported to haveanti-inflammatory, hepatoprotective, antifungal, and anti-cancer activities. However, the growth inhibitory activity against human cancercells and the underlying molecular mechanisms have been poorly determined. This study was designed to investigate the anti-proliferativeactivity of physcion by induction of cell cycle arrest and apoptosis in human MDA-MB-231 triple negative breast cancer cell line. Methods:MDA-MB-231 cells were treated with physcion, and the anti-proliferative activity was evaluated by the sulforhodamine B assay. The mechanisms of action for the growth inhibitory activity of physcion were evaluated by flow cytometry for cell cycle distribution,and by Western blot for the assessment of potential target proteins. Results:Physcion showed a significant anti-proliferative activity against MDA-MB-231 human breast cancer cells. Flow cytometric analysisindicated that physcion markedly induced the accumulation of cells in the G0/G1 phase and the increase of cell population in the sub-G1phase. The G0/G1 cell cycle arrest by physcion was associated with the down-regulation of Cyclin D1, Cyclin A, CDK4, CDK2, c-Mycand phosphorylated Rb protein expressions. The increase of sub-G1 peak by physcion was closely correlated with the induction ofapoptosis,which was confirmed by the induction of cleaved poly-(adenosine diphosphate ribose) polymerase, activation of Caspases, and suppressionof Bid and Bcl-2 expression. Conclusions:The induction of G0/G1 cell cycle arrest and apoptosis might be one of the plausible mechanisms of actions for theanti-proliferative activity of physcion in human breast cancer cells.
Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells
Duc Manh Hoang,Trinh Nam Trung,Long He,Do Thi Ha,이명숙,김보연,Hoang Van Luong,안종석,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.2
We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, a- and b-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of 80 mM, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with a- and b-eudesmol of 80 mM. Thus, both a- and b-eudesmol exerted caspase-3 activation and cytochrome c release at 80 mM in HL-60 cells. These results are firstly reported that the sesquiterpenes, a- and b-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.
Lipoxygenase Inhibitory and Antioxidant Activities of Isolated Compounds from Moutan Cortex
Do Thi Ha,Trinh Nam Trung,Nguyen Duy Thuan,NamHui Yim,민병선,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.2
Phytochemical investigation on the ethyl acetate and n-butanol fractions of Moutan Cortex resulted in the isolation and characterization of a new monoterpene glycoside (3) and twenty known monoterpene glycosides (1, 2, 4 - 21). The structure of 3 was determined by spectroscopic data interpretation and physico-chemical properties. Compounds 1 and 8 presented a remarkable inhibitory activity against lipoxygenase-1 (LOX-1) with IC50 values of 45.2 and 37.5 mM, respectively. Compounds 9, 10, 13, 18, 19, and 21 showed significant 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect with IC50 values of 9.8, 25.5, 6.4, 15.2, 18.7, and 23.7 ?M, respectively. Benzoylpaeoniflorin (8), which exhibited the highest inhibitory effect with an IC50 value of 37.5 ? 0.7 mM, was further analyzed the inhibition kinetics by Lineweaver-Burk plots. Results indicated that 8 is a non-competitive inhibitor, and the kinetic parameter values were estimated to be (31.04 mM, Ki), (0.29 mM/min, Vm), and (48.50 mM, Km).