http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
신희봉,이경원,윤지훈,이양순,김명숙 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.2
Purpose: The genus Aeromonas is a pathogen that is well known to cause severe clinical illnesses, ranging from gastroenteritis to sepsis. Accurate identification of A. hydrophila, A. caviae, and A. veronii is important for the care of patients. However, species identification remains difficult using conventional methods. The aim of this study was to compare the accuracy of different methods of identifying Aeromonas at the species level: a biochemical method, matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS), 16S rRNA sequencing, and housekeeping gene sequencing (gyrB, rpoB). Materials and Methods: We analyzed65 Aeromonas isolates recovered from patients at a university hospital in Koreabetween 1996 and 2012. The isolates were recovered from frozen states and testedusing the following four methods: a conventional biochemical method, 16S rRNA sequencing, housekeeping gene sequencing with phylogenetic analysis, and MALDI-TOF MS. Results: The conventional biochemical method and 16S rRNA sequencing identified Aeromonas at the genus level very accurately, although species level identification was unsatisfactory. MALDI-TOF MS system correctly identified 60 (92.3%) isolates at the species level and an additional four (6.2%) at the genus level. Overall, housekeeping gene sequencing with phylogenetic analysis was found to be the most accurate in identifying Aeromonas at the species level. Conclusion: The most accurate method of identification of Aeromonas to species level is by housekeeping gene sequencing, although high cost and technical difficulty hinder its usage in clinical settings. An easy-to-use identification method is needed for clinical laboratories, for which MALDI-TOF MS could be a strong candidate.
지역사회 관련 메티실린내성 황색포도알균에 의한 균혈증 2예
김정현,강은정,정윤석,전민혁,김태형,신희봉,박수진,추은주 대한감염학회 2009 감염과 화학요법 Vol.41 No.1
Recent reports have described an increasing incidence of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) in patients who do not exhibit established risk factors for healthcare exposure. We report two cases of CA-MRSA bacteremia complicated by vertebral osteomyelitis. Both of CA-MRSA isolates were resistant to beta-lactam agents, but susceptible to clindamycin, trimethoprim-sulfamethoxazole, gentamicin, ciprofloxacin, and tetracycline. Both isolates carried staphylococcal cassette chromosome mec (SCCmec) type IVA, and were identified as sequence type (ST) 72 by mlultilocus sequence typing (MLST). However, the Panton-Valentine leukocidin (PVL) gene was not identified.
Brevundimonas diminuta의 가성유행(Pseudo-outbreak)
김용현,신희봉,고은미,이용화,이유경,이경원 대한임상미생물학회 2011 Annals of clinical microbiology Vol.14 No.3
Brevundimonas diminuta is a lactose non-fermenting gram-negative rod and associated with infection of immunocompromised patient. In three patients of two general wards, B. diminuta was isolated in blood culture sample. Their clinical feature doesn’t coincide with blood culture result and pseudo-outbreak was suspected. These isolated were identified as Brevundimonas diminuta, biochemically, and 16S rRNA sequencing result reassured their identification. The PFGE result showed single pattern and their clonality was assumed.