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Seul Chan Park,Seong-Kyoon Choi,Se-Hyeon Han,Song Park,Hye Jin Jeon,Seung Chan Lee,Kyeong Yeon Kim,Young Seo Lee,김지형,Jee Eun Han 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.2
In this study, whiteleg shrimp (Penaeus vannamei) imported from Vietnam were collected from South Korean markets, and examined for 2 viruses: infectious hypodermal and hematopoietic necrosis virus (IHHNV, recently classified as decapod penstyldensovirus-1), and white spot syndrome virus (WSSV). Among 58 samples, we detected IHHNV in 23 samples and WSSV in 2 samples, using polymerase chain reaction and sequencing analyses. This is the first report of IHHNV and WSSV detection in imported shrimp, suggesting that greater awareness and stricter quarantine policies regarding viruses infecting shrimp imported to South Korea are required.
The Limitation of Making Hepatic Fibrosis in NAFLD Animal Model
( Han Seul Park ),( Jae Young Jang ),( Soung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( Boo Sung Kim ),( So Young Jin ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Animal models of NAFLD give crucial information, not only pathogenesis of NAFLD but also therapeutic effects of various agents. We investigated the degree of steatosis, inflammation and fibrosis of the liver obtained from three animal models of NAFLD with different mouse species and diets. Methods: Group1: The mice were fed a control diet. Group2: The gene responsible for the production of leptin deficient ob/ob mice fed a 60% fat diet. Group3: The leptin receptor deficient db/db mice were fed a diet deficient in methionine and choline (MCD) diet. Group4: The leptin receptor deficient db/db mice were fed a 60% fat + 2.5% cholesterol diet and drinking water (55% fructose, 45% glucose w/w). Change of body weight was observed and blood was collected before sacrifice. After being sacrificed, the liver tissues were collected and fixed in formalin. Histological evaluation was conducted by blindly pathologist. Results: The body weight in group1 & 2 & 4 (control & high-fat-diet fed mice) were increased during the study, whereas group3 (MCD feeding mice) showed weight loss for unclear reasons. Liver histology showed no significant difference in hepatic steatosis and inflammation among the three groups (group2-4). Meanwhile, liver fibrosis was slightly more frequent in group3 than that in groups2 and 4. However, the maximum degree of fibrosis in group3 was Ib. The level of liver enzyme showed no significant difference, whereas the level of triglyceride was significantly increased in group4 (TG: 30.8±3.2 (group1), 31.8±4.9 (group2), 32.8±1.7 (group3), 141±15.20 (group4) (p < 0.001) (mg/dL)). Conclusions: The results show that the three animal models are thought to induce hepatic steatosis and inflammation very well, but the induction of hepatic fibrosis is still considered to be limited. A better animal model development or integration model would be needed.
Establishment of Hepatoma Treatment Model Using Hepatoma Cell Spheroids
( Han Seul Park ),( Jae Young Jang ),( Seoung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( Boo Sung Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Three dimensional (3D) spheroid cells are more closely mimic natural tissues and organs than cells grown in 2D. ``Biocompatible spheroidal hepatoma cell`` is thought to be closest model of real patient``s HCC. We have made spheroidal hepatoma cells using the proven technology and already confirmed apoptotic effect of ginsenoside Rh2 and Rg5 on 2D cells (Huh7 and Huh7.5.1). In this study, we investigate apoptotic effects of ginsenoside Rh2 and Rg5 using 3D hepatoma cell spheroids. Methods: Huh7 and Huh7.5.1 cells were maintained in culture dishes in RPMI supplemented with 10% inactivated fetal bovine serum (FBS) and DMEM supplemented with 10 % dialyzed FBS, respectively. When they reached about 80 % confluence, cells were harvested from 2-D petri-dish cultures by treatment with trypsin. These cells cultured in 1.5 % soft agarose gels for 10-14 days. After 10-14 days, 3-D hepatic structure was formed and treated with ginsenoside Rh2 (100, 200uM) and Rg5 (10, 50, 100uM) for 72h. Comparison between 2-D and 3-D models was done with microscopic and protein analysis. Results: The behaviors of 2D and 3D cells (Huh7 and Huh7.5.1, respectively) have been shown different morphologic change. 3-D culture of Huh7 and Huh7.5.1 cells had a longer survival time rather than 2-D cell model. The response to ginsenoside Rh2 and Rg5 on 3D culture systems showed a lower cell death rate compared with 2D culture systems. The expression of cleaved PARP protein was increased in both 2D and 3D cells with exposure to ginsenoside Rh2 and Rg5. Conclusions: Hepatoma cell sheroids had a longer survival time and a similar apoptotic effect compared to 2-D cell model in drug screening. It is expected to have an important role on the drug screening and treatment prediction in HCC.
Han Seul Cho,Woo Joon Park,Go Eun Hong,Ji Han Kim,Min Gu Ju,Chi Ho Lee 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.1
This study was performed to assess the antioxidant activity of Allium hookeri root extract (AHE) on lipid oxidation of raw sulfur-fed pork patties for 14 d of refrigerated storage. Different concentration of ethanol (0-100%) and time (1-12 h) were applied to determine the extraction condition. Water (0% ethanol) extraction for 1 h was selected as an optimal extraction condition of AHE for the following study showing the highest total phenolic content and total flavonoid content, as well as the strongest antioxidant activity. The 1% AHE (SP1), 3% AHE (SP2), and 0.05% ascorbic acid (SP3) were added into sulfur- fed pork patties against controls; SP0 (sulfur-fed pork patties with no AHE) and P0 (normal pork patties with no AHE). The pH values of P0 and SP0 significantly increased (p<0.05) than others on 14 d and redness of P0 showed the largest decrement during storage. P0 and SP0 showed higher production of conjugated dienes on d 7 than others (p<0.05). Thiobarbituric acid reactive substances (TBARS) values were decreased in proportion to the increased level of AHE on 14 d (p< 0.05) resulting in higher TBARS values on P0 and SP0 (p<0.05) and the negative correlation between AHE level and TBARS were also demonstrated (r=-0.910, p=0.001). Therefore, the results suggest that AHE effectively retarded the lipid oxidation rate of sulfur-fed pork patties indicating the potential usage of AHE as a natural preservative.
Neurodevelopmental Outcomes and Brain Volumetric Analysis of Low-Grade Intraventricular Hemorrhage
Seul Gi Park,Hyo Ju Yang,Soo Yeon Lim,Seh Hyun Kim,Seung Han Shin,Ee Kyung Kim,Han Suk Kim 대한신생아학회 2023 Neonatal medicine Vol.30 No.2
Purpose: Extremely preterm infants are prone to brain injury and underdevelopment. Intraventricular hemorrhage (IVH) is the most common cause of brain injury and a significant risk factor for neurodevelopmental delay in preterm infants. Severe IVH is known to have a poor outcome; however, the outcomes of low-grade IVH remain controversial. This study aimed to evaluate neurodevelopmental outcomes and brain segmental volumes of preterm infants with low-grade IVH. Methods: This retrospective cohort study included 109 extremely preterm infants who underwent term equivalent age-magnetic resonance imaging and neurodevelopmental evaluation at a corrected age of 18 to 24 months. We compared infants with and without low-grade IVH. Results: Among the 109 extremely preterm infants, 25 had low-grade IVH and 84 had no IVH. There were no significant differences in the neurodevelopmental outcomes between the low-grade and no IVH groups. In multivariate analysis, low-grade IVH was associated with a smaller medullary volume (adjusted odds ratio, 0.575; 95% confidence interval, 0.346 to 0.957; P=0.034). Conclusion: We found no significant differences in the neurodevelopmental outcomes of extremely preterm infants at a corrected age of 18 to 24 months between those with low-grade IVH and those without IVH. Low-grade IVH was associated with a smaller medullary volume.
Alopecia areata in a spayed pug: clinical and immunohistochemical findings
Park, Seul-Gy,Lee, Chang-Min,Han, Jae-Ik,Kim, Seung-Gon,Kim, Ha-Jung,Park, Hee-Myung The Korean Society of Veterinary Science 2015 大韓獸醫學會誌 Vol.55 No.4
A two-year-old spayed female pug presented with symmetrical hyperpigmented alopecic lesions on her axillary and inguinal regions. There were no remarkable findings in dermatologic examinations and hormonal assays. Histological examination of biopsied tissues revealed prominent lymphocytic perifolliculitis along with shrunk hair follicles. Immunohistochemistry for CD3, CD79a, CD4, and CD8 showed a positive stain for CD4 antigen around hair bulbs, suggesting CD4 positive T lymphocyte infiltration. This case suggests the possibility that CD4 T lymphocyte-mediated inflammatory reaction could be a main mechanism in canine alopecia areata. Additional studies are warranted to investigate the immunological mechanism in canine species.
( Han Seul Park ),( Jae Young Jang ),( Soung Won Jeong ),( Jeong-ju Yoo ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( So Young Jin ),( 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Animal & cell models of hepatoma give a crucial information, not only pathogenesis of liver cancer but also therapeutic effects of various agents. In this study, we investigate therapeutic effects of ginsenoside Rh2 and Rg5 using animal & cell models of hepatoma comparing with sorafenib. Methods: Huh7 & huh7.5.1 cells were harvested from 2-D petri-dish and cultured in 1.5 % soft agarose gels for 10-14 days. After 10-14 days, 3-D hepatic structure was formed and treated with ginsenoside Rh2 (100, 200uM) and Rg5 (10, 50, 100uM) for 72h. Hep3b cells (2×10<sup>5</sup>) in matrigel were suspended in 100 μl of phosphate-buffered saline (PBS) and then injected into the flanks of BALB/C nude mouse at 6 weeks. Sorafenib (10mg/kg), ginsenoside Rh2 (100 uM) and Rg5 (100 uM) was injected to intra-peritoneum twice a week. After 4 weeks, all mice were sacrificed and tumor tissue was collected. The tissue was stained with hematoxylin and eosin and histological evaluation was conducted by blindly pathologist. Area of necrosis and vascular change in tumor tissue was calculated using the lasso tool to encircle the area in ZEN 2011 Imaging Software. Results: Both ginsenoside Rh2 and Rg5 induced cell necrosis in hepatocellular carcinoma (HCC) cell lines, and more necrosis occurred in 2D models. The expression of cleaved PARP protein was increased in both 2D and 3D cells with exposure to ginsenoside Rh2 and Rg5. The hepatocellular carcinoma was confirmed in hepatoma mouse models by H&E stain. Increased necrosis and telangiectasia were observed in mice treated with sorafenib, Rh2, and Rg5 compared to control mouse. Conclusions: Our findings provide insight into the use of xenograft mouse as models of HCC and ginsenoside Rh2 & Rg5 might be a potential treatment candidate of liver cancer.
The Inhibitory Effect of Lorcaserin on Non Alcoholic Fatty Liver Disease in Animal Model
( Han Seul Park ),( Jae Young Jang ),( Seoung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( Boo Sung Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Nonalcoholic fatty liver disease (NAFLD) is characterized by a wide spectrum of liver damage spanning steatosis, nonalcoholic steatohepatitis, and liver cirrhosis. The aim of this study is to investigate the efficacy of lorcaserin on NAFLD in animal model. Methods: The leptin receptor deficient db/db mice and control mice (db/m) were fed a diet deficient in methionine and choline (MCD diet) and control diet for 8, respectively. Twenty mice were divided into 3 groups. The first group was fed a control diet without treatment and referred as the control group. The second group was administered with MCD diet +0.7% DMSO. The third group was administered with MCD diet +0.7% DMSO +5mg/ml of lorcaserin. Change of body weight was observed and blood was collected before sacrifice. After being sacrificed, the liver tissues were collected and fixed in formalin. Histological evaluation was evaluated by blindly pathologist. Results: The body weight of control mice was increased during the study, whereas feeding db/db mice MCD diets for 8 weeks significantly reduced in body weight. Lorcaserin treated group was associated with more rapid body weight loss compared with DMSO-treated controls. MCD diet induced excessive fat accumulation, inflammation, and some fibrosis. Liver enzyme and triglyceride were improved in lorcaserin-treated group compared with DMSO-treated control (DMSO vs lorcaserin: AST 411.3 ±40.26 vs 304.7 ±28.88 (U/L), ALT 544.8 ±38.7 vs 434.5 ±29.68 (U/L), triglyceride 31.8 ±2.02 vs 26 ±1.58 (mg/dL)). Liver histopathology showed that the fat accumulation and inflammatory cell infiltration were decreased in MCD diet +lorcaserin-treated mice compared with MCD diet +DMSO-treated controls. Conclusions: These results showed beneficial effects of lorcaserin against excessive fat accumulation and inflammation as well as liver enzyme. Therefore, our findings indicate that lorcaserin could be contributing to the decline of progression of nonalcoholic fatty liver disease.