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Jia-Jia Lin,Young-Hyun Han,Jung-Woo Kwon,Yong-Nan Xu,Yi-Bo Luo,Yu-Jin Jo,Chang-Eun Park,Jung-Kyu Baang,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s
In meiosis, Emi2 plays important role as CSF (Cytostatic Factor) to make the oocyte arrested in mII stage by the inhibition of APC/C (anaphase promoting complex/cyclosome). Once the oocyte fertilized, Emi2 was destabilized and degraded. For the degradation of Emi2, calcium signaling activate calmodulin-dependent protein kinase (CaMK) and phosphorylate emi2. Phosphorylated emi2 is recognized by polo-box domain of polo-like kinase 1 (Plk1) and further degradated by ubiquitin-dependent proteolysis. But recognition of Plk1 and emi2 is unknown. In this works, we determined the high-resolution crystal structure of polo-box domain of Plk1 and phosphorylated emi2 peptide at 1.90Å. Determined structure revealed that several unique features, including binding of Phe169 in the tyrosin-rich hydrophobic pocket. This is the first report of crystallization that Plk1-emi2 complex. Based on the complex structure, we designed the peptide analogs which pontentially inhibits recognition of Emi2 by Plk1 and assessed its biological activity in oocyte maturation and pathernogenetic activation. Injection of AB103-8, the inhibitor of Plk1 Polo-box domain, in mouse oocytes, induced the maturation arrest in GV stage and the delay in mII parthenogenetic activation. Further investigations of the mechanism that Plk1 involved into the Emi2 mII arrest are underway.
Park, Ji Young,Ji, Hyun Dong,Jeon, Bo Ra,Im, Eun Ju,Son, Young Min,Lee, Joo Young,Lee, Dong-Ha,Lee, Young-Chul,Hyun, Eujin,Jia, Qi,Hong, Mei,Park, Hwa-Jin,Rhee, Man Hee Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>A number of reagents that prevent thrombosis have been developed but were found to have serious side effects. Therefore, we sought to identify complementary and alternative medicinal materials that are safe and have long-term efficacy. In the present studies, we have assessed the ability of chlorine e6 (CE6) to inhibit ADP-induced aggregation of rat platelets and elucidated the underlying mechanism. CE6 inhibited platelet aggregation induced by 10 <I>µ</I>M ADP in a concentration-dependent manner and decreased intracellular calcium mobilization and granule secretion (i.e., ATP and serotonin release). Western blotting revealed that CE6 strongly inhibited the phosphorylations of PI3K, Akt, c-Jun N-terminal kinase (JNK), and different mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase 1/2 (ERK1/2) as well as p38-MAPK. Our study also demonstrated that CE6 significantly elevated intracellular cAMP levels and decreased thromboxane A<SUB>2</SUB> formation in a concentration-dependent manner. Furthermore, we determined that CE6 initiated the activation of PKA, an effector of cAMP. Taken together, our findings indicate that CE6 may inhibit ADP-induced platelet activation by elevating cAMP levels and suppressing PI3K/Akt activity. Finally, these results suggest that CE6 could be developed as therapeutic agent that helps prevent thrombosis and ischemia.</P>
Park, Joung-Man,Wang, Zuo-Jia,Kwon, Dong-Jun,Gu, Ga-Young,Lee, Woo-Il,Park, Jong-Kyoo,DeVries, K. Lawrence Elsevier 2012 Composites. Part B, Engineering Vol.43 No.3
<P><B>Abstract</B></P> <P>Interfacial and other properties along with self-sensing were investigated for single carbon fiber/neat phenolic resins and carbon nanotube (CNT)-phenolic nanocomposites by electro-micromechanical and wettability tests. The apparent modulus was higher for samples with a single carbon fiber in CNT-phenolic nanocomposite than for samples with a single carbon fiber in neat phenolic resin, indicating better stress transfer. In water droplet contact angle measurements the contact angle increased form slightly less than 90° on neat phenolic resin to more than 90° on CNT-phenolic nanocomposites. This behavior was attributed to hydrophobic domains randomly distributed on the surface as a result of the heterogeneous microstructure of CNT. The work of adhesion between a single carbon fiber and CNT-phenolic nanocomposites was greater than for neat phenolic resin which is attributed to an increase in viscosity by adding CNT. Micro-failure patterns and interfacial adhesion between CNT-phenolic nanocomposites and single carbon fibers were consistent with these other results.</P>
Jia, Yuefa,Liu, Jia,Cha, Sangwon,Choi, Soobin,Park, Yun Chang,Liu, Chunli THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2017 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.47 No.-
<P><B>Abstract</B></P> <P>Magnetically recyclable Au-TiO<SUB>2</SUB>/nanocube ZnFe<SUB>2</SUB>O<SUB>4</SUB> (Au-TiO<SUB>2</SUB>/NC ZFO) composite photocatalysts have been successfully prepared by a hydrothermal method. It was revealed that Au-TiO<SUB>2</SUB>/NC ZFO composite with 25% mass percentage of Au-TiO<SUB>2</SUB> exhibited a significantly enhanced photocatalytic efficiency toward chlortetracycline (CTC) degradation as compared to the binary TiO<SUB>2</SUB>/NC ZFO composite. The enhanced performance can be attributed to the addition of Au nanoparticles, which act as electron traps aiding the electron-hole separation and increasing the light absorption through the surface plasmon resonance effect. The synthesized catalyst showed good stability after three cycles and could be easily separated by a magnet and reused.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new type ternary Au-TiO<SUB>2</SUB>/nanocube ZnFe<SUB>2</SUB>O<SUB>4</SUB>. </LI> <LI> The Au-TiO<SUB>2</SUB>/nanocube ZnFe<SUB>2</SUB>O<SUB>4</SUB> shows superior photocatalytic activity. </LI> <LI> The chlortetracycline can be removed by Au-TiO<SUB>2</SUB>/nanocube ZnFe<SUB>2</SUB>O<SUB>4</SUB>. </LI> <LI> Cycle degradation tests show the catalyst was highly active, stable and recoverable. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Magnetically separable Au-TiO<SUB>2</SUB>/nanocube ZnFe<SUB>2</SUB>O<SUB>4</SUB> composite exhibit an outstanding photocatalytic activity in degradation of chlortetracycline (CTC) under visible light irradiation.</P> <P>[DISPLAY OMISSION]</P>