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Infrared Camera Calibration in the 3D Temperature Field Reconstruction
Sun Xiaoming,Wu Haibin,Wang Wei,Liubo,Cui Guoguang 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.6
In order to reconstruct the 3D temperature field based on the binocular vision system, we carry out a research on the calibration of the infrared camera. To start with, we make a calibration board which can be clearly identified by the infrared camera; then, with the help of OpenCV, we implement the calibration of two infrared cameras respectively and rectify the infrared images. According to the parameters obtained from the single infrared camera calibration, we accomplish the stereo calibration. The experimental results show that the average reprojecting error is below 0.14 pixels and when using the system to carry out a real measurement, the average error is 3.12%. The feasibility of the proposed calibration approach is verified, the experimental measurement results is close to the ground truth.
Jian Fu,Haibin Sun,Yongzhe Zhang,Wei Xu,Chuan Wang,Yanwei Fang,Jianhui Zhao 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.1
Luteolin (LU) is a widely distributed flavonoid with multitarget effects. The objective of this study was to determine whether LU could reduce the ischemia–reperfusion injury of the spinal cord (SCII) in a rat model. Forty-eight rats were divided into four groups: sham, SCII, SCII+L-LU (50 mg/kg), and SCII+H-LU (100 mg/kg). Abdominal aortic occlusion was carried out for 40 min in all groups. Hindlimb motor functions were evaluated using the Tarlov scoring system. Nissl and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) staining were used to detect cell survival and apoptosis in the spinal cord. Spinal cord samples were taken for determination of malondialdehyde, xanthine oxidase, superoxide dismutase, and glutathione peroxidase activities. The levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-18 were assessed using ELISA kits to examine the inflammatory responses in the spinal cord. Western blot analysis was used to examine the expression of nuclear factor erythroid 2-related factor (Nrf2) and nod-like receptor pyrin domain-containing 3 protein (NLRP3) levels. We found that LU pretreatment significantly improved the locomotor function of rats after SCII, increased neuron survival, and inhibited apoptosis in the spinal cord. Furthermore, the oxidative stress and inflammatory response were significantly suppressed upon treatment with LU. Finally, LU upregulated Nrf2 levels and downregulated NLRP3 protein expression in SCII tissues. Thus, LU exhibited a neuroprotective effect following SCII by alleviating oxidative stress and inhibiting inflammatory responses and cell apoptosis. The possible mechanism may be related to the activation of Nrf2 and inhibition of NLRP3 inflammasome pathway.
Yitong Zhou,Haibin Sun 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.11
In this paper, the problem of adaptive static output feedback anti-disturbance quantized control is proposed for nonlinear systems with multi-source disturbances and unknown parameters. Using the output information, the disturbance observer and the adaptive law are constructed. An adaptive static output feedback anti-disturbance quantized controller is designed by combining with the estimated values of the disturbance and unknown parameters, in which a compensation term is introduced to eliminate the influence of quantization error. The stability andH∞ performance are analyzed by using Lyapunov function method. Finally, a simulation example is given to verify the validity of the results.
Shi Si,Gu Huijie,Xu Jinyuan,Sun Wan,Liu Caiyin,Zhu Tong,Wang Juan,Gao Furong,Zhang Jieping,Ou Qingjian,Jin Caixia,Xu Jingying,Chen Hao,Li Jiao,Xu Guotong,Tian Haibin,Lu Lixia 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Excessive osteoclast activation, which depends on dramatic changes in actin dynamics, causes osteoporosis (OP). The molecular mechanism of osteoclast activation in OP related to type 1 diabetes (T1D) remains unclear. Glia maturation factor beta (GMFB) is considered a growth and differentiation factor for both glia and neurons. Here, we demonstrated that Gmfb deficiency effectively ameliorated the phenotype of T1D-OP in rats by inhibiting osteoclast hyperactivity. In vitro assays showed that GMFB participated in osteoclast activation rather than proliferation. Gmfb deficiency did not affect osteoclast sealing zone (SZ) formation but effectively decreased the SZ area by decreasing actin depolymerization. When GMFB was overexpressed in Gmfb-deficient osteoclasts, the size of the SZ area was enlarged in a dose-dependent manner. Moreover, decreased actin depolymerization led to a decrease in nuclear G-actin, which activated MKL1/SRF-dependent gene transcription. We found that pro-osteoclastogenic factors (Mmp9 and Mmp14) were downregulated, while anti-osteoclastogenic factors (Cftr and Fhl2) were upregulated in Gmfb KO osteoclasts. A GMFB inhibitor, DS-30, targeting the binding site of GMFB and Arp2/3, was obtained. Biocore analysis revealed a high affinity between DS-30 and GMFB in a dose-dependent manner. As expected, DS-30 strongly suppressed osteoclast hyperactivity in vivo and in vitro. In conclusion, our work identified a new therapeutic strategy for T1D-OP treatment. The discovery of GMFB inhibitors will contribute to translational research on T1D-OP.
Stability Analysis of Switched Delay Systems with All Subsystems Unstable
Guang-Deng Zong,Qingzhi Wang,Haibin Sun 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.5
The stability problem of switched delay systems with all subsystems unstable is investigated in this paper. A sufficient criterion is firstly proposed to guarantee asymptomatic stability of nonlinear switched delay systemswith all subsystems unstable, where the stabilization property of switching behaviors is exploited to compensate thedivergence of states. Then by constructing multiple discretized Lyapunov-Krasovskii functionals, stability criteriaare developed for linear switched time-invariant and time-varying delay systems with all subsystems unstable. Finally, two examples are provided to illustrate the feasibility, superiority and application of the proposed approach.
Jian Fu,Wei Xu,Yongzhe Zhang,Haibin Sun,Jianhui Zhao 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.3
Spinal cord ischemia–reperfusion injury (SCII) easily causes unalterable neurological deficits. We previously demonstrated that the flavonoid luteolin (LU) has strong antioxidant, anti-inflammatory, and other neuroprotective efficacies against SCII. In our current study, we examined the contributions of the NF-E2-related factor 2 (Nrf2)/glutamate–cysteine ligase (GCL) pathway to LU-mediated neuroprotection in the transient abdominal aorta occlusion rat model of SCII. Rats were divided into four groups: Sham surgery, SCII alone, SCII plus LU pretreatment (SCII + LU), and SCII plus cotreatment with LU and the Nrf2 inhibitor ML385 (SCII + LU + ML385). The Basso–Beattie–Bresnahan (BBB) scale was used to assess neurological function, hematoxylin and eosin staining to evaluate pathological change to the spinal cord, and enzyme-linked immunosorbent assay to measure tissue markers of oxidative stress and inflammation induced by SCII. Mitochondrial injury and apoptosis were examined by flow cytometry and expression levels of Nrf2, GCL catalytic subunit (GCLc), and GCL modifier subunit (GCLm) by real-time quantitative polymerase chain reaction. LU pretreatment significantly enhanced recovery of motor function as evidenced by the BBB score and attenuated the pathological damage. Furthermore, LU effectively enhanced the antioxidative activity, alleviated mitochondrial swelling, decreased the expression levels of several proinflammatory cytokines after SCII, and significantly upregulated Nrf2, GCLc, and GCLm expression levels. Cotreatment with ML385 reversed all these protective effects of LU except the anti-inflammatory response. Collectively, these findings indicate that the neuroprotective efficacy of LU depends on suppression of oxidative stress and preservation of mitochondrial function through signaling pathways involving Nrf2 activation and downstream gene expression.
Xiaojun Zhang,Wei Zhao,Chuanmin Li,Haibin Yu,YanYan Qiao,Aonan Li,Chunyan Lu,Zhihui Zhao,Boxing Sun 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.11
To verified the target genes of miR-34c, bioinformatics software was used to predict the targets of miR-34c. Three possible target genes of miR-34c related to spermatogenesis and male reproductive development: zinc finger protein 148 (ZNF148), kruppel-like factor 4 (KLF4), and platelet-derived growth factor receptor alpha (PDGFRA) were predicted. Then, the expression of miR-34c and its target genes were detected in swine testicular tissue at different developmental stages by quantitative polymerase chain reaction. The results suggested that the expression of PDGFRA has the highest negative correlation with miR-34c. Then immunohistochemical staining was done to observe the morphology of swine testicular tissue at 2-days and 3, 4, 5-months of age, which indicated that PDGFRA was mainly expressed in the support cells near the basement membrane during the early development stages of testicular tissue, but that the expression of PDGFRA was gradually reduced in later stages. Therefore, western blot analyzed that the highest expression of PDGFRA was generated in 2-days old testicular tissues and the expression levels reduced at 3 and 4-months old, which correlated with the results of immunohistochemical staining. In conclusion, PDGFRA is a target gene of miR-34c.
Zhang, Xiaojun,Zhao, Wei,Li, Chuanmin,Yu, Haibin,Qiao, YanYan,Li, Aonan,Lu, Chunyan,Zhao, Zhihui,Sun, Boxing Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.11
To verified the target genes of miR-34c, bioinformatics software was used to predict the targets of miR-34c. Three possible target genes of miR-34c related to spermatogenesis and male reproductive development: zinc finger protein 148 (ZNF148), kruppel-like factor 4 (KLF4), and platelet-derived growth factor receptor alpha (PDGFRA) were predicted. Then, the expression of miR-34c and its target genes were detected in swine testicular tissue at different developmental stages by quantitative polymerase chain reaction. The results suggested that the expression of PDGFRA has the highest negative correlation with miR-34c. Then immunohistochemical staining was done to observe the morphology of swine testicular tissue at 2-days and 3, 4, 5-months of age, which indicated that PDGFRA was mainly expressed in the support cells near the basement membrane during the early development stages of testicular tissue, but that the expression of PDGFRA was gradually reduced in later stages. Therefore, western blot analyzed that the highest expression of PDGFRA was generated in 2-days old testicular tissues and the expression levels reduced at 3 and 4-months old, which correlated with the results of immunohistochemical staining. In conclusion, PDGFRA is a target gene of miR-34c.