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Draft Genome Sequence of the Thermophilic Bacterium Anoxybacillus kamchatkensis G10
Lee, Sang-Jae,Lee, Yong-Jik,Ryu, Naeun,Park, Seulki,Jeong, Haeyoung,Lee, Sang Jun,Kim, Byoung-Chan,Lee, Dong-Woo,Lee, Han-Seung American Society for Microbiology 2012 Journal of Bacteriology Vol.194 No.23
<B>ABSTRACT</B><P>Anoxybacillus kamchatkensisG10 is a spore-forming thermophilic bacterium isolated from a hot spring in Indonesia. Here, we report the draft genome sequence ofA. kamchatkensisG10 that may reveal insights into aerobic/anaerobic metabolisms and carbon utilization in moderate thermophiles.</P>
Lee, Sang Kwang,Kim, Yongtae,Kim, Sung-Soo,Lee, Jeong Hwa,Cho, Kun,Lee, Sang Sook,Lee, Zee-Won,Kwon, Kyung-Hoon,Kim, Young Hye,Suh-Kim, Haeyoung,Yoo, Jong Shin,Park, Young Mok WILEY-VCH Verlag 2009 Proteomics Vol.9 No.18
<P>Mesenchymal stem cells (MSCs) are multipotent cells, which have the capability to differentiate into various mesenchymal tissues such as bone, cartilage, fat, tendon, muscle, and marrow stroma. However, they lose the capability of multi-lineage differentiation after several passages. It is known that basic fibroblast growth factor (bFGF) increases growth rate, differentiation potential, and morphological changes of MSCs in vitro. In this report, we have used 2-DE coupled to MS to identify differentially expressed proteins at the cell membrane level in MSCs growing in bFGF containing medium. The cell surface proteins isolated by the biotin–avidin affinity column were separated by 2-DE in triplicate experiments. A total of 15 differentially expressed proteins were identified by quadrupole-time of flight tandem MS. Nine of the proteins were upregulated and six proteins were downregulated in the MSCs cultured with bFGF containing medium. The expression level of three actin-related proteins, F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2, was confirmed by Western blot analysis. The results indicate that the expression levels of F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2 are important in bFGF-induced morphological change of MSCs.</P>
Lee, Haeyoung,Han, Sang-Ok,Ryoo, Sang-Boom,Lee, Jeong-Soon,Lee, Gang-Woong Copernicus GmbH 2019 Atmospheric chemistry and physics Vol.19 No.4
<P>Abstract. To understand the carbon cycle at policy-relevant spatial scales, a high density of high-quality CO2 measurement sites is needed. In 2012, the Korea Meteorological Administration (KMA) installed CO2 monitoring systems at Anmyeondo (AMY) in the west, Jejudo Gosan Suwolbong (JGS) in the southwest, and Ulleungdo (ULD) in the east of South Korea. Three stations were instrumented with identical greenhouse gas measurement systems based on cavity ring-down spectroscopy (CRDS) and a new drying system developed by KMA and the Korea Research Institute of Standards and Science (KRISS). This drying system is suitable in humid areas; water vapor measured using CRDS in ambient air was 0.001 % to 0.004 % across the stations. Measurement uncertainties expressed by the quadrature sum of the uncertainties from the drying system, scale propagations, repeatability, and reproducibility were ∼0.11 ppm from all KMA stations in the 68 % confidence interval. Average monthly CO2 enhancements above the local background at each station were 4.3±3.3 ppm at AMY, 1.7±1.3 ppm at JGS, and 1±1.9 ppm (1σ) at ULD, respectively, during 2012 to 2016. At AMY station, located between China and South Korea, CO2 annual means and seasonal variations are also greater than the other KMA stations, indicating that it is affected not only by local vegetation, but also added anthropogenic sources. Selected baseline CO2 at AMY and at JGS in the west of South Korea is more sensitive to East Asia (e.g., China) according to wind direction and speed. Through the comparison of long-term trends and growth rates at AMY with other East Asian stations over 15 years, it was suggested that they could be affected not only by local vegetation but also by measurement quality. </P>
Nonlinear Absortption processes in Triphenyamine-dithienothiophene-Triphyenylamine
Haeyoung Choi,Myoungsik Cha,han-Young Woo,Jong-Hyup Lee,kwanghee Lee,Kwang-sup Lee,Oh-Kil Kim 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.1
We investigated the nonlinear absorption mechanism in the triphenylamine-dithienothiophenetriphenylamine molecule by using laser pulses with two different pulse widths, 7 ns and 35 ps. The single-beam transmission measurement with a 7-ns pulse resulted in a larger nonlinear absorption than that with a 35-ps pulse and saturated at high input intensities due to the excited state absorption (ESA) initiated by two-photon absorption (TPA). For a quantitative characterization of the TPA and ESA contributions to the nonlinear absorption, we estimated the ESA spectrum and lifetime by using a pump-probe transmission measurement with a 35-ps pulse. A 5-level model was used to explain both the nonlinear absorption and the one-photon and the two-photon pumped fluorescence.
Near-infrared Transillumination and Photodynamic Therapy Using Hypericin in Animal Laryngeal Tumors
Lee Haeyoung,김성원,권다영,Kang Hyun Wook,Jung Min-Jung,Kim Jun Hyeong,Ahn Yeh-Chan,Oak Chulho 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.6
Background: We aimed to validate a pilot study of photodiagnosis using near infrared (NIR) transillumination and assess the clinical efficacy of hypericin-mediated photodynamic therapy (HYP-PDT) in a rabbit laryngeal cancer model in order to develop a novel therapeutic modality with complete remission and preservation of the functional organ. Methods: (1) In vitro study: VX tumor cells were subcultured and subjected to HYP-PDT. (2) In vivo study: A laryngeal cancer model was developed in which 12 rabbits were inoculated with a VX tumor suspension in the submucosal area of the left vocal fold using a transoral approach. All rabbits underwent NIR transillumination using light with a wavelength of 780 nm. The survival periods of the three treatment groups (6 rabbits in Group A: HYP-PDT, 3 each in Groups B and C: laser irradiation or HYP administration only) were analyzed. Results: The higher the HYP concentration, the lower the VX cell viability in response to HYP-PDT using 590 nm LED. Following HYP-PDT, small tumors in Group A-1 rabbits healed completely and the animals demonstrated a long survival period, and larger tumors in Group A-2 healed partially with a survival period that extended over 3 weeks after inoculation. The survival of Groups B and C were not different over the first 3 weeks of the study, and were shorter than in Group A. Conclusion: We found HYP-PDT could be a curative therapy for early-stage cancers that may also preserve organ function, and may inhibit tumor progression and metastasis during advanced stages of laryngeal cancer. Background: We aimed to validate a pilot study of photodiagnosis using near infrared (NIR) transillumination and assess the clinical efficacy of hypericin-mediated photodynamic therapy (HYP-PDT) in a rabbit laryngeal cancer model in order to develop a novel therapeutic modality with complete remission and preservation of the functional organ. Methods: (1) In vitro study: VX tumor cells were subcultured and subjected to HYP-PDT. (2) In vivo study: A laryngeal cancer model was developed in which 12 rabbits were inoculated with a VX tumor suspension in the submucosal area of the left vocal fold using a transoral approach. All rabbits underwent NIR transillumination using light with a wavelength of 780 nm. The survival periods of the three treatment groups (6 rabbits in Group A: HYP-PDT, 3 each in Groups B and C: laser irradiation or HYP administration only) were analyzed. Results: The higher the HYP concentration, the lower the VX cell viability in response to HYP-PDT using 590 nm LED. Following HYP-PDT, small tumors in Group A-1 rabbits healed completely and the animals demonstrated a long survival period, and larger tumors in Group A-2 healed partially with a survival period that extended over 3 weeks after inoculation. The survival of Groups B and C were not different over the first 3 weeks of the study, and were shorter than in Group A. Conclusion: We found HYP-PDT could be a curative therapy for early-stage cancers that may also preserve organ function, and may inhibit tumor progression and metastasis during advanced stages of laryngeal cancer.
Parameterization of 3D Surface Patches by Straightest Distances
Lee, Sungyeol,Lee, Haeyoung 홍익대학교 과학기술연구소 2006 科學技術硏究論文集 Vol.17 No.-
본 논문에서는 텍스처 맵핑(texture mapping), 모핑(morphing), 리메싱(remeshing), 기하학적 이미지(geometry image) 등의 토대가 되는 새로운 삼차원 표면의 형태 보존 선형 파라미터화(parameterization)를 소개한다. 3차원 표면을 2차원 평면으로 펼칠 때의 왜곡 현상을 줄이기 위해서 절단면을 이용한 국지적인 직선형 측지선을 이용하는 새로운 방법을 제안한다. 이 새롭고 간단한 방법을 기존의 대표적인 파라미터화 방법에 의한 결과와 비교하여 본다. 결론적으로, 앞으로 이 방법은 일대일 대응이 필요한 많은 응용 분야 도움을 줄 수 있을 것이다.