Liquid System을 이용한 적혈구계 세포 배양의 정량적 분석

한진영,김태겸,김경희,김아성,배혜란,서덕준,강설미,박주인,김인후 大韓血液學會 1997 大韓血液學會誌 Vol.32 No.1

정신질환으로 인한 총체적 질병부담에 대한 정신건강전문가들의 인식도 조사

김진영,정성훈,서동우,방수영,이해국,정인원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : The aim of this study was to investigate how mental health professionals in Korea evaluate the burden of disease caused by psychiatric disorders and to propose future directions of the public mental health policies. Methods : A questionnaire was mailed to 240 mental health professionals, who were composed of psychiatrists from mental hospitals and staffs who are nurses, clinical psychologists and social workers working in the community mental health centers in June, 2004. Two hundred twenty four out of two hundred forty subjects (93.3%), which were composed of 104 psychiatrists and 120 staffs, completed the questionnaire. The questionnaire included 6-item questions about the burden of disease in Korea. Results : Mental health professionals reported that the five diseases with the biggest burden were malignant neoplasms, traffic accidents, schizophrenia, alcohol use disorders and cerebrovascular diseases, consecutively. The burden of alcohol use disorders, cerebrovascular diseases, traffic accidents, unipolar depressive disorder and malignant neoplasms are expected to increase over the next several years. The proportions of psychiatric disorders among all the diseases in terms of burden of disease and medical costs were estimated as 18.4% (±12.8) and 12.0% (±11.7) respectively. Conclusion : This study showed that many of Korean mental health professionals have under-estimated burden of psychiatric disorders, especially, unipolar depressive disorder. It would be necessary to emphasize the importance of unipolar depressive disorder in applying educational and public programs for the mental health professionals, as well as for the general population.

Reference ranges for induced sputum eosinophil counts in Korean adult population

Kim, Mi-Yeong,Jo, Eun-Jung,Lee, Seung-Eun,Lee, Suh-Young,Song, Woo-Jung,Kim, Tae-Wan,Hur, Gyu-Young,Lee, Jae-Hyung,Kim, Tae-Bum,Park, Heung-Woo,Chang, Yoon-Seok,Park, Hae-Sim,Min, Kyung-Up,Cho, Sang-H Asia Pacific Association of Allergy, Asthma and Cl 2014 Asia Pacific allergy Vol.4 No.3

<P><B>Background</B></P><P>Induced sputum analyses are widely utilized to evaluate airway inflammation in asthmatics. However, the values have not been examined in Korean adults.</P><P><B>Objective</B></P><P>The purpose of this study is to determine reference ranges for induced sputum eosinophils and their influencing factors in Korean adults.</P><P><B>Methods</B></P><P>A total of 208 healthy nonasthmatic adults were recruited. Sputum induction and processing followed the international standard protocols.</P><P><B>Results</B></P><P>Adequate sputum samples were successfully collected from 81 subjects (38.9%). The upper 90 percentile for sputum eosinophil was calculated as 3.5%. The median value of eosinophil count percentage was significantly higher in subjects with atopy than those without atopy (median, 1.6%; range, 0-11.0% vs. median, 0%; range 0-3.6%, <I>p</I>=0.030). However, no significant correlations were found with age, gender, body mass index, smoking status, blood eosinophil, or fractional exhaled nitric oxide levels.</P><P><B>Conclusion</B></P><P>Current study was the first attempt to determine the reference ranges of induced sputum eosinophils in Korean adults. The cutoff value for sputum eosinophilia was 3.5%, and was significantly associated with atopy.</P>

견과류로부터 효율적인 DNA 추출 방법 비교

서승만(Seung-Man Suh),박샛별(Saet-Byul Park),김미주(Mi-Ju Kim),김해영(Hae-Yeong Kim) 한국식품과학회 2018 한국식품과학회지 Vol.50 No.4

본 연구에서는 견과류로부터 식품 분석에 사용될 DNA를 4가지 방법으로 추출하고 그 효율을 비교하였다. 동일한 양의 시료를 사용하여 추출된 DNA의 양은 CTAB법이 가장 우수한 것으로 확인되었지만, 추출 시간이 수배이상 오래 걸리고 유기용매를 사용한다는 한계점이 있다. 다른 방법들과 DNA 추출 양의 차이가 큰 잣, 캐슈너트, 피스타치오 너트, 땅콩의 시료는 CTAB법이 가장 효율적인 방법으로 판단되며, 호두, 헤이즐넛, 아몬드의 시료는 변형 CTAB법과 실리카 막법이 CTAB법을 대체할 수 있을 것으로 판단된다. 추출된 DNA를 식물 내재유전자 및 각 견과류에 특이적인 유전자를 사용하여 PCR을 진행하였으며, 모든 추출방법에서 DNA가 정상적으로 증폭되는 것을 확인하였다. This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.

3-(4`-Hydroxyl-3`,5`-dimethoxyphenyl)propionic Acid, an Active Principle of Kimchi, Inhibits Development of Atherosclerosis in Rabbits

( Hyun Ju Kim ),( Jin Su Lee ),( Hae Young Chung ),( Su Hee Song ),( Hong Suk Suh ),( Jung Sook Noh ),( Yeong Ok Song ) 부산대학교 김치연구소 2009 김치의 과학과 기술 Vol.12 No.-

The effects of 3-(4`-hydroxyl-3`,5`-dimethoxyphenyl)propionic acid (HDMPPA) originating from Korean cabbage kimchi were investigated, showing an antioxidant effect on the prevention of atherosclerosis in hypercholesterolemic rabbits. Twenty-one 3-month-old rabbits were fed an atherogenic diet containing 0.5% (w/w) cholesterol and 10% (w/w) coconut oil, whereas another two groups were given an atherogenic diet with intravenous injection of either HDMPPA or simvastatin (0.33 mg/kg/day) for 4 weeks. HDMPPA inhibited the oxidative modification of low-density lipoprotein (IC50 = 1.4 μg/mL) and increased 2,2`-diphenyl-1-picrylhydrazyl radical scavenging activity (IC50 = 0.78 μg/mL) in a dose-dependent manner. In hypercholesterolemic rabbits, the thickness of intima of aorta of the HDMPPA group was significantly reduced (control versus HDMPPA, 42%; simvastatin, 38%) without a plasma cholesterol-lowering effect. Thiobarbituric acid reactive substance formation in the plasma of the HDMPPA group was significantly decreased compared to that of the control group. Furthermore, the generation of vascular reactive oxygen species in HDMPPA group was suppressed as the cyclooxygenase-2 protein level decreased. These findings suggest that HDMPPA prevents the development of aortic atherosclerosis in high-cholesterol-fed rabbits. The antiatherosclerotic effect of HDMPPA may be due to an antioxidative effect at a low dose without cholesterol-lowering effects.

간암환자를 대상으로 한 래피드아크 치료계획에서 아크수 및 회전범위가 선량분포에 미치는 영향

박혜진,김미화,전미선,오영택,서태석,Park, Hae-Jin,Kim, Mi-Hwa,Chun, Mi-Son,Oh, Yeong-Teak,Suh, Tae-Suk 한국의학물리학회 2010 의학물리 Vol.21 No.2

본 논문에서는 간암 환자를 대상으로 3차원입체조형치료와 세기조절방사선치료와 3종류의 래피드아크 치료를 위한 치료계획을 수행하여 각각의 선량분포와 선량 체적 히스토그램(Dose Volume Histogram, DVH)의 특성을 비교, 평가하고 이를 통해 래피드아크 치료 환자에 대한 적절한 갠트리 회전수의 범위를 제시하고자 하였다. 치료계획은 작은 종양의 용적을 가지며 내부 장기 및 종양의 움직임이 비교적 작은 간암환자를 대상으로 3차원입체조형치료와 세기조절방사선치료와 더블아크(double arcs)와 제한적인 트리플아크(limited triple arcs)와 멀티플아크(multiple arcs)치료에 대해 Eclipse 8.6 버전에서 시행하였다. 또한 치료계획 시 임상표적용적(Clinical Target Volume, CTV)과 치료표적용적(Planning Target Volume, PTV)에 동일한 최적화 조건을 적용하였고, 각각의 정상조직에는 개별적인 선량 제한치 적용대신 종양 주변 정상조직에서의 선량 감소율을 일괄적으로 적용하였다. 임상표적용적과 치료표적용적의 전체 몸에 적용한 치료계획 선량 제한치에 대한 실제 계산결과의 만족도는 래피드아크가 세기조절방사선치료보다 높았으며 래피드아크에서 더블아크와 제한적인 트리플아크와 멀티플아크는 만족도가 거의 동일하였다. 또한 각 치료계획에서 치료표적용적에 계획한 선량 제한치에 따라 SALT group이 제안한 Conformity Index (CI)가 0.98인 선량 분포범위에 처방선량을 결정하였다. 이때 RTOG에서 제안한 CI, Homogeneity Index (HI), Quality of Coverage (QOC)와 Lomax and Scheib에서 제안한 Healthy tissue conformity index (HTCI) 등을 평가하였다. 항목별로 차이는 있지만 총체적인 평가 결과는 멀티플아크가 전체적으로 좋았으며 모니터 단위 값의 비교에서도 멀티플아크가 가장 작았다. 본 연구를 통하여 래피드아크 치료는 기존에 시행하고 있는 3차원입체조형치료 또는 세기조절방사선치료 기법보다 더욱 최적의 선량 분포를 구현한다는 것을 알 수 있었다. 또한 래피드아크 치료계획에서 멀티플아크 치료계획은 더블 또는 제한된 트리플아크 치료계획보다 모니터 단위 값이 작고, 선량의 균질성 및 종양내의 최소선량은 비슷하거나 좋아지는 동시에 주변 정상조직에는 선량이 감소되는 것을 알 수 있었다. In this paper, we evaluated the performance of 3D CRT, IMRT and three kind of RA plannings to investigate the clinical effect of RA with liver cancer case. The patient undergoing liver cancer of small volume and somewhat constant motion were selected. We performed 3D CRT, IMRT and RA plannings such as 2RA, limited triple arcs (3RA) and 3MRA with Eclipse version 8.6.15. The same dose volume objectives were defined for only CTV, PTV and body except heart, liver and partial body in IMRT and RA plannings. The steepness of dose gradient around tumor was determined by the Normal Tissue Objective function with the same parameters in place of respective definitions of dose volume objectives for the normal organs. The approach between the defined dose constraints and the practical DVH of CTV, PTV and Body was the best in 3MRA and the worst in IMRT. The DVHs were almost the same among RAs. Plans were evaluated using Conformity Index (CI), Homogeneity Index (HI) and Quality of coverage (QoC) by RTOG after prescription with dose level surrounding 98% of PTV in the respective plans. As a result, 3MRA planning showed the better favorable indices than that of the others and achieved the lowest MUs. In this study, RA planning is a technique that is possible to obtain the faster and better dose distribution than 3D CRT or IMRT techniques. Our result suggest that 3MRA planning is able to reduce the MUs further, keeping a similar or better targer dose homogeneity, conformity and sparing normal tissue than 2RA or 3RA.

벼담수 직파 재배에서 파종 입수가 생육 및 수량에 미치는 영향

이종철,문창식,서해영,최범열,Jong-Chul Lee,Chang-Sik Moon,Hae-Yeong Suh,Beom-Yeol Choi 한국작물학회 1973 한국작물학회지 Vol.- No.14

본 시험은 중부지방에서 담수직파재배에 알맞은 파종립수를 구명함과 파종립수에 EK른 수량 및 수량구성요소의 변이를 알고져 1972년에 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 최고분얼수는 밀파할수록 직선적으로 증가하여 280립 파종구에서 최고수치를 보였고 그 이후는 점차 감소되었으며 분얼에 대한 경합은 $m_2$당 280립이상 파종구에서 뚜렸했다. 2. 유효경비율은 $m_2$당 120립부터 280립 파종구까지는 파종밀도가 증가될수록 감소되다가 그 이후는 점차 증가되었다. 3. 최고분얼수가 $m_2$당 1,000개 이하에서는 얼자간 경합이 적었다. 4. 수수와 파종립수는 현저한정(+)의 상관이 인정되었고 수수와 일수영화수, 수수와 동숙비율은 (-)의 상관 경향이었다. 5. 수량과 $m_2$당 영화수는 r=0.929이며 수량과 수수는 r=0.695, 수수와 $m_2$당 영화수는 r=0.796이었다. 또한 수량과 수량구성요소간의 순위상관을 보면 수량과 수수가 가장 상관이 높았으며 (r=0.954_ 담수직파에서 수량을 지배하는 요인은 수수이었다. 6. 수량은 $m_2$당 280립 파종구에서 최고를 나타냈다. This study was carried out in the central part of Korea in 1972 to investigate optimum grain number per square meter and variation of the yield and its components in the direct sowing culture on irrigated paddy field. The results could be summarized as follows; 1. Number of maximum tillerings was directly increased with increase of grain number from 120 to 280 per square meter and it was gradually decreased with increase of number sowing grain. So number of maximum tillerings showed at the plot of 280 grain per square meter. Also it was showed remarkable competition at the plot more than 280 grain per square meter. 2. Percentage of effective tiller was decreased with increase of number of sowing grain from 120 to 280 per square meter and after it was gradually decreased with increase of number of sowing grain. 3. It was not remarkable competition in plot less than 1, 000 tillering per square meter on the number of maximum tillerings. 4. It was remarkable positive correlation between the number of panicle and the number of sowing grain, but it was tendency to negative correlation between the number of panicle and the number of spiklets per panicle, and between the number of panicle and the ripening ratio. 5. Correlation coefficient between the yield and the number of spiklet per square meter of land area was 0.929, r=0.695 in the yield and the number of panicle, r=0.796 in the number of panicles and the number of spiklets per square meter Also in rank correlation, it was showed most highly positive correlation(r=0.954) between the yield and the number of panicles, so number of panicles was most largely dominated on yield among the yield components in direct sowing culture on irrigated paddy field. 6. It was producted highest yield in the plot of 280 grain sowing per square meter.

Lactobacillus terrae sp. nov., a novel species isolated from soil samples in the Republic of Korea

Kim, Hyun-Joong,Lee, Hye Jin,Lim, Bora,Kim, Eiseul,Kim, Hae-Yeong,Suh, Minhwan,Hur, Moonsuk Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.9

$F_2$와 $F_5$ 상관에 의한 벼 Sink 및 Source 관련형질의 유전력 추정

하운구,김호영,최해춘,임상종,서학수,임무상,Ha, Woon-Goo,Kim, Ho-Yeong,Choi, Hae-Chune,Lim, Sang-Jong,Suh, Hak-Soo,Lim, Moo-Sang 한국작물학회 2002 Korean journal of crop science Vol.47 No.3

초다수성 품종 개발의 기초자료로 활용하고자 새로이 도입된 신초형 소얼 수중형 유전자원을 이용하여 F$_2$-F$_{5}$상관에 의한 sink-source관련 형질의 유전력을 추정한 결과를 요약하면 아래와 같다. 1. 일품벼에 YR15965 Acp 33이 교배된 F2와 F5세대간 상관정도에 의해 추정된 sink 및 source관련 형질들의 유전력은 specific leaf area, sink-source ratio와 harvest index의 0.009, 0.121, 0.013을 제외한 모든 형질에서 0.224이상의 높은 유전력을 보였다. 2. IR 66738-118-1-2에 가야벼가 교배된 후대에서는 모든 sink 및 source관련 형질이 0.001-0.286의 낮은 유전력을 보였다. 3. Sink 및 source관련 형질들과 수량과는 두 조합 모두 높은 싱관정도를 보였으나 등숙률과는 IR 66738-118-1-2에 가야벼가 교배된 후대에서는 대부분의 형질이 상관이 없었다. 그러나 일품벼에 YR 15965Acp33의 교배 후대에서는 등숙율과 source형질과는 높은 정상관이, sink capacity와 sink-source ratio등 sink형질과는 유의한 부의 상관이 있었다. The heritability of sink-source characters was estimated by regression coefficient between F$_2$ and F$_{5}$ in two crosses of IR 66738-118-1-2/Gayabyeo and the Ilpumbyeo/YR 15965 Acp 33. In order to get the some basic information for breeding of high yielding rice, genetic resources of new plant type with low tiller and heavy panicle were used. Most of the sink and source characters in Ilpumbyeo/YR 15965 Acp 33 cross showed high heritability over than 0.224. But the specific leaf area and sink-source ratio displayed low heritability being 0.009 and 0.013, respectively. Heritability of all sink and source characters in IR 66738-118-1-2/Gayabyeo cross ranged from 0.115 to 0.247. Correlation coefficient between yield and yield components in both combination were in the ranged between 0.001 and 0.247. But correlations among the yield components were not significant. Correlations between the yield and sink-source characters in both combinations were also highly significant. Particularly, correlation between the grain tiling ratio and most of the sink-source characters in IR 66738-118-1-2/Gayabyeo cross were not significant. In Ilpumbyeo/YR 15965 Acp 33 cross, correlations between grain filling ratio and source characters were positively significant. but sink characters (sink capacity and sink-source ratio, etc) were negatively significant.

The First Total Synthesis of 2,3,6-Tribromo-4,5-dihydroxybenzyl Methyl Ether (TDB) and Its Antioxidant Activity

Kim, Jae-Kon,Noh, Jung-Hyun,Lee, Sung-Eun,Choi, Jae-Sue,Suh, Hong-Suk,Chung, Hae-Young,Song, Yeong-Ok,Choi, Won-Chul Korean Chemical Society 2002 Bulletin of the Korean Chemical Society Vol.23 No.5