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Ha Ram Yang,Yeon Ju Park,Min Ji Kim,연제연,김완순 한국원예학회 2022 원예과학기술지 Vol.40 No.3
To check the availability of indoor plants, we investigated the growth and photo-physiological responses in Korean endemic Hosta minor for three months of artificial low light conditions (10, 30, and 50 μmol·m-2·s-1, abbreviated as L10, L30, and L50). In the L10 treatment, the growth indicators leaf number and size, shoot biomass, and tillering capacity declined by 20–50% decline from the initial values, and those were maintained or slightly increased in the L30 treatment and significantly increased to 200–300% in the L50 treatment. The light use efficiency values supported this result, which were –0.12, 0.04, and 0.16 in L10, L30, and L50, respectively. Growth adaptation to the indoor artificial lighting took four to six weeks. Net photosynthesis also decreased from the initial value after four weeks but increased in the ninth week, showing significant differences among the different light intensity levels. The light compensation point was 17 ± 6.16 μmol·m-2·s-1, and the light saturation point ranged from 200 to 350 μmol·m-2·s-1. Regardless of the light intensity, the Fv/Fm values were 0.79 to 0.83, indicating a normal level, and the OJIP curve also showed no significant difference. These results indicate that the low light intensity of 10 μmol·m-2·s-1 did not damage photosystem II but gradually negatively affected the growth of Hosta minor. We concluded that the minimum light intensity is 10 to 30 μmol·m-2·s-1 in Hosta minor and requires four weeks of low light adaptation. 한국 특산식물 좀비비추(Hosta minor)의 실내 이용을 위해 저광 조건(10, 30, 50µmol·m-2·s-1; L10, L30, L50)에서 3개월 간 생장 반응과 광 생리 특성을 조사하였다. 잎과 지상부, 분얼 생장 모두 초기생장(0주차)과 비교하여 L10에서는 증가 없이 오 히려 감소하였고(20–50%), L30에서는 유지되거나 약간 증가하였으며(10–50%), L50에서는 200–300% 수준으로 현저 하게 늘어났다. 처리기간 동안 건물 증가율은 L10, L30, L50에서 –20%, 10%, 90%, 광이용효율 역시 –0.12, 0.04, 0.16으로 나타났다. 순광합성량은 4주차 때는 초기값(0주차)보다 오히려 감소하였으나, 9주차 이후 증가하며 처리간 유의한 차이를 보 였다. 평균 광보상점은 17 ± 6.16µmol·m-2·s-1, 광포화점은 200–350µmol·m-2·s-1로 측정되었다. 광도와 관계없이 모든 처리 구 Fv/Fm 값은 0.79–0.83으로 정상 수준을 나타냈고, OJIP 곡선 역시 큰 차이를 보이지 않았다. 이상에서 10µmol·m-2·s-1 저 광도 조건은 광계Ⅱ 손상을 가져오지는 않았으나, 생장을 억제시키는 비생물적 스트레스 요인으로 작동하였다. 결론적으로 좀 비비추의 실내 이용을 위해서는 4주간의 적응이 필요하며, 한계 광도는 10–30µmol·m-2·s-1로 판단된다.
( So-jin Yang ),( Jin-a Ko ),( Hae-soo Kim ),( Min-ho Jo ),( Ha-nul Lee ),( Bo-ram Park ),( Young-min Kim ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a single protein band of 111 kDa. PDCITase showed optimum activity at pH 8.0 and retained 100% of activity within a broad pH range (7.0-11.5) after 18 h, indicating alkaliphilic or alkalistable CITase properties. In addition, PDCITase produced CI-7 to CI-17, CI-18, and CI-19, which are relatively large cycloisomaltooligosaccharides yet to be reported. Therefore, these large cycloisomaltooligosaccharides can be applied to the improvement of water solubility of pharmaceutical biomaterials.
Quantitative and Qualitative Estimation of Bacteria Contaminating Human Hairs
Yun, A-Ram,Yang, Eun-Jin,Lee, Young-Mi,Chae, Seon-Sook,Seo, Ha-Na,Park, Doo-Hyun The Korean Society for Microbiology 2010 Journal of Bacteriology and Virology Vol.40 No.1
Human hairs have been known to be easily contaminated with microorganisms. This study was performed in order to measure what bacterial species and how much microorganisms contaminate human hairs in specific place. Virgin human hairs were left at 6 positions in inside corner and beside window in a laboratory for 7 days. The number of viable bacterial cells, which were determined by most probable number method, contaminating the human hairs was measured at a maximum of $10^6/g$ hair and a minimum of $10^3/g$ hair in inside corner and maximum of $10^6/g$ hair and a minimum of $10^3/g$ hair beside window. The bacterial cells-contaminating human hairs were observed via fluorescence light microscopy after 4',6-diamino-2-phenylindole (DAPI) staining. The bacterial community contaminating human hairs was analyzed via the thermal gradient gel electrophoresis (TGGE) technique, based on the diversity of the 16S-rDNA variable region. In total, approximately 20 bacterial species were detected from 12 groups of hair samples. In this study, general experimental methods-fluorescence staining, TGGE and MPN-were combined to develop new method for observation and estimation of bacteria contaminating human hairs.
Recent Progress and Future Perspectives on Intestinal Organoids in Livestock
Inamul Haq,Hyeon Yang,Sun-A Ock,Ha-Yeon Wi,Kang-Won Park,Poong-Yeon Lee,Hyun-Woo Choi,Bo-Ram Lee 경상대학교 농업생명과학연구원 2021 농업생명과학연구 Vol.55 No.5
Three-dimensional (3D) organoids act as model systems because they mimic in vivo tissue morphology. Recent advancements in the field have demonstrated that organoids derived from various organs have assisted in understanding the underlying mechanisms of disease modeling and expanded our knowledge of organ development in vitro. Furthermore, these organoids have become a promising biomaterial in regenerative medicine for therapeutic purposes as well as in nutritional research for feed efficiency measurement in livestock. Intestinal organoids of livestock, including pigs, cattle, chickens, and horses, have been developed. These could be used to examine host-pathogen interactions, such as interaction between enteric viruses and epithelial cells, and are potential alternatives to in vivo systems. However, there are very limited studies regarding species-specific medium to cultivate and establish intestinal organoids of livestock. Species-specific medium is applied differently between species for the cultivation of intestinal organoids, and its modification is important for the maintenance of specific cell types or genes from the cellular diversity of the intestinal epithelium. In this study, we introduce the histological development of a 3D culture system and a species-specific medium for the cultivation of intestinal organoids in livestock. Finally, we discuss the importance and future perspectives of intestinal organoids in the fields of agriculture and biotechnology for various purposes.
The fifty-year history of dental hygiene education in South Korea
노희진,문소정,한선영,Ji-Hye Yang,Eun-Ha Jung,A-Ram Lee,Ju-Hui Jeong,정원균 한국치위생학회 2019 한국치위생학회지 Vol.19 No.5
The aim of this study was to review the history of dental hygiene education in Korea on its 50th anniversary in 2015. Internal resources and documents from the Korean Dental Hygienists’Association and other accessible resources were examined to verify the historical facts of dental hygiene education in Korea. From 1965 to 1967, the first class of four dental hygiene students graduated, and the legal basis for dental hygiene as a profession was established, which led to the start of the profession Registered Dental Hygienist in 1971. From 1977 to 1993, 13 higher educational institutions started dental hygiene programs. The Korean Dental Hygienists’ Association (1977) and Korean Association of Dental Hygiene College Professors (1987) were established in this period. From 1994 to 2006, the duration of major-intensive course programs in dental hygiene was extended to three from the two years of 1994, and the first bachelor's degree program in dental hygiene started in 2002. In this period, studies on dental hygiene became significantly active owing to vigorous activities by academic societies. The master's degree program in dental hygiene was established in 2007. Academic dental hygiene journals, the Journal of Dental Hygiene Science, and Journal of Korean Academy of Dental Hygiene Education were promoted as the registered journals of the Korean Research Foundation. From 1965 to 2015, dental hygiene education in South Korea expanded both quantitatively and qualitatively and is now ready for further progress in the future.
The fifty-year history of dental hygiene education in South Korea
Noh, Hie-Jin,Mun, So-Jung,Han, Sun-Young,Yang, Ji-Hye,Jung, Eun-Ha,Lee, A-Ram,Jeong, Ju-Hui,Chung, Won-Gyun Korean Society of Dental Hygiene 2019 한국치위생학회지 Vol.19 No.5
The aim of this study was to review the history of dental hygiene education in Korea on its $50^{th}$ anniversary in 2015. Internal resources and documents from the Korean Dental Hygienists' Association and other accessible resources were examined to verify the historical facts of dental hygiene education in Korea. From 1965 to 1967, the first class of four dental hygiene students graduated, and the legal basis for dental hygiene as a profession was established, which led to the start of the profession Registered Dental Hygienist in 1971. From 1977 to 1993, 13 higher educational institutions started dental hygiene programs. The Korean Dental Hygienists' Association (1977) and Korean Association of Dental Hygiene College Professors (1987) were established in this period. From 1994 to 2006, the duration of major-intensive course programs in dental hygiene was extended to three from the two years of 1994, and the first bachelor's degree program in dental hygiene started in 2002. In this period, studies on dental hygiene became significantly active owing to vigorous activities by academic societies. The master's degree program in dental hygiene was established in 2007. Academic dental hygiene journals, the Journal of Dental Hygiene Science, and Journal of Korean Academy of Dental Hygiene Education were promoted as the registered journals of the Korean Research Foundation. From 1965 to 2015, dental hygiene education in South Korea expanded both quantitatively and qualitatively and is now ready for further progress in the future.