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Ethylenediaminetetraacetic acid(EDTA)항응고제에 의한 Mean Corpuscular Volume(MCV)변화
홍승민,박영진,최하영,임진,정옥연,이호준,이호영,장숙진,문대수 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.2
It is essential that we put the anticoagulant into the blood specimen of the CBC test. However, the value of some parameters in the CBC test can be changed whenever using some anticoagulants. EDTA anticoagulant is mostly used these days. There are 3 kinds of EDTA, that is K_(2-), K_(3-), and Na_(2-) EDTA. The author studies how many effects each the EDTA gets into the true value of blood specimen for MCV of RBCs. Especially we compared the MCV effect of different concentrations of 3 EDTA anticoagulants on the bias with time and instrument. Some results are gotten. In our results, K_(3-)EDTA anticoagulant shows more satisfactory MCV value than K_(2) or Na_(2-)EDTA. On using concentration of EDTA, 1.5㎎ EDTA/㎖ of blood show more satisfactory MCV value than 7.5㎎ EDTA/㎖ of blood. However, the choice of anticoagulant may be dependant on the different instruments.
홍치유,조수열,백승도 동국대학교 자연과학연구소 1991 자연과학연구 논문집 Vol.11 No.-
X-ray 회절선을 조사한 결과 Ni?Cd?Fe?O?의 조성을 갖는 ltfy는 x값의 증가에 따라 inverse spinel 구조에서 normal spinel 구조로 변하며, 격자상수는 선형적으로 증가하였다. 또한 Mossbauer spectrum을 상온(300K)에서 측정하여 분석한 결과, 시료의 wrl적 성질은 x값의 증가에 따라 준강자성에서 상자성으로 변화되었으며, 시료는 Yafet-Kittel의 자기구조를 이루는 것을 알 수 있었다. According to the X-ray diffractometry, the structure of Ni?Cd?Fe?O? changes from inverse spinel to normal spinel and lattice constant varies linearly, as increasing x. From the result of the Mossbauer spectroscopic study, it is found, that the magnetic properties of Ni?Cd?Fe?O? change from ferrimagnetism to paramagnetism as increasing x, and the Ni?Cd?Fe?O? have the Yafet-Kittel magnetic structure.
가와사키병의 관상동맥 이상과 TLR2 유전자의 단일염기다형성
홍승수 외 중앙대학교 의과대학 의과학연구소 2009 中央醫大誌 Vol.34 No.3/4
Purpose: Many gene polymorphisms are associated with coronary artery abnormality in Kawasaki disease (KD). Toll like receptors (TLRs) are known to be involved in innate immunity. TLRs recognize structurally conserved molecules derived from microbes. Once these microbes have breached physical barriers such as the skin or intestinal tract mucosa, they are recognized by TLRs which activate immune responses. They activate nuclear factor-κB which produces diverse inflammatory cytokines. Polymorphism of TLR2 gene is reported to be associated with coronary artery abnormalities. The aim of this study was to evaluate the relationship between TLR2 polymorphism and coronary artery abnormality in Kawasaki disease. Methods: One hundred and one Korean children with Kawasaki disease and three hundred and six Korean healthy controls were enrolled in this study. The polymorphism of TLR2 gene was analyzed by direct sequencing. Results: There were no differences in the genotype (p =0.78) and allelic frequency (p =0.91) of the TLR2 rs3804099 and rs3804100 between KD and control subjects. There was no significant difference in TLR2 rs3804099 and rs3804100 polymorphism between patients with coronary artery lesion (CAL) and those without CAL. Conclusion: These results indicate that the polymorphisms of rs3804099 and rs3804100 in TLR2 gene might be not related in the development of coronary artery abnormalities in Kawasaki disease.
홍경한,전상신,이승재,박찬수,권일욱,김재열,김병철,하옥남 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.4
Fin-tube type(Fin-type) heat exchanger has been tested in order to replace the heat exchanger of parallel flow type(P.F-type) which is now widly used in automobile air conditioning system. The following conclusions are drawn by the comparison of the characteristics of the heat exchangers. Evaporator and condenser capacities and COP(Coefficience of performance) were varied as with the compressor speed, outdoor air temperature and air flow rate changed, which much influenced on the characteristics of the air conditioning system. Evaporator and condenser capacities were increased with increasing compressor speed and outdoor air temperature. Evaporator and condenser pressures of Fin- type were decreased by 7% and 5% respectively compared with those of P.F-type. The COP of Fin-type was decreased with increasing outdoor air temperature and compressor speed. The COP of P.F-type was decreased by 14% compared with that of Fin-type.
Hong‑Jai Lee,Bo‑Young Shin,Jae‑Seung Moon,Ailyn Fadriquela,Selikem Abla Nuwormegbe,Chun‑Chang Ho,Jin‑Su Shin,Jee‑Sang Yoon,Sang‑Kyou Lee,Soo‑Ki Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation in vitro and in vivo is unknown. Objective We explored whether BA, specifically taurochenodeoxycholic acid (tCDCA) would directly regulate the viral DNA and surface antigen expression of HBV in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. Result We found that higher concentration of taurochenodeoxycholic acid (tCDCA) is able to preserve the genomic stability of HBV in cell-free DMEM, showing higher the surface antigenicity than taurocholic acid (tCA). In line, we found that in vitro cell culture condition (100 μmol/L of tCDCA coupled with 1 × 108 g e/mL HBV) would be optimal for HBV entry into target cells. Using this, human (HepG2, Huh7), and rodent (Hepa1c1c7, H4-II-E) hepatoma cell lines were infected by HBV, as evidenced by the presence of HBV biomarkers (HBsAg, and HBV DNA in culture supernatant, as well as HBcAg in cell). Further, cellular entry test revealed that HBV is able to infect 12 different non-hepatic cell lines regardless of species, and organ/tissue, consequently reproducing progeny as confirmed by HBV biomarkers. Last, reinfection test showed that the progenies of HBV from immortalized HepG2, and Hepa1c1c7 cells are able to enter into each or vice versa naïve HepG2, and Hepa1c1c7 cells with or without BA. Conclusion This study demonstrates that enterohepatic humoral milieu such as BA, specifically tCDCA would directly regulate HBV DNA and its surface antigen expression in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. This is the first note to render HBV permissive to human or rodent hepatic and non-hepatic cells via sole manipulation of humoral milieu, thus establishing the platform for in vitro robust replication system of HBV.
홍성욱,김흥수 濟州大學校 産業技術硏究所 1995 산업기술연구소논문집 Vol.6 No.-
In the mobile radio communication system, we must study wave propagation characteristics for microcell and picocell environments because the conventional cellular enviornment is changing to microcell and picocell. In this paper, sample areas are selected to study propagation in variety of environment including the width of road, the built-up density and the height of building at Cheju city area. Sample area is separated two zones by break point at which the slope of the signal strength is changed. The location of the break point is calculated on first Fresnel zone clearance for antenna height variation. The slope and the standard deviation of the signal strength is obtained by the regression analysis. The break point distance can be used to define the size of microcell and to design of microcellular system.
홍성윤,김정민,김소영,이수정,오은실,양서인,김화실,김남초 병원간호사회 2006 임상간호연구 Vol.11 No.2
Purpose: The present study purposed to compare the hand washing effect of 7.5% powidone-iodine, which is used in the operation room of C university hospital in Seoul, with that of Ethyl alcohol-CHG mixture recommended by the US Association of Operating Room Nurses (2002) and to adopt a more effective hand disinfectant. Method: In a quasi-experimental design, 48 medical staff who participated in operations during the period from November 2004 to February 2005 had hand washing using the two kinds of hand disinfectants: 7.5% povidone-iodine and the ethyl alcohol-CHG mixture. Their palms were swabbed and cultured just after hand washing and again after taking dff sterile gloves after the operation. The number of colonies from the two occasions were counted and compared. Result: The number of general bacillus increased significantly in the group using 7.5% povidone-iodine compared to that in the group using the ethyl alcohol-CHG mixture. The number of general bacillus increased signficantly in doctors compared to that in nurses. The factors affecting the increase of the number of general bacillus were disinfectants and medical personnel. The number of general bacillus was expected to increase 9.41 times with 7.5% povidone-iodine than with the ethyl alcohol-CHG mixture and 14.87 times in doctors than in nurses. Conclusion: This study shows that the ethyl alcogol-CHG mixture has a stronger hand disinfection effect than 7.5% povidone iodine. Thus we need to change the hand disinfectant used in operating rooms as soon as possible in order to minimize the infection of wounds resulting from operations.