부산근해 유류분해세균의 생태학적 특성

남귀숙,이건,이상준 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

만성적인 유류 오염지역으로 간주되는 부산근해 10곳의 해수를 채취, 이화학적 특성과 이곳 해양 생태계내에 존재하는 종속영양세균수 및 유류분해 세균수, 그리고 그 분포비를 조사하였다. 해수내 유분의 농도와 일반 종속영양세균수에 대한 유류분해세균수의 생태계 내 분포비가 수영만 일대와 부산항 일대에서 높은 수치로 나타나 부산항 일대와 수영만 일대의 유류오염 정도가 타지역보다 높음을 알 수 있었으며, 오염된 유류가 해양생태계내에서 stress 인자로 작용, 유류분해 미생물의 군집내 증가현상을 나타내었다. 수차례의 농화배양을 거쳐 얻은 유류분해 세균 227개 균주중 분해능이 비교적 우수한 112개 균주만을 선정 동정한 결과, 환경정화자로서 주로 알려져 있는 종속영양세균인 Gram음성 간균의 Pseudomonas속, Enterobactericeae 관련속, Acinetobacter속 등의 11개 속과 Gram 양성 구균인 Micrococcus속 들로 나타났으며, 장내세균의 출현은 해양내 오수 유입을 반영하고 있었다. 배양적 특성, 생화확적 특성 및 생리적 특성 등 54개의 인자들을 이용하여 60% 유사도 수준으로 수리분류를 실시한 수 유류분해에 관련되는 미생물속 간의 유연관계를 조사한 결과 Pseudomonas속인 38개 세균들로 구성되어진 cluster 5 등 3개의 sp. 및 Pseudomonas sp.들로 구성된 cluster 2 그리고 Moraxella sp.로 구성된 cluster 5 등 3개의 Major cluster와 12개의 Minor cluster로 분석되었다. 종다양성 분석 결과, 정상적인 해양생태계 보다 부산항 일대 해양생태계에서의 종다양성 수치가 낮게 나타남으로써 유류유입이 생태계 안정성을 파괴함을 알 수 있었다. 또한, 부산근해 해양생태계내의 유류분해에 작용하는 세균중 Pseudomonas속이 우세한 생태학적 지위를 나타내었다. The bacteria capable of degrading and utilizing petroleum compounds as sole carbon and energy source were isolated from the seawater at 10 sites in pusan costal area as regarded the petroleum pollution zone, tested the physicochemical properties, and enumerated active heterotrophes and petroleum degraders. The results, examined for the distribution ratio of heterotrophes and petroleum degraders and the concentrations of petroleum in sea water, were high in Suyoung bay and Pusan harbor, do we could conclude that the area of there might be more polluted than others, and the petroleum polluted in marine ecosystem should act as stress factor which affected population. Taxonomical positions of 112 isolates were investigated. The results of identification were showed 11 genera of Gram nagative bacillus or cocobacillus which were Pseudomonas spp., Enterobacteriaceae, and Acinetobacter spp., etc., and 1 genus, Micrococcus spp., were Gram positive coccus. Apparence of Enterobacteriaceae indicated that the sea water was polluted with waste water. As a results of numerical taxonomy based on Jacchard coefficient, three major clusters and twelve minor clusters had been showed at the level of 60% similarity. Species diverisity analysis about petroleum degraders in the researched Pusan marine ecosystem had lower value than that in healthy one. Also genus of Pseudomonas had predominant ecological niche in petroleum degrading bacteria on Pusan costal area.

감잎가루를 첨가한 설기떡의 품질 특성에 관한 연구

김귀영,강우원,최상원 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.4

Sulgiduk is a typical Korean rice cake made by steaming rice powder with syrup and salt added. This study has measured moisture content, water activity, color, texture, and sensory evaluation of sulgiduk when persimmon leaves powder was added. The material used was sulgiduk to which the 0%(C), 1%(S₁) 2%(S₂), 3%(S₃), and 4%(S₄) of persimmon leaves powder were added. The moisture content of the raw rice powder for making sulgiduk was 32.40%, and that of the rice powder with syrup added was 37.97%~37,46%. The moisture content of the produced sulgiduk was 38.98%~37.93%, and the water activity was 0.97. In chromatic characteristics, the product had L value : of 73.86~59.16, a value: of -2.86~1.79 and b value: of 19.25~23.13. The texture characteristics such as hardness, fracturability, gumminess, cohesiveness chewiness and elasticity were not affected significantly by the persimmon leaves powder. In sensory evaluation, color, flavor, astringency, bitterness and overall quality were adversely affected with statistical significance (p<.001) by the addition of persimmon leaves. The juiciness and softness were also decreased as the persimmon leaves powder increased. In conclusion, it is recommended to add 1~2% of persimmon leaves powder to sulgiduk.

유전 알고리즘을 이용한 퍼지논리제어기 설계

裵相旭,朴太洪,朴貴泰 慶一大學校 1997 論文集 Vol.14 No.2

This paper presents a design method of fuzzy logic controller(FLC). In the proposed method, the parameters of FLC are determined by using Genetic Algorithms(GAs), which is an optimizing algorithm. Two types of FLC are considered. One is FLC with control rules whose fuzzy variables are all assigned by linguistic quantity. The other is FLC with control rules which are inferenced with simplified reasoning. For each FLC, the method of applying the GAs to determine the parameters of the FLC is proposed. By the simulation study for four systems with different characteristic, the usefulness of the proposed design scheme is discussed.

「飮食冊」의 調理에 관한 分析的 考察

김귀영,남궁석,이성우 東아시아食生活學會 1992 동아시아식생활학회지 Vol.2 No.2

Eum Sik Check is a cooking book written in old Korean by a woman called Dan Yang Daek. In this study, the contents of the book are interpreted in modern Korean, and are analyzed, compared, and examined in the point of view of cooking. The book shows 1) the methds of the setting of a large table, of a table for ancestor-memorial services, of decorative seasonings, 2) 11 methods for making rice cakes, 3) 21 methods for side dishes, and 4) 7 methods for brewing rice wine.

SOFC의 산소극 특성에 관한 연구

이성일,김귀열 충주대 산업과학기술연구소 1996 産業科學論文集 Vol.4 No.-

In this paper, the cathode materials of solid oxide fuel cell were fabricated, and the analysis of TG/DTA, XRD, thermal expansion were investigated. As a result, thermal expansion coefficient of LSM was not quite different from that of electrolyte. And a performance of LSM(x=0.4) was better excellent than the others.

급성 지주막하 출혈에 동반된 관상동맥 연축에 의한 심근경색증 1예

부귀범,박근호,양종태,이동민,장성종,김건영,장경식 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

In many cases, acute cerebrovascular disease is accompanied by abnormal electocardiograms. Also, though rather uncommon, acute myocardial infraction is also seen. The mechanism of its occurance is uncertain but it is thought to be related to coronary artery spasms, an abnormal autonomic nervous system, and a catecholamine increase. A female patient of 34 years of age came to the hospital because of sudden headache accompanied by substernal chest pain. A subarachnoid hemorrhage due to a cerebral aneurysm rupture had occured, and there was ST segment elevation in lead II, III, and aVF of the electrocardiogram. A cardiac enzyme test, an echocardiography, and a coronary angiography were performed, and she was diagnosed to have acute myocardial infarction due to coronary artery spasm. Clipping of the aneurysm was performed. With the use of nitrate and an angiotension converting enzyme inhibitor, the condition of the patient improved.

긴볼레기말의 항고지혈증 효과에 관한 연구 : Triton WR-1339 주사에 의한 고지혈증 유발 생쥐의 간세포내 지방 축적 감소를 중심으로 Based on the Decreasing Effect of Lipid Accumulation in Hepatocyte of Murine with Hyperlipidemia induced by Triton WR-1339

박인식,안상현,정재만,강윤호,이해풍,서귀문,홍용기,김호현,김진택 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 긴볼레기말 추출물의 항고지혈증 효과를 조사하기 위해 ICR 생쥐에 TritonWR-1339(TX) 복강주사로 인위적인 고지혈증을 유발시킨 후 긴볼레기말 추출물(30㎎/㎏)를 복강주사하여 시간의 경과에 따른 간세포내에서의 지방 축적 변화를 조직화학적으로 관찰하였다. TX 주사후 그물구조의 세포질출 가진 간세포가 간엽 전체에서 관찰되었고, 일부 간소엽에서는 간세포 손상으로 인한 간세포판 소실이 나타났다. 또한 간세포내 지방축척도 증가하여 전체 간소엽의 간세포에서 지방의 과출현을 확인 할 수 있었고, 지방의 크기도 대조군에 비해 증가된 것으로 관찰되었다. 그러나 긴볼레기말 추출물 주사군에서는 그물구조의 세포질을 가진 간세포의 수가 TX 주사군에 비해 감소되었고, 대부분의 간소엽에서 정상적인 간세포판의 배열을 확인할 수 있었다. 간세포내의 지방 축적과 크기도 감소된 경향으로 관찰되었다. 이상의 결과로 볼 때 해조류 긴볼레기말 추출물은 고지혈증이 유발된 생쥐 간세포 내에서의 과도한 지방축적을 감소시키는 항고지혈증 효과를 하는 것으로 사료된다. Hepatic tissues of ICR mouse were intraperitoneally injeced with Colpomenia bullosa(CB) Extract after Triton WR-1339(TX) injection were observed to investigate the antihyperlipermic effect of CB extract for hyperlipidemic hepatic tissue caused by destruction of lipid metabolism. The hepatic tissues were obtained at hour-24, 48, and 72 after TX injection with CB extract treatment. And then these specimen were fixed in 10% neutral buffer solution and were cryocut. The tissue stained by H&E for general morphology and sudan black B for lipid distribution. The increase of hepatocyte having rneshlike cytoplasm were shown in all hepatic lobules after TX injection and the hepatic plates were disappeared in the region of meshlike hepatocyte aggregation, But the hepatocyte having meshlike cytoplasm were disappeared and hapatic plate were rearranged in CB extract injected mouse. The number of blue black colored lipid drop in hepatic cytoplasm of mouse injected with TX were increased and the size of lipid drop were enlarged. But the number of lipid drop in hepatic cytoplasm of mouse treated CB extract were decreased and the size of lipid drop were diminished. As results indicated that the accumulation of lipid drop caused by TX injection were mitigated by the antihyperlipidermic effect of CB extract.

위장관 출혈을 일으킨 공장의 형활근종 1예

김병수,장성종,부귀범,조운택,박찬국,김만우,변주남,김정용,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Small bowel leiomyoma is a rare tumor which may occur wherever smooth muscle is present. The small bowel is the most common site and it makes up 20~30% of all benign small intestinal neoplasms. A spectrum of symptoms are common ranging from nausea, vomiting, abdominal pain, to massive intestinal bleeding. Bleeding is the most common symptom and is usually gross and recurrent. Occasionally it may be life threatening. But, the most serious problem is both the absence of specific clinical symptoms, and the difficulties shown in radiological diagnostics of the small bowel. If conventional diagnostic methods fail to reveal any positive findings for gastrointestinal bleeding, the diagnosis of intestinal leiomyoma should be suspected and an SMA angiography shoud be performed. The angiographic criteria of small bowel leiomyoma is very characteristic. We present a case of leiomyoma with considerable and prolonged gastrointestinal bleeding, which was diagnosed by angiography. A 61-year old woman was admitted with a history of melena over the last 10 days. An SMA angiography showed a hypervascular mass with a diameter of 3×3cm. A segmental resection was performed under the impression of the gastrointestinal bleeding due to leiomyoma of the small bowel. Microscopic examination of the lesion shows interlacing or whorl bundles of spindle shaped ces and no mitosis.

자동차용 충격 흡수기의 동특성 연구

남경탁,황성원,신귀수 한국산업안전학회 2002 한국안전학회지 Vol.17 No.3

A dynamic characteristics of shock absorber in the various excitation is investigated experimentally. Work diagrams and characteristic curves are used as a experimental standard. The various excitation conditions, temperature and noise are very important factors in associated with the reduction of damping force. It is found that the heat occurrence from shock absorber, the gas shock absorber is much than oil shock absorber and increased in high speed. As to the variation of damping force, there are no change when the speed is low, but we fixed amount of variation by increasing speed and change of new and old decrease. The sound pressure of the swash noise from cycle of shock absorber, we compared with theory sound pressure by experiment.

황해 직선 황로상 대기오염물질의 측정

이승복,배귀남,진현철,김영성,문길주,심상규 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.1

Air pollutants were measured eight times from June 1999 to June 2002 on regular ferries cruising across the Yellow Sea between Incheon in Korea and Qingdao or Tianjin in China. PM_(10) and PM_(2.5_ were measured as particulate matters and SO₂, CO, and NOx were measured as gaseous pollutants. On each route, sampling was made, starting two hour after departure and ending two hour before arrival. Low concentrations of gaseous pollutants that were not much varied according to sampling period and location revealed that atmosphere over the sea was not directly affected by anthropogenic emissions. However, concentrations of fine particles were generally higher than those measured at Deokjeok Island. 50 km west of the western seashore, at similar periods. It was believed that considerable influence of China in the form of secondary pollutants was exerted over the sea.