Invasiveness evaluation of fireweed (Crassocephalum crepidioides) based on its seed germination features

Guo Qi Chen,Shui Liang Guo,Qiu Sheng Huang 한국잡초학회 2009 Weed Biology and Management Vol.9 No.2

Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.

Identification and Characterization of an Antifungal Protein, AfAFPR9, Produced by Marine-Derived Aspergillus fumigatus R9

( Qi Rao ),( Wen Bin Guo ),( Xin Hua Chen ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5

A fungal strain, R9, was isolated from the South Atlantic sediment sample and identified as Aspergillus fumigatus. An antifungal protein, AfAFPR9, was purified from the culture supernatant of Aspergillus fumigatus R9. AfAFPR9 was identified to be restrictocin, which is a member of the ribosome-inactivating proteins (RIPs), by MALDI-TOF-TOF-MS. AfAFPR9 displayed antifungal activity against plant pathogenic Fusarium oxysporum, Alternaria longipes, Colletotrichum gloeosporioides, Paecilomyces variotii, and Trichoderma viride at minimum inhibitory concentrations of 0.6, 0.6, 1.2, 1.2, and 2.4 μg/disc, respectively. Moreover, AfAFPR9 exhibited a certain extent of thermostability, and metal ion and denaturant tolerance. The iodoacetamide assay showed that the disulfide bridge in AfAFPR9 was indispensable for its antifungal action. The cDNA encoding for AfAFPR9 was cloned from A. fumigatus R9 by RTPCR and heterologously expressed in E. coli. The recombinant AfAFPR9 protein exhibited obvious antifungal activity against C. gloeosporioides, T. viride, and A. longipes. These results reveal the antifungal properties of a RIP member (AfAFPR9) from marine-derived Aspergillus fumigatus and indicated its potential application in controlling plant pathogenic fungi.

Estimation Algorithm of Minimum Dwell Time in Precision Cylindrical Plunge Grinding Using Acoustic Emission Signal

Chen Jiang,Qi Song,Debao Guo,Haolin Li 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

This work presents an investigation of the minimum dwell time during the precision cylindrical-plunge grinding process. Accordingto the model of acoustic emissions proposed in earlier work, two estimation algorithms of the dwell time, focused on the size errorand the surface roundness of the machined workpiece, are deduced from the models of the material removal and the surfaceroundness, respectively. Based on the proposed estimation algorithms, an on-line estimation method of the minimum dwell time isdeveloped using the measurement and analysis of the acoustic emission signal. A series of experiments of grinding C45 steel areconducted to confirm the validity of the proposed estimation algorithms and determine the model coefficient. The ability of theestimation algorithms to predict the minimum dwell time is deemed necessary for optimal control of the grinding process. In addition,the proposed model provides a new approach to estimate the machined qualities of a workpiece such as the surface roughness andsize error using the dwell time of the grinding process.

Diversity of Pleurotus nebrodensis strains and detection a specific molecule marker

Qi Tan,C. Peter Romaine,Carl Schlagnhaufer,Mingjie Chen,Qian Guo,Jozsef Geml 한국버섯학회 2004 한국버섯학회 국제학술대회 Vol.3 No.-

Effect of humidity on mechanical properties of dissolving microneedles for transdermal drug delivery

Qi Lei Wang,Jia Wei Ren,Bo Zhi Chen,Xuan Jin,Can Yang Zhang,Xin Dong Guo 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-

Dissolving microneedles (DMNs) prepared from soluble polymers has been being widely investigated as a safe and effective transdermal delivery system for drug and vaccine. Limited by the materials, the air humidity would probably affect the mechanical property of DMNs, which is essential for the effective needle insertions and drug delivery. In this study, four kinds DMNs prepared by polyvinyl alcohol, sodium hyaluronate (HA), chitosan and gelatin are studied on the mechanical strength and insertion ability under varying humidity conditions. Based on these investigations, we hope to provide the effective time of DMNs to ensure a successful insertion and drug delivery.

Fatigue performance and life prediction methods research on steel tube-welded hollow spherical joint

Qi Guo,Ying Xing,Honggang Lei,Jingfeng Jiao,Qingwei Chen 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.36 No.1

The grid structures with welded hollow spherical joint (WHSJ) have gained increasing popularity for use in industrial buildings with suspended cranes, and usually welded with steel tube (ST). The fatigue performance of steel tube-welded hollow spherical joint (ST-WHSJ) is however not yet well characterized, and there is little research on fatigue life prediction methods of ST-WHSJ. In this study, based on previous fatigue tests, three series of specimen fatigue data with different design parameters and stress ratios were compared, and two fatigue failure modes were revealed: failure at the weld toe of the ST and the WHSJ respectively. Then, S–N curves of nominal stress were uniformed. Furthermore, a finite element model (FEM) was validated by static test, and was introduced to assess fatigue behavior with the hot spot stress method (HSSM) and the effective notch stress method (ENSM). Both methods could provide conservative predictions, and these two methods had similar results. However, ENSM, especially when using von Mises stress, had a better fit for the series with a non- positive stress ratio. After including the welding residual stress and mean stress, analyses with the local stress method (LSM) and the critical distance method (CDM, including point method and line method) were carried out. It could be seen that the point method of CDM led to more accurate predictions than LSM, and was recommended for series with positive stress ratios.

Migraine Susceptibility Genes in Han Chinese of Fujian Province

Qi-fang Lin,Zi-chun Chen,Xian-guo Fu,Jing Yang,Luo-yuan Cao,Long-teng Yao,Yong-tong Xin,Gen-bin Huang 대한신경과학회 2017 Journal of Clinical Neurology Vol.13 No.1

Background and Purpose Five single-nucleotide polymorphisms (SNPs) (rs4379368, rs10504861, rs10915437, rs12134493 and rs13208321) were recently identified in a Western population with migraine. These migraine-associated SNPs have not been evaluated in a Han Chinese population. This study investigated the associations of specific SNPs with migraine in a Han population. Methods This was a case-control study of Han Chinese residing in Fujian Province. Polymerase chain reaction—restriction-fragment-length polymorphism analysis and direct sequencing were used to characterize the relationships of SNPs in a control group of 200 subjects and in a migraine group of 201 patients. Results The frequencies of the five SNPs did not differ between patients with migraine and healthy non migraine controls. However, subgroup analysis indicated certain SNPs were more strongly associated with migraine with aura or migraine without aura than with controls. The CT genotype of rs4379368 was more common in migraine patients with aura (75%) than in migraine patients without aura (47.9%) and controls (48.5%) (p<0.05), and the TT genotype of rs10504861 was more common in migraine patients with aura than in controls (8.3% vs. 0.5%) (p<0.05). Meanwhile, the CC genotype of rs12134493 was less common in migraine patients without aura than in controls (80.6% vs. 88%) (p<0.05). Conclusions Our findings suggest that the rs4379368 and rs10504861 SNPs are markers for susceptibility to migraine with aura and that rs12134493 is a marker for the risk of migraine without aura in this Han population. Future studies should further explore if these associations vary by ethnicity.

Electrospun Polyphenylquinoxaline Ultrafine Non-woven Fibrous Membranes with Excellent Thermal and Alkaline Resistance: Preparation and Characterization

Chen-yu Guo,Lu-meng Yin,Jin-gang Liu,Xin-ke Wang,Na Zhang,Lin Qi,Yan Zhang,Xiao Wu,Xiu-min Zhang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.12

A series of polyphenylquinoxaline (PPQ) ultrafine non-woven fibrous membranes have been first successfullyprepared via the electrospinning procedure with the soluble PPQ solutions as the starting materials. For this purpose, variousorgano-soluble PPQ resins were synthesized via the one-step high temperature polycondensation procedure from thearomatic ether-bridged bis(α-diketone) and bis(o-diamine) monomers. Flexible ether linkages and pendant bulky phenylsubstituents endowed the PPQ resins good solubility in polar aprotic solvents. The high-molecular-weight PPQ resins weredissolved in N-methyl-2-pyrrolidone (NMP) to afford the PPQ electrospinning solution except PPQ-Ia derived from 4,4'-oxydibenzil (ODB) and 3,3'-diaminobenzidine (DAB) due to the limited solubility in the solvent. All the derived PPQultrafine non-woven fibrous membranes maintained good structure integrity after hydrolysis aging either at room temperature(25 oC) for 72 h or at refluxing temperature (100 oC) for 24 h in an aqueous sodium hydroxide (NaOH) solution at a solidcontent of 20 wt%. Comparatively, the polyimide (PI) reference electrospun membrane (PI-ref) derived from 1,2,4,5-pyrromellitic anhydride (PMDA) and 4,4'-oxydianiline (ODA) lost its original structure only after boiling in the same NaOHsolution within 3 h. In addition, the developed PPQ ultrafine non-woven fibrous membranes exhibited good thermal stabilitywith the 5 % weight loss temperatures (T5%) higher than 555.0 oC in nitrogen and glass transition temperatures (Tg) in therange of 248.1-266.1 oC, respectively.

Involvement of MicroRNA-198 Overexpression in the Poor Prognosis of Esophageal Cancer

Qi, Bo,Yao, Wen-Jian,Zhao, Bao-Sheng,Qin, Xiu-Guang,Wang, Yi,Wang, Wen-Ju,Wang, Tian-Yun,Liu, Shang-Guo,Li, Han-Chen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: This study aimed to investigate whether the miR-198 expression level is related to clinicopathological factors and prognosis of esophageal cancer. Methods: MicroRNA was extracted from esophageal cancer patients who underwent surgery for assessment using the Taqman@ MicroRNA assay. The correlation between miR-198 expression and clinicopathological features was analyzed, and the significance of miR-198 as a prognostic factor and its relationship with survival was determined. Results: MicroRNA-198 (miR-198) expression was higher in patients with poor prognosis than those with good prognosis (P<0.05). Kaplan-Meier analysis results showed that the miR-198 expression level had a significant correlation with survival time (P=0.030) and that patients with a higher expression of miR-198 had a shorter survival time. Cox multi-factor model analysis showed that patient prognosis (P=0.014), tumor length (P=0.040) and expression (P=0.012), and survival time had a significant correlation; the corresponding risks were 7.268, 1.246, and 3.524, respectively. Conclusion: miR-198 overexpression is involved in the poor prognosis of esophageal cancer and can be used as a biomarker for selection of cases requiring especial attention.