Early Recognition of Pediatric Strokes in the Emergency Department: Epidemiology, Clinical Presentation, and Factors Impeding Stroke Diagnosis in Children

Si Qi Tan(Si Qi Tan),Wen Qi Cher(Wen Qi Cher),Shu-Ling Chong(Shu-Ling Chong),Angelina Su Yin Ang(Angelina Su Yin Ang ),Sashikumar Ganapathy(Sashikumar Ganapathy ),Derrick Wei Shih Chan(Derrick Wei Shi 대한소아신경학회 2022 대한소아신경학회지 Vol.31 No.1

Purpose: Strokes are challenging to diagnose in pediatric emergency departments (EDs) as level of suspicion is low and atypical presentations are common. We analyzed clinical features, epidemiology and factors of delayed identification in arterial ischemic strokes (AIS) and hemorrhagic strokes (HS). Methods: Single-center retrospective cohort study of children aged between 29 days and 18 years old diagnosed with stroke between July 2016 to June 2021. Results: Among 36 children, 11 (30.5%) had AIS, 25 (69.4%) had HS. Median age for AIS was 9 years (interquartile range [IQR], 2 to 9) and HS 9 years (IQR, 1 to 11.5) (P=0.715). Focal neurological deficit was seen in 72.7% of AIS and 20% of HS (P=0.006). Only 18.2% of AIS and 52.0% of HS presented within 6 hours of symptoms. Median time from symptom onset to ED presentation was 24 hours (IQR, 12 to 28) for AIS and 7 hours (IQR, 1.8 to 48) for HS (P=0.595). Most (85.6%) arrived by own transport. Median time from presentation to neuroimaging was 7 hours (IQR, 0.9 to 7) for AIS and 4.8 (IQR, 1.3 to 16.8) hours for HS (P=0.376). Eleven patients, 9/25 (36.0%) HS and 2/11 (18.2%) AIS, did not have stroke as differential diagnosis at ED (P=0.714). Common initial diagnoses were viral illness or headaches. On univariate analysis, age <1 (odds ratio [OR], 17.5; 95% confidence interval [CI], 1.2 to 250.4; P=0.035) and absence of focal neurological deficit (OR, 13.091; 95% CI, 1.5 to 117.9; P=0.022) were significant factors for delayed identification. Conclusion: Index of suspicion for pediatric strokes among caregivers and clinicians should be increased. Public awareness campaigns are recommended.

Diversity of Pleurotus nebrodensis strains and detection a specific molecule marker

Qi Tan,C. Peter Romaine,Carl Schlagnhaufer,Mingjie Chen,Qian Guo,Jozsef Geml 한국버섯학회 2004 한국버섯학회 국제학술대회 Vol.3 No.-

Research and development of edible fungi industry in modern china

Tan Qi 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Related research on artificial cultivation of the edible fungi in modern China can be traced back to 100 years ago. The earliest article about the cultivation techniques of the edible fungi was published in 1897 on Agricultural Study Newspaper sponsored by Shanghai Agricultural Society. From the late 1800s to 1940s, the elder generation of scholars including Zou Bingwen, Hu Changzhi, Pan Zhinong, Li Shiyi, Sun Yunwei, and Yu Xiaotie, etc. not only introduced many foreign techniques in edible fungi cultivation a nd disseminated scientific knowledge and cultivation techniques, but also held various edible fungi talents training courses, set up experimental bases, and conduct edible fungi cultivation experiments. Those have laid a preliminary foundation for the modernization of China’s edible fungi cultivation techniques. After the founding of the PRC, the edible fungi cultivation industry has gained more space for development, and has achieved many milestone achievements, mainly including the tremella artificial cultivation technique, the hedgehog hydnum artificial cultivation technique, the Xianggu artificial cultivation with crushed-wood material technique, the white mushroom fine breed selection and breeding and cultivation technique improvement, the black fungus cultivation with crushed-wood material and fine breed selection and breeding, and the golden needle mushroom find breed selection and year-round cultivation technique innovation, as well as the acclimatization of wild edible fungi and the development of new varieties of edible fungi. These inventions and innovations have provided solid technical support to the development of the edible fungi industry in China. The reform and opening-up starting 1978 has provided a favorable policy environment for the development of the edible fungi industry in China. In the period of more than 30 years thereafter, the edible fungi industry in China has been developing rapidly, with the annual yield rocketing from 60,000 tons in 1978 to 20.2 million tons in 2009, and the proportion of the yield against total world yield growing from 5% in the past to more than 70% at present. During this historical period, many research institutions and scientific research staffs have made important contributions to the development of the edible fungi industry in China. Among them, the most important achievements are made by Researcher Chen Meipeng from the Institute of Edible Fungi of Shanghai Academy of Agricultural Sciences, Professor Yang Xinmei from Huazhong Agricultural University, Researcher Huang Nianlai from Fujian Sanming Mycological Institute, and Professor Zhang Shuting from Chinese University of Hong Kong. When we look back, we treasure the outstanding achievements made by the elder generation of scholars on the development of the edible fungi industry in China. When we look into the future, we are geared with enthusiasm and confidence, and we will work hard to achieve higher in the edible fungi industry in China.

Protective effect of procyanidin B2 on hydrogen peroxide (H2O2)-induced oxidative damage in MCF-7 cells

Tan Jia-qi,Li Peng-cheng,Li Qian,Tang Jin-tian,Xue Hong-kun 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.5

The aim of this study is to assess the cytoprotection and potential molecular mechanisms of procyanidin B2 ( PCB2) on hydrogen peroxide ( H2O2)-induced oxidative damage in MCF-7 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the viability of MCF-7 cell exposure to H2O2 or PCB2. We measured the antioxidant properties of PCB2 by determining the activities of SOD, GSH-Px, LDH and MDA levels, and evaluated apoptosis and intracellular reactive oxygen species (ROS) levels. The related proteins expression levels were monitored by Western blot. MCF-7 cells induced with H2O2 had a remarkable decrease in cell viability that was suppressed when it was interfered with PCB2 (0.1–10.0 μM). PCB2 interference memorably and dose-dependently inhibited H2O2- induced LDH leakage, ROS and MDA overproduction, while PCB2 markedly increased H2O2- induced the activities of SOD and GSH-Px. Eventually, H2O2 prominently down-regulated the ratio of Bcl-2/Bax and the relative proteins expression levels of Nrf2, GCLC, NQO1 and HO-1, and up-regulated the relative proteins expression levels of cytochrome c, caspase-3 and Keap1. However, the relative expression levels of these proteins were reversed in PCB2- interfered MCF-7 cells. This study implied that protective effect of PCB2 on H2O2- induced oxidative damage in MCF-7 cells might be related to inhibition of mitochondria-dependent apoptosis, activation of Keap1/Nrf2/HO-1 signaling pathway and improvement of the antioxidant enzymes activities.

Research and development of edible fungi industry in modern china

Tan Qi 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-

Pyoderma Gangrenosum in a Patient with X-Linked Agammaglobulinemia

( Qi Tan Fa-liang Ren ),( Hua Wang ) 대한피부과학회 2017 Annals of Dermatology Vol.29 No.4

X-linked agammaglobulinemia (XLA) is a primary im-munodeficiency disorder caused by germline mutations of B-cell tyrosine kinase (BTK) gene. It is characterized by de-creased serum immunoglobulins levels and circulating ma-ture B cells. This defect in humoral immunity leads to in-creased susceptibility to infection. Pyoderma gangrenosum (PG) is an uncommon, ulcerating, neutrophilic dermatosis. Here we report PG in an 8-year-old patient with XLA. The pa-tient received intravenous immunoglobulin treatment in conjunction with prednisone and topical application of 0.03% tacrolimus ointment and the ulcer was almost com-pletely healed in the 2 weeks of follow-up. The coexistence has been rarely reported. XLA may be a possible cofactor in the pathogenesis of PG. (Ann Dermatol 29(4) 476∼478, 2017)

Higher-order assumed stress quadrilateral element for the Mindlin plate bending problem

Tan Li,Zhaohui Qi,Xu Ma,Wanji Chen 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.3

In this paper an 8-node quadrilateral assumed stress hybrid Mindlin plate element with 39 is presented. The formulation is based on complementary energy principle. The proposed element is free of shear locking and is capable of passing all the patch tests, especially the non-zero constant shear enhanced patch test. To accomplish this purpose, special attention is devoted to selecting boundary displacement interpolation and stress approximation in domain. The arbitrary order Timoshenko beam function is successfully used to derive the boundary displacement interpolation. According to the equilibrium equations, an appropriate stress approximation is rationally derived. Particularly, in order to improve element’s accuracy, the assumed stress field is derived by employing 39 rather than conventional 21. The resulting element can be adopted to analyze both moderately thick and thin plates, and the convergence for the very thin case can be ensured theoretically. Excellent element performance is demonstrated by a wide of experimental evaluations.

Radiation-induced Cochlea Hair Cell Death: Mechanisms and Protection

Tan, Pei-Xin,Du, Sha-Sha,Ren, Chen,Yao, Qi-Wei,Yuan, Ya-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Cochlea hair cell death is regarded to be responsible for the radiation-induced sensorineural hearing loss (SNHL), which is one of the principal complications of radiotherapy (RT) for head and neck cancers. In this mini-review, we focus on the current progresses trying to unravel mechanisms of radiation-induced hair cell death and find out possible protection. P53, reactive oxygen species (ROS) and c-Jun N-terminal kinase (JNK) pathways have been proposed as pivotal in the processes leading to radiation hair cell death. Potential protectants, such as amifostine, N-acetylcysteine (NAC) and epicatechin (EC), are claimed to be effective at reducing radiation-inducedhair cell death. The RT dosage, selection and application of concurrent chemotherapy should be pre-examined in order to minimize the damage to cochlea hair cells.

Inhibition of ERK and Activation of p38 are Involved in Diallyl Disulfide Induced Apoptosis of Leukemia HL-60 Cells

Tan, Hui,Ling, Hui,He, Jie,Yi, Lan,Zhou, Jianguo,Lin, Min,Su, Qi 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

We investigated the effects of diallyl disulfide (DADS) on the induction of apoptosis in human Leukemia cell line HL-60 and explored the roles of mitogen-activated protein kinase (ERK and p38 MAPK) pathways in the growth inhibition and apoptosis induced by DADS. MTT assay was used to determine the DADS induced cell growth inhibition in HL-60 cells. Flow cytometry and DNA fragmentation were used to examine the roles of apoptosis in DADS-mediated cell death. Western blot analysis of the expression of phospho-MAPKs (ERK and p38) was employed to elucidate the possible mechanisms of DADS induced apoptosis. We found that growth inhibition of HL-60 cells treated with DADS exhibited a dose-dependent response (P<0.05) and DADS induced significant apoptosis. DADS at the concentration of 10 mg/L persistently activated p38 and simultaneously reduced ERK activity. PD98059, an inhibitor of ERK upstream activators MAPK kinase MKK1 and MKK2, promoted cytotoxicity and apoptosis in HL-60 cells treated with DADS. In contrast, SB203580, an inhibitor of p38, decreased cytotoxicity and apoptosis induced by DADS. Therefore, DADS can effectively inhibit the proliferation and induce apoptosis of human leukemia cell line HL-60. Inhibition of ERK signaling pathways and activation of p38 signaling pathways are likely involved in DADS induced apoptosis in HL-60 cells.

An Optimization Model for Low Carbon Oriented Modular Product Platform Planning (MP3)

Qi Wang,Dunbing Tang,Leilei Yin,Inayat Ullah,Libin Tan,Tao Zhang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.5 No.1

Consumers, industry, and government entities put more focus on the global warming. With this point in view, enterprises have started to take into account of the low carbon idea to develop products. Recently, lots of firms are using the Modular Product Platform Planning (MP3) between product generations for the rapid update of products with lesser production costs and time to market. Traditionally, MP3 mainly concerns on module sharing between product generations in modular planning, but pays no attention to the environmental concern which becomes an important design criterion due to the rising awareness of environmental protection. In this paper, an optimization model for low carbon oriented MP3 is presented. To support low carbon oriented MP3, this article proposed a probability approach to evaluate the scheme of modular planning for MP3. Meanwhile, a measurement method for low carbon performance of modular planning is provided. Then, an optimization model for low carbon oriented MP3 is formulated to determine the modular planning in pursuit of maximizing the low carbon performance of the product with controlling the implementation probability of MP3. Furthermore, an Adaptive Memetic Algorithm (AMA) is developed for solving the optimization problem. Finally, a case study is proposed to validate the effectiveness of the proposed approach.