Scutellaria Extract Decreases the Proportion of Side Population Cells in a Myeloma Cell Line by Down-regulating the Expression of ABCG2 Protein

Lin, Mei-Gui,Liu, Li-Ping,Li, Chen-Yin,Zhang, Meng,Chen, Yuling,Qin, Jian,Gu, Yue-Yu,Li, Zhi,Wu, Xin-Lin,Mo, Sui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background and Aims: Scutellaria is one of the most popular traditional Chinese herbal remedies against various human diseases, including cancer. In this study, we examined the active effects of Scutellaria extract and its main flavonoid constituents on the proportion of side population cells within human multiple myeloma cell line RPMI8226 in vitro and explored the potential molecular mechanisms involved. Materials and Methods: The contents of flavonoids in ethanolic extract of Scutellaria baicalensis Georgi were determined using high performance liquid chromatography. The antiproliferative effect of the ethanolic extract on RPMI-8226 was determined by CCK assay. Apoptosis was measured by annexin combining with propidium iodide in a flow cytometer. Cell cycle analysis was performed by propidium iodide staining in combination with flow cytometry analysis. Hoechst 33342 exclusion assay was used for the identification of side population within RPMI8226 cells. The expression of ABCG2 protein was assessed by Western blotting assay. Results: The content of major flavonoids constitutents of Scutellaria extract was baicalin (10.2%), wogonoside (2.50%), baicalein (2.29%), and wogonin (0.99%), respectively. The crude Scutellaria extract did not show significant anti-proliferative effect, apoptosis induction and cell cycle arrest in RPMI-8226 within the concentrations of $1-75{\mu}g/mL$. However, the ethanolic extract, baicalein, wogonin and baicalin reduced the side population cells in RPMI-8226, and data showed that baicalein and wogonin had stronger inhibitory effects. Correspondingly, they also exhibited significant effects on decreasing the expression level of ABCG2 protein in RPMI-8226 in vitro. Conclusions: Our results for the first time demonstrated a novel mechanism of action for Scutellaria extract and its main active flavonoids, namely targeting SP cells by modulating the expression of ABCG2 protein. This study provides an insight for new therapeutic strategies targeting cancer stem cells of multiple myeloma.

Evaluation of NDE Characteristics for Measuring the Painting Thickness in Wind Energy Turbine Power Blades Based on Ultrasonic Wave Simulation

Gui-Lin Zhang,염윤택,김선규,조영태,우용득,임광희 한국생산제조학회 2022 한국생산제조학회지 Vol.31 No.3

In recent years, wind energy turbine blades have been manufactured using composite materials. Post manufacturing, these blades are painted with special coatings to protect the surface. Subsequently, they are generally tested by breaking a specimen and checking the uniform coating state using an optical microscope. A non-destructive technique is considered as an inspection tool; therefore, ultrasonic techniques are widely selected to measure thin painting thicknesses. In this study, the NDE (Nondestructive evaluation) CIVA package (CIVA 2020 software, NDE CIVA) was adopted for ultrasound simulation. The package was used to propose parameters, which brings the measurement of painting thickness based on the non-destructive ultrasonic waves. An optimal technique was also proposed with a use of the time and amplitude reflected from the surface and the back in the samples according to the blade painting thicknesses.

Clinical Outcomes of Basaloid Squamous Cell Carcinoma of the Esophagus: A Retrospective Analysis of 142 Cases

Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.

Wave propagation in a FG circular plate via the physical neutral surface concept

Gui-Lin She,Hao-Xuan Ding,Yi-Wen Zhang 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.82 No.2

In this paper, the physical neutral surface concept is applied to study the wave propagation of functionally graded (FG) circular plate, the wave equation is derived by Hamiltonian variational principle and the first-order shear deformation plate model. Then, we convert the equations to dimensionless equations. The exact solution of wave propagation problem is obtained by Laplace integral transformation, the first order Hankel integral transformation and the zero order Hankel integral transformation. The results obtained by the current model are very close to those obtained in the existing literature, which indicates the correctness and reliability of this study. Moreover, the effects of the functionally graded index parameters and pore volume fraction on the wave propagation are also discussed in detail.

Effects of Inclination Angle on Buckling of Continuous Composite Bridges with Inclined Parabolic Arch Ribs

Chengzhong Gui,Junqing Lei,Teruhiko Yoda,Weiwei Lin,Yongshui Zhang 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.2

The effects of the inclination angle on the buckling of continuous composite bridges (CCBs) with parabolic arch ribs remain to be elucidated. Static tests were conducted on an on-site prototype of a CCB with parallel arch ribs to verify the validity of proposed numerical finite element method models of CCBs with inward and outward arch ribs. We found that the expressions for the equivalent slenderness ratios and buckling coefficients should consider the interactions between the in-plane and outof- plane buckling, given the inclination of the arch ribs. With respect to the buckling modes, the arch ribs of all the models buckled in the first lateral mode. In addition, two types of interactions were observed between the buckling modes, namely, that between in-plane and out-of-plane buckling and that between global and local buckling; these were affected by the inclination angle, especially in the case of the CCB with the outward arch ribs.

Resolution of Racemic Ketoprofen in Organic Solvents by Lipase from Burkholderia cepacia G63

Xiang-lin Zhang,Tao Liu,Li Xu,Xiaohua Gui,Feng Su,Yunjun Yan 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6

A lipase from the Burkholderia cepacia strain G63 immobilized on resin was used for the resolution of ketoprofen. To study its catalytic properties in enantioselective esterication, different alcohols and solvents were tested to select the most suitable acyl acceptor and reaction medium. Compared with the low activity of the free lipase, the enzyme activity and E value of the immobilized lipase were significantly enhanced. The enantioselectivity of the immobilized lipase could also be markedly improved by adding a small amount of 18-crown-6. RSM was employed to optimize the reaction parameters. The optimal reaction conditions were: reaction time 22.50 h, additives dosage 0.4322 g (0.33 mmol/mL), and substrate molar ratio 54.11:1. Under optimal conditions, the maximal E value was up to 10.01, which exhibited a better enantioselectivity than some commercial lipases, such as Novozym 435,Lipozyme RM IM and LipozymeTL IM.

Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts antiinflammatory effect by direct inhibiting toll like receptor 4 signaling pathway

Hong-Lin Xu,Guang-Hong Chen,Yu-Ting Wu,Ling-Peng Xie,Zhang-Bin Tan,Bin Liu,Hui-Jie Fan,Hong-Mei Chen,Gui-Qiong Huang,Min Liu,Ying-Chun Zhou 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1

Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-a, IL-6 and IL-1b. Additionally, P. ginseng blocked fluorescence-labeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-kB (NF-kB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/MD2 complex and GRo (KD value of 1.16 × 10<SUP>-9</SUP> M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-kB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.

Corrigendum to "Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts anti-inflammatory effect by direct inhibiting toll like receptor 4 signaling pathway" [J Ginseng Res 46 (2022) 156-166]

Xu, Hong-Lin,Chen, Guang-Hong,Wu, Yu-Ting,Xie, Ling-Peng,Tan, Zhang-Bin,Liu, Bin,Fan, Hui-Jie,Chen, Hong-Mei,Huang, Gui-Qiong,Liu, Min,Zhou, Ying-Chun The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.2

Nonlinear harmonic resonances of spinning graphene platelets reinforced metal foams cylindrical shell with initial geometric imperfections in thermal environment

Yi-Wen Zhang,Gui-Lin She 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.5

This paper reveals theoretical research to the nonlinear dynamic response and initial geometric imperfections sensitivity of the spinning graphene platelets reinforced metal foams (GPLRMF) cylindrical shell under different boundary conditions in thermal environment. For the theoretical research, with the framework of von-Karman geometric nonlinearity, the GPLRMF cylindrical shell model which involves Coriolis acceleration and centrifugal acceleration caused by spinning motion is assumed to undergo large deformations. The coupled governing equations of motion are deduced using Euler-Lagrange principle and then solved by a combination of Galerkin’s technique and modified Lindstedt Poincare (MLP) model. Furthermore, the impacts of a set of parameters including spinning velocity, initial geometric imperfections, temperature variation, weight fraction of GPLs, GPLs distribution pattern, porosity distribution pattern, porosity coefficient and external excitation amplitude on the nonlinear harmonic resonances of the spinning GPLRMF cylindrical shells are presented.

On snap-buckling of FG-CNTR curved nanobeams considering surface effects

Yuan Yuan Zhang,Yu X. Wang,Xin Zhang,Huo M. Shen,Gui-Lin She 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.3

The aim of this paper is to analyze the nonlinear bending of functionally graded (FG) curved nanobeams reinforced by carbon nanotubes (CNTs) in thermal environment. Chen-Yao’s surface elastic theory and geometric nonlinearity are also considered. The nanobeams are subjected to uniform loadings and placed on three-parameter substrates. The Euler-Lagrange equations are employed to deduce the equations of equilibrium. Then, the asymptotic solutions and boundary value problems are analytically determined by utilizing the two-step perturbation technique. Finally, the effects of the surface parameters, geometric factors, foundation stiffness, volume fraction, thermal effects and layout type of CNTs on the nonlinear bending of the nanobeams are discussed.