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이경진 조선대학교 에너지.자원신기술연구소 1999 에너지·자원신기술연구소 논문지 Vol.21 No.1
The objects of the study are to develop conceptual design tool of fusion reactor divertor, and to develop assessment tool for ITER. In the beginning of 1988, conceptual design of ITER was started, and had finished in 1990. Through the study, several important issues were found. Some of them are related with divertor design. This research is dedicated to solve several problems related with divertor cooling system. FEM code to analyze thermal conduction and convection has been developed, and the thermal analysis method of divertor design has been syudied, and characteristics of coolant materials have been studied and analyzed, and feasibility studies of coolant options has been performed.
Lee, Goung Jin,Kim, Soong Pyung CHOSUN UNIVERSITY 1997 Basic Science and Engineering Vol.1 No.2
Recently, the steam injector concept is actively studied to enhance the safety and the reliability of the high pressure water injection system. Even though the basic concept of steam injector is studied by several researchers, the analysis model for steam injector component is ot well developed. Most of researches are performed by using a simple control volume approach, so the accurate prediction of a steam injector performance is not still possible. In this paper, a steam injector performance is nanalyzed by using the five equation two fluid model. Also,a steam nozzle is analyzed by assuming compressible isentropic flow. When a shock wave is occurred inside the nozzle, a shock jump condition is used to consider the sudden change of entropy. By using a conventional SIMPLE algorithm, governing equations are discretized and solved. To understand the sondensation process quantitatively, sensitivity studies are performed using the developed computer code package. To validate and verify the developed method, results are compared against the experimental data of ANL. Compared with the former control volume approach of NL, the developed method gives fairly good results. According to sensitivity studies, most of errors are from the prediction error of steam nozzle exit velocities. By adopting the two fluid model for a steam nozzle analysis, accuracy will be improved greatly.
A STUDY ON THE SIMULTANEOUS MEASUREMENTS OF BETA EMITTING ISOTOPES
Lee, Goung Jin,Kim, Seoung Pyung 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.3
Beta radiation is measured for an environmental monitoring purpose or for an internal radiation exposure monitoring of nuclear power plant's worker. In korea, strontium 89 and strontium 90 is measured for an environmental monitoring purpose. Also tritium and carbon 14 contained in urine is measured for an internal radiation exposure monitoring of nuclear power plant's worker. Because above isotopes emits low energy beta radiations having a wide range of energy, very complicated isotope separation preprocess is needed. In this study, two mixed beta emitting isotopes are measured simultaneously using a liquid scintillation counter(LSC) and analyzed by using a developed statistical method. Banded least square method is used to analyze the mixed spectrum, and the goodness-of-fitness test is proposed . Test results show that the developed procedure can be very useful for analyzing a mixed beta emitting isotopes.
( Mi Jin Lee ),( Goung Ran Yu ),( Hua Lee ),( Jun Zhang ),( Yun A Kim ),( Dae Ghon Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: NM23 is a family of structurally and functionally conserved proteins known as nucleoside diphosphate kinases (NDPK). NM23-H1, NM23-H2 and NM23- LV are expressed abundantly in HCC. NM23-H2 is a basic protein recently identifi ed as the human PuF factor, which is a transcriptional activator of the c-Myc proto-oncogene. Although the NM23 protein is implicated as a metastasis suppressor, the role of NM23 appears to be less understood. Thus, the aim of this study is to examine functional role and mechanism of NM23 involved tumorigenesis in HCC. Methods: We examined the NM23-H1, H2 and LV mRNA expression in HCC by Realtime- PCR analysis and NM23-H1, H2 and LV protein expression in HCC by immunoblot and immunohistochemistry. Focus formation and anchorage-independent growth were examined in stable cell lines expressing NM23 using soft agar. Using overexpression of NM23 by adenoviral system, the molecular mechanism of NM23 mediated tumor cell growth was assessed in experimental cell culture and in vivo animal model. Results: The level of NM23 expression in tumor tissues and the surrounding matrix appeared to be independent of etiology and tumor differentiation. Ectopic expression of NM23-H2 in NIH3T3 fi broblasts and HLK3 hepatocytes enhanced focus formation, and allowed anchorage-independent growth. Overexpression of NM23 results in increased tumor cell proliferation, which is relevant to activation of AKT and extracellular signal-regulated kinase (ERK)1/2 phosphorylation. The NIH3T3 fi broblasts and HLK3 hepatocytes stably expressing NM23-H2 produced tumors in athymic mice. Lentiviral delivery of NM23-H2 shRNA inhibited tumor growth of xenotransplanted tumors produced from HLK3 cells stably expressing NM23-H2. Conclusions: These results indicate that NM23 may be pro-oncogenic and involved in hepatocarcinogenesis through AKT/ERK signaling pathway. Therefore, this pathway may be an useful target for HCC treatment.
Basic, HCC basic : O-034 ; Pro-oncogenic NM23-H2 modulates MDM2 expression in hepatocarcinogenesis
( Mi Jin Lee ),( Goung Ran Yu ),( Hua Lee ),( Sang Wook Kim ),( Pei Pei Hao ),( In Hee Kim ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: NM23 is a family of structurally and functionally conserved proteins known as nucleoside diphosphate kinases (NDPK). NM23-H1 and NM23-H2 are expressed abundantly in HCC. NM23-H2 is a basic protein recently identified as the human PuF factor, which is a transcriptional activator of the c-Myc proto-oncogene. Although the NM23-H1 protein is implicated as a metastasis suppressor, the role of NM23-H2 appears to be less understood. Thus, the aim of this study is to examine functional role and mechanism of NM23-H2 involved tumorigenesis in HCC. Methods: We examined the NM23-H1, H2 and LV mRNA expression in HCC by Realtime-PCR analysis and NM23-H2 protein expression in HCC by immunoblot and immunohistochemistry. Focus formation and anchorage-independent growth were examined in stable cell lines expressing NM23-H2 using soft agar. Using overexpression of NM23-H2 by adenoviral system, the molecular mechanism of NM23-H2 mediated tumor cell growth was assessed in experimental cell culture and in vivo animal model. Results: The level of NM23-H2 expression in tumor tissues and the surrounding matrix appeared to be independent of etiology and tumor differentiation. Ectopic expression of NM23-H2 in NIH3T3 fibroblasts and HLK3 hepatocytes enhanced focus formation, and allowed anchorage-independent growth. Ectopic expression of NM23-H2 induced MDM2 expression. However, MDM2 mRNA and promoter activity was not changed by ectopic expression of NM23-H2, but NM-23H2 interacted with MDM2. The NIH3T3 fibroblasts and HLK3 hepatocytes stably expressing NM23-H2 produced tumors in athymic mice. Lentiviral delivery of NM23-H2 shRNA inhibited tumor growth of xenotransplanted tumors produced from HLK3 cells stably expressing NM23-H2. Conclusions: These results indicate that NM23-H2 may be pro-oncogenic and regulate MDM2 expression in hepatocarcinogenesis. Therefore, this pathway may be an useful target for HCC treatment.
( Mi-jin Lee ),( Goung-ran Yu ),( Hua Lee ),( Yun-a Kim ),( Jun Zhang ),( Dae-ghon Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Erythropoietin-producing hepatocellular receptor tyrosine kinase subtype A2 (EphA2) is an attractive therapeutic target for suppressing tumor progression. EKI1 is coordination complex of zinc and known for its use in treating dandruff and seborrhoeic dermatitis. The aim of this study is to discover novel small molecules to inhibit EphA2 for the treatment of hepatocellular carcinoma and cholangiocarcinoma. Methods: To discover novel and potent EphA2 inhibitors, we performed HTRF (Homogeneous time resolved fluorescence) kinase assay using the chemical library of Korea Chemical Bank and primary screened novel hit compounds. The enhancement of EKI1-mediated apoptosis and autophage were examined using immunoblotting and FACS analysis. Additionally, the antitumor effect of EKI1 was assessed using a mouse model. Results: Thirty-six compounds screened as EphA2 kinase inhibitor by HTRF assay. We validated these substances related to inhibit cell proliferation and cell death. We identified EKI1, a effective theranostics based small molecules. Human hepatocellular carcinoma cell line and cholangiocarcinoma cell lines were treated various concentration of EKI1 for 12h. At low concentration of EKI1, proliferation of these cells was inhibited. At high concentration of EKI1, cell death was induced in these cells. As quantitatively assessed by flow cytometry, apoptosis was induced by EKI1 in cells. We investigated apoptotic signaling by Western blot and observed cleavage or overexpression of pro-caspase-7 and PARP in EKI1 treated cells compared with vehicle. The anti-proliferation effect of EKI was due to an increased autophage, which was confirmed by up-regulation of Autophagy protein 5 (Atg5), BECN and LC3 (autophagosome marker). In addition, EKI1 induced reactive oxygen species (ROS) in JCK and Huh7. We examined EKI1 up-or down-signal transduction pathways to use therapeutic target for HCC and CC. In vivo mouse model, tumor growth was suppressed in EKI1 injected mouse group compared with control group. Conclusions: Our results revealed that autophage and apoptosis are involved in EKI1-mediated tumor cell death. Therefore, the EphA2 kinase inhibitor EKI1 is therapeutic target for hepatocellular carcinoma and cholangiocarcinoma.