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Tissue factor(TF), a primary inhibitor of coagulation protease cascade, was demonstrated by immunohistochemistry using mouse monoclonal antibody to human TF in normal and diseased kidneys. In normal kidneys, TF was localized at glomerular capillary walls. Further localization was perfornmed in serial sections using monoclonal antibodies specific for glomerular epithelial cells. TF positive cells were distributed in the same patterns as those positive to anti human cytokeratin and PHM 5, suggesting its presence in the glomerular in glomerular epithelial cells, In glomerular epithelial cells, most of the TF were found in intracytoplasmic vesicles, and only very small portion of TF were found in peri Golgi areas or in pinocytic pits. Mesangial cells, endothelial cells, tubular epithelium or interstital cells were negative fot TF. I@mmunodouble staing with anti cytokeratin and anti TF antibody confirmed above findings by showing double positivity. In diseased kidneys, the glomerular TF was stained in different patterns,l) proliferative, 2) lobular, 3) capillary. Most of the GNs such as minor change le- sions, membranous nephropathy, membranoproliferative GN, diffuse membranoproliferative GN, focal glomerulosclerosis and IgA nephropathy cases showed capillary staining like in normal glomeruli. Some secondary GNs, such as lupus nephritis(2/9), Henoch Schonlein nephritis(1/1) and transplantation rejection(1/4) also showed cxapillary patterns. Among these, proliferative GNs showed some TF stainings in the mesangium. Lobular patterns were observed in cases with renal lesions combined with metabolic diseases(2/3), or lupus nephritis(6/9), in which TF negative materials or cells were accumulated the mesangium, displacing the TF positive epithelial cells to the periphery. Proliferative types were found in the glomerular tuft in a case with mesangioendothelial proliferative GN, a lupus nephritis, and 3 transplantation rejection cas es, and in crescerts in dne crescentic GN. This type was characterized by TF positive cell proliferation in a glomeruli or in crescent. Fibrin formation within glomeruli is an important pathophysiologic event seen in variety of clinical and experimental conditions associated with renal injury. In this study, TF was demonstrated in vivo in normal kidney tissue, showing TF produced exclusively in glomerular epithelial cells. Reforring from the histologic locations, TF in normal glomeruli could form an hemostatic envelope for the possible capillary injury. In glomerulonephritis, TF showed different patterns, which seemed to reflect its participation not only in coagulation but also in immune mediated renal injury.
Objective: The purpose of this study is to classify couple communication patterns by using stroke, which is a communication concept in Transactional analysis(TA), and find specific stroke exchange methods and patterns unique to couples, and practically for counseling and education. Methods: In order to achieve the research purpose, 4 researchers participated indepth interviews of 14 total participants married male·female group and real marital couple group from Seoul, Gyeonggi, Honam and Gyeongbuk regions and the obtained data was analyzed by a consensus qualitative analysis method(CQR). Results: As a result of the analysis, a total of 21 affiliated categories were drawn in four domains of positive-unconditional stroke, positive-conditional stroke, negitive-unconditional stroke, and negative-conditional stroke in the stroke classification between couples. Conclusion: In this regard, the specific utilization and direction of follow-up studies were presented through discussion, and the implications and limitations of the study were also described. This study has significance that it classified the types of stroke suitable for Korean couples to improve practical in couple therapy and education, and contributed to provide basic data for marital studies through research results.
Polyacetal plastic gears are widely used in the industrial machine elements and often operated about 1m/s pitch line velocity. But there is not enough experiment and research being done in this velocity area. Through the fatigue test of polyacetal gears, we provide the basic test results and estimate the equation of fatigue life from the fatigue life curves. For estimating the equation of fatigue life, we performs the test on two operating conditions. One is not limits the temperature of tooth flank, the other is preserving the temperature of tooth flank under specific limit. As the result, how the temperature of tooth flank affects the failure and the fatigue life of poly acetal gear are shown and the Unit-load and K-factor are determined which are need in the design of polyacetal plastic gear.
1986년 12월부터 87년 11월까지 채집된 애응애과 응애를 분류학적으로 정리한 바 Brevipalpus속의 B.californicus와 B.ovovatus 2종과 다음의 한국4미기록종을 얻었다: Aegyptobia nothus Pritchard and Baker,Pentomerismus taxi Hailer, P. oregonensis McGregor,Brevipalpus lewisi McGregor. 따라서, 한국산 애응애과 응애는 Tenuipalpus속의 T.zhizhilashiliae(감나무애응애)를 포함하여 4속 7종이 보고되는 셈이다. 또한 P. oregonensis,B.californicus,B.ovovatus 및 B.lewisi에 대하여 polyacrylamide slab gel electrophoresis로 esterase와 alkaline phosphatase pattern을 조사한 바 esterase는 band수와 이동도에서 종과 속간에 뚜렷한 차이를 보였다. Six species of false spider mites were collected and ciassifled in South Korea. Among them, a taxonomic description was carried out on the following four species new to Korea: 1. Aegyptobia nothus Pritchard and Baker, 2. Pentomerismus taxi (Hailer), 3. P. oregonensis McGregor, 4. Brevipalpus lewisi McGregor. And also esterase and alkaline phosphatase patterns obtained by polyacrylamide slab gel electrophoresis were compared on some spades. Esterase rymogram showed difference among species in band number and mobility.