Reference Map of Soluble Protenis from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis

김곤섭,Eung-goo Lee,Yong-hwan Kim,정태성,Yong-seung Shin,Gee-wook Shin,Hui-guen Cha,Mi-rim Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis byTwo-Dimensional ElectrophoresisMi-rim Park, Eung-goo Lee, Yong-hwan Kim, Tae-sung Jung, Yong-seung Shin, Gee-wook Shin, Hui-guen Cha and Gon-sup Kim*Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, KoreaReceived May 17, 2003 / Accepted July 23, 2003J. Vet. Sci. (2003), 4(2), 143-149JOURNAL OFVeterinaryScience*Corresponding author: Gon-sup Kim Laboratory of Veterinary Biochemistry, Collage of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701 Korea Tel: +82-55-751-5823, Fax: +82-55-751-5803 E-mail: gonskim@nongae.gsnu.ac.kr

담관 낭선암 1례

박건욱,홍기봉,정재열,노명환,한상영,최석렬,신우원,나서희 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.1

Mo-silicide의 확산 메카니즘에 관한 연구

박종윤,이상균,한기평,이재욱 성균관대학교 1991 論文集 Vol.42 No.1

n-type Si water(100) 위에 Mo를 1000Å을 증착한 후 400℃에서 120분간 pre-annealing(예비 열처리)하고 기존의 FA(Furnace Annealing) 방법과 본 실험실에서 개발, 제작한 RTA(Rapid Thermal Annealing) 장치로 post-annealing하여 hexagonal MoSi_2와 tetragonal MoSi_2를 형성하였다. 이는 XRD로 phase를 확인하였으며 AES depth profile를 이용하여 Si의 확산 메카니즘을 조사하였다. 기판의 온도가 400℃인 경우 MoSi_2의 형성이 용이하고 표면의 grain의 morphology 또한 FA방법에 비해 RTA방법이 크고 균일하게 형성되고 pre-annealing한 시편이 균일한 grain을 형성함을 알았다. 이는 grain의 morphology가 확산에 커다란 영향을 미치고 있음을 암시하고 있다. Mo films with a thickness of 1000Å were prepared on n-type Si(100) substrates by electron beam evaporation and heated to form the MoSi_2 of the hexagonal-and tetragonal-phase by the FA(Furnace Annealing) and RTA(Rapid Thermal Annealing) methods. The phases of Mo-silicide were confirmed by XRD. AES depth profiles were performed to obtain the Si distribution by the diffusion. Also, the change of MoSi_2 surface morphology was analyzed by SEM and EDX to investigate the mechanism of diffusion. When the substrate temperature was 400℃, well defined MoSi_2 was formed easily, the morphology of grain was distributed largely and uniformly. And it was found that the performance of the pre-annealing before post-annealing lead to lower about 150℃ for the formation temperature of very stable MoSi_2.

연속파형 Nd:YAG 레이저에 의한 인코넬 600의 용접 공정변수의 연구

배신철,유영태,송성욱,신호준,나기대 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.1

The laser welding process is one of the most advanced manufacturing technologies owing to its high speed and deep penetration. Welding characteristics of Inconel 600 Alloy using a continuous wave Nd:YAG laser are experimentally investigated. The major process parameters studied in the present laser welding experiment were position of focus, laser power, travel speed. The gap and offset maintained as small as possible. Optical microscope were used to investigate the microstructures of the welded zone. The follow conclusions can be drawn the laser power and travel speed have a pronounced effect the fusion zone size and shape.

Prevalence of plasmid-mediated quinolone and tetracycline resistance genes in Aeromonas strains isolated from eel (Anguilla japonica) and ornamental fish

( Gee-wook Shin ),( Jun-hwan Park ),( Hui-ju Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2

This study investigated the genetic determinants of plasmid-mediated antibiotic resistance (PMAR) to quinolones and tetracycline in 106 Aeromonas strains isolated from eel (Anguilla japonica, 70 strains) and ornamental fish (36 strains) in Korea. Quinolones and tetracycline resistance phenotypes were found to be widely distributed throughout the both fish groups. However, the prevalence of qnr and tet genes was higher in ornamental fish strains than in eel strains (42.9% vs. 86.1% for qnr and 51.4% vs. 69.4% for tet). In addition, the profiling of the present genetic determinants revealed the dominance of qnrS, tetA, tetE and tetE+qnrS genes for eel strains but of tetA+qnrS qnrS and tetE+qnrS genes for ornamental fish strains. These results indicate that aquaculture and related industries could be a major threat to public health due to the possible spread of PMAR.

저압터빈 최종단 블레이드 손상해석

송기욱(Gee Wook Song),최우성(Woo Sung Choi),김완재(Wan Jae Kim),정낭근(Nam Gun Jung) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5-2

저압터빈 최종단 블레이드 손상해석

송기욱(Gee Wook Song),최우성(Woo Sung Choi),김완재(Wanjae Kim),정남근(Nam Gun Jung) 대한기계학회 2013 大韓機械學會論文集B Vol.37 No.12

증기터빈의 터빈 블레이드는 발전소 핵심설비 중 하나로, 로터의 디스크에 결합되어 회전함으로써 증기 에너지를 기계적 에너지로 변환시켜주는 역할을 하고 있다. 최근 터빈의 잦은 기동정지로 인해 블레이드 회전에 따른 원심하중이 반복적 작용하고 이에 따른 저압 증기터빈 최종단 블레이드의 손상이 자주 보고되고 있다. 본 논문에서는 터빈 블레이드에 발생되는 손상을 분석하여 블레이드에 발생되는 저주기 피로수명을 평가하였다. 증기터빈 최종단 블레이드의 균열발생 수명을 결정하기 위해 유한요소법으로 계산한 탄성응력에 Neuber’s rule을 적용하여 진변형율 진폭을 계산하였으며, 예측된 수명과 블레이드 실제 기동정지횟수가 잘 일치됨을 보였다. A steam turbine blade is one of the core parts in a power plant. It transforms steam energy into mechanical energy. It is installed on the rim of a rotor disk. Many failure cases have been reported at the final stage blades of a low-pressure (LP) turbine that is cyclically loaded by centrifugal force because of the repeated startups of the turbine. Therefore, to ensure the safety of an LP steam turbine blade, it is necessary to investigate the fatigue strength and life. In this study, the low cycle fatigue life of an LP steam turbine blade is evaluated based on actual damage analysis. To determine the crack initiation life of the final stage of a steam turbine, Neuber’s rule is applied to elastic stresses by the finite element method to calculate the true strain amplitude. It is observed that the expected life and actual number of starts/stops of the blade were well matched.

Proteome analysis between diverse phenotypes of Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium)

Shin, Gee-Wook,Cha, In-Seok,Lee, Woo-Won,Nho, Seong-Won,Park, Seong-Bin,Jang, Ho-Bin,Kim, Yong-Hwan,Jung, Tae-Sung The Korean Society of Veterinary Science 2010 大韓獸醫學會誌 Vol.50 No.4

Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.

Comparison of antigenic proteins from <i>Lactococcus garvieae</i> KG (−) and KG (+) strains that are recognized by olive flounder (<i>Paralichthys olivaceus</i>) antibodies

Shin, Gee-Wook,Nho, Seong-Won,Park, Seong-Bin,Jang, Ho-Bin,Cha, In-Seok,Ha, Mi-Ae,Kim, Young-Rim,Dalvi, Rishikesh S.,Joh, Seong-Joon,Jung, Tae-Sung Elsevier 2009 Veterinary microbiology Vol.139 No.1

<P><B>Abstract</B></P><P><I>Lactococcus garvieae</I> is an important etiological agent of lactococcosis in various fish species including olive flounder (<I>Paralichthys olivaceus</I>). In this study, proteomic and immunoproteomic analyses were employed to compare the antigenic profiles of strains KG9408, MS93003, and NSS9310 strains of <I>L. garvieae</I>. Proteomic analysis using two-dimensional gel electrophoresis (2-DE) revealed differences in five protein spots among the different <I>L. garvieae</I> strains. In immunoproteomic analysis, there was a significant difference in the 2-DE immunoblot profiles of the <I>L. garvieae</I> strains using sera collected from fish surviving infection with either <I>L. garvieae</I> strains KG9408 or NSS9310. These sera reacted with 8 and 7 unique antigenic protein spots, respectively. Heat shock protein (HSP) 70 and DNA-directed RNA polymerase were among the specific antigens recognized by the anti-NSS9310 serum. In addition, the anti-NSS9310 and anti-KG9408 olive flounder sera reacted with 25 common antigenic protein spots of all the <I>L. garvieae</I> strains, which included elongation factor (EF)-Tu, arginine deiminase (AD), inosine-5′-monophosphate dehydrogenase (IMPD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphomannomutase (PMM), <SMALL>L</SMALL>-lactate dehydrogenase (<SMALL>L</SMALL>-LDH), 6-phosphofructokinase and UDP-galactose 4-epimerase (UDP-galactose). Based on the present results, the 8 antigens recognized by the anti-KG9408 serum and the 25 common antigens recognized by both sera may serve as potential markers for developing an effective vaccine against this bacterium.</P>

RCM 기반 설비 고장 예측시스템

송기욱(Gee Wook Song),김범신(Bum Shin Kim),최우성(Woo Sung Choi) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.4

발전소를 비롯한 각종 플랜트 설비는 많은 기계설비들로 구성되어 고장이 발생 하였을 경우 언제 어디에서 고장이 발생할지 알기가 쉽지 않으며 예기치 못한 고장으로 설비의 이용률이 감소하게 되면 막대한 경제적 손실이 발생한다. 화력발전소는 각종 계측센서를 설치하여 설비를 상시 감시하고 있으며, 경고발생 이전 지시치의 변화를 관찰하여 설비의 이상상태를 자동 감지하는 신호기반 고장 조기경보 시스템도 개발되고 있다. 그러나 고장경보 시스템은 설비로부터 이상 신호가 발생하는지는 알려주지만 고장원인과 중요도에 대한 정보는 제공하지 않는다. 본 연구에서는 고장분석 기능을 가지고 있는 RCM(Reliability Centered Maintenance)과 고장경보시스템을 연계 하여 설비의 이상신호로부터 고장원인과 정비방법에 대한 정보까지 제공해주는 고장예측시스템을 구축하고자 한다. Power plants consist of many components and equipments, It is hard for operator to know where and when equipments have trouble. Unexpected failure results to decrease availability of plant and to occur large economical Joss. Equipments of power plant are monitored all time by various sensors and instruments. But prevention of trouble is very difficult. Then, engineers are developing many kinds of failure alarm system that can find abnormal status of equipment Failure alarm system just informs abnormal condition of equipment. Operator can't know the cause of trouble and failure parts. This study, we have developed failure prediction system to supply cause of trouble and maintenance method.