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김곤섭,Eung-goo Lee,Yong-hwan Kim,정태성,Yong-seung Shin,Gee-wook Shin,Hui-guen Cha,Mi-rim Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2
Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis byTwo-Dimensional ElectrophoresisMi-rim Park, Eung-goo Lee, Yong-hwan Kim, Tae-sung Jung, Yong-seung Shin, Gee-wook Shin, Hui-guen Cha and Gon-sup Kim*Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, KoreaReceived May 17, 2003 / Accepted July 23, 2003J. Vet. Sci. (2003), 4(2), 143-149JOURNAL OFVeterinaryScience*Corresponding author: Gon-sup Kim Laboratory of Veterinary Biochemistry, Collage of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701 Korea Tel: +82-55-751-5823, Fax: +82-55-751-5803 E-mail: gonskim@nongae.gsnu.ac.kr
연속파형 Nd:YAG 레이저에 의한 인코넬 600의 용접 공정변수의 연구
배신철,유영태,송성욱,신호준,나기대 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.1
The laser welding process is one of the most advanced manufacturing technologies owing to its high speed and deep penetration. Welding characteristics of Inconel 600 Alloy using a continuous wave Nd:YAG laser are experimentally investigated. The major process parameters studied in the present laser welding experiment were position of focus, laser power, travel speed. The gap and offset maintained as small as possible. Optical microscope were used to investigate the microstructures of the welded zone. The follow conclusions can be drawn the laser power and travel speed have a pronounced effect the fusion zone size and shape.
Shin, Gee-Wook,Cha, In-Seok,Lee, Woo-Won,Nho, Seong-Won,Park, Seong-Bin,Jang, Ho-Bin,Kim, Yong-Hwan,Jung, Tae-Sung The Korean Society of Veterinary Science 2010 大韓獸醫學會誌 Vol.50 No.4
Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.
Shin, Gee-Wook,Lee, Hu-Jang,Jung, Tae-Sung Korean Society of Environmental Health 2003 한국환경보건학회지 Vol.29 No.4
Jinyangho is a natural water supply source of tap water in west Gyeongnam area, but mass mortality of crusian carp occurred during the time of temperature rise in spring. Examinations on diseased fishes were able to isolate four bacteria isolates and then identified the bacteria as a member of Aeromonas sp. Challenge experiment with mirror carp (Gyrinus carpiospecilaris) was proved the virulence, the isolates were in turn believed as the causative agent of mass mortality in Jinyangho.
Shin, Gee-Wook,Nho, Seong-Won,Park, Seong-Bin,Jang, Ho-Bin,Cha, In-Seok,Ha, Mi-Ae,Kim, Young-Rim,Dalvi, Rishikesh S.,Joh, Seong-Joon,Jung, Tae-Sung Elsevier 2009 Veterinary microbiology Vol.139 No.1
<P><B>Abstract</B></P><P><I>Lactococcus garvieae</I> is an important etiological agent of lactococcosis in various fish species including olive flounder (<I>Paralichthys olivaceus</I>). In this study, proteomic and immunoproteomic analyses were employed to compare the antigenic profiles of strains KG9408, MS93003, and NSS9310 strains of <I>L. garvieae</I>. Proteomic analysis using two-dimensional gel electrophoresis (2-DE) revealed differences in five protein spots among the different <I>L. garvieae</I> strains. In immunoproteomic analysis, there was a significant difference in the 2-DE immunoblot profiles of the <I>L. garvieae</I> strains using sera collected from fish surviving infection with either <I>L. garvieae</I> strains KG9408 or NSS9310. These sera reacted with 8 and 7 unique antigenic protein spots, respectively. Heat shock protein (HSP) 70 and DNA-directed RNA polymerase were among the specific antigens recognized by the anti-NSS9310 serum. In addition, the anti-NSS9310 and anti-KG9408 olive flounder sera reacted with 25 common antigenic protein spots of all the <I>L. garvieae</I> strains, which included elongation factor (EF)-Tu, arginine deiminase (AD), inosine-5′-monophosphate dehydrogenase (IMPD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphomannomutase (PMM), <SMALL>L</SMALL>-lactate dehydrogenase (<SMALL>L</SMALL>-LDH), 6-phosphofructokinase and UDP-galactose 4-epimerase (UDP-galactose). Based on the present results, the 8 antigens recognized by the anti-KG9408 serum and the 25 common antigens recognized by both sera may serve as potential markers for developing an effective vaccine against this bacterium.</P>
The Prevalence of <i>Coxiella burnetii</i> Infection in Wild Korean Water Deer, Korea
SHIN, Gee-Wook,KIM, Eun-Ju,LEE, Hae-Beom,CHO, Ho-Seong The Japanese Society of Veterinary Science 2014 The Journal of veterinary medical science Vol.76 No.7
<P><B>ABSTRACT</B></P><P>The aim of this study was to evaluate the prevalence of <I>Coxiella burnetti</I> infection in wild Korean water deer (<I>Hydropotes inermis argyropus</I>) in Korea, by using serology and real-time PCR analyses. One hundred ninety-six sera were collected from 4 provinces and tested for anti-<I>C. burnetii</I> antibody detection, by means of CHEKIT Q fever ELISA kit; and <I>C. burnetii</I> IS1111 insertion sequence detection, by means of real-time PCR. Antibodies were detected in 18 of the 196 (9.18%) serum samples, whereas genomes of <I>C. burnetii</I> were detected in 13 of the 196 (6.63%) serum samples. Based on overall high seroprevalence, the public health implications of these findings are important, because they indicate that asymptomatic seropositive or seronegative wild animals may be consistently shedding <I>C. burnetii</I>. This is the first study of <I>C. burnetii</I> prevalence in Korean water deer in the Republic of Korea that has indicated the presence of infected animals throughout the country.</P>
( Gee-wook Shin ),( Jun-hwan Park ),( Hui-ju Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2
This study investigated the genetic determinants of plasmid-mediated antibiotic resistance (PMAR) to quinolones and tetracycline in 106 Aeromonas strains isolated from eel (Anguilla japonica, 70 strains) and ornamental fish (36 strains) in Korea. Quinolones and tetracycline resistance phenotypes were found to be widely distributed throughout the both fish groups. However, the prevalence of qnr and tet genes was higher in ornamental fish strains than in eel strains (42.9% vs. 86.1% for qnr and 51.4% vs. 69.4% for tet). In addition, the profiling of the present genetic determinants revealed the dominance of qnrS, tetA, tetE and tetE+qnrS genes for eel strains but of tetA+qnrS qnrS and tetE+qnrS genes for ornamental fish strains. These results indicate that aquaculture and related industries could be a major threat to public health due to the possible spread of PMAR.
Shin, Yong-seung,Lee, Eung-goo,Shin, Gee-wook,Kim, Young-rim,Lee, Eun-young,Kim, Jae-hoon,Jang, Hwan,Gershwin, Laurel J.,Kim, Dae-yong,Kim, Yong-hwan,Kim, Gon-sup,Suh, Myung-deuk,Jung, Tae-sung WILEY-VCH Verlag 2004 Proteomics Vol.4 No.11
<P>Antigenic proteins of Neospora caninum (N. caninum) against bovine immunoglobulins M, E, A, and G were investigated by using immunoproteomics. Proteins of N. caninum (KBA-2) tachyzoite lysates separated by two-dimensional gel electrophoresis were transferred to polyvinylidene difluoride (PVDF) membranes, probed with different bovine immunoglobulin class and classified. Antigenic spots recognized were also identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis. 132, 84, 4, and 40 antigenic protein spots were recognized on N. caninum immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. Of these protein spots, the antigenic proteins recognized by either IgM, IgE, and IgG, or IgM and IgG were HSP70, pyruvate kinase, actin, NCDG-1, tubulin α-chain, and putative ribosomal protein S2. On the other hand, IgM, IgE, and IgA reacted with NTPase, HSP60, tubulin β-chain, putative protein disulfide isomerase, enolase, lactate dehydrogenase, serine-threonine phosphatase, 14-3-3 protein homologue, and GRA2 protein. Most of the antigenic proteins identified were associated with the process of invasion, proliferation, and egression of apicomplexans. In our study, HSP70, actin, NTPase, HSP60, pyruvate kinase, enolase, putative ribosomal protein S2, NCDG-1, and GRA2 proteins were found to be immunodominant proteins, which may contribute to the development of diagnostic markers and vaccine.</P>