Effects of Glucagon-like Peptide-2 on Morphology, Proliferation and Enzyme Activity of Intestinal Enterocyte Cells of Weaned Piglets In vitro

Jia, Gang,Jiang, RongChuan,Wang, KangNing Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.8

This study was conducted according to the single-factor design principle to investigate in vitro the effects of different glucagon-like peptide-2 (GLP-2) concentrations (0, $1{\times}10^{-11}$, $1{\times}10^{-10}$, $1{\times}10^{-9}$, $1{\times}10^{-8}$ and $1{\times}10^{-7}$ mol/L) on the morphology, proliferation and enzyme activity of intestinal enterocyte cells of 28-d-old weaned piglets. These cells were primary cultured in 4 pieces of 24-well cell culture plate. After having been grown for 48 h in culture media with hGLP-2, the ileal enterocyte cells of 28-d-old weaned piglets exhibited the typical characteristics of simple columnar epithelium. Compared with the control groups, the quantities of treated cells significantly increased (p<0.05) and their corresponding absorption values in 540 nm (MTT OD) also significantly increased (p<0.01). Likewise, lactic acid concentration, total protein content and protein retention significantly increased (p<0.05). $Na^{+}$, $K^{+}$-ATP enzyme activity was more active (p<0.05), although the activity of alkaline phosphatase, lactic acid dehydrogenase and creatine phosphokinase in culture media significantly decreased (p<0.01). To summarize, the results indicated that GLP-2 in vitro is capable of promoting the proliferation of intestinal enterocyte cells of 28-d weaned piglets, restraining their apoptosis and maintaining the integrity of their morphology.

Effects of Glucagon-like Peptide-2 on Morphology, Proliferation and Enzyme Activity of Intestinal Enterocyte Cells of Weaned Piglets In vitro

Gang Jia,RongChuan Jiang,KangNing Wang 아세아·태평양축산학회 2009 Animal Bioscience Vol.22 No.8

This study was conducted according to the single-factor design principle to investigate in vitro the effects of different glucagon-like peptide-2 (GLP-2) concentrations (0, 1×10-11, 1×10-10, 1×10-9, 1×10-8 and 1×10-7 mol/L) on the morphology, proliferation and enzyme activity of intestinal enterocyte cells of 28-d-old weaned piglets. These cells were primary cultured in 4 pieces of 24-well cell culture plate. After having been grown for 48 h in culture media with hGLP-2, the ileal enterocyte cells of 28-d-old weaned piglets exhibited the typical characteristics of simple columnar epithelium. Compared with the control groups, the quantities of treated cells significantly increased (p<0.05) and their corresponding absorption values in 540 nm (MTT OD) also significantly increased (p<0.01). Likewise, lactic acid concentration, total protein content and protein retention significantly increased (p<0.05). Na+, K+-ATP enzyme activity was more active (p<0.05), although the activity of alkaline phosphatase, lactic acid dehydrogenase and creatine phosphokinase in culture media significantly decreased (p<0.01). To summarize, the results indicated that GLP-2 in vitro is capable of promoting the proliferation of intestinal enterocyte cells of 28-d weaned piglets, restraining their apoptosis and maintaining the integrity of their morphology.

Vibration analysis of new drill string system with hydro-oscillator in horizontal well

Jia-lin Tian,Zhi Yang,You Li,Lin Yang,Chun-ming Wu,Gang Liu,Chang-fu Yuan 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.6

With the growth of oil and gas resource demand, the hydro-oscillator is widely used to enhance the Rate of penetration (ROP) and improve the efficacy in drilling various wells. The vibration model is the key issue of dynamics analysis and optimization of downhole tools. For the vibration analysis of the new drill string system with hydro-oscillator in the horizontal well, based on the design of the new hydro-oscillator and its operation conditions, the kinematics expressions are presented. Combined with the vibration force calculation results of the hydro-oscillator, the dynamics model of the new drill string system is established. Furthermore, the important features of vibration frequency, displacement, velocity and acceleration are discussed in the numerical example calculation results. By comparing the results of the calculation and experiment test, we can verify the correctness of the analysis model. With the hydro-oscillator vibration effect, the static friction between the drill string and wellbore is changed to the dynamic friction, so it can result in a significant increase in run length. At the same time, the ROP can be enhanced with the vibration effect. Moreover, with the parameters’ adjustment according to the operation conditions, the analysis method and model can also provide references to the study of similar downhole tools dynamics or mechanical properties.

PACKET RADIO NETWORK TECHNOLOGY AND ITS DESIGN

Gang, Wang,Lou, Jia Shi 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

Packet Radio Networks have been developed for ten years and more. Today, many users and researchers are interested in this technology. This paper introduces mainly Packet Radio Network technology and some issues about its design. There are many design choices that must be made in the development of a packet radio network. The decisions are dependent on the environment that the network must work in, the requirements for performance and other functionalities, and the cost and other limitations. In addition, as new hardware and software technologies become available, the parameters governing the decisions change and often result in different selections. The intent of this paper is to provide an overview of the design decisions that must be made so as to provide a context for the decisions made in a number of existing and developing packet radio networks.

Rock-breaking analysis model of new drill bit with tornado-like bottomhole model

Jia-lin Tian,Chang-fu Yuan,Lin Yang,Chuan-hong Fu,Gang Liu,Zhi Yang,Chun-ming Wu 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.4

For improving the rock-breaking efficiency of oil and gas drill bits, a new drill bit is presented with tornado-like bottomhole model(named swirling cutting bit). The new drill bit cutter breaks rock with shocking and cutting effect during the drilling process, and theelements on largest ring cross the borehole center with high speed. It can effectively improve the center rock-breaking efficiency. Meanwhile,for all cutters on different rings breaking rock at the same time, it can optimize the rock-breaking volume of each element andimprove the bit service life. To analyze the interaction features between cutters and rock, the position equations are established by thecylindrical coordinates and complex movement principles, and then the velocity and acceleration equations can be obtained. Based on thenumerical example results, this paper analyzes the bottomhole model, the contact section, and the distribution features of velocities andaccelerations on different rings. By analyzing the acceleration results, we can study the failure mechanism of cutters. At the same time,lab experiments test the bottomhole model and rock-breaking features, and it verifies the accuracy of calculation method and equations. Moreover, the analysis method and models are also applicable to other types of bits or composite bits, and the inputting parameters needcorresponding adjustment for different type of bits.

Optimal Objects of Cooperation Selection for Human Activity in Opportunistic Networks

Jia Wu,Zhi-gang Chen,Xi Yi 한국산학기술학회 2014 SmartCR Vol.4 No.2

Randomness and disconnections during transmission in opportunistic networking have some characteristics similar to human activity. Many traditional methods in opportunistic networks, however, have never achieved effective results when applied to human activity. Consequently, this paper establishes and analyzes a structure tree and then, based on count dependability and usability in personal relationships, an optimized cooperation path is selected. Furthermore, the paper presents a new algorithm?the Optimal Cooperation Path Algorithm ?for opportunistic networks. This algorithm solves the problem of how to choose an appropriate path for human activity in opportunistic networks. When tested during simulations, this algorithm achieved good results.

Analysis of Offensive Strategy in Soccer Video

Jia Wang,Haitao Yang,Miao Wang,Gang Liu 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

A novel Real-world trajectory based tactic analysis method is proposed, which can automatically analyze the offensive tactics of soccer game from the perspective of professionals. First, a real-world trajectory extraction method is proposed, and then an offensive pattern recognition method is described based on the definition of the ball states. Our experiments on user study by soccer professionals demonstrate that the defined offensive patterns can be used to analyze soccer tactics effectively in terms of conciseness, clarity and usability.

Synthesis, Structure and Properties of Two Novel 2D Zinc(II) Coordination Polymers based on Fluconazole and Benzene Carboxylic Acid

Gang-Hong Pan,Jing-Niu Tang,Wen-Jia Xu,Peng Liang,Zhong-Jing Huang 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.12

Experimental Study on the Shear Behaviors of PolypropyleneFiber-Reinforced Sand

Gang Li,Jinli Zhang,Jia Liu 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.12

With excellent water permeability and high shear strength S, fiber-reinforced sand (FRS) is an ideal material for subgrades and has promising application prospects. In this study, the FRS were formed by adding the randomly distributed fibers to the sand specimens. Based on the triaxial consolidated drained tests, the effects of factors such as fiber content ζ, fiber length lf, relative density Dr and confining pressure σ3 on the S of FRS were systematically investigated. The test results showed that the S of the FRS increased as ζ, lf, Dr and σ3 increased. Under axial loads, the stress-strain curve of the FRS exhibited a hardening trend. Under specific Dr and σ3, the S increment of the FRS had a strong linear relationship with ζ and lf. The Mohr's stress circle results showed that the FRS possessed not only frictional strength but also high cohesive strength. Based on the triaxial consolidated drained test results, the S of the FRS was calculated using the Zornberg model and Michalowski model. The calculation results showed that the S predicated by the Zornberg model differed significantly from the measured values. However, the S predicted by the Michalowski model were in good agreement with the measured values.

An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress

Jia, Baolei,Lee, Sang-Min,Pham, Bang P.,Cho, Yoon-Seung,Yang, Jae-Kyung,Byeon, Hee-Seop,Kim, Jong-Cheol,Cheong, Gang-Won Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.4

NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to $H_2O_2$ or four-electron reduction of oxygen to $H_2O$. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to ${\beta}$-amyloid and Lewy bodies in neuro-degenerative diseases, including an increase of ${\beta}$-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce $H_2O_2$. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treatment with $H_2O_2$. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.