A Novel Effect of Lipids Extracted from Vernix Caseosa on Regulation of Filaggrin Expression in Human Epidermal Keratinocytes

( Wu Qiao ),( Tinghan Jia ),( Hongjian Gu ),( Ruihua Guo ),( Ken Kaku ),( Wenhui Wu ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.6

Background: Vernix caseosa (VC), which is known as a unique human substance, is a biofilm that covers the skin of most human newborns. VC has many biological functions including anti-infective, skin cleansing and skin barrier repair. Objective: In the study, we purpose to investigate the novel effect of lipids extracted from VC on the regulation of filaggrin (FLG) expression and anti-inflammation in normal human epidermal keratinocyte (NHEK) cells. Methods: The lipids were extracted by chloroform/methanol (Folch method) and the major properties of fatty acid methyl esters were determined with gas chromatography-mass spectrometer. The relative viability of NHEK cells was evaluated by Cell Counting Kit 8 assay. The related expression of skin barrier protein was accessed with real-time quantitative polymerase chain reaction, Western blot and Immunofluorescence in NHEK cells with or without poly (I:C). Meanwhile, the changes of thymic stromal lymphopoietin (TSLP) and tumor necrosis factor alpha (TNF-α) are analyzed by enzyme- linked immunosorbent assay. Results: VC lipids mostly contained saturated and branched chains fatty acids. The expression of mRNA and protein of FLG were significantly increased after the supplement with lipid in NHEK cells. Meanwhile, lipids reversed the inhibition of poly (I:C) on FLG. Moreover, lipids suppressed the over secretion of TSLP and TNF-α induced by poly (I:C). Conclusion: These results indicate that lipids extracted from VC has positive effects on the expression of FLG and anti-inflammation, suggesting that lipids of VC may be used for a reference for novel therapeutic method in reducing and remedying skin disease like atopic disease. (Ann Dermatol 31(6) 611∼620, 2019)

Soft Power of Seoul City - The Perceived Image of Seoul by Chinese Visitors

Jia Lin Wu,Bong Seok Kim People&Global Business Association 2017 Global Business and Finance Review Vol.22 No.3

Seoul is a popular destination for Chinese tourists and Seoul’s image has been a popular topic for scholars in recent years. This study aimed to examine Seoul’s largest consumers, Chinese visitors, and to obtain more understanding of these visitors’ perceptions of Seoul by analyzing Chinese visitors’ perceived image of Seoul and identifying the soft power of Seoul city. The study is based on 103 online photographic representations from 88 blogs identified through the well-known Chinese search engine-Baidu. The study found that Chinese visitors described Seoul as a modernized city with its own authentic culture, historical changes and accessibility. The soft power of Seoul is identified in several aspects, including cultural fascination, a safe environment, tourism attractiveness, and information accessibility. Marketers should take advantage of these soft powers to maintain the tourism industry. Synecdochical features should also be used to enhance the destination image of Seoul. In light of these results, the authors discuss implications for both marketers and researchers as well as directions for further research.

Use of moving substructure element for vibration analyses of a structure due to a moving trolley carrying a swinging mass

Wu, Jia-Jang Techno-Press 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.4

The Constructs of the Lazarus and Folkman’s Stress-Appraisal-Coping Theory as Predictors of Subjective Well-Being in College Students During the Ongoing COVID-19 Pandemic

Jia Rung Wu,Kanako Iwanaga,Fong Chan,Wu Hu,Kaiqi Zhou,Xiangli Chen,Chetwyn C. H. Chan,Timothy N. Tansey 한국상담학회 2023 Journal of Asia Pacific counseling Vol.13 No.2

The COVID-19 pandemic has had significant adverse effects on the mental health of college students around the world. The purpose of the present study was to examine the constructs of Lazarus and Folkman’s stress-appraisal-coping theory as predictors of subjective well-being in Chinese college students during the ongoing COVID-19 pandemic. Participants included 244 Chinese college students. The final hierarchical regression model accounted for 71% of the variance in subjective well-being scores, a large effect size. In the final model, positive stress appraisal, hope, core self-evaluations and resilience remain significant after controlling for other variables. Coping flexibility were significant at the zero-order correlation level and at the step, it was entered into the equation. The effect of perceived stress dissipated in the presence of the positive person-environment contextual variables. Findings provide strong empirical support for the use of positive person-environment contextual variables as a stress management and subjective well-being model for college students during and after the COVID-19 pandemic. Findings can be used to guide counselors, psychologists, and psychiatrists in college health services in the selection of brief screening psychological instruments and empirically supported psychosocial and counseling interventions to help college students develop character strengths, positive attitudes, and coping skills to effectively cope with COVID-related challenges and stressors and possible future outbreaks of novel contagious diseases and crisis situations.

Free vibration analysis of a beam with arbitrarily distributed rigid beam segments using elastic-and-rigid-combined beam element

Wu, Jia-Jang Techno-Press 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.34 No.6

BRCA1 Reversion Mutation Confers Resistance to Olaparib and Camrelizumab in a Patient with Breast Cancer Liver Metastasis

Jia-Ni Pan,Lei Lei,Wei-Wu Ye,Xiao-Jia Wang,Wen-Ming Cao 한국유방암학회 2021 Journal of breast cancer Vol.24 No.5

Reversion mutations are associated with clinical resistance to poly(ADP-ribose) polymerase inhibitors (PARPi). Here, we describe the detection of a BRCA1 reversion mutation in a 39-year-old woman with metastatic breast cancer harboring a heterozygous germline BRCA1 exons 7–8 deletion who received PARPi olaparib combined with immune checkpoint inhibitor camrelizumab as third-line therapy. During progression from the olaparib and camrelizumab combination therapy, we identified via genomic sequencing a novel 7-base pair somatic deletion in BRCA1 (c.617_623delACAAATC). Sequence analyses indicated that this mutation realigned the reading frame of BRCA1, which potentially led to the reversal of its normal function and conferred resistance to PARPi.

DAB Converter Based on Unified High-Frequency Bipolar Buck-Boost Theory for Low Current Stress

Jia-rong Kan,Yao-dong Yang,Yu Tang,Dong-chun Wu,Yun-ya Wu,Jiang Wu 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.2

This paper proposes a unified high-frequency bipolar buck-boost (UHFBB) control strategy for a dual-active-bridge (DAB), which is derived from the classical buck and boost DC/DC converter. It can achieve optimized current stress of the switches and soft switching in wider range. The UHFBB control strategy includes multi-control-variables, which can be achieved according to an algorithm derived from an accurate mathematical model. The design method for the parameters, such as the transformer turns ratio and the inductance, are shown. The current stress of the switches is analyzed for selecting an optimal inductor. The analysis is verified by the experimental results within a 500W prototype.

High Mobility Group Box 1 Protein Is Methylated and Transported to Cytoplasm in Clear Cell Renal Cell Carcinoma

Wu, Fei,Zhao, Zuo-Hui,Ding, Sen-Tai,Wu, Hai-Hu,Lu, Jia-Ju Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: The high mobility group box 1 (HMGB1) protein is a widespread nuclear protein present in most cell types. It typically locates in the nucleus and functions as a nuclear cofactor in transcription regulation. However, HMGB1 can also localize in the cytoplasm and be released into extracellular matrix, where it plays critical roles in carcinogenesis and inflammation. However, it remains elusive whether HMGB1 is relocated to cytoplasm in clear cell renal cell carcinoma (ccRCC). Methods: Nuclear and cytoplasmic proteins were extracted by different protocols from 20 ccRCC samples and corresponding adjacent renal tissues. Western blotting and immunohistochemistry were used to identify the expression of HMGB1 in ccRCC. To elucidate the potential mechanism of HMGB1 cytoplasmic translocation, HMGB1 proteins were enriched by immunoprecipitation and analyzed by mass spectrometry (MS). Results: The HMGB1 protein was overexpressed and partially localized in cytoplasm in ccRCC samples (12/20, 60%, p<0.05). Immunohistochemistry results indicated that ccRCC of high nuclear grade possess more HMGB1 relocation than those with low grade (p<0.05). Methylation of HMGB1 at lysine 112 in ccRCC was detected by MS. Bioinformatics analysis showed that post-translational modification might affect the binding ability to DNA and mediate its translocation. Conclusion: Relocation of HMGB1 to cytoplasm was confirmed in ccRCC. Methylation of HMGB1 at lysine 112 might the redistribution of this cofactor protein.

Highly efficient and ultra-rapid adsorption of malachite green by recyclable crab shell biochar

Jia Wu,Jianwei Yang,Pu Feng,Lishan Wen,Guohuan Huang,Chuanhui Xu,Baofeng Lin 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

In this study, waste crab shell was hydrothermally carbonized and then activated to prepare an effectiveand recyclable adsorbent. The adsorption performance of the adsorbent for different dyes was investigatedthrough adsorption experiments, and the results showed that it had the largest adsorption performancefor malachite green. Furthermore, the adsorption performance and mechanism of malachite greenwere investigated by static and dynamic adsorption method. The crab shell biochar could remove6142.5 mg g1 of malachite green within 3 minutes, indicating that the crab shell biochar could ultrarapidand highly efficient adsorb malachite green. In addition, the malachite green adsorbed on the crabshell biochar could be removed by calcination, which achieved the entire regeneration of the crab shellbiochar. The adsorption of malachite green by crab shell biochar adsorbent conformed to the pseudosecond-order kinetic model, the Dubinin-Radushkevich and Langmuir isotherm adsorption model. Physical and chemical characterization indicated that the adsorption mechanism of malachite greenincluded alkaline fading effect, hydrogen bonding and p-p interaction.

Free vibration analysis of a uniform beam carrying multiple spring-mass systems with masses of the springs considered

Jia-Jang Wu 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.6

The reports regarding the free vibration analysis of uniform beams carrying single or multiple spring-mass systems are plenty, however, among which, those with inertia effect of the helical spring(s) considered are limited. In this paper, by taking the mass of the helical spring into consideration, the stiffness and mass matrices of a spring-mass system and an equivalent mass that may be used to replace the effect of a spring-mass system are derived. By means of the last element stiffness and mass matrices, the natural frequencies and mode shapes for a uniform cantilever beam carrying any number of springmass systems (or loaded beam) are determined using the conventional finite element method (FEM). Similarly, by means of the last equivalent mass, the natural frequencies and mode shapes of the same loaded beam are also determined using the presented equivalent mass method (EMM), where the cantilever beam elastically mounted by a number of lumped masses is replaced by the same beam rigidly attached by the same number of equivalent masses. Good agreement between the numerical results of FEM and those of EMM and/or those of the existing literature confirms the reliability of the presented approaches.