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K. B. R. S. Visarada,G. Shyam Prasad,Monique. Royer 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.5
Sweet sorghum is a climate smart crop with multiple uses. The crop is susceptible to attack by the spotted stemborer, Chilo partellus (Swinhoe). This causes deadheart formation, leading to lodging of plants and consequent high economic losses. Lack of stable sources of resistance make any genetic enhancement through breeding difficult. We report a study to build up host plant resistance using transgenic technology by introducing two different classes of Bt genes (cry1Aa and cry1B) into two elite sweet sorghum genotypes of India (SSV84 and RSSV9). We devised tissue culture methods to suit the genotypes of our interest, SSV84 and RSSV9, and employed two methods of genetic transformation: the particle bombardment and in planta method of Agrobacterium. Modification of in vitro culture methods involved subculture every 3 days in the initial stages of culture and the use of precultured embryos as target tissues. For the in planta method, a floral dip for 1 h in Agrobacterium suspension supplemented with Lcysteine and Tween-20 was used. Sixteen transgenic events were generated; inheritance, integration and stable expression of the transgenes till the T4 generation were confirmed. The amount of Bt Cry1Aa protein at 25–30 days of growth ranged from 24.8 to 72.8 ng/g of fresh leaf tissue. We recorded 78.4 % larval mortality, reduced leaf damage (3.0 out of 9.0) and reduced feeding (41.0 %) over the controls in insect feed assays. Stable inheritance and expression in the in planta-derived transgenics are presented.
R.B. Basavaraj,H. Nagabhushana,G.P. Darshan,B. Daruka Prasad,S.C. Sharma,K.N. Venkatachalaiah 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.51 No.-
Nano research offered new possibilities in surface-based science includes latentfingerprints and lipsprint detection on various surfaces. CdSiO3:Dy3+ nanopowders were fabricated via modifiedsonochemical method. Eccrine prints stained by optimized composition of prepared samples exhibitedhigh sensitivity, low background hindrance on various surfaces compared to traditionalfluorescentpowders. Surface morphologies were studied with different sonication influential parameters. Theestimated average crystallites size and band gap were found to be 22 nm and 5.37 eV respectively. Photometric CIE and CCT values were close to near ultraviolet light with CP of 95% for the preparedcompounds confirms their utility in thefield of optoelectronics
Dileep K G,Pradeep Goutam,Laxmaiah P,Nithin Kumar S,Hari Prasad S V,Nithin B 대한전자공학회 2017 대한전자공학회 학술대회 Vol.2017 No.1
This paper demonstrates the implementation of a QPSK/Time Division Multiple Access (TDMA) satellite receiver using short preamble-based synchronization in the presence of timing, frequency and phase offsets, and with Additive White Gaussian Noise (AWGN). The detection and estimation of synchronization parameters of the received signal, i.e., frequency, phase and symbol timing, are highly dependent on the preamble of the burst. Moreover, computational complexity and acquisition time of the receiver are directly proportional to the preamble length. The implemented receiver uses a 64-bit short preamble for synchronization and gives better bit error rate (BER) performance at low signal to noise ratio (SNR), close to -3dB. The functional verification of the synchronization algorithm is done using Matlab simulations and the algorithm implementation uses fixed-point C. The receiver design has been prototyped for 4 TDMA channels in Texas Instruments (TI) multi-core C66x DSP. The design and implementation of this receiver have been done for C-DOT indigenous satellite project.
Bhatt, Vaibhav D.,Khade, Prasad S.,Tarate, Sagar B.,Tripathi, Ajai K.,Nauriyal, Dev S.,Rank, Dharamshi N.,Kunjadia, Anju P.,Joshi, Chaitanya G. The Korean Society of Veterinary Science 2012 大韓獸醫學會誌 Vol.52 No.4
The expression profiles of inflammatory cytokines viz. interleukins (IL)-6, IL-8, IL-12, granulocyte macrophage-colony stimulating factor, interferon-${\gamma}$ and tumor necrosis factor-${\alpha}$ in response to subclinical mastitis in indigenous cattle breed Kankrej (n = 6), Gir (Bos indicus) (n = 12) and crossbred (Bos taurus${\times}$Bos indicus) (n = 7) were investigated using quantitative real time PCR. Significant correlation (p < 0.05) was observed between total bacterial load and somatic cell count (SCC) in all three breeds of cattle. All the cytokines were observed to be up-regulated compared to cows with healthy quarters, however, level of their expression varied among three breeds of cattle. In Kankrej most cytokines were found to be transcribed to higher levels than in other two breeds; the milk had higher load of bacteria but not so high SCC, implying that Kankrej has a higher inherent resistance against mastitis. The results of present study indicated that mammary glands of crossbred cattle are more sensitive to bacterial infection than indigenous breed of cattle as they elicit immune response at lower bacterial load and result into higher SCC. Research on identification of factors responsible for differentially expressed cytokines profiles and use of cytokines as immunomodulatory tools can pave way for formulating control strategies against bovine mastitis.
Dhananjay Yadav,Meerambika Mishra,Arvind Tiwari,Prakash Singh Bisen,Hari Mohan Goswamy,G.B.K.S. Prasad 질병관리본부 2014 Osong Public Health and Research Persptectives Vol.5 No.3
Objectives: The present study was designed to estimate the prevalence of dyslipidemia and hypertension based on the National Cholesterol Educational Programme Adult Treatment Panel III definition of metabolic syndrome (MetS). The study also focuses on prevalence for MetS with respect to the duration of disease in GwalioreChambal region of Madhya Pradesh, India. Methods: Type 2 diabetic patients (n = 700) were selected from a crosssectional study that is regularly being conducted in the School of Studies in Biochemistry, Jiwaji University Gwalior, India. The period of our study was from January 2007 to October 2009. Dyslipidemia and hypertension were determined in type 2 diabetic patients with MetS as per National Cholesterol Educational Programme Adult Treatment Panel III criteria. Results: The mean age of the study population was 54±9.3 years with 504 (72%) males and 196 (28%) females. The prevalence of MetS increased with increased duration of diabetes in females; however, almost constant prevalence was seen in the males. Notable increase in the dyslipidemia (64.1%) and hypertension (49%) in type 2 diabetic patients were seen. The steep increase in dyslipidemia and hypertension could be the reason for the growing prevalence of diabetes worldwide. The study also noted a close association between age and occurrence of MetS. Conclusion: Individual variable of MetS appears to be highly rampant in diabetic population. Despite treatment, almost half of patients still met the criteria for MetS. Effective treatment of MetS components is required to reduce cardiovascular risk in diabetes mellitus hence accurate and early diagnosis to induce effective treatment of MetS in Indian population will be pivotal in the prevention of cardiovascular disease and type 2 diabetes.
Biological and Medicinal Properties of Grapes and Their Bioactive Constituents: An Update
Yadav, Mukesh,Jain, Shalini,Bhardwaj, Aarti,Nagpal, Ravinder,Puniya, Monica,Tomar, Radha,Singh, Vinod,Parkash, Om,Prasad, G.B.K.S.,Marotta, Francesco,Yadav, Hariom The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.3
The grape is one of the most valued conventional fruits, worldwide. Although most of the parts of the grapevine are useful, primarily, the grape is considered as a source of unique natural products not only for the development of valuable medicines against a number of diseases, but also for manufacturing various industrial products. Over the last few decades, apart from the chemistry of grape compounds, considerable progress has been made towards exploring the biological activities of various grape-derived constituents. Today, it is well established that in addition to serving as food, the grape is a major source of several phytochemicals. The main biologically active and well-characterized constituent from the grape is resveratrol, which is known for various medicinal properties in human diseases. This review discusses the roles of various grape-derived phytochemicals in relation to various diseases.
Biological and Medicinal Properties of Grapes and Their Bioactive Constituents: An Update
Mukesh Yadav,Shalini Jain,Aarti Bhardwaj,Ravinder Nagpal,Monica Puniya,Radha Tomar,Vinod Singh,Om Parkash,G.B.K.S. Prasad,Francesco Marotta,Hariom Yadav 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3
The grape is one of the most valued conventional fruits, worldwide. Although most of the parts of the grapevine are useful, primarily, the grape is considered as a source of unique natural products not only for the development of valuable medicines against a number of diseases, but also for manufacturing various industrial products. Over the last few decades, apart from the chemistry of grape compounds, considerable progress has been made towards exploring the biological activities of various grape-derived constituents. Today, it is well established that in addition to serving as food, the grape is a major source of several phytochemicals. The main biologically active and well-characterized constituent from the grape is resveratrol, which is known for various medicinal properties in human diseases. This review discusses the roles of various grape-derived phytochemicals in relation to various diseases.