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網膜色素上皮細胞의 Lysosomal acid phosphatase의 活性値 測定
김복희,김은경,김윤화,백미화,이지숙,조영숙,황응숙 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-
Measurement of lysosomal enzyme activity in the rabbit eye was effected using acid phosphatase as enzyme markers. Acid phosphatase in the retinal pigment epithelium of the rabbit eye was studied using standard enzymological techniques. The enzyme activity in the lysosomal extract of the pigment epithelium was 54.9±23.0 King-Armstrong Unit/dl.
다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도
주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2
Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.
일단계 핵산 추출을 이용한 사람거대세포바이러스와 돼지내재레트로바이러스의 검출법
김정헌,정은숙,황응수 대한감염학회 2010 감염과 화학요법 Vol.42 No.1
Background: Xenotransplantation is thought to be one of the alternative methods to overcome the shortage of human organs for transplantation. Recipients should be immunosuppressed for graft survival, and thus, there is a need for developing diagnostic modality that can detect diverse infections originating from animals and recipients rapidly, in the early stage, and with high sensitivity using small volume of samples. This study was carried out to develop a fast, simple, and robust technique for the preparation of HCMV DNA and PERV RNA using small volume of samples. Materials and Methods: Nucleic acids were extracted from serially diluted samples with one step extraction method as well as with Qiagen kit. The presence of genomic DNA of human cytomegalovirus (HCMV) and porcine endogenous retrovirus (PERV) was detected by PCR and specific primer set, respectively. RNA of HCMV and PERV was extracted and then detected by RT-PCR and specific primer set, respectively. For absolute quantification of HCMV, standard curve was established by real time PCR. Results: HCMV DNA and PERV RNA were prepared from culture supernatant and cells for PCR or RT-PCR with one step extraction method. It was possible to extract both the DNA and RNA from the samples in about 20 minutes with one step extraction method in a single tube. HCMV and PERV could also be detected by PCR and one step extraction method, respectively. It was also good with small quantity samples. Conclusions: One step extraction method is simpler and faster method than other extraction methods when there are two types of DNA and RNA viruses in one sample. From these results, we could see that the one step extraction method could be very useful in detecting HCMV and PERV rapidly from the pig cells or organ transplanted recipients with a small amount of sample.
김응중,황석하,임승평,이영 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2
The surgically created arteriovenous fistula has become the method of choice in achieving vascular access for maintenance hemodialysis. The authors performed 70 arteriovenous fistulas for angioaccess in 65 patients with CRF at the Department of Thoracic and Cardiovascular Surgery in Chungnam National University Hospital during the period form August 1990 to November 1991. There were 4 cases of early obstructions due to inadequate vein in 2 cases, inadequate artery in 1 case and technical failure in 1 case. In 3 cases except 1 case who was transferred to other hospital, re-AVF were tried with successful outcomes. There were 2 cases of minor complications (wound infection and arm swelling due to venous hypertension) which were managed conservatively with successful outcomes. During the mean follow up of 8 months in 59 patients whore are not expired or lost during follow up, 3 cases of late thrombosis were occured with fistula patency rate of 94.9%. The authors consider the 'anatomical snuffbox' arteriovenous fistula as a primary choice and consider another method if it is not possible.
제강전로슬래그를 이용한 하수슬러지 고화체의 미세구조 분석
李基錫,趙珍奎,李昇玟,金應鎬 弘益大學校 科學技術硏究所 1999 科學技術硏究論文集 Vol.10 No.1
The solidifying effect of solidified digested sewage sludge by converter slag was investigated through microscopic analyses including XRD, SEM and EDS. The XRD analysis of the solidified sludge showed that the main solidification products was CSH; in addition, Ca(OH)₂CAH and Ettringite were observed. The microstructure analysis by SEM showed that as the solidification proceeds, needlelike crystals and honeycomb appeared. The analysis of these crystals by EDS confirmed that the main components were Ca, Si etc. This also confirmed the general notion that the cement hydrate products had a solidifying effect.
油腐加工 중의 기름의 性狀變化 및 油腐貯藏 중의 品質變化
李應昊,鄭淑鉉,金根淑,金喜衍 釜山水産大學校 1981 釜山水産大學 硏究報告 Vol.21 No.2
油腐 튀김過程중 튀김用 기름의 性狀變化를 作業時間 經過에 따라 工場規模로 檢討하고 油腐를 4가지 貯藏方法으로 貯藏하여 貯藏期間에 따른 品質 變化를 實驗한 結果는 다음과 같다. 1. 튀김用 기름의 性狀變化에 있어서 산값과 色調는 低溫튀김솥(110±10℃) 및 高溫튀김솥(200±10℃)의 기름의 경우 모든 作業時間에 따라 增加하였으며 특히 低溫튀김솥의 기름의 變化가 더 컸다. 2. 과산화물값과 TBA값은 低溫튀김솥의 기름의 경우 增加하다가 減少하였으며 高溫튀김솥의 경우는 變化가 거의 없었다. 3. 油腐에서 抽出한 기름의 性狀은 高溫튀김솥의 기름의 영향을 크게 받았다. 4. 貯藏方法에 따른 油腐의 品質變化에 있어서 常溫(26±2℃) 貯藏의 경우 包裝하지 않은 것이 包裝한 것보다 貯藏性이 높았으며 脂肪의 酸敗面에서 볼 때 包裝하지 않은 것은 60日程度 貯藏이 가능하였다. 5. 4±2℃에서 貯藏할 경우에는 貯藏 期間에 따른 脂肪의 酸敗는 적었지만 長期貯藏은 困難하였고 -20℃에서 貯藏할 경우에는 貯藏期間에 따른 品質의 變化가 거의 없었으며 貯藏性이 매우 좋았다.