Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Eun-Young Kwon,Young-Tae Kim,Dae-Eun Kim 한국트라이볼로지학회 2006 KSTLE International Journal Vol.7 No.1

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young’s modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed (20 μm/s) compared to that in the ambient environment. For measuring the Young’s modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young’s modulus could be derived using the Hertz model. The Young’s modulus of living L-929 fibroblast cell was 1.29 ± 0.2 kPa.

Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Kwon Eun-Young,Kim Young-Tae,Kim Dae-Eun Korean Tribology Society 2006 KSTLE International Journal Vol.7 No.1

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed ($20{\mu}m/ s$) compared to that in the ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was $1.29{\pm}0.2$ kPa.

AFM indentation 기법을 이용한 세포의 탄성특성에 관한 연구

권은영(Eun-Young Kwon),김영태(Young-Tae Kim),김대은(Dae-Eun Kim) 한국트라이볼로지학회 2006 한국트라이볼로지학회 학술대회 Vol.2006 No.6

In this work, imaging and investigation of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed(20 ㎛/s) compare to that in ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was 1.29 ± 0.2 kPa.

P087 : Adverse effects of oral cyclosporine in the treatment of various skin diseases

( Hyung Il Kwon ),( Eun Jin Kim ),( Hyun Woo Kim ),( Hyun Chul Park ),( Jeong Eun Kim ),( Joo Yeon Ko ),( Young Suck Ro ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Cyclosporine (CS) has been used for various skin diseases including psoriasis and atopic dermatitis. However, dermatologists are reluctant to use CS in clinics because of the adverse effects. Objectives: To investigate the adverse effects of CS for dermatological uses. Methods: A retrospective study of 1,335 patients with CS treatment was performed. Results: 15.6% of patients showed adverse effects. Gastrointestinal (GI) symptoms were the most common adverse effect. Other adverse effects included hypercholesterolemia, hypertrichosis, headache, hypertension, hypertriglyceridemia in order of frequency. Hypercholesterolemia was more often in men, but GI symptoms and hypertrichosis in women. Hypercholesterolemia, hypertriglyceridemia and GI symptoms were more common in adults, but hypertrichosis in young. GI symptoms and headache occurred more often within three months of CS treatment, but hypertension, hypercholesterolemia and hypertriglyceridemia after three months. Hypertrichosis was more often in group with low initial dose. GI symptoms occurred more often with lower CS cumulative dose group and hypertension and hypertriglyceridemia in higher CS cumulative dose group. Conclusion: Comparing to previous studies, the frequency of adverse effects of CS was proven to be low. Therefore, in dermatologic field, we suggest that CS is relatively safe with periodic follow-up.

장기간 항갑상선제 투여 후 임의 중단 뒤 재복용시 발생한 무과립구증 1 예

김승만;김태영;박수영;배영환;서영경 정은주;김태균;권민정;박정현;이순희 인제대학교 2011 仁濟醫學 Vol.32 No.-

Agranulocytosis, one of the major side effects of antithyroid a gents, probably occurs in less than 0.25% of cases treated with antithyroid agents. It usually occurs within the first several weeks or months after the initiation of therapy, when drug dosage is higher. Most reported cases of agranulocytosis have occurred during the first 3 months of therapy. We describe here a rare case that did not experience the side effect for 6 years of antithyroid agent treatment but presented with agranulocytosis in one month after re-exposure. It suggests that the major side effect such as agranulocytosis can occur in patients who had undergone prior uneventful antithyroid drug therapy.

벼 상온 저장 시기별 쌀 품질 변화

권석주,송영은,권영립,이덕렬,조승현,최동철 원광대학교 2005 論文集 Vol.35 No.-

Increasing concern about a rice quality, it is considered postharvest technology as well as cultivation management as an important factor. Therefore it is necessary to find optimal drying, storage method that did not affected a rice quality. In order to find the optimum storage period of rice, the rice quality was investigated during storage period. The temperature and humidity of storage bin were greatly changed but those of rice were a little do. The germination rate was slowly decreased as the storage period is long. The content of moisture was affected by a room temperature, it was increased at stored rice in rainy season(10-Jul~20-Jul). As storage period is long, the content of amylose, fatty acidity were a just Little changed but did not showed a constant tendency to it. The content of protein was increased and mechanic palatability value was decreased during storage period. The chromaticity of milled rice showed a range of lightness(L); 75.15~71.75, redness(a); 0.21~0.91, yellowness(b); 16.78~19.28 during storage period.

Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.

Resin infiltration을 시행한 법랑질 초기 우식 병소의 특성

김은영,권민석,김신,정태성 大韓小兒齒科學會 2012 大韓小兒齒科學會誌 Vol.39 No.1

The concept of resin infiltration which was born in an innovative philosophy to arrest the incipient caries. However, the structural changes of resin infiltrated lesions have not yet explained completely. The liquid resin might contribute not only to maximizing the penetration but to deteriorating physical stability. This study was performed to examine some physical and histological features of resin infiltrated incipient carious lesions. With the specimen of resin infiltrated lesions, microhardness by nanoindentation in depth profile, morphology of resin tags were revealed after HCl dissolution, and degree of microleakage were assessed. The percentage of microhardness of surface layer and lesion body of untreated specimen to sound enamel was 64.6% and 24.6% respectively, while that of resin-infiltrated lesions was 72.1%, showing significant difference (p<0.05). The resin tags observed under SEM had relatively homogeneous length of 433(282~501) ㎛ on the average. Among 20 specimens for microleakage assessment, 13 specimens showed no leakage while 5 and 2 showed leakage into outer and inner half of lesion respectively. It was thought the infiltrant resin penetrates deeply and homogeneously into lesion body and improves its hardness with relatively good physical stability. 본 연구는 infiltrant resin을 침투시킨 초기 우식 병소의 특성을 평가하기 위해 법랑질의 깊이에 따른 경도를 측정하고, 병 소 내로의 레진 침투양상을 분석하며, 미세누출 정도를 관찰하여 다음의 결과를 얻었다. 건전 법랑질에 대한 초기 우식 법랑질의 경도 백분율은 표층(5~40 ㎛) 64.6%, 병소본체(60~350 ㎛) 24.6%였으며, infiltrant resin을 침투시킨 병소의 경도 백분율은 72.1%로 나타났다. 5~350 ㎛의 전 깊이에서, infiltrant resin을 침투시 킨 병소는 비처치 우식 병소보다 경도가 유의하게 높았지만 정상 법랑질에 비해서는 경도가 유의하게 낮았다(p<0.05). FESEM 관찰 결과 탈회된 법랑질 내부로 비교적 균일하게 침투한 레진 테그가 관찰되었으며, 침투깊이는 433 ㎛(282-501)로 측정되었다. 열순환 처리 후 13개의 치아에서 미세누출이 관찰되지 않았고, 5개에서 병소 외측 절반, 2개에서 병소 내측 절 반까지 미세누출이 관찰되었다.

난소의 장액성 종양에서의 p27^kip1과 Cyclin D1 발현에 대한 조사

권선영,장은숙,권건영,박관규,김수경 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.3

家兎肺 抽出液과 洗滌液의 表面活性度와 In Vitro X線照射가이에 미치는 影響

權得基,朱永恩 慶北大學校 醫科大學 1975 慶北醫大誌 Vol.16 No.1

家兎肺에서 얻은 推出液(extracts)과 洗滌液(washings)의 肺胞表面 活性物質의 活性度와 蛋白含量을 比 較하고, 나아가서 300R, 600R 및 1,000R의 X線을 in Vitro로 照射하여 20℃에서 1,4,8,12 및 24時間에 各各 表面活性度와 蛋白含量을 測定하여 果 다음과 같은 結果를 얻었다. 1. 正常對照群의 肺洗滌液의 最大 및 最小 表面張力, 幅, S^- 및 蛋白含量은 各各 44.1dyne/cm, 8.0dyne/cm, 14.2dyne/cm, 1.38 및 115.8mg/ml이었고 肺推出液의 그것들은 各各 36.8dyne/cm, 13.6dyne/cm, 9.2dyne/cm, 0.91 및 884.7mg/ml이었다. 2. 肺洗滌液이나 肺推出液에서 모두 20℃에서 實驗 24時間까지 肺胞表面活性度나 蛋白含量은 큰 變化를 나타내지 않았다. 3. 300R의 in Vitro X線 照射로서는 各 實驗値가 對照에 比하여 큰 差異를 나타내지 않았으나, 600R 또 는 1,000R의 in Vitro對照로서는 對照群에 比하여 各實驗値에서 若干의 差異를 나타내었으며, 特히 最小 張力에서 그 傾向이 뚜렷하였다. 4. 以上의 結果로서, 肺胞表面活性度測定에는 肺推出液보다 肺洗滌液을 使用함이 더 有利하고 20℃에서 肺洗滌液을 保存하여도 그 活性度나 蛋白含量에 差異가 없음을 알 수 있다. 또한 X線 in Vitro 照射는 肺胞表面活性物質에 큰 變化를 나타내지 않는 것이라고 思料된다. The extraction method of preparing the pulmonary surfactant is crucial in determining and evaluating the surfactant activities in situ. In the present study, two most commonly used methods were compared; namely, the extracts and washings. Also, in an attempt to clarify the effect of X-irradiation on the pulmonary surfactant system, X-ray of 300R, 600R and 1,000R, respectively was irradiated to the lung extracts and lung washings obtained from the rabbit in Vitro, and the pulmonary surfactant activities as well as the protein contents of both extracts and washings were studied at 1, 4, 8, 12 and 24 hours after the irradiation at 20℃. The results obtained are summarized as follows: 1) The maximal and minimal surface tensions, width of the tension-area diagram at the surface area of 40%, stability index and protein content of the lung washings in the non-irradiated normal group was 44.1 dyne/cm, 8.0 dyne/cm, 14.2 dyne/cm, 1.38 and 115.8 mg/ml, respectively. In the lung extracts, the value was 36.8dyne/cm, 13.6dyne/cm, 9.2dyen/cm, 0.911and 884.7mg/ml, respectively. 2) In the normal group, both the lung washings and lung extracts showed no noticeable change in the pulmonary surfactant activities and protein contents upto 24 experimental hours at 20℃. 3) X-irradiation of 300R in Vitro produced no meaningful change in the surfactant activities or protein contents from the control, but 600R and 1,000R of X-irradiation showed a tendency of sligt changes in the surfactant activities from the control with the increased values of the minimal surface tensions. 4) From the above, it may be considered that the lung washings are better suited than the lung extracts for the measurement of the pulmonary surfactant activities. Also, at 20℃, the pulmonary surfactant activities as well as the protein contents do not seem to be altered greatly for at least 24 hours. Finally, X-irradiation in Vitro does not seem to produce any noticeable change in the pulmonary surfactant.