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Dinesh Babu,박수영,김영휘,양홍철,김정애,Dinesh Thapa,이종숙,김아라 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.1
An aqueous extract of Cornus kousa Burg. leaves (ACK) that contained high amount of polyphenols showed significant antioxidant activity against diphenylpicrylhydrazyl (DPPH) radicals and TNF-α-generated reactive oxygen species. ACK at concentrations of 10 and 50 μg/mL significantly inhibited TNF- α-induced adhesion of U937 pre-monocytic cells to HT-29 colon epithelial cells in a concentrationdependent manner. The reduced adhesion by ACK correlated with the suppressed expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8, the major inflammatory bowel disease (IBD)-associated chemokines. Moreover, ACK significantly suppressed TNF-α-induced translocation of redox-sensitive nuclear factor (NF)-κB as well as degradation of cytosolic I-κBα. The effective concentrations of ACK were much lower than that of 5-aminosalicylic acid (3.06 mg/mL), which is an active metabolite of sulfasalazine, a well-known drug used in the treatment of IBD. The results indicate that ACK may provide a potential benefit for the prevention and treatment of inflammatory diseases such as IBD.
Dinesh Babu,Jong Suk Lee,Su-Young Park,따파디네쉬,Mi Kyoung Choi,Ah Ra Kim,박영준,김정애 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6
Oxidative stress and the activation of nuclear factor (NF)-κB play crucial roles in the pathogenesis of inflammatory bowel disease (IBD). In the present study, we examined the effects of the ethanol extract of Platycarya strobilacea Sieb. stem (EPS) on TNF-α-induced monocyte adhesion to HT29 human colon epithelial cells, an initial step of colon inflammation. EPS contained high amount of polyphenols (0.241±0.017 mg of catechin equivalent/g of extract) and showed substantial DPPH radical scavenging activity. In addition, EPS significantly suppressed TNF-α- induced reactive oxygen species (ROS) increase. Moreover, TNF-α-induced monocyte adhesion to HT29 colon epithelial cells was significantly suppressed by EPS in a concentrationdependent manner. The reduced adhesion by EPS was correlated with suppressed expression of MCP-1 and IL-8, the major chemokines in IBD. EPS also prevented the TNF-α-induced nuclear translocation of NF-κB, one of the redox-sensitive transcription factors, in a concentration- dependent manner. Taken together, our results suggest that the anti-oxidant components of EPS prevent TNF-α-induced NF-κB activation, chemokine induction, and monocyte adhesion at the site of intestinal inflammation.
Babu, Dinesh,Lee, Jong-Suk,Park, Su-Young,Thapa, Dinesh,Choi, Mi-Kyoung,Kim, Ah-Ra,Park, Young-Joon,Kim, Jung-Ae 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6
Oxidative stress and the activation of nuclear factor (NF)-${\kappa}B$ play crucial roles in the pathogenesis of inflammatory bowel disease (IBD). In the present study, we examined the effects of the ethanol extract of Platycarya strobilacea Sieb. stem (EPS) on TNF-$\alpha$-induced monocyte adhesion to HT29 human colon epithelial cells, an initial step of colon inflammation. EPS contained high amount of polyphenols ($0.241{\pm}0.017\;mg$ of catechin equivalent/g of extract) and showed substantial DPPH radical scavenging activity. In addition, EPS significantly suppressed TNF-$\alpha$-induced reactive oxygen species (ROS) increase. Moreover, TNF-$\alpha$-induced monocyte adhesion to HT29 colon epithelial cells was significantly suppressed by EPS in a concentrationdependent manner. The reduced adhesion by EPS was correlated with suppressed expression of MCP-1 and IL-8, the major chemokines in IBD. EPS also prevented the TNF-$\alpha$-induced nuclear translocation of NF-${\kappa}B$, one of the redox-sensitive transcription factors, in a concentration-dependent manner. Taken together, our results suggest that the anti-oxidant components of EPS prevent TNF-$\alpha$-induced NF-${\kappa}B$ activation, chemokine induction, and monocyte adhesion at the site of intestinal inflammation.
Dinesh Simkhada,Tae-Jin Oh,Binod Babu Pangeni Hei Chan Lee,Kwangkyoung Liou,Jae Kyung Sohng 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
The deoxysugar biosynthesis gene cluster of calicheamicin contains calS9, which encodes UDP-D-glucose decarboxylase and catalyzes the conversion of UDP-glucuronic acid to UDP-xylose in the presence of NAD+ cofactor. The calS9 was cloned in pET32a(+) and expressed in Escherichia coli BL21(DE3). An enzymatic assay was carried out with the purified CalS9. A one-pot assay was also developed using thymidyl kinase, acetyl kinase, CalS7 and CalS8 to convert UMP to UDP-xylose via UDP-D-glucose and UDP-glucuronic acid. The reaction products were extracted and analyzed by HPLC and ESI-MS for UDP-D-glucose, UDP-glucuronic acid and UDP-xylose, respectively. The deoxysugar biosynthesis of Streptomyces sp. KCTC 0041BP was inactivated by homologous recombination and the S. sp. GerSM2 mutant, which could not produce dihydrochalcomycin, was obtained. calS7, calS8 and calS9 genes were cloned in integrative plasmid pSET152 to generate pBPDS, which was heterologously expressed in S. sp. GerSM2. Finally, the novel glycosylated product, 5-O-xylosyl-chalconolide derivative, in the conjugal transformants was analyzed by LC-MS.
Biosynthesis of Dihydrochalcomycin: Characterization of a Deoxyallosyltransferase (gerGTI)
Binod Babu Pageni,Dinesh Simkhada,오태진,송재경 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.2
Through an inactivation experiment followed by comple-mentation, the gerGTII gene was previously characterized as a chalcosyltransferase gene involved in the biosynthesis of dihydochalcomycin. The glycosyltransferase gerGTI was identified as a deoxyallosyltransferase required for the gly-cosylation of D-mycinose sugar. This 6-deoxyhexose sugar was converted to mycinose, via bis-O-methylation, following attachment to the polyketide lactone during dihydrochalco-mycin biosynthesis. Gene sequence alignment of gerGTI to several glycosyltransferases revealed a consensus se-quence motif that appears to be characteristic of the en-zymes in this sub-group of the glycosyltransferase family. To characterize its putative function, genetic disruption of gerGTI in the wild-type strain Streptomyces sp. KCTC 0041BP and in the gerGTII-deleted mutant (S. sp. ΔgerGTII), as well as complementation of gerGTII in S. sp. ΔgerGTII-GTI, were carried out, and the products were analyzed by LC/MS. S. sp. ΔgerGTII-GTI mutant produced dihydrochal-conolide macrolide. S. sp. ΔgerGTI and S. sp. ΔgerGTII-GTI complementation of gerGTII yielded dihydrochalconolide without the mycinose sugar. The intermediate shows that gerGTI encodes a deoxyallosyltransferase that acts after gerGTII.
Biosynthesis of Dihydrochalcomycin: Characterization of a Deoxyallosyltransferase(gerGTI)
Pageni, Binod Babu,Simkhada, Dinesh,Oh, Tae-Jin,Sohng, Jae-Kyung Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.2
Through an inactivation experiment followed by complementation, the gerGTII gene was previously characterized as a chalcosyltransferase gene involved in the biosynthesis of dihydochalcomycin. The glycosyltransferase gerGTI was identified as a deoxyallosyltransferase required for the glycosylation of D-mycinose sugar. This 6-deoxyhexose sugar was converted to mycinose, via bis-O-methylation, following attachment to the polyketide lactone during dihydrochalcomycin biosynthesis. Gene sequence alignment of gerGTI to several glycosyltransferases revealed a consensus sequence motif that appears to be characteristic of the enzymes in this sub-group of the glycosyltransferase family. To characterize its putative function, genetic disruption of gerGTI in the wild-type strain Streptomyces sp. KCTC 0041BP and in the gerGTII-deleted mutant (S. sp. ${\Delta}$gerGTsss, as well as complementation of gerGTII in S. sp. ${\Delta}$gerGTss-GTs, were carried out, and the products were analyzed by LC/MS. S. sp. ${\Delta}$gerGTss-GTs mutant produced dihydrochalconolide macrolide. S. sp. ${\Delta}$gerGTs and S. sp. ${\Delta}$gerGTss-GTs complementation of gerGTII yielded dihydrochalconolide without the mycinose sugar. The intermediate shows that gerGTI encodes a deoxyallosyltransferase that acts after gerGTII.
Finite Element Modeling of Beam with Piezoelectric/Piezomagnetic Sensors under Uniform Temperature
Dhanasekaran Rajagopal,A. Kumaravel,S. Arunprasath,M. Dinesh Babu,S. Elayaraja 한양대학교 청정에너지연구소 2024 Journal of Ceramic Processing Research Vol.25 No.1
The present study aims to investigate the behaviour of mild steel beams subjected to multi-phase magnetoelectroelastic,piezoelectric, or magnetostrictive patches, taking into account the effects of temperature. A finite element method wasemployed to analyze the electric and magnetic potential of the structure while considering the coupling effects. The findingsof this study could provide valuable insights into the behaviour of such structures under varying temperature conditions andcontribute to the development of advanced technologies in the field of material science and engineering. Under homogeneoustemperature load, the current formulation shows the ability to anticipate the thermal deformation and sensor behaviour of thepiezoelectric/ piezo magnetic, magnetostrictive patches. A distinct variation characterizes the positioning of the sensor layer inthe beam, and the upper surface of the layer is plotted with transverse displacement, electric potential, and magnetic potentialalong its length. A comparative numerical analysis was conducted to assess the behaviour of multiphase magneto-electroelastic,magnetostrictive, and piezoelectric sensor materials concerning magnetic and electric potential. The investigation hasbeen conducted under various boundary conditions.