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Dennis K. Gomez,Casiano H. Choresca Jr,Ji Hyung Kim,Se Chang Park 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1
Sensitivity of diagnostic methods for detection HPV infection at earlier stages of shrimp is very important for the prevention of widespread and losses in shrimp hatcheries. The earliest postlarval stage detected with HPV infection was 3 days old (PL₃ The presence of hypertrophied nucleus in the hepatopancreas tubule epithelial cell diagnostic of HPV intranuclear inclusion body is indicative of HPV infection. Histological method (H&E) was significantly (P < 0.01) sensitive than impression smear in the diagnosis of HPV infection in Penaeus monodon postlarvae (PL₃ to PL₂₉) reared at two stocking densities (25 and 50 PL/liter). This study showed low diagnostic suitability of Giemsa impression smear and advantage of histological method when HPV infection prevalence is low. There was no significant increase in the detection rate of HPV infection in postlarval stages at two rearing densities.
김지형,Dennis K. Gomez,Toshihiro Nakai,박세창 대한수의학회 2010 JOURNAL OF VETERINARY SCIENCE Vol.11 No.1
In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.
Isolation of Photobacterium damselae subsp. damselae from zebra shark Stegostoma fasciatum
Han, Jee Eun,Gomez, Dennis K.,Kim, Ji Hyung,Choresca, Casiano H. Jr.,Shin, Sang Phil,Baeck, Gun Wook,Park, Se Chang The Korean Society of Veterinary Science 2009 大韓獸醫學會誌 Vol.49 No.1
The zebra shark Stegostoma fasciatum which had been reared in the commercial aquaria was found dead and submitted for postmortem examination. A pure bacterial culture was isolated from pale and enlarged liver. The analysis of ureC and 16S rRNA genes confirmed the isolate as Photobacterium (P.) damselae subsp. damselae and this pathogen was sensitive to gentamicin. Although, no mortality in mouse was observed in the experimental infection study, the isolation of this pathogen in aquarium fish is significant because it can act as a reservoir to other aquatic animals and can also be zoonotic potential to human during aquarium management. This paper describes the first isolation of P. damselae subsp. damselae from zebra shark.
HAN, Jee Eun,GOMEZ, Dennis K.,KIM, Ji Hyung,CHORESCA JR., Casiano H.,SHIN, Sang Phil,PARK, Se Chang Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.1
<P>The Egyptian fruit-bat <I>Rousettus aegyptiacus</I> which had been raised at the private commercial aquarium in Seoul, Korea for indoor exhibition was found dead and submitted to College of Veterinary Medicine, Seoul National University for postmortem examination. A pure bacterium of <I>Kluyvera ascorbata</I> was isolated from the blood specimen. The isolation of <I>K. ascorbata</I> from fruit bat is very important, because it is the most infectious agent of the genus Kluyvera that cause serious diseases to animals and human. Fruit-bats which are distributed in pet shops through black-market in Korea although unproven become popular pet nowadays. This situation enhances chance of zoonosis. This paper describes the first isolation of <I>K. ascorbata</I> from the Egyptian fruit-bat.</P>
Choresca, Casiano H. Jr.,Gomez, Dennis K.,Han, Jee-Eun,Shin, Sang-Phil,Kim, Ji-Hyung,Jun, Jin-Woo,Park, Se-Chang The Korean Society of Veterinary Science 2010 大韓獸醫學會誌 Vol.50 No.4
Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.
Occurrence of Goiter in Flowerhorn Cichlid (Family : Cichlidae) and Its Effect on Liver
신상필,지향,김지형,Dennis K. Gomez,Casiano H. Choresca Jr,한지은,김대용,박세창 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.2
A flowerhorn fish with a subopercular mass, which had been reared in one of the private commercial aquaria was sent to the laboratory just before death. This fish began to show symptoms such as lethargy, anorexia, depression and dyspnea, and eventually died. The mass and intestinal organs were examined histopathologically. Thyroid showed variably sized follicles that contained abundant colloid and liver revealed steatosis. This report presents a case of goiter in the flowerhorn cichlid and its effect on the liver.
Fate and Survivability of Fish Bacteriophage Inoculated in BALB/c Mice
Ji Hyung Kim,Kyung Taeck Lim,Dennis K. Gomez,Gun Wook Baeck,Gang Joon Heo,Se Chang Park 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.4
Understanding of bacteriophage kinetics in mammals or higher organisms is needed, as this determines the potential phage activity in antibacterial treatment. We demonstrated the fate and survivability of fish phage particles in different organs and fluids inoculated into mice. Phage (PPpW-4) specific to Pseudomonas plecoglossicida was intraperitoneally injected to mice with a dose of 10<SUP>8.1</SUP> PFU/㎖/mouse. Phage titers (10<SUP>3.0</SUP>~10<SUP>5.2</SUP> PFU/g) were detected in blood and other organs within 10 min after injection except in the urine samples. Phage titers in blood (10<SUP>5.1</SUP>~10<SUP>6.9</SUP> PFU/g) were 10~100 times higher than that of the other organs from 10 min until 24 h. It shows that phage was distributed quickly to the different organs by circulatory system via the blood. Phage titers in all organs showed the highest level in 1 h (10<SUP>4.2</SUP>~10<SUP>6.9</SUP> PFU/g) and reduced rapidly until no phage was detected in 72 h. Although phage disappeared in blood within 72 h, phage can still survived in the spleen and kidney until 96 h. Spleen in particular, had an appreciable phage titer of 10<SUP>2.7</SUP> PFU/g. There were comparatively high phage titers (10<SUP>3.0</SUP>~10<SUP>4.7</SUP> PFU/g) in urine from 1 h up to 24 h. It means that one possible mechanism of phage elimination is by urinary excretion.