김대건 (안드레아) 신부 얼굴복원

한승호(Seung-Ho Han),박대균(Dae-Kyoon Park),이우영(U-Young Lee),고기석(Ki-Seok Kohl),김희진(Hee-Jin Kim),허경석(Kyung-Seok Hu),남용석(Yong-Seok Nam) 대한체질인류학회 2001 대한체질인류학회지 Vol.14 No.3

본 작업은 서울대교구 명동성당에서 가툴릭의대에 우리나라 최초의 신부인 김대건 신부의 얼굴복원작 업을 의뢰해 1999년 9월부터 약15개월에 걸쳐 북원작업을 한 것으로 1971년 가툴릭의대 해부학교실에서 조사한 김대건 신부 머리뼈의 직접제측치와 사진 자료를 창조하여 제작하였다 작업의 과정은 머리뼈 계측값이 가장 비슷한 머리뼈를 찾아 복제를 하고 계측치에 맞게 수정한 후, 해부학 실습용 시체에서 직접계측하거나 초음파를 이용하여 간접계측을 한 한국인 얼굴의 표준두께를 적용하여 영굴을 복원하였다. 아울러 한국인에서 조사 한 머리뼈와 얼굴 각 부위의 연관관계자료를 토대로 유토를 이용한 복원작업을 시행하였으며 최종 결과물은 청동을 소재로 흉상을 만들었다. 이와 같은 작업은 법의학분야에서 신원미확인 머리뼈가 발견되었을 때 얼굴복원을 통해 신원을 확인하는 법 의인류학적 연구의 하나로, 한국인의 자료를 토대로 작업한 최초의 영굴복원이며 앞으로 신원확인을 위해 얼굴 을 복원하는 작업에 도움이 될 것으로 생각한다.

신호교차점 접근로의 지체해석에 관한 연구

우용한,김대웅,최용석 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

This study was a selection circular road of Taegu city and four intersections approach which is located in the suburbs as the subject of study. And then, observed the relation between volume, traffic, speed and distribution of vehicle speed. It was examined the goodness of fit after making a model through the decelerated delay, accelerated delay and stopped delay which are calculated on the basis of the survey data. And also looked into the relation among the approach delay and the stopped delay and vehicles delay of signalized intersection approach which was caused by the traffic congestion. In the comparison of the average stopped delay for the model, KHCM model have an observation values and an approximate value. It was known the fact that the prediction model (1) and (2) that is provided by this study can applied at present. It could use this result to the shortest route search method and the signal system operation, the calculation of the service level at the signalized intersections.

대한 응급의학회지 논문의 분석 및 고찰

손대곤,장석준,송근정,이한식 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

To raise the quality of research, medical articles which are feasible and objective must be continuously published and be continuously monitored. All articles published in the Journal of the Korean Society of Emergency from November, 1990 to November, 1995 were compared with a foreign emergency medical journal, the American Journal of Emergency Medicine(AJE). Classifying the articles in the Journal of the Korean Society of Emergency Medicine, there were 15 (8.4%) review articles, 145 (81.0%) original articles and 19 (10.6%) case reports. In the AJE, there were 103 (15.1%) review articles, 304 (44.4%) original articles and 277 (40.5%) case reports. Classifying the original articles in the Journal of the Korean Society of Emergency Medicine, There were 13 (9.0%) experimental articles, 88 (60.7%) descriptive articles and 44 (30.3%) analytic articles. In the AJE, there were 40 (13.2%) experimental articles, 59 (19.4%) descriptive articles and 205 (67.4%) analytic articles. The classification of original articles according to frequency in the Journal of the Korean Society of Emergency Medicine is trauma, resuscitation, toxicology and medical emergency, where as the AJE is medical emergency, trauma, toxicology and EMS. For statistical methods used in original articles, 90 (62.1%) case did not do any statistical analysis or where only descriptive statistics were used. In 6 (4.1%) cases the statistical level was described but statistical methods were nonmentioned. By simple calssificaton study, there were 44 (30.3%) cases of original articles in the Journal of the Korean Society of Emergency Medicine, and 6 (2.0%) cases in the AJE. Because the development of Emergency Medicine as a special department is based on logical articles, organization of articles have study design, make hypothesis, sample gathering, statistical analysis and feasibility of conclusion. For this, the emergency training course needs a course on research methodology.

어성초 헥산 추출물이 암세포주에 미치는 항암효과

서대호,한두석 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

In order to develop cancer agents which indicate weak side effects and strong cancer activity, cytotoxicity of Houttuynia cordata extracts against human oral epithelioid carcinoma cells (KB) and human skin melanoma cells (SK-MEL-3) was evaluated by MTT assay. KB and SK-MEL-3 cells were cultured in RPMI 1640 media containg 10% fetal bovine serum, antibiotics and fungizone. After incubation for 24 hours, the cells were treated with Houttuyunia cordata extracts by dose dependent manner for 48 hours under the same condition. Also, SRB assay for quantity of nucleaic protein, PAS reaction for glycoprotein in cytoplasm and XTT assay for extracellular laminin have done by Elisa reader (spectra Max 250, USA) and PAS stain, respectively. The microscopic study was carried out to observe morphological changes and PAS positive reactions in KB and SK-MEL-3. The results were as follows ; 1. The MTT absorbances against KB and SK-MEL-3 in 10^-2 ㎎/㎖ of hexane extracts were significantly decreased to 77.6% and 78.4%, respectively. 2. The SRB absorbance against KB and SK-MEL-3 in 10^-2 ㎎/㎖ of hexane extracts were significantly decreased to 79.2% and 79.5%, respectively. 3. In 10^-2 ㎎/㎖ of hexane extract, PAS reaction of KB cells was changed to negative and PAS positive cells of SK-MEL-3 were decrease to below 30%. 4. The XTT absorbances against KB and SK-MEL-3 in 10^-2 ㎎/㎖ of hexane extract were significantly decreased to 76.1% and 78.0%, respectively. 5. The morphological change were observed and number of cells were decreased against the cells in 10^-2 ㎎/㎖ of hexane extract. These results suggest that hexane extract of Houttuynia cordata has shown inhibitory effects in the proliferation, differentiation and metastasis against KB and SK-MEL-3.

동일한 혈관 내에 발생한 이식형 약제 주입기 카테터의 위치이상

오대석,김영재,김명훈,조광래,임세훈,이정한,이근무,정순호,신치만 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

저자들은 56세 여성 환자의 왼쪽 쇄골 하 정맥에 시행한 이식형 약제주입기 카테터의 삽입 후 발생한 비정상 위치에 대한 증례를 보고하고자 한다. 카테터의 끝은 환자의 늘 어진 가슴, 체위, 신체의 움직임, 카테터의 유동성 등의 영향으로 천자된 정맥 내에서 견인되고 뒤쪽으로 이동하였다. 환자는 어떠한 증상도 호소하지 않았지만 비정상 위치의 카테터는 심각한 합병증을 유발할 수 있기 때문에 제거되었다. 따라서 시술자는 약제주입기 삽입 전에 환자의 신체 상태와 단순 흉부 촬영상을 확인하고 카테터의 비정상 위치가 발생할 수 있는 위험 징후를 알아차려야 하겠다.

국산 DAF 펌프의 기포특성

안대명,이창한,최윤찬,조석호,안갑환,김성수 대한상하수도학회 2002 상하수도학회지 Vol.16 No.4

The paper introduces a domestic pump designed for dissolved air flotation (DAF). In the paper, the difference in performance between the domestic DAF pump and conventional pressure saturator is explained using the characteristics of air bubble released. The size distribution of air bubbles released from DAF pump is measured by image analyzer and particle size analyzer. And the mean diameter of bubbles is 43μm. The results are similar to that of pressure saturator. The air volume concentration in the water of domestic DAF pump and pressure saturator are 40 and 70% of theoretical amounts. In DAF system used domestic DAF pump, the removal efficiency of turbidity is 70%.

敗醬草가 癌細胞柱에 미치는 影響

申大澈,權貞南,金瑩均,韓宗鉉,김재석,김재섭 동의대학교 한의학연구소 1997 동의한의연구 Vol.1 No.-

The purpose of this study was to investigate effect of water extract of Patriniae Herba on the poliferation of human cancer cell-lines. The effects of Patriniae Herba on the poliferation of A43l, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay, The results were as follows; 1. Patriniae Herbs did not effect A43l, HeLa, MOLT-4, K562 cells. 2. The cytotoxicity of mitomycin C on MOLT-4 cells was increased by the combination of Patriniae Herba. 3. Patriniae Herba inhibited the proliferation of Balb/c 3T3 cells. 4. Patriniae Herba stimulated the proliferation of thymocytes. 5. Patriniae Herba stimulated the proliferation of splenocytes. 6. Patriniae Herba stimulated the proliferation of human lymphocytes.

버섯 배지를 이용한 tyrosinase 저해제 발효

정승원,한대석,김석중,전문진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

Tyrosinase는 식품의 효소적 갈변과 생체내에서 melanin 합성을 촉매하는 효소이다. 식용 가능한 tyrosinase 저해제의 발효생산을 통하여 melanin 합성을 저해하는 효과를 가지는 미백식품 소재를 개발하고자 현재 식품의 제조에 이용되고 있는 안전한 미생물을 대상으로 tyrosinase 저해능이 높은 균주를 선발하고 표고버섯을 주 배지원으로 발효시켜 저해제 생산의 적정조건에 대하여 연구하였다. 발효식품으로부터 분리된 NU-7을 tyrosinase 저해제 생산을 위한 발효균으로 선정하여 tyrosinase 저해제 생산 최적배지 조건을 확인한 결과 glucose가 3~20% 및 ammonium sulfate가 0~0.25%인 범위에서 균체량 증가에 따른 tyrosinase 저해제 생산능의 차이는 나타나지 않았으며, 무기질소원으로 첨가한 ammonium sulfate의 영향은 비교적 낮았다. 한편, 표고버섯(Lentinus edodes, Shiitake) 함량은 저해제 생산과 밀접한 관계를 나타내어 버섯함량(X)에 대한 저해제 생산량(Y)이 Y=-0.96X^2+13.07X+14.43의 상관식을 보였고, R value는 0.96으로 나타나 최적발효 조건을 위한 최소한의 배지는 표고버섯과 glucose 만으로도 가능한 것으로 판단되었다. 표고버섯을 이용한 발효액에서 곰팡이 독소로 알려진 아플라톡신은 검출되지 않았으며 따라서 이를 식용할 수 있는 가능성이 높은 것으로 판단되었다. Tyrosinase is an enzyme which catalyzes an enzymatic browning of some foods and in vivo synthesis of melanin. In order to produce natural and edible inhibitor of the enzyme which is expected to have whitening effect on melanogenesis, a microorganism was selected from fermented foods. It was named as NU-7, and cultured in mushroom (Lentinus edodes, Shiitake) media. Optimal media to produce tyrosinase inhibitor was formulated by varing nitrogen or carbon content. If glucose content was in a range of 3~20% and ammonium sulfate was in a range of 0~0.25%, production of inhibitor was independent of cell mass. Addition of ammonium sulfate as a nitrogen source had little effect on inhibitor production. Production of inhibitor (Y) was proportionally related to shiitake content (X) with a regression equation of Y=-0.96X^2+13.07X+14.43 (R=0.96). These results indicate that shiitake and glucose are necessary for the production of tyrosinase inhibitor. In the analysis of mycotoxin in culture broth, aflatoxin was not detected, suggesting that it would be probably edible.

신경망 이론을 이용한 국도구간의 통행시간 추정모델

도명식,한대석,유영근 한밭대학교 2004 한밭대학교 논문집 Vol.21 No.-

Travel time estimation has been recognized as one of the most critical elements of Intelligent Transportation Systems. This paper presents a method for estimating link travel time using neural network method. For estimation of travel time, traffic volume and mean velocity data from detectors are used. Also, In order to measure travel time by the time difference at each link or sectional data, license plate matching techniques are adopted. Target section is determined on a part of National road number 3, which is pilot section of NHTMS(National Highway Traffic Management System). It runs 10.72km from Gonjiam IC to Jang-ji IC. Neural network based travel time estimation model has been developed and tested using different data sets such as each link-based traffic data set and homogeneous section based data set. The optimal configuration of the neural network architecture is determined by self organization method. In conclusion, we know that the model by using all road section data is more efficient.

PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.