Effect of Developmental Lead Exposure on the Expression of Hippocampal NMDA Receptor Subunit mRNA

Kim, Tae-Wan,Chung, In-Sung,Bae, Jae-Hoon,Shin, Dong-Hoon,Lee, Mi-Young,Kim, Joon-Sik 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

목적: in vivo 및 vitro에서 해마 신경세포의 발생단계별 NMDA 수용체 아단위 mRNA 발현에 대한 연 폭로 영향을 알아보고자 하였다. 방법: 흰쥐 해마 신경세포의 발생단계별 NMDA 수용체 NR2A, NR2B 아단위 mRNA 발현에 대한 연의 영향은 정상군과 연 폭로군의 출생 후 7일, 14일, 22일 흰쥐의 해마에서 in situ hybridization으로 mRNA 발현 정도를 densitometer로 측정하여 비교하였고, 연과 NMDA 의 세포독성은 해마 신경세포 일차배양 후 도립현미경을 이용한 형태학적인 관찰과 LDH 활성도를 이용하여 측정하였다. 결과: 연 과 NMDA 에 의한 세포독성에 대한 in vitro 실험에서 형태학적 소견과 LDH 활성도에서 해마 미성숙 신경세포와 성숙 신경세포사이의 차이가 있었으므로, 연과 NMDA 독성효과는 해마 신경세포의 발달 단계에 따라 차이가 있다. 정상군의 해마에서의 NR2A mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나, NR2B mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나 NR2B mRNA 발현은 연령의 증가에 따른 변화가 없었다. 연 폭로에 희한 NR2A mRNA 발현은 유의하게 감소하였으나(p<0.05), NR2B mRNA 발현은 변화가 나타나지 않았다. 만성적 연 폭로는 NR2A를 포함하는 NMDA 수용체를 감소시킬 수 있음을 알 수 있다. 결론: 연은 해마신경세포의 발생단계에서 NMDA 수용체 아단위 특히 NR2A mRNA 발현의 변화를 야기하여 시냅스 신호 전달에 영향을 나타냄을 알 수 있었다. Hippocampus

T 및 B cell 測定 : 1. 1次 成績 Ⅰ. Results, Primary

金在植,崔成萬,金在龍,全東錫,朴正姬,徐相喆,金仁子,金在崇,金重明 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

E-rosette法에 의한 T cell의 百分率과 螢光抗體法, 感作牛赤血球擔體法, 酵素抗體法 및 마우스赤血球 rosette法에 의한 B cell 의 百分率을 檢査하여 다음과 같이 요약할 수 있었다. 정상성인군의 active T cell은 26±8.2%이었고 total T cell은 72±4.9%이었다. B cell 는 螢光抗體法으로 22±5.4%, 感作牛赤血球擔體法으로 15.8±4.9%, 酵素抗體法으로 15±5.2% 그리고 mouse RBC-rosette法으로 17.2±4.1%이었다. 惡性腫瘍에 있어서 active T cell은 27.±10.4%이었고 total T cell 은 59±11.2%로서 정상인군에 비하여 유의한 감소를 보였다. 良性疾患에 있어서 T cell 은 정상인군에 비하여 유의한 차이를 볼 수 없었다. T cell percent by E-rosette and B cell by fluorescent antibody, senitized ox RBC rosette, enzyme antibody and mouse RBC rosette tests were performed. In healthy adult group the active T cell was 26±8.2% and total T cell was 72±4.9%, B cell by fluorescent antibody was 22.±5.40%, by ox RBC rosette 15.8±4.9%, by enzyme antibody 15±5.2% and by RBC-rosette 17.2±4.1%. In malignant tumor the active T cell was 27±10.4% and total T cell was 59±11.2%. In benign diseases T cell count showed no significant difference from normal adult group.

가토에 있어서 편측 요관 폐색시 환측, 반대측 요관에 대한 변화 : 조직학적 소견을 중심으로

설종구,김용웅,육승모,노안식,김홍식,김상현,이충식 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

This study was designed to clarify histological changes in the experimental rabbit ureter after unilateral prartial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, partial obstruction followed by 4 weeks, complete obstruction followed by 2 weeks, complete obstruction followed by 4 weeks. The normal control group consisted of 5 adult white rabbits. The H & E stain. light microscopy were used. Abnormal histological fingings were showed in ipsilateral ureter of all subgroups; dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis, interstitial inflammation. These findings were most severe in the complete-obstructed ureter for 4 weeks. There were no significant changes in contralateral ureter of all subgroups. Despite of progressive histological changes in ipsilateral ureter no abnormal findings were showed in contralateral ureter. We suggest that long term experimental investigation for histological changes in ipsilateral ureter and compensatory changes in contralateral ureter should be done.

q진 LCZ 수열군의 일반화된 확장 생성 방법

정정수(Jung-Soo Chung),김영식(Young-Sik Kim),장지웅(Ji-Woong Jang),노종선(Jong-Seon No),정하봉(Ha-Bong Chung) 한국통신학회 2008 韓國通信學會論文誌 Vol.33 No.11C

[1]에서 LCZ 수열군의 2배 확장을 제안하였다. 본 논문에서는 [1]에서의 2배 확장방법을 일반화하는 새로운 확장방법을 제안한다. 이 생성방법을 사용하면 인수가 (N,M,L,ε)인 q진 LCZ 수열군은 인수가(pN,pM,p「(L+1)/p」-,pε)인 q진 LCZ 수열군이 된다. 이 때, p는 소수이고 p는 q의 약수다. 특히 L≡p-1modp 일 때, 확장된 q진 LCZ 수열군의 인수는 (pN,pM,L,pε)이 된다. In this paper, a new extending method of q-ary low correlation zone(LCZ) sequence sets is proposed, which is a generalization of binary LCZ sequence set by Kim, Jang, No, and Chung. Using this method, q-ary LCZ sequence set with parameters (N,M,L,ε) is extended as a q-ary LCZ sequence set with parameters (pN,pM,p「(L+1)/p」-1,pε), where p is prime and p|q.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Son, Ji Yeon,Kang, Yoon Jong,Kim, Kyeong Seok,Kim, Tae Hyung,Lim, Sung Kwang,Lim, Hyun Jung,Jeong, Tae Cheon,Choi, Dal Woong,Chung, Kyu Hyuck,Lee, Byung Mu,Kim, Hyung Sik Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Ji Yeon Son,Yoon Jong Kang,Kyeong Seok Kim,Tae Hyung Kim,Sung Kwang Lim,Hyun Jung Lim,Tae Cheon Jeong,Dal Woong Choi,Kyu Hyuck Chung,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

腹腔姙娠의 一例

金忠植 全南大醫大 1968 전남의대학술지 Vol.5 No.1

한국 재원 정신분열병환자의 사회경제적 특성에 관한 역학적 조사 : 일일 시정조사 Point Prevalence Study

정인원,김용식,이정균 大韓神經精神醫學會 1985 신경정신의학 Vol.24 No.1

1984년 2월 27일 현재 전국 정신과 병·의원의 입원환자를 대상으로 일일시정조사를 시행하였다. 자료분석 때까지 도착한 자료중 ICD-9의 진단기준에 의하여 정신질환의 범주에 속하는 2,895명을 1:1 쌍쌍대응법으로 분석하여 거주지별로 정신분열병군과 대조군간의 사회경제적 특성을 비교하였다. 분석결과는 다음과 같았다. 1. 정신분열병환자의 결혼상태는 두 지역 모두에서 독신이 많았고 odd ratio는 각각 5.33과 3.56이었다. 농ㆍ어촌의 정신분열병환자가 독신이 더 많았다. 2. 교육수준은 양 군에서 뚜렷한 차이는 없었다. 3. 직업은 도시의 정신분열병환자에서 무직이 많았고 odd ratio는 1.90이었다. 4. 경제수준은 도시의 정신분열병환자에서 낮은 층이 많았고 odd ratio는 1.65이었다. 5. 그외 가족구조와 종교, 부모의 결혼상태에서는 유의한 차이를 보이지 않았다. Aiming at suggestion the possible ways to improve psychiatric care, rehabilitation, and mental health services in Korea, this nation-wide hospital study was intended to explore the socioeconomical status of mental in-patients. This point prevalence study was conducted to patients who were in psychiatric wards at Feb. 27th. 1984 using mailing questionnaire. The number of hospitals and clinics answered was 88 and the patients, 3,300. Among them, we selected the 2,895 patients who were included in mental disorders (290-319) by ICD-9. Finally we compared the socioeconomical status between schizophrenia and non-schizophrenia in rural and urban area with the method of Fleiss. The results were as follows; 1. The relative proportions of single persons in schizophrenia were higher in both areas, and their odds ratios were 5.33 and 3.56, respectively. There were more single schizophrenics in rural than urban. 2. In educational levels, there were no significant differences between schizophrenia and control. 3. In urban area, the jobless persons were more in schizophrenia than control, and its odd ratio was 1.90. 4. The urban schizophrenics were significantly lower than the urban control in economical levels, and its odd ratio was 1.65. 5. About family structure, religion, and marital status of parents, there were no significant differences between schizophrenia and control.

자라의 腎臟機能에 미치는 Oxytocin 및 Vasopressin의 影響

金忠植 全南大醫大 1968 전남의대학술지 Vol.5 No.1

體操競技 男·女 種目別 着地 成功率에 關한 比較分析

金忠泰,韓忠湜 韓國體育大學校 1993 論文集 Vol.16 No.-

The 47 players(M : 23, F : 24) who participated the gymnastic games(The 3rd game : the finals in each events) of the 11th Bei-jing Asian games were made an object of this research. The success rate of landing performance substances of 3 events(M : vaulting horse, parallel bars, horizontal bar. F : vaulting horse, balance heam, uneven bars) wich are similar in performance substance and technical figures. As a result rate of researche and analysis, the conclusion is as follows. 1. The success rate of landing in performance substance. 1) Vaulting horse(M,F) Men : yurenchenko technic was practiced 7 times at the first time and second time and it was proved as the highest success rate. The highest was 100% and the lowest was 60%. Handspring technic was practiced 2 times(at the first time and the second time) and it was proved as the lowest success rate. The highest 40% and the lowest was 0%. Women : Tsukahara technic was paracticed 3 times at the first time and second time and it was proved as the highest success rate. The highest was 80% and the lowest was 60%. Handspring technic was practiced 6 times(at the first time and second time) and it was proved as the lowest success rate. The highest was 80% and the lowest was 0%. 2) Parallel bars(M), Balance beam Parallel bars(M) : Double salto bwd. tucked, Double salto bwd. piked technics were proved as the highest success rate of 100%, and From hang on end double salto bwd. piked technic was proved as the lowest success rate of 60%. Balance beam(F) : Double salto bwd. tucked technic was proved as the highest success rate of 100% and Double salto bwd. tucked w. 1/1 twist technic was proved as the lowest success rate of 60%. 3) Horizontal bar(M), Uneven bars(F) Horizontal bar(M) : Double salto bwd. str. w. 2/1 turns technic was proved as the highest success rate of 100% and Triple salto bwd. tucked technic was proved as the lowest success rate of 60%. Uneven bars(F) : Double salto bwd. tucked technic was proved as the highest success rate of 100% and Double salto bwd. tucked w. 1/1 twist technic was proved as the lowest success rate of 60%. 2. The success rate of landing in technical figures. 1) Vaulting horse(M,F) Men : Stretched forms of technic which was practiced 8 times and 4 times at the first time and the second time was proved as the highest success rate of 71%. Piked forms of technic which was practiced once at the first time and the second time was proved as the lowest success rate of 40%. Women : Stretched forms of tetchnic which was practiced 6 times and once at the first time and the second time was proved as the highest success rate of 86%. Tucked forms of technic which was practiced once and 4 times at the first time and the second time was proved as the lowest success rate of 52.5%. 2) Parallel bars(M), Balance beam(F) Parallel bars(M) : Picked forms of technic which parcticed 3 times was proved as the highest success rate of 86% and Tucked forms of technic which parcticed 5 times was proved as the lowest success rate of 84%. Balance Beam(F) : Tucked forms of technic which parcticed 7 times was proved as the hifhiest success rate of 77% and Piked forms of technic which parcticed once was proved as the lowest success rate of 60% 3) Horizontal bar(M), Uneven bars(F) Horizontal bar(M) : Stretched forms of technic which was practiced 6 times was proved as the highest success rate of 76% and Tucked forme of technic which was practiced once was proved as the lowest success rate of 60%. Uneven bars(F) : All the players practiced Tucked forms of technic and they got the landing success rate of 85%.