Ratchet Effect of Domain Wall Motion by GHz AC Magnetic Field in Asymmetric Sawtooth-Shaped Ferromagnetic Nanowires

Piao, Hong-Guang,Lee, Hana,Yoon, Jungbum,Kim, Dong-Hyun,You, Chun-Yeol,Kim, Tae Wan IEEE 2010 IEEE transactions on magnetics Vol.46 No.6

<P> We have investigated a ratchet effect of magnetic domain wall in asymmetric sawtooth-shaped ferromagnetic nanowires under high frequency AC magnetic fields by means of micromagnetic simulation. The ratchet phenomenon has been intensively examined during the domain wall propagation with variation of the asymmetric sawtooth geometry of nanowires as well as variation of the AC external field frequency/strength. Very interestingly, it has been found that there is a strong correlation between the spatial frequency of asymmetrical notches and the effective driving frequency/strength of AC external fields. It is demonstrated that number of notches swept by domain wall ratchet motion per each half-cycle of the driving frequency is controlled under a GHz AC driving field. </P>

Construction of Optimal Concatenated Zigzag Codes Using Density Evolution

Song-Nam Hong,Yong-Chun Piao,Dong-Joon Shin 한국통신학회 2005 한국통신학회 학술대회 및 강연회 Vol.2005 No.4

차별 발현 역전사중합효소연쇄반응법을 이용한 원발 간세포암종의 유전자 발현 분석

이영춘 ( Young Chun Lee ),허원희 ( Won Hee Hur ),최정은 ( Jung Eun Choi ),박련숙 ( Lian Shu Piao ),홍성우 ( Sung Woo Hong ),배시현 ( Si Hyun Bae ),최종영 ( Jong Young Choi ),윤승규 ( Seung Kew Yoon ) 대한소화기학회 2009 대한소화기학회지 Vol.53 No.6

목적: 원발 간세포암종에서 특이하게 과발현되는 특정유전자를 규명하는 것은 간세포암종 발암 기전의 연구나, 암의 조기진단 및 암 특이 유전자를 표적으로 한 유전자 치료에 매우 중요한 정보를 제공한다. 이에 이번 연구에서는 차별 발현 역전사중합효소연쇄반응법을 활용한 유전자 분석을 통해 간세포암에서 특이하게 과발현되는 특이 유전자를 밝혀내고 이 유전자의 발현율을 여러 시료에서 확인하여 간세포암 관련 유전자들의 기능을 탐색하고자 하였다. 대상 및 방법: 원발 간세포암종으로 수술을 시행 받았던 3명의 간세포암 환자의 간 적출물에서 간세포암종 조직과 암주변의 비 간세포암 조직 3쌍을 채취한 후 차별 발현 역전사중합효소연쇄반응법을 이용하여 간세포암종 조직에서 과발현되거나 소실된 유전자 혹은 저발현되어 있는 유전자를 겔로부터 DNA를 용출하여 이를 클로닝한 후 염기서열분석을 통하여 나타난 유전자의 서열을 유전자 검색프로그램에 넣어 그 유전자의 이름이나 특성들을 탐색하였다. 이후 새로 규명된 유전자의 기능을 검증하기 위하여 11쌍의 간세포암종 및 비간세포암종 조직과 인간 암세포주인 간세포암종 세포주[HepG2, Hep3B, Huh7], 자궁암 세포주[HeLa], 대장암 세포주[HT1299], 섬유육종 세포주[HT1080], 폐암 세포주[A549]에서 실시간 중합효소연쇄반응을 이용하여 이들 유전자의 발현을 확인한 후 분석하였다. 결과: 이번 연구를 통해서 단백 대사, 유비퀴틴 의존성 단백 대사, 탄수화물 대사, 지방대사, 유전자복구, 염증 반응 등의 다양한 기능을 가지는 21종의 유전자들을 탐색하였다. 결론: 이번 연구를 통하여 간세포암종 조직과 비간세포암종 조직에서 발현차이를 보이는 유전자를 탐색하였고, 이러한 유전자의 특성 규명은 간세포암종의 발암 기전을 규명하거나 조기 간세포암을 진단하는 바이오 마커의 개발 혹은 분자표적을 대상으로 하는 유전자 치료의 개발에 기초자료로 유용할 것으로 생각한다. Background/Aims: The investigation of a specific tumor marker for hepatocellular carcinoma (HCC) is needed to examine the carcinogenesis and to select the patients for treatment options. The aim of this study was to find the genes related to HCC. We also examined the expression level of these genes in cancer cell lines and tissue specimens. Methods: Three pairs of HCC tissue and non-neoplastic hepatic tissue around the HCC were collected from three patients who underwent resection for HCC. Differential display reverse transcriptase-PCR (DD RT-PCR) using GeneFishing(TM) PCR was used to detect the differences in the gene expression between in HCC tissue and non-neoplatic tissue. Up- or down-regulated genes in HCC tissue were identified through BLAST searches after cloning and sequencing assays. Real-time RT-PCR assay was employed to detect the expression rate in 11 HCC tissues and human cancer cell lines. Results: Differentially expressed 21 genes were identified, and they were classified as genes involved in protein metabolism, ubiquitin-dependent protein catabolism, carbohydrate metabolism, lipid metabolism, DNA repair, and inflammatory response. Conclusions: We identified differentially expressed genes in HCC, and these genes may play an important role in the study of hepatocarcino-genesis, development of biomarker, and target therapy for HCC. (Korean J Gastroenterol 2009;53:361-368)

Analysis of Gene Expression in Primary Hepatocellular Carcinoma Using Differentially Displayed Reverse Transcriptase Polymerase Chain Reaction

Lee, Young Chun,Hur, Wonhee,Choi, Jung Eun,Piao, Lian Shu,Hong, Sung Woo,Bae, Si Hyun,Choi, Jong Young,Yoon, Seung Kew The Korean Society of Gastroenterology 2009 대한소화기학회지 Vol.53 No.6

<P>BACKGROUND/AIMS: The investigation of a specific tumor marker for hepatocellular carcinoma (HCC) is needed to examine the carcinogenesis and to select the patients for treatment options. The aim of this study was to find the genes related to HCC. We also examined the expression level of these genes in cancer cell lines and tissue specimens. METHODS: Three pairs of HCC tissue and non-neoplastic hepatic tissue around the HCC were collected from three patients who underwent resection for HCC. Differential display reverse transcriptase-PCR (DD RT-PCR) using GeneFishingTM PCR was used to detect the differences in the gene expression between in HCC tissue and non-neoplatic tissue. Up- or down-regulated genes in HCC tissue were identified through BLAST searches after cloning and sequencing assays. Real-time RT-PCR assay was employed to detect the expression rate in 11 HCC tissues and human cancer cell lines. RESULTS: Differentially expressed 21 genes were identified, and they were classified as genes involved in protein metabolism, ubiquitin-dependent protein catabolism, carbohydrate metabolism, lipid metabolism, DNA repair, and inflammatory response. CONCLUSIONS: We identified differentially expressed genes in HCC, and these genes may play an important role in the study of hepatocarcinogenesis, development of biomarker, and target therapy for HCC.</P>

Gastrin 유발 위점막 손상에 대한 Nicotine의 보호 효과

박세호(Shi Hao Piao),김동구(Dong Goo Kim),김덕남(De Nan Jin),오정구(Zhen Jiu Wu),홍춘란(Chun Lan Hong),김경환(Kyung Hwan Kim) 대한약리학회 1995 대한약리학잡지 Vol.31 No.3

Conflicting data have been reported on the effect of nicotine on gastric mucosal damage. To elucidate the effect of chronic intermittent nicotine on gastric mucosal damage, intragastric nicotine (5 mg/kg, 10 mg/kg) was administered twice per day for 9 days. Gastric mucosal damage was created by s.c. injection of a large dose (1.2 mg/kg) of pentagastrin followed by pylorus ligation for 6 hours. Nicotine treated rats showed reduced gastric mucosal damage about 50% of the control. To examine the mechanism of the protective effect of nicotine, gastric perfusion experiments were done. Basal acid secretion was not affected by intragastric or intravenous nicotine. However, pentagastrin-stimulated acid secretion markedly inhibited by a bolus injection of nicotine, and this response was dose-related. These data indicates that chronic intermittent administration of nicotine protects gastric mucosa against gastrin-induced gastric mucosal damage, and nicotine-induced inhibition of gastrin-stimulated acid secretion has an important role for the protective effect of nicotine. Considering reports concerning nicotine s aggravating effect on the gastric mucosal damage, it is suggested that the methods of administration of nicotine may be an important decisive factor of the divergent action of nicotine on the gastric mucosa. 위점막 손상에 미치는 영향에 관한 nicotine의 효과는 아직 정설이 없는 형편이다. 본 연구에서는 nicotine이 위점막 손상에 미치는 영향을 보기 위하여 nicotine (5 mg/kg, 10mg/kg)을 9일간 하루에 두번씩 위내 투여하였다. 위점막 손상은 gastrin (1.2 mg/kg)을 피하 주사함과 동시에 유문부결찰을 6시간 동안 시행하므로 야기시켰다. 그 결과 nicotine 투여군에서 현저한 위점막 손상의 감소를 보였다 (대조군의 50%). 이러한 nicotine의 위점막 보호 효과에 대한 기전을 추구하기 위하여 위관류 실험을 시행하였다. Nicotine은 기초 위산 분비에는 영향이 없었으나 gastrin으로 자극된 위산 분비를 현저히 감소시켰고, 이러한 반응은 nicotine 용량에 비례하였다. 이상의 결과로 보아 nicotine의 장기간 간헐적 투여는 gastrin 투여로 인한 위점막 손상에 보호 효과가 있으며, 이러한 효과는 gastrin으로 자극된 위산 분비를 억압하는 nicotine의 효과가 관련될 것으로 생각된다. 또한 nicotine의 위점막 손상 악화 효과를 관찰한 보고들을 고려하면 nicotine의 위점막 손상에 관한 효과는 nicotine의 투여 방법에 따라 전혀 다르게 나타날 수 있을 것으로 생각된다.

Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding.

Yang, Keum-Jin,Shin, Sanghee,Piao, Longzhen,Shin, Eulsoon,Li, Yuwen,Park, Kyeong Ah,Byun, Hee Sun,Won, Minho,Hong, Janghee,Kweon, Gi Ryang,Hur, Gang Min,Seok, Jeong Ho,Chun, Taehoon,Brazil, Derek P,He American Society for Biochemistry and Molecular Bi 2008 The Journal of biological chemistry Vol.283 No.3

<P>3-Phosphoinositide-dependent protein kinase-1 (PDK1) appears to play a central regulatory role in many cell signalings between phosphoinositide-3 kinase and various intracellular serine/threonine kinases. In resting cells, PDK1 is known to be constitutively active and is further activated by tyrosine phosphorylation (Tyr(9) and Tyr(373/376)) following the treatment of the cell with insulin or pervanadate. However, little is known about the mechanisms for this additional activation of PDK1. Here, we report that the SH2 domain of Src, Crk, and GAP recognized tyrosine-phosphorylated PDK1 in vitro. Destabilization of PDK1 induced by geldanamycin (a Hsp90 inhibitor) was partially blocked in HEK 293 cells expressing PDK1-Y9F. Co-expression of Hsp90 enhanced PDK1-Src complex formation and led to further increased PDK1 activity toward PKB and SGK. Immunohistochemical analysis with anti-phospho-Tyr(9) antibodies showed that the level of Tyr(9) phosphorylation was markedly increased in tumor samples compared with normal. Taken together, these data suggest that phosphorylation of PDK1 on Tyr(9), distinct from Tyr(373/376), is important for PDK1/Src complex formation, leading to PDK1 activation. Furthermore, Tyr(9) phosphorylation is critical for the stabilization of both PDK1 and the PDK1/Src complex via Hsp90-mediated protection of PDK1 degradation.</P>

Development of the Korea-Polyenvironmental Risk Score for Psychosis

Jeon Eun-Jin,Kang Shi-Hyun,Yan-Hong Piao,Kim Sung-Wan,Kim Jung-Jin,Lee Bong-Ju,Yu Je-Chun,Lee Kyu-Young,Won Seunghee,Lee Seung-Hwan,Kim Seung-Hyun,Kim Eui-Tae,Clara Tammy Kim,Dominic Oliver,Paolo Fusa 대한신경정신의학회 2022 PSYCHIATRY INVESTIGATION Vol.19 No.3

Objective Comprehensive understanding of polyenvironmental risk factors for the development of psychosis is important. Based on a review of related evidence, we developed the Korea Polyenvironmental Risk Score (K-PERS) for psychosis. We investigated whether the K-PERS can differentiate patients with schizophrenia spectrum disorders (SSDs) from healthy controls (HCs).Methods We reviewed existing tools for measuring polyenvironmental risk factors for psychosis, including the Maudsley Environmental Risk Score (ERS), polyenviromic risk score (PERS), and Psychosis Polyrisk Score (PPS). Using odds ratios and relative risks for Western studies and the “population proportion” (PP) of risk factors for Korean data, we developed the K-PERS, and compared the scores thereon between patients with SSDs and HCs. In addition, correlation was performed between the K-PERS and Positive and Negative Syndrome Scale (PANSS).Results We first constructed the “K-PERS-I,” comprising five factors based on the PPS, and then the “K-PERS-II” comprising six factors based on the ERS. The instruments accurately predicted participants’ status (case vs. control). In addition, the K-PERS-I and -II scores exhibited significant negative correlations with the negative symptom factor score of the PANSS.Conclusion The K-PERS is the first comprehensive tool developed based on PP data obtained from Korean studies that measures polyenvironmental risk factors for psychosis. Using pilot data, the K-PERS predicted patient status (SSD vs. HC). Further research is warranted to examine the relationship of K-PERS scores with clinical outcomes of psychosis and schizophrenia.

Distribution and using of Medicinal Plant in Mt. Jangback (Baekdu)

Hu Lin Li(이호림),Ru Zi Piao,Di Liu,Jae Han Cho(조재한),Gi Hong An(안기홍),Won Sik Kong(공원식),Yun Ji Lee(이윤지),Jin Tae Jeong(정진태),Chun Geun Park(박춘근),Jeong Hoon Lee(이정훈),한재구 한국약용작물학회 2020 한국약용작물학회 학술대회논문집 Vol.2020 No.1

Protective Effect of Nicotine on Gastrin-induced Gastric Mucosal Damage in Rats

박세호,김동구,김덕남,오정구,홍춘란,김경환,Piao, Shi-Hao,Kim, Dong-Goo,Jin, De-Nan,Wu, Zhen-Jiu,Hong, Chun-Lan,Kim, Kyung-Hwan The Korean Society of Pharmacology 1995 대한약리학잡지 Vol.31 No.3

위점막 손상에 미치는 영향에 관한 nicotine의 효과는 아직 정설이 없는 형편이다. 본 연구에서는 nicotine이 위점막 손상에 미치는 영향을 보기 위하여 nicotine (5 mg/kg, 10mg/kg)을 9일간 하루에 두번씩 위내 투여하였다. 위점막 손상은 gastrin (1.2 mg/kg)을 피하 주사함과 동시에 유문부결찰을 6시간 동안 시행하므로 야기시켰다. 그 결과 nicotine 투여군에서 현저한 위점막 손상의 감소를 보였다 (대조군의 50%). 이러한 nicotine의 위점막 보호 효과에 대한 기전을 추구하기 위하여 위관류 실험을 시행하였다. Nicotine은 기초 위산 분비에는 영향이 없었으나 gastrin으로 자극된 위산 분비를 현저히 감소시켰고, 이러한 반응은 nicotine 용량에 비례하였다. 이상의 결과로 보아 nicotine의 장기간 간헐적 투여는 gastrin 투여로 인한 위점막 손상에 보호 효과가 있으며, 이러한 효과는 gastrin으로 자극된 위산 분비를 억압하는 nicotine의 효과가 관련될 것으로 생각된다. 또한 nicotine의 위점막 손상 악화 효과를 관찰한 보고들을 고려하면 nicotine의 위점막 손상에 관한 효과는 nicotine의 투여 방법에 따라 전혀 다르게 나타날 수 있을 것으로 생각된다. Conflicting data have been reported on the effect of nicotine on gastric mucosal damage. To elucidate the effect of chronic intermittent nicotine on gastric mucosal damage, intragastric nicotine (5 mg/kg, 10 mg/kg) was administered twice per day for 9 days. Gastric mucosal damage was created by s.c. injection of a large dose (1.2 mg/kg) of pentagastrin followed by pylorus ligation for 6 hours. Nicotine treated rats showed reduced gastric mucosal damage about 50% of the control. To examine the mechanism of the protective effect of nicotine, gastric perfusion experiments were done. Basal acid secretion was not affected by intragastric or intravenous nicotine. However, pentagastrin-stimulated acid secretion markedly inhibited by a bolus injection of nicotine, and this response was dose-related. These data indicates that chronic intermittent administration of nicotine protects gastric mucosa against gastrin-induced gastric mucosal damage, and nicotine-induced inhibition of gastrin-stimulated acid secretion has an important role for the protective effect of nicotine. Considering reports concerning nicotine's aggravating effect on the gastric mucosal damage, it is suggested that the methods of administration of nicotine may be an important decisive factor of the divergent action of nicotine on the gastric mucosa.

Characteristics of lipoxygenase-based and lipoxygenase-deficient soy yogurt with modified okara

Xiujuan Wang,Yue Chen,Yuhua Wang,Weichang Dai,Chun Hong Piao,Hansong Yu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.13

Lipoxygenase-based and lipoxygenase-deficientokara were modified by Kluyveromyces marxianus fermentation,then adding modified okara back to the correspondingsoymilk to prepare soy yogurt. Thephysicochemical properties, texture, and volatile componentsof soy yogurt were characterized. The results showedthat okara modified by Kluyveromyces marxianus fermentationwas rich in soluable dietary fiber and was impartedbetter water-holding capacity, swelling capacity, and oilholdingcapacity. The soy yogurt with the modified okarawas greatly enhanced in its appearance, texture and wasrelatively stable during storage. Moreover, lipoxygenasebasedsoy yogurt had a unique soybean flavor whilelipoxygenase-deficient soy yogurt had a slight beany flavorand soybean flavor. This article guides a bio-modifiedmethod for okara and provides a theoretical basis for thefurther development and application of soy yogurt withhigh dietary fiber as well as lipoxygenase-deficient soyyogurt.