Genome-wide identification and expression pattern of short-wavelength light responsive members of the NAC family in turnip

Wang Jing,Wang Yu,Kong Chang,Liang Yan,Song Wankun,Li Yuhua 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.4

The NAC transcription factor family plays a crucial role in stress response, plant development, and anthocyanin pigmentation. In turnip, anthocyanin biosynthesis is induced by UV-A or blue + UV-B light. However, characterization of turnip NACs is lacking, and whether they are involved in light-dependent anthocyanin accumulation is still unknown. In this study, we identified 200 BrNAC transcription factors in the turnip genome. They were intensively distributed on chromosomes A03, A07, A09, and A10. Phylogenetic analysis classified the turnip BrNAC gene family into 11 subfamilies. The protein subdomains were highly conserved between turnip and Arabidopsis thaliana, and exhibited a close evolutionary relationship. Protein-protein interaction prediction and RNA-seq expression profiles illustrated that the UV-A light responding genes BrNAC183, BrNAC195, and the blue + UV-B light responding gene BrNAC184 were the candidate genes associated with light-dependent anthocyanin accumulation in turnip. We discussed the findings on the relationship between the structure and function of the short-wavelength light responsive BrNAC family proteins from our results and the published data. Our results will aid further functional analysis of BrNAC family transcription factors. This research supports that BrNAC transcription factors regulate light-dependent anthocyanin biosynthesis in turnip.

Ultimate Analysis of Steel Structures Based on Fiber Hinge Model and Time-Varying Structure Theory

Yuhua Wang,Qi Wang,Jun Dong,Yang Peng 한국강구조학회 2015 International Journal of Steel Structures Vol.15 No.3

Oversize deformations and local damages may occur in steel structures due to inappropriate design, inadequacy of construction, improper uses and some other factors. Such damages could produce serious safety problems and shorten the service life of the structures. We proposed in this paper an effective and accurate method for analyzing the ultimate bearing capacity of steel framework based on the fiber hinge model and time-varying structure theory. A detailed study was conducted to investigate a cantilever column with I shaped cross section. The effects of cross section division and fiber hinge unit length on computation accuracy were investigated using SAP2000 finite element software. The proposed method was then applied to a real-life engineering problem, in which the steel structure of a mechanical building was gradually damaged under the action of uneven foundation settlement. The detailed information was obtained for the whole structural damage process as well as for the structural improvements after reinforcement. The results presented in this paper provided valuable information for strengthening of the damaged structures that are usually difficult to obtain in field detection, as well as the critical information for evaluating the structure conditions. In addition, the results in this paper could also provide useful information for safety assessment of new constructions.

Investigation of a new 3Y-stabilized zirconia with an improved optical property for applications as a dental ceramic

Yuhua Wang,Hui Huang,Limin Gao and,Fuqiang Zhang 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.4

To study the mechanical and optical properties of a new nanocrystalline yttria-stabilized zirconia (PX242A Tosoh Japan). The influence of heating rates on its properties and its potential use as a dental ceramic were also evaluated. Samples of PX242A were divided into 4 groups according to heating rates : group H1 (100oKh−1); group H2 (200oKh−1); group H4 (400oKh−1);group H6 (600oKh−1). Group H0 (3Y-SBE Tosoh Japan) was made according to the manufacturer’s recommendation as a control. The contrast ratio (CR), transparency parameter (TP) and total transmittance (TT) were measured by an Xrite spectrophotometer. The flexural strength and fracture toughness were also evaluated. The flexural strength and fracture toughness were found to be independent of the heating rate while the optical properties significantly increased when the heating rates were 600oKh−1, 200oKh−1 and 400oKh−1, but were not significantly different with 100oKh−1. A heating rate of 600oKh−1 is suggested as a time efficiency protocol, because it could provide desirable mechanical and optical properties,making zirconia suitable for clinical use.

Interleukin-10-Producing B Cells Help Suppress Ovariectomy-Mediated Osteoporosis

Yuhua Wang,Wei Zhang,임성민,Li Xu,진준오 대한면역학회 2020 Immune Network Vol.20 No.6

Osteoporosis is prevalent in elderly women and it may cause dental implant failure. In particular, estrogen deficiency in postmenopausal women leads to higher rates of osteoporosis prevalence. Immune cell-mediated effects involving the development of osteoporosis have been studied previously; however, the role of IL-10-producing regulatory B (B10) cells in osteoporosis is largely unclear. Here, we examined the role of B10 cells in osteoporosis. C57BL/6 mice were subjected to ovariectomy (OVX). Fifteen weeks after OVX surgery, the first molar of the right maxillary was extracted, and twenty-four weeks after OVX surgery, serous progression of osteoporosis was observed in the alveolar bone. Moreover, the proportion of CD19+CD5+CD1dhigh regulatory B cells, B10, and CD4+CD25+FoxP3+ regulatory T cells from the spleen of OVX mice decreased during the progression of osteoporosis, compared to controls. In contrast to regulatory cells, IL-17-producing Th (Th17) cell levels were increased in OVX mice. Adoptive transfer of B10 cells to OVX mice led to a decrease in Th17 cell abundance and inhibited the development of osteoporosis in the alveolar bone from OVX mice. Thus, our results suggest that B10 cells may help suppress osteoporosis development.

An Optimized Third Harmonic Injection Method for Three-Phase Cascaded H-Bridge Photovoltaic Inverter

Wang Mao,Xing Zhang,Tao Zhao,Yuhua Hu,Fusheng Wang,Zhiqiang Dai,Renxian Cao 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The uneven irradiation and temperature in photovoltaic (PV) modules of three-phase cascaded H-bridge (CHB) inverters will cause the unbalanced output power between solar panels, resulting in unbalanced grid current between three phases. In addition, since each phase can be regarded as a single-phase inverter, the second harmonic voltage pulsation of dc-side capacitors is a problem to be solved. To solve these two problems, a third harmonic injection optimization method on the basis of fundamental frequency zero sequence is proposed in this paper. The third harmonic with the same amplitude of fundamental modulated wave signal is injected into the modulated wave signal when the amplitude of synthesized wave signal is no greater than unity. Otherwise, the amplitude of third harmonic is decreased correspondingly to ensure the amplitude of synthesized wave signal being unity. With this method, the dc-side voltage pulsation is effectively restrained and the power balancing limit of CHB inverter is expanded. The feasibility and effectiveness of this method are verified by an experimental prototype of a three-phase seven-level CHB inverter.

A spherical oscillator gauge with carbon nanotube cathode

Yuhua XIAO,Detian LI,Yongjun WANG,Huzhong ZHANG,Shifa WANG,Yali LI,Danming LI,Yongjun CHENG 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Characteristics of lipoxygenase-based and lipoxygenase-deficient soy yogurt with modified okara

Xiujuan Wang,Yue Chen,Yuhua Wang,Weichang Dai,Chun Hong Piao,Hansong Yu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.13

Lipoxygenase-based and lipoxygenase-deficientokara were modified by Kluyveromyces marxianus fermentation,then adding modified okara back to the correspondingsoymilk to prepare soy yogurt. Thephysicochemical properties, texture, and volatile componentsof soy yogurt were characterized. The results showedthat okara modified by Kluyveromyces marxianus fermentationwas rich in soluable dietary fiber and was impartedbetter water-holding capacity, swelling capacity, and oilholdingcapacity. The soy yogurt with the modified okarawas greatly enhanced in its appearance, texture and wasrelatively stable during storage. Moreover, lipoxygenasebasedsoy yogurt had a unique soybean flavor whilelipoxygenase-deficient soy yogurt had a slight beany flavorand soybean flavor. This article guides a bio-modifiedmethod for okara and provides a theoretical basis for thefurther development and application of soy yogurt withhigh dietary fiber as well as lipoxygenase-deficient soyyogurt.

Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis

Yixin Wang,Long Jin,Jideng Ma,Li Chen,Yuhua Fu,Keren Long,Silu Hu,Yang Song,Dazhi Shang,Qianzi Tang,Xun Wang,Xuewei Li,Mingzhou Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. Methods: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. Results: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Conclusion: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.

Whey Protein Concentrate, Pullulan, and Trehalose as Thermal Protective Agents for Increasing Viability of Lactobacillus plantarum Starter by Spray Drying

Haiyue Sun,Xiaoman Hua,Minghao Zhang,Yu Wang,Yiying Chen,Jing Zhang,Chao Wang,Yuhua Wang 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.1

It is necessary to add protective agents for protecting the probiotic viability in the preparation process of probiotics starter. In this study, we used whey protein concentrate (WPC), pullulan, trehalose, and sodium glutamate as the protective agent and optimized the proportion of protective agent and spray-drying parameters to achieve the best protective effect on Lactobacillus plantarum. Moreover, the viable counts of L. plantarum in starter stored at different temperatures (–20℃, 4℃, and 25℃) for 360 days were determined. According to response surface method (RSM), the optimal proportion of protective agent was 24.6 g/L WPC, 18.8 g/L pullulan, 16.7 g/L trehalose and 39.3 g/L sodium glutamate. The optimum spray-drying parameters were the ratio of bacteria to protective agents 3:1 (v: v), the feed flow rate 240 mL/h, and the inlet air temperature 115℃ through orthogonal test. Based on the above results, the viable counts of L. plantarum was 12.22±0.27 Log CFU/g and the survival rate arrived at 85.12%. The viable counts of L. plantarum stored at –20℃ was more than 1010 CFU/g after 200 days.

Influence of some key factors on material damping of steel beams

Wang, Yuanfeng,Pan, Yuhua,Wen, Jie,Su, Li,Mei, Shengqi Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.49 No.3

Material damping affects the dynamic behaviors of engineering structures considerably, but up to till now little research is maintained on influence factors of material damping. Based on the damping-stress function of steel, the material damping of steel beams is obtained by calculating the stress distribution of the beams with an analytical method. Some key influence factors of the material damping, such as boundary condition, amplitude and frequency of excitation, load position as well as the cross-sectional dimension of a steel beam are analyzed respectively. The calculated results show that even in elastic scope, material damping does not remain constant but varies with these influence factors. Although boundary condition affects material damping to some extent, such influence can be neglected when the maximum stress amplitude of the beam is less than the fatigue limit of steel. Exciting frequency, load position and cross-section dimension have great effects on the material damping of the beam which maintain the similar changing trend under different boundary conditions respectively.