혼합형 자가면역성 용혈성 빈혈을 동반한 전신성 홍반성 루푸스 1예

이승철,김선임,이승훈,이현경,조남국,한성훈,유영진,조종래 인제대학교 2000 仁濟醫學 Vol.21 No.2

A 17-year-women developed mixed warm and cold antibody type autoimmune hemolytic anemia associated with systemic lupus erythematosus. The patients was admitted to our hospital for syncope. Jaundice was observed. Urinalysis revealed protein and hemoglobin. Her hemoglobin was 4.6g/dl and reticulocytes were 10%. Total bilirubin was elevated, while haptoglobin and complement were abnormally reduced. ANA and mitochondrial Ab were detected. The direct and indirect Coombs test were positive. The direct antiglobulin test was positive ; IgG and C3d were detected on the red cell surface. The cold agglutinin(CA) titer was 128. The patients was initially treated with high dose steroid therapy. However the patients did not responded to high dose steroid therapy. We treated the patients with plasmapheresis and synchronized cyclophosphamide therapy. Clinical remission of hematologic complications was achieved in this patients. We report a case of mixed type autoimmune hemolytic anemia with SLE that responded plasma pheresis and synchronized cyclophosphamide therapy.

Quantitative comparison of mRNA expression of glucosyltransferase(GTF) between xylitol-resistant(X^(R)) and xylitol-sensitive(X^(s)) mutans streptococci

Kim, Chong-Chul,Lee, Mi-Na,Kim, Young-Jae,Lee, Sung-Hoon 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Since the long-term exposure of mutans streptococci to xylitol is known to select for xylitol-resistant(X^(R)) natural mutants, the occurrence and survival of such X^(R) strains were performed in batch culture methods. The aim of the study was to compare the differentiation and quantification of mRNA expression of the gtf genes of X^(R) and X^(S) mutans streptococci. Using a real-time reverse-transcription polymerase chain reaction, the expression of each gtf was determined. In X^(R) strains, the relative levels of transcription of gtfB and gtfC were decreased while that of gtfD was increased, suggesting the presence of independent promoters. It also suggested that mutation related to production of glucosyltransferase occurred under the exposure of xylitol could explain the caries-preventive mechanisms of xylitol. 자일리톨에 장기간 노출된 mutans streptococci는 자일리톨에 내성이 발현되어 자일리톨 내성균주가 생성된다고 알려져 있다. 본 연구의 목적은 mutans streptococci에서 자일리톨 내성균주와 감성균주의 gtf 유전자 발현량을 각각의 유전자별로 정량적으로 분석하고 비교하는 것이다. 실시간 역전사 중합효소연쇄반응법을 이용하여 각각의 gtf 발현을 조사한 결과 gtfD는 증가한 반면. gtfB와 gtfC는 감소하였는데 이는 각 유전자의 독립된 조절기전이 존재함을 보여주는 것이다. 또한 자일리톨에 노출된 mutans streptococci에서의 glucosyltransferase와 연관된 유전자변형이 자일리톨의 치아우식증 예방효과의 작용기전 중 하나임을 알 수 있었다.

Wiskott-Aldrich 증후군 환아의 증례보고

이연주,현홍근,장철호,김영재,김정욱,장기택,김종철,한세현,이상훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

인체지방기질세포를 통한 척수 손상에 대한 신경학적 회복

이태훈,신철호 남서울대학교 2010 남서울대학교 논문집 Vol.16 No.1-1

중추신경계 질환의 회복은 손실된 세포재생, 손상된 수초대체, 기능적 재연결을 담당하는 척추중추신경계의 제한적 기능으로 인해 저해된다. 중추신경계 질환의 치료방법으로 줄기 세포 이식은 기능적 결함을 감소시킬 수 있는 중요연구 과제로 부상하고 있다. 본 연구에서는 척수 손상된 백서에 인체지방기질세포의 이식을 통한 효과를 검증하는데 목적을 둔다. 척수 손상은 흉수신경 9번째와 10번째에서 혈관용 클립을 통해 유발되었으며 척수 손상후 7일후에 지방기질세포의 이식을 시행하였다. 척수 손상된 동물 모델에 CM-Dil로 염색된 지방기질세포를 이식하게 되면 척수 손상부위에 지방기질세포들이 채워질 것으로 추측된다. 또한 지방 기질세포로 이식한 동물에서 행동측정지침인 BBB 측정값이 높으며 척수 손상 정도가 조직학적으로 감소될 경우 이식한 지방기질세포들이 신경세포나 성상세포로는 분화되지는 않을 경우로 간주될 것이다. 예상한 결과들은 지방으로부터 분리된 기질세포가 신경자극인자들을 분비하거나 손상부위의 조직과 직접적으로 접촉함으로서 기능적, 행동적 향상에 기여한다고 볼 수 있다. 본 연구 결과는 척수 손상 치료에 지방기질세포의 임상적 응용 가능성을 제안한다. 핵심용어 : 중추신경계, 척수 손상, 인체지방기질세포

영구치의 맹출 장애에 관한 조사 연구

이종범,장철호,김종철,한세현,이상훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

맹출 중인 치아는 오랜 기간 동안 이동하는 과정에서 주위의 여러 요인들, 즉 유치의 조기 상실, 과잉치, 국소적 병소, 혀, 입술, 저작근, 외상 등에 의해서 다양한 영향을 받을 수 있다. 이러한 여러 요인들이 작용할 경우 치아의 정상적인 맹출 과정이 방해를 받게 되어 치아의 맹출 장애를 초래할 수 있다. 본 연구에서는 서울대학교 치과병원 소아치과에 2004년 1월 2일부터 2005년 12월 31일까지 신환으로 내원한 환자들, 총 8,010명의 주소를 분석하였고, 맹출 장애를 보이는 환자는 전체의 8.79%를 보였다. 맹출 장애의 유형은 맹출 지연이나 조기 맹출, 이소 맹출로 분류할 수 있었고, 맹출 지연이 가장 많이 나타난 치아는 상악 중절치이며, 조기 맹출이 호발한 치아는 하악 유중절치이고, 이소 맹출이 가장 많이 나타난 치아는 상악 제1대구치였다. Eruption disturbance is an abnormal state of eruption that occurs over a broad chronologic age range. There are many factors that influence eruption such as premature loss of primary teeth, supernumerary tooth, local disease, tongue, lip, mastication muscle and trauma. The purpose of this study was to determine the prevalence, patterns and tooth position that have eruption disturbance in children in Seoul, Korea. The subjects were 8,010 new patients who visited the department of Pediatric dentistry of Seoul National University Dental Hospital in 2004-2005. Clinical and radiographic records of the patients were reviewed by the primary investigator who is a pediatric dentist. Notable eruption disturbances included delayed eruption, early eruption and ectopic eruption and the prevalence of eruption disturbance was 8.79%.

캔음료중 epichlorohydrin 의분석법에 관한 연구

이광호,곽인신,최재천,전대훈,김형일,강경모,최병희,김귀정,이철원 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

캔식품중에 잔류하는 epichlorohydrin을 분석하기 위한 pu링e & trap-Cc/uf통D 분석법을 확립하여 캔의 lacauer 코팅제에서 식품으로 이행되어 잔류하는 epichtorohydfn을 측정하고, 식품유사용매에 의한 용출시험을 통해 일일추정섭취량을 산출하였다. epichlorohydrin의 검량선은 y=0.0006x+0.OO6?(R큰0.9983)의 직선성·라 0.05#ga의 검출한계를 나타내었다. 각종 음료 및 식품유사용매에 일정량의 epichlorohydrin을 첨가한 후 분석하였을 때 회수을은 72~91%였으며, 실제 시판되는 f5건의 캔음료 중 잔류하는 epichlorohydrin을 측정한 결과 모든 캔음료에서 검출한계(0.1#94) 이하였다. 제관업체에서 구입한 modifed epoxy, epoxy phenolic, vinyl organosol, epoxy phenol ester 등의 BADGE(bisphenol A diglycidyt ether)형 에폭시수지로 lacquer 코팅된 식품용 캔에 식품윤사용매인 10% 또는 95% 에탄올을 층L진한 후 90~121'c의 드라이오븐에서 30분~2시간동안 용출시켰을 패 캔의 lacquer 코팅재에서 식품유사용매로 이행되는 epichBorollydirn은 코팅재질, 그리고 가열온도 및 시간에 문관하게 모든 캔에서 검출한계(0.4#ga)이하 였다. 위의 용출결과를 FD.f_의 "Recommendation for Chemistry Data for Indirect Food Additive Petition" 윽 일일추정섭취량 산출법에 적용하여 얻은 epichtorohydrin의 일딜추정 섭 취 량은 0.2r조persorday이하였다. A sensitive ana1?~7tical method based on gas cl)romatogFapy~mass spectrometry with a selected ion monitoring (GE/hfS-Slhf) and the purge-and-trap concentrator ))·asdeveloped for determining ot'epicillorohydrin in canr;ed be)#erages coated with eporl· resin. Thecalibration rurve in the ranre of 0.j ~50nr had'correlation coefficient of 0.9983 and detection limit of 0.Osrga. Recoveries of epichlorohydrin spiked to beverages were in the range of 72 ~91% with detection limits of 0.1 #ga. fn survey of epichlorohydff in twenty commercialcanned beverage samples, epichlorohydrin was not found in all the samples with the detectionlimit of 0.1 r9a. In migration test of various cans coated with BADGE-based coating cans;modifed epoxy, epoxy phenolic, vinyl organosol, epox?· phenol ester were exposed to the foodsimulants of 10% ethanol and 95% ethanol. flfo delectable epichlorohydrin migrated to foodsiHulants was found in aTl the cars with detection Jimit of 0.4 #9/L. Using the miEration data,along uTith the applicstion of food and beverage cans complied with FDA's "Recommendationfor Chemlstry Data f,3r Indirect Food Additive Petition" , the potential dietary exposure toepichlorohydrin was estlmated to be under 0.2rf/person/day.

Vancomycin B 의 불순물 연구

이철훈,경한수,임융호 建國大學校附設 農業資源開發硏究所 1998 農資源開發論集 Vol.20 No.-

Vancomycin B obtained by fermentation includes its analogs. Even though the amounts of the analogs are very little because of careful purification steps, those can give rise to toxicities. Therefore, impurities contained in vancomycin B produced by fermentation should be identified. A pyridine buffer was applied for separation of impurities, and good NMR spectra were collected except more downfield shifts than 7 ppm. Four impurities included in vancomycin B were identified.

자동차 관련직종 근로자에서 상지 근골격계 증상 호소율과 관련요인

이세훈,윤철수 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : The purpose of this study was to determine the prevalence and related factors of upper limb musculoskeletal symptoms among workers in automobile related jobs. Methods : Two-hundred and twenty-one workers volunteered in this study consisting of 112 seat installers and 109 auto mechanics. A questionnaire was administered to the workers consisting of questions on demographics, work type and duration, sleep hours, health habits, and standardized descriptions of NIOSH on musculoskeletal symptoms. Results : Complaint rates of neck and upper extremity musculoskeletal symptoms by anatomical site were as follows: shoulder, 52.0%; neck, 47.1%; wrist and hand 39.4%; elbow, 26.2% and; arm, 24.4%. Significantly higher prevalence of shoulder musculoskeletal symptom was found in middle school than high school graduate, married than unmarried, less than 6 hours sleep than over 6 hours sleep and, seat installer than auto mechanic group. A significantly higher prevalence of arm, elbow, hand and wrist musculoskeletal symptoms was disclosed in the less than 6 hours sleep than over 6 hours sleep group and the seat installer than the auto mechanic group. Multiple logistic regression analysis identified education, sleep hours and, job type as the main affecting factors for shoulder musculoskeletal symptoms. Sleep hours and job type were the main affecting factors for wrist and hand musculoskeletal symptoms. Sleep hours were the main affecting factors for arm and elbow musculoskeletal symptoms. Conclusions : The prevalence of neck and upper limb musculoskeletal symptoms was high in workers who did routine tasks at confined places like seat install-line work. It is recommended that workers performing simple and routine tasks be provided adequate sleep time since lack of sleep was the main affecting factor for most upper limb musculoskeletal symptoms in automobile factory related jobs workers.

Cyclin-dependent kinase(CDK)와 세포주기(cell cycle)조절을 통한 새로운 항암제의 개발 현황

이철훈,조율희 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.1

The cell-division cycle is a tightly controlled process that is regulated by the cyclin/CDK family of protein kinase complexes. Stringent control of this process is essential to ensure that DNA synthesis and subsequent mitotic division are accurately and coordinately executed. There in now strong evidence that CDKs, their regulators, and substrates are the targets of genetic alteration in many human cancers. As a result of this, the CDKs have been targeted for drug discovery and a number of small molecule inhibitors of CDKs have been identified. Our attempt here is to illustrate the potential for development of therapeutics to treat human cancers by interfering with cell-cycle progression. Because of the central role that they play in advancing the division cycle, CDKs have been targeted for drug discovery and a number of small molecule compounds have now been identified as CDK inhibitors. These strategies and other targets of intervention within the cell cycle are discussed in our review.

자연파열된 신혈관근지방종 2례

이길호,선판일,성영훈,최영석,조철희,김철성,장대수 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Renal angiomyolipoma is an uncommon benign tumor of the kidney that consists of smooth muscle, blood vessels and mature adipose tissues. The most common benign renal tumor associated with spontaneous non-traumatic perirenal hemorrhage is angiomyolipoma, because of high vascularity. The ultrasonographic and computed tomographic images are sufficiently characteristic to permit accurate preoperative diagnosis. We report 2 cases of renal angiomyolipoma with spontaneous perirenal hemorrhage which were managed with nephrectomy.