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사전령,권동주,김진규,황병호,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-
일본잎갈나무 낙엽 (8.5kg)을 채취하여 9.5% EtOH 용액으로 추출하고 농축된 추출용액은 분획깔때기로 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성과 수용성 분획에 대하여 칼럼크로마트크래피를 실시하였고, 충진물질로는 Sephadex LH-20을, 용리용매로는 메탄올 수용액 및 에탈올-핵산 혼합용액을 사용하였다. 단리된 화합물들은 TLC로 확인한 후 NMR 스펙트럼을 사용하여 정확한 구조규명을 하였고 FAB-MS와 EI-MS 스펙트럼으로 분자량을 측정하였다. 일본잎갈나무 낙엽으로부터 5개의 화합물을 단리하였으며, 각 분획물과 단리된 화합물들은 DPPH 라디칼 소거법을 이용하여 항산화 시험을 실시하였다. Fallen neeldes (8.5kg) of Larix kaempferi were separately collected. extracted with 95% EtOH. EtOH extract was evaporated under reduced pressure, concentrated then successively fractionated with a series of hexane. methylene chloride, ethylaectate and water on a separatory funnel. Then, each fraction was freeze dried. A portion of ethylacetate and water soluble powder were packed on a column chromatography (Sephadex LH-20) eluting with aqueous MeOH and EtOH-hexane mixture. Spectrometric analyses such as NMR and FAB-MS including TLC were performed to characterize the structures of isolated compounds.
사전령,김진규,권동주,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-
강원대학교 구내림에서 오동나무 잎을 채취하여 아세톤-물(7:3, v/v)로 추출하고 n-hexane, methylene chloride, ethylacetate 및 H_(2)O 등 네 개의 분획으로 분리한 후 에틸아세테트용성 분획물을 Sephadex LH-20으로 칼럼 크로마토그래피를 수행하였으며 용리용매는 메탄올 수용액과 에탄올-핵산 혼합액을 사용하였다. 그 결과 flavonoid류 화합물인( +)-catechin, ( - )-epicatechin, apigenin, luteolin, taxifolin과 phenolic acid류 화합물인 p-hydroxybenzoic acid, vanillic acid 및 p-coumaric acid를 단리 하였다. 단리된 화합물은 NMR 및 MS 스펙트럼을 이용하여 구조를 결정하였다. The leaves of Paulownia Coreana Uyeki were collected, extracted with acetone-H_(2)O(7:3, v/v), fractionated with n-hexane, methylene chloride and ethylacetate, and freeze dried to give some dark brown powder. The ethylacetate soluble mixture was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. Spectrometric analysis such as NMR and MS From the ethylacetate fraction, five flavonides and three phenolic acids were isolated and determined.
Chuan-Te Tsai,Yung-Ming Chang,Shu-Luan Lin,Yueh-Sheng Chen,Yu-Lan Yeh,Viswanadha Vijaya Padma,Chin-Chuan Tsai,Ray-Jade Chen,Tsung-Jung Ho,Chih-Yang Huang 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.3
Angiotensin II (Ang II) is a very important cardiovascular disease inducer and may cause cardiac pathological hypertrophy and remodeling. We evaluated a Chinese traditional medicine, alpinate oxyphyllae fructus (AOF), for therapeutic efficacy for treating Ang II-induced cardiac hypertrophy. AOF has been used to treat patients with various symptoms accompanying hypertension and cerebrovascular disorders in Korea. We investigated its protective effect against Ang II-induced cytoskeletal change and hypertrophy in H9c2 cells. The results showed that treating cells with Ang II resulted in pathological hypertrophy, such as increased expression of transcription factors NFAT-3/p-NFAT-3, hypertrophic response genes (atrial natriuretic peptide [ANP] and b-type natriuretic peptide [BNP]), and Gαq down-stream effectors (PLCβ3 and calcineurin). Pretreatment with AOF (60–100 μg/mL) led to significantly reduced hypertrophy. We also found that AOF pretreatment significantly suppressed the cardiac remodeling proteins, metalloproteinase (MMP9 and MMP2), and plasminogen activator, induced by Ang II challenge. In conclusion, we provide evidence that AOF protects against Ang II-induced pathological hypertrophy by specifically inhibiting the insulin-like growth factor (IGF) II/IIR-related signaling pathway in H9c2 cells. AOF might be a candidate for cardiac hypertrophy and ventricular remodeling prevention in chronic cardiovascular diseases.
Roles of mTOR and p-mTOR in Gastrointestinal Stromal Tumors
Li, Jun-Chuan,Zhu, Hong-Yu,Chen, Ting-Xuan,Zou, Lan-Ying,Wang, Xiao-Yan,Zhao, Hui-Chuan,Xu, Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Objective: This study aimed to examine the relationship between expression of mammal target of rapamycin (mTOR) and phosphorylation of mTOR (p-mTOR) protein in the PI3K/Akt/mTOR signaling pathways in gastrointestinal stromal tumors and relatiuonships with clinical factors. Methods: Immunohistochemistry was used to detect the expression of the associated proteins mTOR, p-mTOR, and phosphorylation of the tumor suppressor genes PTEN, P27, VEGF, and EGFR in 40 cases of gastrointestinal stromal tumors, with division into a very low and low risk group as well as a moderate and high risk group. Results: The positive rate of mTOR and p-mTOR was significantly increased in the moderate and high risk group compared with the very low and low risk group. The difference was statistically significant (P<0.05). When grouped according to size, the positive mTOR expression rate exhibited a statistical difference (P<0.05), which was significantly increased in the group of tumors larger than 5 cm. The difference in the positive mTOR and p-mTOR expression rate exhibit no statistical significance among the PTEN, P27, VEGF, and EGFR expression subgroups (P>0.05). Conclusion: The different expressions of mTOR and p-mTOR in the signal transduction pathway of gastrointestinal stromal tumor in the different degree-of-risk groups suggested that the mTOR and p-mTOR of the signal transduction pathway serve an important function in the occurrence and development of gastrointestinal stromal tumors.
Agent Bidding Strategy of Multiple Round English Auction based on Genetic Network Programming
Chuan Yue,Shingo Mabu,Yan Chen,Yu Wang,Kotaro Hirasawa 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
The auction mechanism is widely used in web-based sites and originally designed for human beings, but it might not be the most efficientonein the future, while, there is ademand of evolutionary computation auction agents adaptable to the dynamic auction environments. In this paper, we have applied Genetic Network Programming(GNP) to auction agents to determine a bid at each time step and developed multiple round English Auction mechanisms based on multi-agent systems. In the simulations, we provide comparisons of the proposed method with existing ones. As a result, it has been found that the proposed method could help agents to evolve their strategies generation by generation to get more goods with less money. Also, GNP has a good performance of helping the agent to find out the suitable strategy under the current situation.
Quadrotor waypoint navigation and trajectory tracking using backstepping technique
Chuan Lian Zhang,Kill To Chong 대한전기학회 2015 정보 및 제어 심포지엄 논문집 Vol.2015 No.4
Researchers have given much attention on quadrotor due to its practical military and civilian application. Quadrotor is a system which has complex dynamics, nonlinearities and high degree of coupling between control inputs and state variables. To confront these problems, this paper developed a backstepping controller for quadrotor waypoint navigation and trajectory tracking control, and the controller can set quadrotor track desired positions(x, y, z) and yaw angle while stabilize pitch and roll angles. Besides that, a simple path planning algorithm was presented to get a time-parameterized polynomial trajectory between two points. Simulations have been done on waypoint navigation and trajectory tracking and simulation results showed that backstepping controller got good performance.
Quadrotor waypoint navigation and trajectory tracking using backstepping technique
Chuan Lian Zhang,Kill To Chong 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.4
Researchers have given much attention on quadrotor due to its practical military and civilian application. Quadrotor is a system which has complex dynamics, nonlinearities and high degree of coupling between control inputs and state variables. To confront these problems, this paper developed a backstepping controller for quadrotor waypoint navigation and trajectory tracking control, and the controller can set quadrotor track desired positions(x, y, z) and yaw angle while stabilize pitch and roll angles. Besides that, a simple path planning algorithm was presented to get a time-parameterized polynomial trajectory between two points. Simulations have been done on waypoint navigation and trajectory tracking and simulation results showed that backstepping controller got good performance.