허혈 및 재관류후 흰쥐 앞정강근과 가자미근에서 나타나는 미세구조와 SOD 활성의 변화

백두진,안동춘,황규성,김혜주,박철홍,정호삼,조근열 대한체질인류학회 1999 해부·생물인류학 (Anat Biol Anthropol) Vol.12 No.2

에너지 획득방법, 당원의 분포, 모세혈관의 분포 및 미세구조의 차이가 나는 백색근육섬유와 적색근육섬유에서 허혈시간에 따라 나타나는 미세구조의 변화와 SOD의 활성변동을 비교 관찰하여 다음과 같은 결과를 얻었다. 1. 앞정강근에서는 2시간 허혈시 사립체에서 바탕질의 전자밀도가 감소하고 사립체능선이 팽대하였고, 재관류 24시간 경과시에는 근육원섬유사이의 거리가 멀어지고 근육세포질세망의 수조와 종말수조가 팽대하였으며 재관류 72시간 경과시에는 정상대조군과 유사한 소견이 나타났다. 2. 가자미근에서 2시간 허혈시 사립체에서는 사렵체바탕질의 전자밀도가 감소하였으며, 재관류 24 시간 경과시는 근육세포질세망의 수조가 팽대하였고, 재관류 72시간 경과시에는 정상대조군과 유사하였다. 3. 4시간 및 6시간 허혈시 미세구조의 변화는 허혈시간과 재관류 시간경과에 따라 심해져 앞정강근에서는 사립체에서 바탕질의 전자밀도가 감소하고 사렵체능선은 불분명하고 소용돌이소체가 나타났으며 근육원섬유 용해와 곤육원섬유괴사가 관찰되었다. 가자미근에서는 근육원섬유에서 근육미세섬유의 배열과 끝가로막이 불규칙해지고 근육세포질세망의 수조가 팽대하였으며 근육원섬유용해가 나타났다. 4. 앞정강근에서 Cu, Zn-및 Mn-SOD 활성은 미약한 흑은 약한 양성반응을 나타내었고, 가자미근에서는 Cu, Zn-SOD는 미약한 양성반응을, Mn-SOD 는 미약한 혹은 약한 양성반응을 나타내었다. 허혈후 Cu, Zn- 및 Mn-SOD 의 활성은 허혈시간이 길수록 더욱 증가하였다. 5. Cu, Zn- 및 Mn-SOD 활성은 2시간 허혈 및 재관류 24 시간 경과시 약간 증가하였으며 72시간 경과시에는 앞정강근과 가자미근에서 정상대조군과 유사하게 나타났다. 6. 4시간 및 6시간 허혈 및 재관류후 두 근육에서 Cu, Zn- 및 Mn-SOD활성은 증가하였다. 두 근육에서 Mn-SOD의 활성변동은 유사하였으나 Cu, Zn- Mn-SOD의 활성증가는 앞정강근보다 가자미근에서 크게 나타났다. 이상의 결과를 종합하면 허혈 및 재관류 손상은 허혈시간과 재관류시간 경과에 따라 심하게 나타났고 가자미근보다 앞정강근에서 심하게 나타났으며, 손상의 차이는 두 근육에서 나타나는 SOD활성변동과 깊은 연관이 있는 것으로 생각된다. Skeletal muscles are known to have tolerance to ischemia, but a prolonged ischemia can cause damage to muscular tissues. The ischemia-reperfusion injury results from the oxygen free radicals released by leucocytes and formed by the reaction of hypoxanthine and xanthine oxidase. Superoxide dismutase (SOD), one of major antioxidant enzymes occurring in the various tissues of the body metabolizes or scarvanges the oxygen free radicals. Although many studies reported difference in tolerance to ischemia and reperfusion between white and red muscles, some other investigators failed in finding such difference. The present study was performed to examine effects of graded periods of ischemia and reperfusion on the cellular ultrastructure and activity of SOD in white and red muscles. The Sprague-Dawley rats (200~250 g) were used as experimental animals. Under pentobarbita] (50 mg/kg IP) anesthesia, incision was made on lower abdomen and left common iliac artery was occluded by means of a vascular clamp for 2, 4 and 6 hour (hrs). Thereafter, the superficial portion of mid-belly of anterior tibial muscle and soleus muscles were excised at 0, 24 and 72 hrs after onset of reperfusion. The specimens were sectioned into slices, 2 mm in length, 1 mm in width and thickness. Some specimens were prepared for electron microscopic observation and others for determination of SOD activity by using antihuman CU, Zn- and Mn-SOD antibodies. The results obtained were as follows. 1. In anterior tibial muscle, areas with loose electron-density and dilated cristae were observed in the mitochondria immediately after 2 hrs of ischemia, while widened intermyofibrillar spaces and dilated cisternae of sarcoplasmic reticulum were seen after 2 hrs and 24 hrs reperfusion. When subjected to 2 hrs ischemia and 72 hrs reperfusion, no significant change was found in the cellular ultrastructure. 2. In soleus muscle, electron density was loose in the matrix of mitochondria immediately after 2 hrs of ischemia, while cisternae of sarcoplasmic reticulum were dilalated after 2 hrs of ischemia and 24 hrs reperfusion. Following 2 hrs of ischemia and 72 hrs repeifusion, the electron microscopic findings were similar to those of normal rats. 3. The changes in cellular ultrastructure were more prominent in both the 4 hrs and 6 hrs ischemia groups, in which degree of ultrastructural changes were proportional to duration of reperfusion. 4. In anterior tibial muscle, trace or weak immunoreactivities of Cu, Zn- and Mn-SOD were seen, whereas trace immunoreactivity of Cu, Zn-SOD and trace or weak immunoreactivity of Mn-SOD were observed in soleus muscle. 5. The immunoreactivities of CU, Zn- and Mn-SOD were not altered in 2 hrs ischemic and 72 hrs reperfused group, while they were increased slightly in 2 hrs ischemic and 24 hrs reperfused group. 6. In both muscles, the activity of SOD increased following 4 hrs or 6 hrs ischemia and 24 hrs or 72 hrs reperfusion. The changes in immunoreactivity of Mn-SOD were not different between two muscles, whereas immunoreactivity of Cu, Zn-SOD were higher in anterior tibial muscle. Consequently, it is suggested that significant ischemia reperfusion injuries are produced after 4 ~ 6 hrs ishemia followed by 24 hrs or 72 hrs reperfusion, that anterior tibial muscle is more susceptible to ischemic reperfusion injury and that the ischemic-reperfusion injury is closely related with activity of SOD.

유한요소법을 이용한 기둥형 로드셀의 비선형성 평가

배준우,황재근,박경흠,양희준,이춘열 嶺南大學校 工業技術硏究所 2004 工業技術硏究所論文集 Vol.32 No.1

Recently, force measurement systems are commonly used in many industrial fields and the precision of the measurement system is getting more important as the industry needs more precise tools and instruments to make high quality products. However, a high precision force measurement system is hard to make unless we know precisely the causes, quality and quantity of measurement errors in advance. In this work, many possible mechanical causes of measurement errors are reviewed including ratio of length to diameter of sensing part, radius of contact area, radius of bearing part, ratio of material properties and change of boundary conditions. Also, the measurement errors are analyzed by nonlinear finite element method and the nonlinear behavior of the errors are investigated. The results can be used to design force measurement systems and expected to be very useful especially for compact type load cells.

암모니아 및 아질산성 질소 산화세균의 분리 및 특성

이용석,유주순,정수열,박춘수,최용락 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

본 연구는 폐수 중의 질소 제거를 위한 생물학적 처리용 미생물 개발을 위한 목적으로 질소의 산화 능력이 뛰어난 균주를 분리하였다. 분리된 세균 중에서 질소 산화능과 생육 속도가 뛰어난 CH-N 균주를 선별하였으며, 생리, 생화학적인 특성 조사에 의해 Bacillus sp로 추정되어 Bacillus sp. CH-N이라 명명하였다. 분리 균주는 0.5% glucose가 포함된 초기 pH가 7.0인 암모니아 및 아질산성 질소 함유 배지에서 30시간 배양 후 가가 85%와 90%의 암모니아성과 아질산성 질소가 단시간에 질소의 감소율을 나타내었다. 폐수 및 생활하수에 분리 균주를 이용한 결과, 수질 속의 암모니아성 질소가 단시간에 크게 감소시키는 효과를 확인하였다. 균주를 고정시킨 담체의 질소산화 효과를 시험하고자 Bacillus sp. CH-N을 고정시킨 세라믹 담체를 이용한 결과, 배양 2일 후에는 암모니아성 질소가 전부 제거되었다. In order to improve the system for biological nitrogen oxidizing process in sewage and wastewater, a bacterium having high abilities to oxidize of nitrogen was isolated from wastewater and polluted soils. The strain was identified to Bacillus sp. CH-N, based on the physiological and biochemical properties. Characteristics and oxidizing ability of both ammonia and nitrite were examined for the strain, Bacillus sp. CH-N. The strain showed the oxidizing rate about 80% to 90% on the sewage and wastewater after 48 h culture. The nitrogen oxidizing rate was increased in proportion to the initial concentration of glucose. The microorganism, Bacillus sp. CH-N cell immobilized on ceramic carrier were evaluated for the oxidation of ammonia in culture media.

Mallory-weiss "Syndrome as the First Bleeding Manifestation in a 34-year-old Male Patient with Severe Hemophilia B.

Ha Yeol Park · Choon Hae Chung · Chi Young Park · Sang Gon Park · Hyung Jin Park 조선대학교 의학연구원 2011 The Medical Journal of Chosun University Vol.36 No.4

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A rare case of duodenal small cell carcinoma

( Ha Yeol Park ),( Choon Hae Chung ),( Chi Young Park ),( Sang Gon Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

The length of small intestine is about 75% of total gastro-intestinal tract, but the incidence of malignacy is account for 2% of it. Whereas most of small intestinal malignancy is duodenal cancer, and the most common histologic type is adenocarcinoma. Duodenal small cell carcinoma is extremely rare. We had a case of a 66-year-old female who visited the hospital to evaluation for RUQ discomfort. Endoscopy was done and large ulcerative lesion is founded at duodenum. The histologic finding was small cell carcinoma. We performed Abdomen-pelvis CT and bladder wall thickening was seen. For confirm the lesion, cystoscopic biopsy of bladder wall was done and pathologic finding was small cell carcinoma, too. In positron emission tomography-computed tomography (PET-CT), we can find multiple metastasis to duodenum, bladder, lung and brain. Finally we diagnosed duodenal small cell carcinoma with multiple metastasis. After 4-cycles of chemotherpy, Irinotecan and Cisplatin, the mass size was markly decreased. However she was expired due to unknown origin infection. We present a extremly rare case of duodenal small cell caricnoma with bladder wall, lung and brain.

Chemotherapy response related massive bleeding and management

( Ha Yeol Park ),( Choon Hae Chung ),( Chi Young Park ),( Sang Gon Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

The goals of palliative chemotherapy are to improve overall survival and the quality of life through relief of symptoms.The other hand, chemotherapy has many side effects such as myelosupression, neuropathy, gastro-intestinal symptom and infertility. In many known side effects of chemotherapy, bleeding occurs in approximately 6-10% of patients with advanced cancer. Most of bleeding is local vessel damage and systemic process such as disseminatated intravascular coagulopathy, abnormalities in platelet function or number after chemotherapy. There is no article about chemotherapy response related bleeding. However, bleeding may occur in chemo-sensitive tumor like lymphoma and in pre radiotherapy patient such as head and neck cancer, rectal cancer after chemotherapy. There are three cases of pure chemotherapy response related bleeding without other bleeding condition. One is pre-radiotherapy given maxillary sinus cancer, another is chemo sensitive gastric cancer and the last case is pre-radiotherapy given rectal cancer. These all cases had a massive bleeding after chemotherapy without other bleeding condition and successful management variable method such as embolization, endoscopy, and thrombotic agent.

A rare case of duodenal small cell carcinoma

( Ha-yeol Park ),( Choon-hae Chung ),( Chi-young Park ),( Sang-gon Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

Chemotherapy response related massive bleeding and management

( Ha Yeol Park ),( Choon Hae Chung ),( Chi Young Park ),( Sang Gon Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

Inactivation of JAK2/STAT3 Signaling Axis and Downregulation of M1 mAChR Cause Cognitive Impairment in klotho Mutant Mice, a Genetic Model of Aging

Park, Seok-Joo,Shin, Eun-Joo,Min, Sun Seek,An, Jihua,Li, Zhengyi,Hee Chung, Yoon,Hoon Jeong, Ji,Bach, Jae-Hyung,Nah, Seung-Yeol,Kim, Won-Ki,Jang, Choon-Gon,Kim, Yong-Sun,Nabeshima, Yo-ichi,Nabeshima, American College of Neuropsychopharmacology 2013 Neuropsychopharmacology Vol.38 No.8

We previously reported cognitive dysfunction in klotho mutant mice. In the present study, we further examined novel mechanisms involved in cognitive impairment in these mice. Significantly decreased janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3) phosphorylation were observed in the hippocampus of klotho mutant mice. A selective decrease in protein expression and binding density of the M1 muscarinic cholinergic receptor (M1 mAChR) was observed in these mice. Cholinergic parameters (ie, acetylcholine (ACh), choline acetyltransferase (ChAT), and acetylcholinesterase (AChE)) and NMDAR-dependent long-term potentiation (LTP) were significantly impaired in klotho mutant mice. McN-A-343 (McN), an M1 mAChR agonist, significantly attenuated these impairments. AG490 (AG), a JAK2 inhibitor, counteracted the attenuating effects of McN, although AG did not significantly alter the McN-induced effect on AChE. Furthermore, AG significantly inhibited the attenuating effects of McN on decreased NMDAR-dependent LTP, protein kinase C βII, p-ERK, p-CREB, BDNF, and p-JAK2/p-STAT3-expression in klotho mutant mice. In addition, k252a, a BDNF receptor tyrosine kinase B (TrkB) inhibitor, significantly counteracted McN effects on decreased ChAT, ACh, and M1 mAChR and p-JAK2/p-STAT3 expression. McN-induced effects on cognitive impairment in klotho mutant mice were consistently counteracted by either AG or k252a. Our results suggest that inactivation of the JAK2/STAT3 signaling axis and M1 mAChR downregulation play a critical role in cognitive impairment observed in klotho mutant mice.

간경변 환자에서 위배출시간에 영향을 주는 요인

박수헌,김진일,정인식,김재광,정규원,한준열,선희식,손형선,방춘상,나종순 대한소화기학회 1998 대한소화기학회지 Vol.32 No.1

Background/Airns: Although patients with cirrhosis often complain the gastrointestinal symptoms such as indigestion, little attention has been paid to the possible effects of portal hypertension on gut function. Frequently, endoscopic feature of patients with cirrhosis included congestive gastropathy. However, it has not been shown that whether gastric emptying time is delayed in cirrhotic patients with congestive gastropathy. The aims of this study were to evaluate the gastric emptying in cirrhotic patients with congestive gastropathy and to understand the factors causing the delay of gastric emptying time in patients with cirrhosis. Methods: Between April 1996 and September 1996, 30 cirrhotic patients with congestive gastropathy, 27 cirrhotic patients without congestive gastropathy, and 15 controls were participated in this study at St, Mary Hospital. All patients were subjected to evaluation of gastrointestinal symptom score, endoscopy, Child-Pugh score, and gastric emptying time with solid meal. Results: The gastric emptying time was longer in cirrhotic patients with congestive gastropathy than in controls (105.6±9.58 vs 72.4±6.02 minutes), but there was no significant difference between cirrhotic patierits without congestive gastropathy and controls (93.7±7.93 vs 72.4±6.02 minutes). Among Factors influencing gastric emptying time in cirrhosis such as congestive gastropathy, ascites, gastric varix, AST, ALT, Child-Pugh score, prothrombin time, age, albumin level, esophageal varix, and bilirubin, only the congestive gastropathy was statistically significant (p$lt;0.05). Conclusions: The gastric emptying time was delayed in cirrhotic patients who had congestive gastropathy. Thus, congestive gastropathy may contribute to the pathogenesis of delayed gastric emptying in cirrhosis.