Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

Managing Cultural Diversity by Effective Human Resource Management of International Trade: Focus on Asian Perspectives

Shin,Soo Yong,Pak,Myong Sop,Cho,Sung Woo 한국무역상무학회 2012 貿易商務硏究 Vol.56 No.-

Changes in the global business environment continue at an accelerated pace. The 21st century maybe remarked, perhaps, for its hot issues on globalization and diversity. Diversity may arise in terms of ethnic, gender, age and culture. Cultural diversity out of all stands out in front in modern times as more multinational companies operate internationally resulting more people to start living abroad and work with people from different cultures. In recent years, these multinational companies realized the important roles human resource management play in international trade since cultural diversity is very influential on their work performance. A well-managed human resources will ensure organizations' success to a great extent. This paper touches the field of cultural diversity and introduces human resource management of international trade as a way to handle diversity matters faced by international organizations.

깁스표본기법을 이용한 와이블분포의 위험률에 대한 베이즈추정

조건호,이우동,신양규,윤용화 慶山大學校 基礎科學硏究所 1998 基礎科學 Vol.2 No.1

와이블분포를 따르는 표본을 이용하여 위험률을 베이지안 방법을 이용하여 추정한다. 깁스표본법을 베이지안 추정에서 사후확률의 분포를 구하는 방법에 적용하여 위험률에 대한 사후분포와 주변사후분포를 추정하는 방법을 제안한다. 그리고, 9개의 가솔린 정류에서 쓴 sdufwjsekfrlrn(heat exchanger)에 대한 수명시간자료를 이용하여 제안된 방법으로 위험률에 대한 베이즈추정량을 구하고 최우추정량과 비교한다. In this paper, a Bayesian estimation of the hazard rate function of Weibull distribution is considered. Using Gibbs sampling approach, the posterior distribution of hazard and Bayes estimator of hazard are presented. Using 9 heat exchanger data, the proposed method is illustrated.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

강직성 슬관절에서의 슬관절 전치환술

조우신,박종희,김정민,오세관 대한슬관절학회 2003 대한슬관절학회지 Vol.15 No.1

목적: 강직이 동반된 슬관절염 환자에서 슬관절 전치활술의 문제점, 결과 및 합병증을 분석하고자 하였다. 대상 및 방법: 1994년 1월부터 2000년 12월까지 슬관절 강직(운동 범위 50도이하)이 있는 환자에게 슬관절 전치환술을 시행하여 2년 이상 추시가 가능하였던 16례를 대상으로 하였다. 수술은 관절 강직이 발생한지 평균 12.2년(1~40년)에 시행하였으며, 추시기간은 평균 3.3년(2~6년)이었다. 16례 중 남자 3례, 여자 13례였고, 평균 나이는 55.8세(34세~75세)였다. 감염에 의한 후유증 6례로 가장 많았고 그 외 퇴행성 관절염 4례, 외상 후 관절염 3례, 류마티스성 관절염 3례의 순이었다. 술 후 평가는 추시기간 중의 운동 범위와 HSS score 및 합병증을 분석하였다. 결과: 관절 운동 범위는 술 전 평균 31.6도(0~50도)에서 추시시 평균 95.4도(80~120도)로 증가하였으며, 1례에서만 5도의 굴곡 구축이 있었다. HSS score는 술 전 평균 59.6점(43~76점)에서 추시시 평균 84.6점(76~92점)으로 향상되었다. 1례에서 수술 중 슬개건 부분파열이 있었으나 보조기 착용과 적극적 재활로 술 후 1년째 90°의 관절 운동이 가능했다. 표재성 감염 1례는 창상의 변연절제술 후 피부이식으로 치유되었고, 심부 감염된 3례는 추후 관절 유합술을 시행하였다. 결론: 슬관절 전지환슬은 강직된 슬관절의 기능을 회복시킬 수 있는 좋은 술기이지만 감염등의 합병증을 고려해서 시행되어야 한다. Purpose: To evlauate the results of TKRA (Total knee replacement arthroplasty) for the treatment of stiff knee. Methods & Materials : TRKA has been performed for 18 cases of stiff knee ( ROM < 50° ) between January 1994 and December 2000. 16 cases which have been followed up for more than 2 years were analysed. Average follow-up was 3.3 years(2~6 years). 3 were male and 13 female. The average age was 55.8 years(34~75 years). Sequales of infection were most with 6 cases and followed by osteoarthritis (4 cases, 2 cases had been performed arthroscopic debridement) and rheumatoid arthritis (3 cases) and traumatic arthritis (3 cases) in order. TKRA was performed on average 12.2 years (1~40 years) after knee stiffness has been developed. We evaluate the results with ROM, HSS score and complications. Results : Average ROM was increased from 31.6°(0°~50°) preoperatively to 95.4°(80°~120°) postoperatively. 5° of flexion contracture was present in only 1 cases. Average HSS score was improved from 59.6 points preoperatively to 84.6 points postoperatively. The patella tendon was partially ruptured in 1 case surgery, but by brace application and rehabilitative exercise, ROM was improved to 90°, 1 year post operatively. There were 1 superficial skin infection which was resolved by revision of wound and skin graft, and deep infection in 3 cases, which needed knee fusion finally. Conclusion : TKRA is a good method for improving function in knee stiffness although infection risk is high.

나노크리스탈 약물 전달 기술

황우신,황성주,박정숙,조정원 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

Nanotechnology affects on various aspects including medicine and pharmaceutics. This nanocrystal technology which transforms to nano-sized material is able to develop the innovational formulation strategy for poorly water-soluble drugs due to the change of physicochemical properties of drug. Scale-up methods using pearl milling or high pressure homogenizer was described and commercialized products based on nanotechnology were introduced in this manuscript.

珪酸마그네슘의 合成條件에 關한 硏究

전우정,신화우,최광식,안세민,이광표,장영수 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-

Magesium twisilicate is mainly uaed as a nonsytemic antacid andabsrbent. Magnesium trisilicate when teeated with anacid sxuh as diluted hydrochloric acid in gastric juice decomposes to form Magnesium chloride and colloidal Silicates. 2MgO·3SiO_2 + 4Hcl - 2MgCl_2 + H_4Si_3O_8 (or 3SiO_2 + 2H_2O) Colloidal mixture The resultng colloidal silicates can protect ahe ulcer frdm further acid and peptic attack and possibly adsorb the pepsin. It is well known that yield of Magnesium trisilcate produced is greatly affected by the Synthesis condition such as the reactant concentration, reaction temperature and moleatio of reactant solutions as well as by the emperature at which the precipitate is dried and the temperature of washing water, etc. The purpose of this study is to investigate the optimum synthesis conditions of Magnesium trisilicate. A randomized complete block design suggested by G.E.P. Bdx and K.B. Wagnesium sulfate solution with Sodium silicate solution in this study. It was found that optimal synthesis conditidns were: Reaction temperature range : 57-90 ℃. Concentration range of reactants (Sodium silicate and Magnesium sulfate) : 19.1-29% molar ratio of the reactants. [Sodium silicate]/[Magnesium sulfate]: 1.47-1.80. temperature range of washing water: 45-48℃ and drying temperature range: 65-82℃ The antacidic activity of five Magnesium trisilicate samples which shows the maximum antacidic efficacy was tested by pharmacopeia acid consuming capacity test. The five Magnesium trisilicate samples were samples were identified by chemical analysis.

보건소 진료서비스 이용자의 만족도에 관한 연구

문영신,조우현,강임옥 한국의료QA학회 1998 한국의료질향상학회지 Vol.5 No.1

The aim of this study was to examine differences in the level of satisfaction with medical care at a public health center between patients receiving free-care compared to those paying out-of-pocket. SERVQUAL(Comprehensive Service Quality Measurement Scale), a pyschometrically valid measure for evaluating hospital service quality, was used to assess patint satisfaction. The study sample consisted of 279 patients who received medical care at a public health center. The interview was conducted between April 23 and May 7. 1998. The results was as follow: 1. There were more elderly people among free-care patients than out-of-pocket patients. Education and income levels were lower amongs those -receiving free-care. There 'were however, no differences in the distribution of gender and religion between the two groups. Patients receiving free-care tended to visit the public health center more frequently compared to those self-paying patients. 2. Overall, free-care patients showed higher satisfaction level than that of self-paying patients, except for the dimension on sympathy and shape.

당뇨병성 신증 환자 20예에서 복합한약전탕액이 신기능에 미치는 영향

최우정,김동웅,신선호,이언정,최진영,신학수,조권일,이광석 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2

Diabetic Nephropathy is one of the major causes of chronic renal failure. It is a common microvascular complication and clinically defined as the presence of persistent Proteinuria. We studied the effects and change of the renal function of Complex Herbal medication of the 20Diabetic Nephropathy patients. We measured the initial levels of Total Protein, Creatinine Clearance Rate(Ccr), Serum Creatinine(Serum-Cr), Urine Creatinine(Urine-Cr) and HbA1C on admission and followed up the level changes of Total Protein, Ccr, Serum-Cr and Urine-Cr on discharge. The results are following : Complex Herbal Medication does not cause the renal toxicity. The longer hypertension period is, the higher Serum-Cr level and Urine-Cr level. In an older age group, Urine-Cr is lower. 4.From the "Deficiency in Origin and Excess in Superficiality(本虛表實)“points of view, Complex Herbal Medication improves the Serum-Cr in Diabetic Nephropathy patients. According to this results, it could be suggested that Complex Herbal Medication does not cause the renal toxicity in Diabetic Nephropathy patients and intensive controls of blood sugar, blood pressure and Complex Herbal Medication prevent the renal failure in Diabetic Nephropathy patients with early stage of Microalbumiuria.

황정(黃精) 추출물의 화학구조결정에 관한 연구(Ⅱ)

김흥재,조수동,권기락,안철진,주우홍,강진호,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1

본 연구에서는 황정 속에 포함되어 있는 생리활성 물질을 hexane, CHCl₃과 n-butanol층에서 각각의 성분들을 추출하였고, hexane층에서 분리된 화합물 I과 Ⅱ 중에서, 화합물 I의 봐학구조를 ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC 스펙트럼 및 MS 스펙트럼 등의 분광학적인 방법에 의해 결정하였다. 화합물 I의 구조는 9,12-(9E,12E) -octadecadienoyloxy) propanoic acid 임을 확인하였다. In this Paper, biologically active compounds were extracted using organic solvents as hexane. CHC1₃, n-butanol to give each component. Chemical structure of compound I was characterized using ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC spectrum and MS-spectrum, in separated compound I and Ⅱ from hexane layer. Finally, chemical structure of compound I was determined as 9,12-(9E,12E)-octadecadienoyloxy)propanic acid.