Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Phase I and pharmacokinetic study of cisplatin and troxacitabine administered intravenously every 28 days in patients with advanced solid malignancies

Lin, Chia-Chi,Beeram, Muralidhar,Rowinsky, Eric K.,Takimoto, Chris H.,Ng, Chee M.,Geyer Jr, Charles E.,Denis, Louis J.,De Bono, Johann S.,Hao, Desiree,Tolcher, Anthony W.,Rha, Sun-Young,Jolivet, Jacqu Springer-Verlag 2009 Cancer chemotherapy and pharmacology Vol.65 No.1

Platelet-Derived Growth Factor Receptor-α Subunit Targeting Suppresses Metastasis in Advanced Thyroid Cancer In Vitro and In Vivo

( Ching-ling Lin ),( Ming-lin Tsai ),( Yu-hsin Chen ),( Wei-ni Liu ),( Chun-yu Lin ),( Kai-wen Hsu ),( Chien-yu Huang ),( Yu-jia Chang ),( Po-li Wei ),( Shu-huey Chen ),( Li-chi Huang ),( Chia-hwa Lee 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.5

Thyroid cancer is the most common endocrine malignancy. Patients with well-differentiated thyroid cancers, such as papillary and follicular cancers, have a favorable prognosis. However, poorly differentiated thyroid cancers, such as medullary, squamous and anaplastic advanced thyroid cancers, are very aggressive and insensitive to radioiodine treatment. Thus, novel therapies that attenuate metastasis are urgently needed. We found that both PDGFC and PDGFRA are predominantly expressed in thyroid cancers and that the survival rate is significantly lower in patients with high PDGFRA expression. This finding indicates the important role of PDGF/PDGFR signaling in thyroid cancer development. Next, we established a SW579 squamous thyroid cancer cell line with 95.6% PDGFRA gene insertion and deletions (indels) through CRISPR/Cas9. Protein and invasion analysis showed a dramatic loss in EMT marker expression and metastatic ability. Furthermore, xenograft tumors derived from PDGFRA geneedited SW579 cells exhibited a minor decrease in tumor growth. However, distant lung metastasis was completely abolished upon PDGFRA gene editing, implying that PDGFRA could be an effective target to inhibit distant metastasis in advanced thyroid cancers. To translate this finding to the clinic, we used the most relevant multikinase inhibitor, imatinib, to inhibit PDGFRA signaling. The results showed that imatinib significantly suppressed cell growth, induced cell cycle arrest and cell death in SW579 cells. Our developed noninvasive apoptosis detection sensor (NIADS) indicated that imatinib induced cell apoptosis through caspase-3 activation. In conclusion, we believe that developing a specific and selective targeted therapy for PDGFRA would effectively suppress PDGFRA-mediated cancer aggressiveness in advanced thyroid cancers.

Prediction of the Duration to Next Admission for an Acute Affective Episode in Patients with Bipolar I Disorder

Pao-Huan Chen(Pao-Huan Chen),Chun-Ming Shih(Chun-Ming Shih),Chi-Kang Chang(Chi-Kang Chang),Chia-Pei Lin(Chia-Pei Lin),Yung-Han Chang(Yung-Han Chang),Hsin-Chien Lee(Hsin-Chien Lee),El-Wui Loh(El-Wui Lo 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.2

Objective: Predicting disease relapse and early intervention could reduce symptom severity. We attempted to identify potential indicators that predict the duration to next admission for an acute affective episode in patients with bipolar I disorder. Methods: We mathematically defined the duration to next psychiatric admission and performed single-variate regressions using historical data of 101 patients with bipolar I disorder to screen for potential variables for further multivariate regressions. Results: Age of onset, total psychiatric admissions, length of lithium use, and carbamazepine use during the psychiatric hospitalization contributed to the next psychiatric admission duration positively. The all-in-one found that hyperlipidemia during the psychiatric hospitalization demonstrated a negative contribution to the duration to next psychiatric admission; the last duration to psychiatric admission, lithium and carbamazepine uses during the psychiatric hospitalization, and heart rate on the discharge day positively contributed to the duration to next admission. Conclusion: We identified essential variables that may predict the duration of bipolar I patients’ next psychiatric admission. The correlation of a faster heartbeat and a normal lipid profile in delaying the next onset highlights the importance of managing these parameters when treating bipolar I disorder.

다수제적 혼합선거제도 하에서의 투표행태에 대한 다차원 분석

( Chia Hung Tsai ),( Lu Huei Chen ),( Chao Chi Lin ),( Su Feng Cheng ) 한국선거학회 2011 선거연구 Vol.1 No.2

혼합선거제도는 영국의 전통적 소선거구제와 비례제 양자 모두의 좋은 점을 제공한다고 언급된다. 학자들은 후보자와 정당들이 이러한 선거 규칙에 어떻게 반응하는지 고찰해 왔으며, 이 제도 하에서는 상이한 유인 요인이 존재한다고 주장해 왔다. 두 개의 서로 다른 층 간의 상호작용 또한 연구의 대상이 되어 왔다. 본 논문은 소선거구제에서의 투표 행태가 정당의 명성에 의해 영향 받는 정도를 평가해 보고자 하는데, 이는 소선거구 선거 결과와 비례대표 선거 결과 간의 차이에 의해서 측정된다. 대부분의 유권자가 비례대표 표를 던질 때 자신들의 선호를 따르며, 소선거구 표를 던질 때는 현직자를 고려할 것이라고 우리는 가정한다. 한국, 대만, 그리고 일본에서의 투표 행태를 비교함으로써, 사회적 배경, 정치적 태도, 쟁점에 대한 의견 등이 투표 행태에 미치는 상대적 영향력을 이들 세 국가에 걸쳐서 평가할 수 있다. 분석에 사용되는 자료는 선거제도 비교연구(CSES) 2차조사 (2001-2006), 21세기 전국적 장기 설문조사 자료 (JES 3차), 대만의 선거 및 민주화 연구 자료 (TEDS 2008L)이며, 이를 통해 각기 한국의 2004년 총선, 대만의 2008년 의회선거, 그리고 일본의 총선을 고찰한다. It is said that mixed-member systems offer the best of both the traditional British single-member district system (SMD) and proportional representation systems (PR). Scholars have examined how candidates and parties respond to the electoral rule, arguing that there are different incentives for them. The interaction between the two tiers is also investigated. This paper attempts to assess the extent to which voting behavior in the SMD districts would be influenced by party reputation measured by the difference between the SMD and PR voting results. We assume that most voters follow their preferences when they cast their PR votes, but that they consider the incumbents when they cast their SMD ballots. Comparing voting behavior in Korea, Taiwan, and Japan, we are able to assess the relative effect of social backgrounds, political predispositions, and issue opinions on voting behavior across three countries. We use CSES Module 2 (2001-2006) data, the Nation-wide Longitudinal Survey Study on Voting Behavior in the 21st Century (JES III) data, and Taiwan`s Election and Democratization Study (TEDS 2008L) data to examine voting behavior in the 2004 National Assembly election of Korea, the 2008 legislative election of Taiwan, and the 2005 general election of Japan respectively.

Human-Oriented Recognition for Intelligent Interactive Office Robot

Chia-Ming Wang,Shin-Huan Tseng,Pei-Wen Wu,Yuan-Han Xu,Chien-Ke Liao,Yu-Chi Lin,Yi-Shiu Chiang,Chung-Dial Lim,Ting-Sheng Chu,Li-Chen Fu 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

This paper presents our new intelligent interactive robot, which is constructed to eagerly provide multi-functional services in an office environment. In order to endow a full interactive capability of our robots for realizing so-called human-robot interaction (HRI), we propose sensor fusion based human detection and tracking system and human pose estimation to deal with a number of situations which may take place in the office environment. Not only by these perceptions, human interact with the robot also by some natural way, such as touching the interface screen and talking with the robot through microphone. Finally, the effectiveness of the proposed work is tested and validated by some of experiments.

Characteristics of cross-flow rotating packed beds

Chia-Chang Lin,Bor-Chi Chen 한국공업화학회 2008 Journal of Industrial and Engineering Chemistry Vol.14 No.3

This work examined pressure drop and mass transfer of the cross-flow rotating packed bed (RPB) using absorption of carbon dioxide (CO₂) from gas. As to pressure drop, the gas flow rate was observed to be a more important factor than the rotor speed and the liquid flow rate due to less liquid holdup in the cross-flow RPB. The overall volumetric gas-phase mass transfer coefficients (KGα) were determined as a function of the rotor speed, the gas flow rate and the liquid flow rate. The dependences of KGα on the gas flow rate and the liquid flow rate indicated that the resistance to mass transfer in the liquid phase for CO₂ absorption was higher than that in the gas phase in the cross-flow RPB. Moreover, the obtained results demonstrated that the KGa values of the cross-flow RPB were comparable to those of the countercurrent-flow RPB, suggesting a great potential of the cross-flow RPB applied to the removal of CO₂ from gaseous streams.

Carbon Dioxide Absorption into NaOH Solution in a Cross-flow Rotating Packed Bed

Chia-Chang Lin,Bor-Chi Chen 한국공업화학회 2007 Journal of Industrial and Engineering Chemistry Vol.13 No.7

A cross-flow rotating packed bed (RPB) was applied to absorb carbon dioxide (CO2) by NaOH solution from gaseous streams. The cross-flow RPB has an inner radius of 2.4 cm, an outer radius of 4.4 cm and an axial height of 12.0 cm. Wire mesh was used as packings. Rotor speeds ranged from 540 to 1600 rpm, providing 11∼97 equivalent gravitational force. The removal efficiency of CO2 was determined at various values of operating parameters, including the rotor speed, gas flow rate, liquid flow rate, and NaOH concentration. Our experimental results demonstrated that the removal efficiency of CO2 increased with the rotor speed, liquid flow rate and NaOH concentration; however, decreased with the gas flow rate. Furthermore, a cross-flow RPB provides a reduction in the dosage of the NaOH solution for CO2 absorption, suggesting that a cross-flow RPB has a great potential in the removal of CO2 from the exhausted gases.

Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: implication for human lymphocyte proliferation

Chia-Eng Wu,Chen-Wei Yu,Kai-Wei Chang,Wen-Hsi Chou,Chen-Yu Lu,Elisa Ghelfi,Fang-Chun Wu,Pey-Shynan Jan,Mei-Chi Huang,Patrick Allard,Shau-Ping Lin,Hong-Nerng Ho,Hsin-Fu Chen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), represent potentially unlimited cell sources for clinical applications. Previous studies have suggested that hPSCs may benefit from immune privilege and limited immunogenicity, as reflected by the reduced expression of major histocompatibility complex class-related molecules. Here we investigated the global immune-related gene expression profiles of human ESCs, hiPSCs and somatic cells and identified candidate immune-related genes that may alter their immunogenicity. The expression levels of global immune-related genes were determined by comparing undifferentiated and differentiated stem cells and three types of human somatic cells: dermal papilla cells, ovarian granulosa cells and foreskin fibroblast cells. We identified the differentially expressed genes CD24, GATA3, PROM1, THBS2, LY96, IFIT3, CXCR4, IL1R1, FGFR3, IDO1 and KDR, which overlapped with selected immune-related gene lists. In further analyses, mammalian target of rapamycin complex (mTOR) signaling was investigated in the differentiated stem cells following treatment with rapamycin and lentiviral transduction with specific short-hairpin RNAs. We found that the inhibition of mTOR signal pathways significantly downregulated the immunogenicity of differentiated stem cells. We also tested the immune responses induced in differentiated stem cells by mixed lymphocyte reactions. We found that CD24- and GATA3-deficient differentiated stem cells including neural lineage cells had limited abilities to activate human lymphocytes. By analyzing the transcriptome signature of immune-related genes, we observed a tendency of the hPSCs to differentiate toward an immune cell phenotype. Taken together, these data identify candidate immune-related genes that might constitute valuable targets for clinical applications.

Delay of Surgery for Spinal Metastasis due to the COVID-19 Outbreak Affected Patient Outcomes

Chia-Jung Hsieh,Chun-Yu Wu,Yen-Heng Lin,Yu-Cheng Huang,Wen-Chi Yang,Tom Wei-Wu Chen,Wei-Li Ma,Wei-Hsin Lin,Feng-Ming Hsu,Furen Xiao,Shih-Hung Yang,Dar-Ming Lai,Chang-Mu Chen,Shin-Yi Chao,Fon-Yih Tsuan 대한척추신경외과학회 2023 Neurospine Vol.20 No.4

Objective: The present study is to analyze the effects of the coronavirus disease 2019 (COVID 2019) outbreak and the subsequent lockdown on the outcomes of spinal metastasis patients. Methods: The study was a retrospective analysis of data from a prospective cohort study. All patients underwent surgical intervention for spinal metastases between January 2019 and December 2021 and had at least 3 months of postoperative follow-up. The primary outcome was overall mortality during the 4 different stages (pre-COVID-19 era, COVID-19 pandemic except in Taiwan, national lockdown, lifting of the lockdown). The secondary outcomes were the oncological severity scores, medical/surgical accessibility, and patient functional outcome during the 4 periods as well as survival/mortality. Results: A total of 233 patients were included. The overall mortality rate was 41.20%. During the Taiwan lockdown, more patients received palliative surgery than other surgical methods, and no total en bloc spondylectomy was performed. The time from surgeon visit to operation was approximately doubled after the COVID-19 outbreak in Taiwan (75.97, 86.63, 168.79, and 166.91 hours in the 4 periods, respectively). The estimated survival probability was highest after the national lockdown was lifted and lowest during the lockdown. In the multivariate analysis, increased risk of mortality was observed with delay of surgery, with emergency surgery having a higher risk with delays above 33 hours, urgent surgery (below 59 and above 111 hours), and elective surgery (above 332 hours). Conclusion: The COVID-19 pandemic and related policies have altered daily clinical practice and negatively impacted the survival of patients with spinal metastases.