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Li Hong,Chengliang Yan,Tang Yanqiong,Ma Xiang,Chen Yinhua,Chen Songbi,Lin Min,Liu Zhu 한국미생물학회 2020 The journal of microbiology Vol.58 No.7
Endophytes colonize tissues of healthy host plants and play a crucial role in plant growth and development. However, little attention has been paid to the endophytes of tuber crops such as cassava, which is used as a staple food by approximately 800 million people worldwide. This study aimed to elucidate the diversity and composition of endophytic bacterial and fungal communities in different cassava cultivars using high-throughput sequencing. Although no significant differences in richness or diversity were observed among the different cassava cultivars, the community compositions were diverse. Two cultivars (SC124 and SC205) tolerant to root rot exhibited similar community compositions, while two other cultivars (SC10 and SC5), which are moderately and highly susceptible to root rot, respectively, harboured similar community compositions. Proteobacteria, Firmicutes, and Ascomycota dominated the endophyte assemblages, with Weissella, Serratia, Lasiodiplodia, Fusarium, and Diaporthe being the predominant genera. The differentially abundant taxonomic clades between the tolerant and susceptible cultivars were mainly rare taxa, such as Lachnoclostridium_5, Rhizobium, Lampropedia, and Stenotrophomonas. These seemed to be key genera that affected the susceptibility of cassava to root rot. Moreover, the comparison of KEGG functional profiles revealed that ‘Environmental adaptation’ category was significantly enriched in the tolerant cultivars, while ‘Infectious diseases: Parasitic’ category was significantly enriched in the susceptible cultivars. The present findings open opportunities for further studies on the roles of endophytes in the susceptibility of plants to diseases.
The Functional Properties of Preserved Eggs: From Anti-cancer and Anti-inflammatory Aspects
Changyi Mao,Zhihui Yu,Chengliang Li,Yongguo Jin,Meihu Ma 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.3
Preserved egg, a kind of alkaline-fermented food, is a traditional egg product in China. Here, we investigated the nutritional functions of preserved eggs by in vivo and in vitro experiments. The results of in vivo studies showed that the levels of triglycerides (TG), total cholesterol (TCHO) and low-density lipoprotein cholesterol/high density lipoprotein cholesterol (LDL-C/HDL-C) were significantly decreased (p<0.05) in the liver of rats treated with preserved eggs. Meanwhile, the levels of two important cancer markers, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), were also significantly decreased (p<0.05) in treated rats. In vitro studies were performed on Caco-2 cells, a human epithelial colorectal adenocarcinoma cell line. It demonstrated that the gastrointestinal (GI) digests of preserved eggs significantly accelerated (p<0.05) the apoptosis by upregulating caspase-3 in the Caco-2 cells. Besides, after treated with preserved eggs, the half maximal inhibitory concentration (IC50) of preserved eggs digests to Caco-2 cells was 5.75 mg/mL, indicating the significant inhibition of cell proliferation provided by preserved eggs (p<0.05). The results shown in this study demonstrated that preserved eggs may be a novel functional food involved with antilipemic, anti-inflammatory activity as well as the effect on accelarating the apoptosis of Caco-2 cells.
Shenggang Fan,Runmin Ding,Shaoru Zeng,Chenxu Li,Chengliang Liu 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.6
The steel plate shear wall (SPSW) with two-side connections can be arranged fl exibly in the structure, and has no additional infl uence on the column, but its buckling capacity is diffi cult to be deduced directly by thin plate theory. The low yield point SPSW (LYP-SPSW) with two-side connections is proposed in this paper, including unstiff ened LYP-SPSW, cross-stiff ened LYP-SPSW and diagonally-stiff ened LYP-SPSW. The elastic buckling performance and buckling capacity of these three types of LYP-SPSWs are studied by numerical simulation. On the basis of the accurate model, a series of parametric analysis on the key factors were conducted, including the height-to-thickness ratio, the stiff ness ratio of the stiff ener, and the stiff ness ratio of the edge member, which have eff ects on the elastic buckling stress of the LYP-SPSW with two-side connections. The elastic buckling stress of the SPSW can be eff ectively increased by adding stiff eners and edge members, but it is unnecessary to use oversized stiff eners and edge members. Based on a large amount of parametric analysis, simplifi ed formulas for calculating the elastic buckling stresses of the three types of LYP-SPSWs mentioned above are proposed in this paper.
Yaqi Suo,Shenggang Fan,Chenxu Li,Shaoru Zeng,Chengliang Liu 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.6
Parametric analysis on the hysteresis performance of the low yield point steel plate sheer wall (LYP SPSW) with three different stiff ening confi gurations, including unstiff ened, cross stiff ened and diagonal stiff ened, was conducted using the fi nite element models which have been verifi ed by the test results. The parameters which have eff ects on the hysteretic behavior of LYP SPSW include ratio of height-to-thickness, stiff ness ratio of stiff ener, and the stiff ness ratio of the edge member. The hysteretic curves, skeleton curves and viscous damping coeffi cient-displacement curves of SPSW with diff erent stiff ening confi gurations were obtained. According to the numerical results, it can be seen that the shear resistance and hysteretic performance of LYP SPSW decrease with the increase of the ratio of height-to-thickness. The energy dissipation capacity and shear capacity of LYP SPSW can be improved eff ectively by setting cross and diagonal stiff eners, but when the stiff - ness ratio of stiff ener is greater than 10, the increase is no longer obvious. Furthermore, for steel plate shear walls without edge members, the diagonal cross-stiff ening steel shear wall can achieve better energy consumption. For steel plate shear walls with edge members, when the ratio of height-to-thickness is less than 100, a better energy dissipation can be achieved by using cross stiff ened steel plates; when the ratio of height-to-thickness is greater than or equal to 200, a better energy dissipation can be refl ected by adopting cross stiff ened steel plates. Finally, a restoring force model of the LYP SPSW with two-side connections is proposed.
Association of Apolipoprotein E Polymorphisms with White Matter Lesions and Brain Atrophy
ZhiLi Niu,PingAn Zhang,Dong Li,ChengLiang Zhu,LiNa Feng,Ge Xiong,NaNa Song,Pei Tang,Feng Liu 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.2
Objective Apolipoprotein E (ApoE) is mainly synthesized in the liver. So far, it is unknown the relationship among APOE gene polymorphisms and WML, brain atrophy. Therefore, the aim of the study was to assess the associations of APOE gene polymorphisms in patients with WML and brain atrophy. Methods A total of 58 patients with WML, 128 patients with brain atrophy, 112 patients with co-occurrence of WML and brain atrophy and 95 healthy elderly volunteers were recruited from Renmin Hospital of WuHan University. Results Allele E3 was the most common allele. The alleles E2 had significantly higher levels of ApoB and lower age in WML group. The alleles E2 was associated with the lower level of ApoB, LDL-Ch, TCh, and sdLDL in co-occurrence group. The E3/E3 genotype has higher level of sdLDL, but lower age and female frequency in WML. The E3/E4 genotype had higher level of TG, but lower age in WML. Gender, Age, E2, Hyperhomocysteinemia and UA were also significantly associated with disease progression. Conclusion This study found that clinical data, lipids and metabolic complications were closely related to ApoE genotypes and alleles, and also disease progression and type.
Zhiwei Xue,Shemin Zhu,Yuesong Shen,Shubao Shen,Chengliang Li 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.30 No.-
Promotional effects of Ce4+, La3+ and Nd3+ incorporations on FeCu2O3.5/ATS for decomposing N2O wereinvestigated. Results showed that all the ions especially La3+ can dramatically improve the catalyticperformance. FeCu2La1.5Ox/ATS, the optimal catalyst, can decompose 100% N2O at 550 8C. The formedperovskite-type LaFeO3 and spinel-type CuLa2O4 largely improved catalytic performance. The ionmodification increased N2O adsorption capacity, and La3+-doped catalyst enhanced desorption ability ofsurface oxygen. Both effects can accelerate the rate-determine steps of N2O decomposition reaction,promote catalytic performance. Moreover, the supported catalysts studied were economic efficiency forapplication.