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Yongjie Chen,Ji Xu,Chao Yang,Hongyu Zhang,Feng Wu,Jian Chen,Kai Li,Hailong Wang,Yu Li,Yinghui Li,Zhongquan Dai 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Long-term spaceflight affects numerous organ systems in the body, including metabolic dysfunction. Recently, ample evidence has demonstrated that the liver is a vulnerable organ during spaceflight. However, the changes in hepatocyte proliferation and cell cycle control under microgravity remain largely unexplored. In the present study, we first confirmed that the serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase, biochemical markers of liver function, were altered in rats under tail suspension (TS) conditions to simulate microgravity, as shown in previous reports. Next, we demonstrated that the cell proliferation activity, determined by Ki67, PCNA and PH3, was significantly decreased at the different TS time points (TS for 14, 28 and 42 days) compared with that in the control group. Consistently, the positive cell cycle regulators Ccna2, Ccnd1, Cdk1, Cdk2 and cyclin D3 were also significantly decreased in the TS groups as shown by quantitative real-time PCR and western blotting analysis. Subsequent analysis revealed that the aberrant hepatocyte proliferation inhibition under simulated microgravity was associated with the upregulation of miR-223 in the liver. We further found that miR-223 inhibited the proliferation of Hepa1-6 cells and identified CDK2 and CUL1 as its direct targets. In addition, the decreased expression of CDK2 and CUL1 was negatively correlated with the level of p27 in vitro and in vivo, which may have been responsible for retarding hepatocyte proliferation. Collectively, these data indicate that upregulation of miR-223 was associated with the inhibition of liver cell growth and reveal the role of miR-223 in rat hepatocyte proliferation disorders and the pathophysiological process under simulated microgravity.
Attribute-Based Data Sharing with Flexible and Direct Revocation in Cloud Computing
( Yinghui Zhang ),( Xiaofeng Chen ),( Jin Li ),( Hui Li ),( Fenghua Li ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.11
Attribute-based encryption (ABE) is a promising cryptographic primitive for implementing fine-grained data sharing in cloud computing. However, before ABE can be widely deployed in practical cloud storage systems, a challenging issue with regard to attributes and user revocation has to be addressed. To our knowledge, most of the existing ABE schemes fail to support flexible and direct revocation owing to the burdensome update of attribute secret keys and all the ciphertexts. Aiming at tackling the challenge above, we formalize the notion of ciphertext-policy ABE supporting flexible and direct revocation (FDR-CP-ABE), and present a concrete construction. The proposed scheme supports direct attribute and user revocation. To achieve this goal, we introduce an auxiliary function to determine the ciphertexts involved in revocation events, and then only update these involved ciphertexts by adopting the technique of broadcast encryption. Furthermore, our construction is proven secure in the standard model. Theoretical analysis and experimental results indicate that FDR-CP-ABE outperforms the previous revocation-related methods.
Zhang Chen,Su Yongbo,Mei Bo,Yang Feng,Zhang Jialin,Yun Huanqing,Liu Bo,Sun Yi,Zhang Haiming,Jin Zhi,Zhong Yinghui 한국물리학회 2023 Current Applied Physics Vol.48 No.-
InP-based hetero-junction bipolar transistors (HBTs) are attractive for various millimeter-wave and terahertz electronics due to their ultrahigh frequency performance. Therefore, the study of their irradiation reliability is extremely urgent. In this work, 2 MeV proton irradiation experiment has been carried out on self-fabricated N+Np+ InP/InGaAs hetero-junction. The degradation mechanisms have systematically been studied after exposure to protons of 5 × 1013 H+/cm2. The voltage range of recombination current at low forward bias has been largely expanded from 0-0.2 V to 0–0.6 V. Furthermore, the interface states properties have been characterized by utilizing the frequency-dependent conductance technique, the interface states density (Dit) has increased from approximately 9.02 × 1012 cm-2 eV-1–1.99 × 1013 cm-2 eV-1 to 9.51 × 1012 cm-2 eV-1–2.00 × 1013 cm-2 eV-1 after irradiation. The study would be great significance for understanding the evolution of proton irradiation on relative devices.
Wang Yinghui,Zhang Yanan,Wang Jiangfeng,Kang Chengtao,Hu Gengwang,Guo Yi,Chen Jie,Yang Lijun,Wang Yu 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), isomnivorous andwidely distributed in the world. Black soldier flies usually colonize corpse at a relatively late stage and have a longer developmental time compared to other flies. It can serve as a good supplementary indicator for estimating a longer minimum postmortem interval (PMI min ) in forensic investigations. In this paper, the development of H. illucens was investigated at six constant temperatures between 19 and 34 ◦ C. Under temperatures of 19, 22, 25, 28, 31, and 34 ◦ C, the mean developmental time from eggs to adults was 90.96, 58.29, 54.61, 42.33, 32.33 and 34.52 days, respectively. Developmental time and accumulated degree days results were used to construct thermal sum mation model and isomorphen diagram. The calculated values of lower developmental thresholds and thermal summation constant estimated by a linear model were 11.88 ◦ C and 650.89 degree days, respectively. Through a nonlinear model, the upper lethal developmental threshold temperature, intrinsic optimum temperature and lower developmental thresholds were estimated to be 35.18, 20.50 and 13.17 ◦ C, respectively. A growth curve, an equation and isomegalen diagram based on them show the relationship between developmental time and larval body length. The larval body length increased with increased developmental time and eventually pla teaued, revealing an S-shape growth curve. These results provide important basic developmental data for H. illucens, which can be used to estimate the PMI min .
Defense responses of sulfur dioxygenase to sulfide stress in the razor clam Sinonovacula constricta
Caifang Chen,Yaoyao Shen,Weiliang Shen,Jing He,Zhihua Lin,Yinghui Dong 한국유전학회 2021 Genes & Genomics Vol.43 No.5
Background Sulfde is a well-known toxicant widely distributed in the culture environment. As a representative burrowing benthic bivalve, the razor clam Sinonovacula constricta is highly sulfde tolerant. Mitochondrial sulfde oxidation is an important way for sulfde detoxifcation, where sulfur dioxygenase (SDO) is the second key enzyme. Objective To investigate the mechanism of sulfde tolerance in S. constricta, the molecular characterization of its SDO (designated as ScSDO) was studied. Methods The cDNA sequence of ScSDO was cloned by RACE technique. The response of ScSDO in gills and livers of S. constricta was investigated during sulfde exposure (50, 150, and 300 μM sulfde) for 0, 3, 6, 12, 24, 48, 72, and 96 h by qRTPCR. Moreover, the temporal expression of ScSDO protein in S. constricta gills after exposure to 150 μM sulfde was detected by Western blot. The subcellular location of ScSDO was identifed by TargetP 1.1 prediction and Western Blot analysis. Results The full-length cDNA of ScSDO was 2914 bp, encoding a protein of 304 amino acids. The deduced ScSDO protein was highly conserved, containing the signature HXHXDH motif of the metallo-β-lactamase superfamily and two metalbinding sites, of which metal-binding site I is known to be the catalytically active center. Subcellular localization confrmed that ScSDO was located only in the mitochondria. Responding to the sulfde exposure, distinct time-dependent increases in ScSDO expression were detected at both mRNA and protein levels. Moreover, the gills exhibited a higher ScSDO expression level than the livers. Conclusions All of our results suggest that ScSDO plays an important role in mitochondrial sulfde oxidation during sulfde stress, making S. constricta highly sulfde tolerant. In addition, as a respiratory tissue, the gills play a more critical role in sulfde detoxifcation.
Xue Bai,Yinghui Zhao,Zhenhu Song,Hui Chen,Sihang Zhang,Yonghui Luo,YingChun Gu,Shijian Tu,Guo Yao,Sheng Chen 한국섬유공학회 2022 Fibers and polymers Vol.23 No.3
The preparation of polymer nanocomposites combined with high strength, toughness, and high transparencyremains a challenge. Aramid fibers are often used as fiber-reinforced materials for their superior mechanical and thermalproperties, but the weak interfacial force between aramid fibers and matrix polymer limits the application in compositematerials. In this work, aramid nanofibers were prepared by a two-step process, which included deprotonation and acidhydrothermal treatment to obtain better dispersions in general solvents. The hydrothermal aramid nanofibers (HANFs) wereused as reinforcing materials and blended with polyacrylonitrile (PAN) to prepare polyacrylonitrile/aramid nanofibers (PAN/HANFs) composite films with different mass fractions of HANFs. The morphologies of HANFs and the thermal, optical, andmechanical properties of composite films were investigated. Interestingly, when the mass fraction of aramid nanofibers wasless than 1.0 %, the composite films were synchronously strengthened and toughened. When the mass fraction of HANFswas 0.5 %, the tensile strength and toughness of the PAN/HANFs composite film reached 62.04 MPa and 22.56 MJ/m3,which were 74.23 % and 162.31 % higher than the pure PAN film, respectively. Besides, its average transmittance in thevisible light region remained 76.34 %. This work may offer a novel and facile strategy for high transparent reinforcedpolymer composites, which have potential applications in high strength fiber or optical film.
강장묵,Haibo HU,Yinghui CHEN,이상원 한국인터넷방송통신학회 2019 Journal of Advanced Smart Convergence Vol.8 No.4
The online retail market continues to grow, especially in China, as e-commerce has developed rapidly in recent years in many countries. Meanwhile, the development and use of new network information technology provides consumers with various contact and experience environments for online shopping. Based on the theory of media weakness, the study began to focus consumer experience on the nature of commercial transactions. The study proposed and designed an initial measure of the consumer information evaluation index, which combines previous findings with implications. Finally, the five-dimensional B2C system was established to evaluate consumers' information experience providing information display, information interaction, information support and information personalization. We researched on evaluation index system for information experience of B2C e-commerce consumers based on samples of Chinese consumers.