Colonic Transit Disorder Mediated by Downregulation of Interstitial Cells of Cajal/Anoctamin-1 in Dextran Sodium Sulfate-induced Colitis Mice

Chen Lu,Hongli Lu,Xu Huang,Shaohua Liu,Jingyu Zang,Yujia Li,Jie Chen,Wenxie Xu 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.2

Background/Aims Interstitial cells of Cajal (ICC) and their special calcium-activated chloride channel, anoctamin-1 (ANO1) play pivotal roles in regulating colonic transit. This study is designed to investigate the role of ICC and the ANO1 channel in colonic transit disorder in dextran sodium sulfate (DSS)-treated colitis mice. Methods Colonic transit experiment, colonic migrating motor complexes (CMMCs), smooth muscle spontaneous contractile experiments, intracellular electrical recordings, western blotting analysis, and quantitative polymerase chain reaction were applied in this study. Results The mRNA and protein expressions of c-KIT and ANO1 channels were significantly decreased in the colons of DSS-colitis mice. The colonic artificial fecal-pellet transit experiment in vitro was significantly delayed in DSS-colitis mice. The CMMCs and smooth muscle spontaneous contractions were significantly decreased by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), an ANO1 channel blocker, and NG-Nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of nitric oxide synthase activity, in DSS-colitis mice compared with that of control mice. Intracellular electrical recordings showed that the amplitude of NPPB-induced hyperpolarization was more positive in DSS-colitis mice. The electric field stimulation-elicited nitric-dependent slow inhibitory junctional potentials were also more positive in DSS-colitis mice than those of control mice. Conclusion The results suggest that colonic transit disorder is mediated via downregulation of the nitric oxide/ICC/ANO1 signalling pathway in DSS-colitis mice.

Direct Chemical Vapor Deposition-Derived Graphene Glasses Targeting Wide Ranged Applications

Sun, Jingyu,Chen, Yubin,Priydarshi, Manish Kr.,Chen, Zhang,Bachmatiuk, Alicja,Zou, Zhiyu,Chen, Zhaolong,Song, Xiuju,Gao, Yanfeng,Rü,mmeli, Mark H.,Zhang, Yanfeng,Liu, Zhongfan American Chemical Society 2015 NANO LETTERS Vol.15 No.9

<P>Direct growth of graphene on traditional glasses is of great importance for various daily life applications. We report herein the catalyst-free atmospheric-pressure chemical vapor deposition approach to directly synthesizing large-area, uniform graphene films on solid glasses. The optical transparency and sheet resistance of such kinds of graphene glasses can be readily adjusted together with the experimentally tunable layer thickness of graphene. More significantly, these graphene glasses find a broad range of real applications by enabling the low-cost construction of heating devices, transparent electrodes, photocatalytic plates, and smart windows. With a practical scalability, the present work will stimulate various applications of transparent, electrically and thermally conductive graphene glasses in real-life scenarios.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2015/nalefd.2015.15.issue-9/acs.nanolett.5b01936/production/images/medium/nl-2015-019367_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl5b01936'>ACS Electronic Supporting Info</A></P>

NLRP3-dependent lipid droplet formation contributes to posthemorrhagic hydrocephalus by increasing the permeability of the blood–cerebrospinal fluid barrier in the choroid plexus

Zhang Zhaoqi,Guo Peiwen,Liang Liang,Jila Shiju,Ru Xufang,Zhang Qiang,Chen Jingyu,Chen Zhi,Feng Hua,Chen Yujie 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Hydrocephalus is a severe complication that can result from intracerebral hemorrhage, especially if this hemorrhage extends into the ventricles. Our previous study indicated that the NLRP3 inflammasome mediates cerebrospinal fluid hypersecretion in the choroid plexus epithelium. However, the pathogenesis of posthemorrhagic hydrocephalus remains unclear, and therapeutic strategies for prevention and treatment are lacking. In this study, an Nlrp3−/− rat model of intracerebral hemorrhage with ventricular extension and primary choroid plexus epithelial cell culture were used to investigate the potential effects of NLRP3-dependent lipid droplet formation and its role in the pathogenesis of posthemorrhagic hydrocephalus. The data indicated that NLRP3-mediated dysfunction of the blood–cerebrospinal fluid barrier (B-CSFB) accelerated neurological deficits and hydrocephalus, at least in part, through the formation of lipid droplets in the choroid plexus; these lipid droplets interacted with mitochondria and increased the release of mitochondrial reactive oxygen species that destroyed tight junctions in the choroid plexus after intracerebral hemorrhage with ventricular extension. This study broadens the current understanding of the relationship among NLRP3, lipid droplets and the B-CSFB and provides a new therapeutic target for the treatment of posthemorrhagic hydrocephalus. Strategies to protect the B-CSFB may be effective therapeutic approaches for posthemorrhagic hydrocephalus.

Aerosol Synthesis of Cargo-FilledGraphene Nanosacks

Chen, Yantao,Guo, Fei,Jachak, Ashish,Kim, Sang-Pil,Datta, Dibakar,Liu, Jingyu,Kulaots, Indrek,Vaslet, Charles,Jang, Hee Dong,Huang, Jiaxing,Kane, Agnes,Shenoy, Vivek B.,Hurt, Robert H. American ChemicalSociety 2012 Nano letters Vol.12 No.4

<P>Water microdroplets containing graphene oxide and a secondsoluteare shown to spontaneously segregate into sack-cargo nanostructuresupon drying. Analytical modeling and molecular dynamics suggest thesacks form when slow-diffusing graphene oxide preferentially accumulatesand adsorbs at the receding air–water interface, followed bycapillary collapse. Cargo-filled graphene nanosacks can be nanomanufacturedby a simple, continuous, scalable process and are promising for manyapplications where nanoscale materials should be isolated from theenvironment or biological tissue.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2012/nalefd.2012.12.issue-4/nl2045952/production/images/medium/nl-2011-045952_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl2045952'>ACS Electronic Supporting Info</A></P>

Aerodynamic effects of subgrade-tunnel transition on high-speed railway by wind tunnel tests

Jingyu Zhang,Mingjin Zhang,Yongle Li,Chen Fang 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.28 No.4

The topography and geomorphology are complex and changeable in western China, so the railway transition section is common. To investigate the aerodynamic effect of the subgrade-tunnel transition section, including a cutting-tunnel transition section, an embankment-tunnel transition section and two typical scenarios for rail infrastructures, is selected as research objects. In this paper, models of standard cutting, embankment and CRH2 high-speed train with the scale of 1:20 were established in wind tunnel tests. The wind speed profiles above the railway and the aerodynamic forces of the vehicles at different positions along the railway were measured by using Cobra probe and dynamometric balance respectively. The test results show: The influence range of cutting-tunnel transition section is larger than that of the embankment-tunnel transition section, and the maximum impact height exceeds 320mm (corresponding to 6.4m in full scale). The wind speed profile at the railway junction is greatly affected by the tunnel. Under the condition of the double track, the side force coefficient on the leeward side is negative. For embankment-tunnel transition section, the lift force coefficient of the vehicle is positive which is unsafe for operation when the vehicle is at the railway line junction.

Experimental Study of Adaptive Sliding Mode Control for Vibration of a Flexible Rectangular Plate

Jingyu Yang,Zhiqi Liu,Xuanming Cui,Shiying Qu,Chu Wang,Zhou Lanwei,Guoping Chen 한국항공우주학회 2015 International Journal of Aeronautical and Space Sc Vol.16 No.1

This paper aims to address the intelligent active vibration control problem of a flexible rectangular plate vibration involving parameter variation and external disturbance. An adaptive sliding mode (ASM) MIMO control strategy and smart piezoelectric materials are proposed as a solution, where the controller design can deal with problems of an external disturbance and parametric uncertainty in system. Compared with the current ‘classical’ control design, the proposed ASM MIMO control strategy design has two advantages. First, unlike existing classical control algorithms, where only low intelligence of the vibration control system is achieved, this paper shows that high intelligent of the vibration control system can be realized by the ASM MIMO control strategy and smart piezoelectric materials. Second, the system performance is improved due to two additional terms obtained in the active vibration control system. Detailed design principle and rigorous stability analysis are provided. Finally, experiments and simulations were used to verify the effectiveness of the proposed strategy using a hardware prototype based on NI instruments, a MATLAB/SIMULINK platform, and smart piezoelectric materials.

Early potential effects of resveratrol supplementation on skeletal muscle adaptation involved in exercise-induced weight loss in obese mice

( Jingyu Sun ),( Chen Zhang ),( Minjeong Kim ),( Yajuan Su ),( Lili Qin ),( Jingmei Dong ),( Yunhe Zhou ),( Shuzhe Ding ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.4

Exercise and resveratrol supplementation exhibit anti-obesity functions in the long term but have not been fully investigated yet in terms of their early potential effectiveness. Mice fed with high-fat diet were categorized into control (Cont), exercise (Ex), resveratrol supplementation (Res), and exercise combined with resveratrol supplementation (Ex + Res) groups. In the four-week period of weight loss, exercise combined with resveratrol supplementation exerted no additional effects on body weight loss but significantly improved whole-body glucose and lipid homeostasis. The combined treatment significantly decreased intrahepatic lipid content but did not affect intramyocellular lipid content. Moreover, the treatment significantly increased the contents of mtDNA and cytochrome c, the expression levels of peroxisome proliferator-activated receptor gamma coactivator-1 alpha and its downstream transcription factors, and the activities of ATPase and citrate synthase. However, exercise, resveratrol, and their combination did not promote myofiber specification toward slow-twitch type. The effects of exercise combined with resveratrol supplementation on weight loss could be partly due to enhanced mitochondrial biogenesis and not to fiber-type shift in skeletal muscle tissues. [BMB Reports 2018; 51(4): 200-205]

Theoretical models of threshold stress intensity factor and critical hydride length for delayed hydride cracking considering thermal stresses

Jingyu Zhang,Jiacheng Zhu,Shurong Ding,Liang Chen,Wenjie Li,Hua Pang 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.7

Delayed hydride cracking (DHC) is an important failure mechanism for Zircaloy tubes in the demandingenvironment of nuclear reactors. The threshold stress intensity factor, KIH, and critical hydride length, lC ,are important parameters to evaluate DHC. Theoretical models of them are developed for Zircaloy tubesundergoing non-homogenous temperature loading, with new stress distributions ahead of the crack tipand thermal stresses involved. A new stress distribution in the plastic zone ahead of the crack tip isproposed according to the fracture mechanics theory of second-order estimate of plastic zone size. Thedeveloped models with fewer fitting parameters are validated with the experimental results for KIH andlC. The research results for radial cracking cases indicate that a better agreement for KIH can be achieved;the negative axial thermal stresses can lessen KIH and enlarge the critical hydride length, so its effectshould be considered in the safety evaluation and constraint design for fuel rods; the critical hydridelength lC changes slightly in a certain range of stress intensity factors, which interprets the phenomenonthat the DHC velocity varies slowly in the steady crack growth stage. Besides, the sensitivity analysis ofmodel parameters demonstrates that an increase in yield strength of zircaloy will result in a decrease inthe critical hydride length lC , and KIH will firstly decrease and then have a trend to increase with the yieldstrength of Zircaloy; higher fracture strength of hydrided zircaloy will lead to very high values ofthreshold stress intensity factor and critical hydride length at higher temperatures, which might be themain mechanism of crack arrest for some Zircaloy materials

Research Progress of the Structure Vibration-Attitude Coordinated Control of Spacecraft

Jingyu Yang,Shiying Qu,Jiahui Lin,Zhiqi Liu,Xuanming Cui,Chu Wang,Dujiang Zhang,Mingcheng gu,Zhongrui Sun,Kang Yang,Lanwei Zhou,Guoping Chen 한국항공우주학회 2015 International Journal of Aeronautical and Space Sc Vol.16 No.4

This paper gives an overview of research on the field of structure vibration-attitude coordinated control of spacecraft. First of all, the importance of the technology has been given an introduction, and then later the research progress of space structure dynamics modeling, research progress of structure vibration-attitude coordinated control of flexible spacecraft have been discussed respectively. Finally, future research on application of structure vibration-attitude coordinated control of spacecraft has been recommended.

Dislocation Damping and Defect Friction Damping in Magnesium: Molecular Dynamics Study

Jingyu Zhai,Xinyuan Song,Anyang Xu,Yugang Chen,Qingkai Han 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.6

In this study, the molecular dynamics method was used to study the damping mechanism in Mg alloys at the atomic scale. The energy dissipated by the nucleation and motion of dislocations and by defects friction, and the effect of defects, such asvacancies, cracks, and grain boundaries, on them were studied. The study shows that different kinds of defect have differenteffects on the dislocation damping and defect friction damping. And then, the effect of strain amplitude and temperature ondamping capacity of Mg was studied. The result shows that the amplitude independent damping is caused by defect frictionand the amplitude dependent damping is mainly caused by the nucleation and motion of dislocation; the damping of Mgincreased exponentially with the temperature, and the damping peck appeared at 440 K is attributed to the appearance ofdislocations at the grain boundaries which may be caused by boundaries self-diffusion.