Ghosh와 Kim 모수 δ의 영향함수 유도 및 확인

김민정,김홍기,Kim, Minjeong,Kim, Honggie 한국통계학회 2017 응용통계연구 Vol.30 No.4

Ghosh와 Kim에 의해 소개된 영 변환 모형은 0이 많거나 적을 때 계수형 자료(count data)를 분석하는 모형이다. 이 모형의 산포형태모수는 평균과 분산, 0 확률로 구성되며 ${\mu}$와 ${\sigma}^2$의 관계에 따라 2가지 형태를 가진다. 본 논문에서는 ${\sigma}^2{\geq}{\mu}$일 때, Ghosh와 Kim 영 변환확률 모형의 모수 ${\delta}$에 대한 영향함수를 도출하였다. 도출한 영향함수의 타당성을 검증하기 위해서 인구주택총조사 자료를 이용해 관측치가 제거된 경우에서 영향함수로 도출한 ${\delta}$ 추정치 변화값과 직접 계산한 ${\delta}$ 추정치 변화값을 비교하였다. 그 결과 영향함수는 ${\delta}$의 변화를 매우 정확히 추정하였다. The Ghosh and Kim zero-altered distribution model is used to analyze count data that have too many or too few zeros. The dispersion type parameter ${\delta}$ in the zero-altered distribution model consists of mean, variance and zero probability and has two forms depending on the relation between ${\mu}$ and ${\sigma}^2$. We derived the influence function on ${\delta}$ when ${\sigma}^2{\geq}{\mu}$. To show the validity of the influence function, we used the Census data on the number of births of married women in Korea to compare the estimated changes in ${\delta}$ using this function with those obtained using the direct deletion method. The result proved that the obtained influence function is very accurate in estimating changes in ${\delta}$ when an observation is deleted.

Genetic heterogeneity of liver cancer stem cells

Minjeong Kim(Minjeong Kim),Kwang-Woo Jo(Kwang-Woo Jo),Hyojin Kim(Hyojin Kim),Myoung-Eun Han(Myoung-Eun Han),Sae-Ock Oh(Sae-Ock Oh) 대한해부학회 2023 Anatomy & Cell Biology Vol.56 No.1

Cancer cell heterogeneity is a serious problem in the control of tumor progression because it can cause chemoresistance and metastasis. Heterogeneity can be generated by various mechanisms, including genetic evolution of cancer cells, cancer stem cells (CSCs), and niche heterogeneity. Because the genetic heterogeneity of CSCs has been poorly characterized, the genetic mutation status of CSCs was examined using Exome-Seq and RNA-Seq data of liver cancer. Here we show that different surface markers for liver cancer stem cells (LCSCs) showed a unique propensity for genetic mutations. Cluster of differentiation 133 (CD133)-positive cells showed frequent mutations in the IRF2, BAP1, and ERBB3 genes. However, leucine-rich repeat-containing G protein-coupled receptor 5-positive cells showed frequent mutations in the CTNNB1, RELN, and ROBO1 genes. In addition, some genetic mutations were frequently observed irrespective of the surface markers for LCSCs. BAP1 mutations was frequently observed in CD133-, CD24-, CD13-, CD90-, epithelial cell adhesion molecule-, or keratin 19-positive LCSCs. ASXL2, ERBB3, IRF2, TLX3, CPS1, and NFATC2 mutations were observed in more than three types of LCSCs, suggesting that common mechanisms for the development of these LCSCs. The present study provides genetic heterogeneity depending on the surface markers for LCSCs. The genetic heterogeneity of LCSCs should be considered in the development of LCSC-targeting therapeutics.

A Study of the Reliability and Validity of the Korean Version of the Penn Alcohol Craving Scale for Alcohol-Dependent Patients

MinJeong Kim,SungGon Kim,HyoJeong Kim,HoChan Kim,JiHeh Park,KwangSeok Park,DukKi Lee,WonTan Byun,CheolMin Kim 대한신경정신의학회 2008 PSYCHIATRY INVESTIGATION Vol.5 No.3

Objective-The Penn Alcohol Craving Scale (PACS) is a stronger predictor of subsequent drinking and relapse of alcohol dependence that can be administered more quickly and easily than other craving scales. The goal of this study was to develop the Korean version of the Penn Alcohol Craving Scale (PACS-K). Methods-To examine the psychometric properties of the PACS-K, responses were chosen from 80 patients admitted to a treatment facility for alcohol dependence. Results-The PACS-K possesses good psychometric properties, as assessed by Cronbach’s α estimates (Cronbach’s α=0.91). The test-retest reliability of the PACS-K showed high correlation (p<0.01) when the retest interval was 1 day. When the validity of the PACS-K was investigated using correlation analysis with two other craving scales (the Obsessive Compulsive Drinking Scale (OCDS) and the Visual Analogue Scale (VAS), high correlations were obtained between total PACS scores and total OCDS scores, and between total PACS scores and VAS scores (p<0.01, respectively). Conclusion-The PACS-K is a reliable and valid measure of alcohol cravings, and it could be useful for predicting which individuals are at risk for subsequent relapse.

Development of a Web-Based Education Program for Families Dealing with Schizophrenia

Minjeong Kim,Soyaja Kim,Ilyoung Yoo,Sunah Kim 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

Impact of Glacial Cycles and Sea Level Changes on Radionuclide Transport for Geological Disposal Systems

Minjeong Kim,Heejae Ju,Soobin Kim,Jung-Woo Kim 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.2

Safety assessments for geological disposal systems extend over tens of thousands of years, taking into account the radiotoxicity decay period of spent nuclear fuel. During this extensive period, the biosphere experiences multiple glacial cycles, and fluctuations in seawater amounts, attributed to the formation and melting of glaciers, lead to global sea level changes known as eustacy. These sea level changes can directly influence the land-sea interface and groundwater flow dynamics, consequently affecting the pathways of radionuclide transport - an essential element of dose assessment. Therefore, this study aims to investigate how glacial cycles and sea level changes impact radionuclide transport within geological disposal systems, especially in the biosphere. To achieve this objective, we obtained climate evolution data including sea level changes for the Korean Peninsula over a 200,000-years, simulated by a General Circulation Model (GCM). These data were then employed to predict site and hydrology evolutions. The study site was conceptualized biosphere of Artificial Disposal System (ADioS), and we utilized the Soil and Water Assessment Tool (SWAT) to simulate hydrological evolution. These datasets, encompassing climate, site, and hydrology evolution, were collectively employed as inputs for the biosphere module of Adaptive Process-Based Total System Performance Assessment Framework (APro). Subsequently, the APro’s biosphere module calculated radionuclide transport in groundwater flow and its release into surface water bodies, considering the influences of glacial cycles and sea level changes. The results show that hydrologic changes due to sea level change are relatively minor, while the impact of sea level change on groundwater flow and discharge is significant. Additionally, we identified that among the water bodies within ADioS, including rivers, lakes, and oceans, the ocean exhibits the most substantial radionuclide outflow throughout the entire period. The spatiotemporal distributions of radionuclides computed within APro will be further processed into a grid format and used as input for the dose assessment module. Through this study, it was possible to determine the impact of long-term glacial cycles and sea level changes on radionuclide transport. Additionally, this module can serve as a valuable tool for providing the spatiotemporal variability of radionuclides required for enhanced dose assessments.

A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate

Kim Minjeong,Hur Sumin,김광휘,Cho Yejin,Kim Keunyoung,Kim Ha Ryong,남기택,Lim Kyung-Min 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.2

Liver fibrosis is part of the wound healing process to help the liver recover from the injuries caused by various liver-damaging insults. However, liver fibrosis often progresses to life-threatening cirrhosis and hepatocellular carcinoma. To overcome the limitations of current in vivo liver fibrosis models for studying the pathophysiology of liver fibrosis and establishing effective treatment strategies, we developed a new mouse model of liver fibrosis using polyhexamethylene guanidine phosphate (PHMG-p), a humidifier sterilizer known to induce lung fibrosis in humans. Male C57/BL6 mice were intraperitoneally injected with PHMG-p (0.03% and 0.1%) twice a week for 5 weeks. Subsequently, liver tissues were examined histologically and RNA-sequencing was performed to evaluate the expression of key genes and pathways affected by PHMG-p. PHMG-p injection resulted in body weight loss of ~15% and worsening of physical condition. Necropsy revealed diffuse fibrotic lesions in the liver with no effect on the lungs. Histology, collagen staining, immunohistochemistry for smooth muscle actin and collagen, and polymerase chain reaction analysis of fibrotic genes revealed that PHMG-p induced liver fibrosis in the peri-central, peri-portal, and capsule regions. RNA-sequencing revealed that PHMG-p affected several pathways associated with human liver fibrosis, especially with upregulation of lumican and IRAK3, and downregulation of GSTp1 and GSTp2, which are closely involved in liver fibrosis pathogenesis. Collectively we demonstrated that the PHMG-p-induced liver fibrosis model can be employed to study human liver fibrosis.

PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs

Kim, Yoosik,Park, Joha,Kim, Sujin,Kim, MinA,Kang, Myeong-Gyun,Kwak, Chulhwan,Kang, Minjeong,Kim, Baekgyu,Rhee, Hyun-Woo,Kim, V. Narry Elsevier 2018 Molecular cell Vol.71 No.6

<P><B>Summary</B></P> <P>Protein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find that various noncoding RNAs interact with PKR. Surprisingly, the majority of the PKR-interacting RNA repertoire is occupied by mitochondrial RNAs (mtRNAs). MtRNAs can form intermolecular dsRNAs owing to bidirectional transcription of the mitochondrial genome and regulate PKR and eIF2α phosphorylation to control cell signaling and translation. Moreover, PKR activation by mtRNAs is counteracted by PKR phosphatases, disruption of which causes apoptosis from PKR overactivation even in uninfected cells. Our work unveils dynamic regulation of PKR even without infection and establishes PKR as a sensor for nuclear and mitochondrial signaling cues in regulating cellular metabolism.</P> <P><B>Highlights</B></P> <P> <UL> <LI> fCLIP-seq reveals PKR-interacting endogenously expressed dsRNAs </LI> <LI> PKR binds to various noncoding RNAs such as retrotransposons and satellite RNAs </LI> <LI> MtRNAs can form intermolecular dsRNAs and strongly interact with PKR </LI> <LI> MtRNAs can regulate PKR phosphorylation and signaling, especially under stress </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

A Study of CsI(Tl) Scintillator with Optimized Conditions of Large Area Avalanche Photodiode

Kim, Minjeong,Kang, Heedong,Kim, H.J.,Kim, Wan,Park, H.,Kim, Sunghwan Informa UK (Taylor Francis) 2008 Journal of nuclear science and technology Vol.45 No.suppl5

Improved High-temperature Stability to Solve Cryogenic Transportation and Storage Problems of mRNA Vaccines

Minjeong KIM,Taekyoung LEE,Hyunjin KIM,Heejae BANG,Minji KIM,Minsub CHUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

While the spread of COVID-19 worldwide is currently emerging as a serious social problem, mRNA vaccines with advantages such as rapid development speed and mutation responsiveness are being developed, showing hope for overcoming COVID-19. However, there is a difficulty in transporting and storing mRNA vaccines. We must transport and store mRNA vaccines at cryogenic temperatures because, outside the cryogenic conditions, particle aggregation and leakage occur, which reduces efficacy and causes side effects. Therefore, in order to quickly overcome the COVID-19 pandemic, it is necessary to improve the stability of mRNA vaccines at room temperature. In this study, through the low-temperature decompression concentration method and stabilizer, our goal is maintaining more than 90% of the initial efficacy of the mRNA vaccines at low temperature (2 ~ 8 ℃) for more than 3 months. The low-temperature decompression concentration method and stabilizer can save time required for producing mRNA vaccines and make mRNA vaccines more stable in low temperature (2 ~ 8 ℃). mRNA vaccines in good stability will reduce the difficulties caused by the existing transportation and storage problems and reduce the disposal of the vaccine due to temperature deviation during the breakdown of the freezer, so a great economic and social effects are expected.

The Influence of Life Stress and Sleep Disturbance on White Matter Integrity

Kim Minjeong,Lee Jiye,Kim Nambeom,Hwang Yunjee,Lee Kyung Hwa,Lee Jooyoung,Lee Yu Jin,Kim Seog Ju 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.5

Objective This study investigated whether sleep and stress mutually interact to induce changes in white matter integrity.Methods Diffusion tensor imaging (DTI) was conducted on 36 participants (male=22, female=14; mean age=38.33±12.78 years). Participants were divided into three groups depending on their sleep quality and stress levels: poor sleepers with stress, poor sleepers without stress, and good sleepers. Sleep quality and stress level were evaluated using the Pittsburgh Sleep Quality Index and the Life Experiences Survey, respectively. Fractional anisotropy (FA) values were calculated employing DTI tractography.Results After controlling for age and sex, poor sleepers with stress exhibited a lower FA of the left inferior cerebellar peduncle (ICP) than did poor sleepers without stress (t=2.81, p=0.02). Poor sleepers without stress showed a higher FA of the right middle longitudinal fasciculus (MdLF) than did good sleepers (t=3.35, p=0.006).Conclusion The current study reports the effects of sleep, stress, and their interaction on the white matter integrities of the ICP and MdLF. ICP change seems to be associated with sleep disturbances related to stress, while MdLF change would be associated with sleep disturbances unrelated to stress.