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Usefulness of Skin Explants for Histologic Analysis after Fractional Photothermolysis
( Gyeong Hun Park ),( Sung Eun Chang ),( Seunghyun Bang ),( Kwang Hee Won ),( Chong Hyun Won ),( Mi Woo Lee ),( Jee Ho Choi ),( Kee Chan Moon ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3
Background: Fractional laser resurfacing treatment has been extensively investigated and is widely used. However, the mechanism underlying its effects is poorly understood because of the ethical and cosmetic problems of obtaining skin biopsies required to study the changes after laser treatment. Objective: To evaluate the usefulness of human skin explants for the investigation of fractional photothermolysis. Methods: Full-thickness discarded skin was treated in 4 ways: no treatment (control), fractional carbon dioxide laser, fractional Er:YAG laser, and fractional 1,550-nm erbium- doped fiber laser. Both treated and non-treated skin samples were cultured ex vivo at the air-medium interface for 7 days. Frozen tissue was sectioned and stained with hematoxylin & eosin for histologic examination and nitro blue tetrazolium chloride for viability testing. Results: Skin explants cultured for up to 3 days exhibited histologic changes similar to those observed in in vivo studies, including microscopic treatment zones surrounded by a thermal coagulation zone, re-epithelialization, and formation of microscopic epidermal necrotic debris. However, the explant structure lost its original form within 7 days of culture. The viability of skin explants was maintained for 3 days of culture but was also lost within 7 days. Conclusion: The skin explant model may be a useful tool for investigating the immediate or early changes following fractional photothermolysis, but further improvements are required to evaluate the long-term and dermal changes. (Ann Dermatol 27(3) 283∼290, 2015)
양지혜 ( Ji Hye Yang ),원종현 ( Chong Hyun Won ),장성은 ( Sung Eun Chang ),이미우 ( Mi Woo Lee ),최지호 ( Jee Ho Choi ),문기찬 ( Kee Chan Moon ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.5
Background: Lupus erythematosus tumidus (LET) is a rare subset of chronic cutaneous lupus erythematosus, and LET is clinically characterized by erythematous, succulent, urticarial plaques on the sun-exposed areas and LET is histopathologically characterized by the presence of perivascular and periadnexal lymphocytic infiltrates with interstitial mucin deposition. Objective: We investigated the clinical and histopathological features of LET in Korean patients. Methods: A retrospective study was conducted on 18 patients who were diagnosed with LET between 1993 and 2010. The clinical characteristics, histopathological and laboratory findings, association with other subsets of lupus and the course of disease were analyzed. Results: LET occurred predominantly in females, with the gender ratio being 1:8. The mean age at diagnosis was 31.3 years. Two of 18 patients (11.1%) were previously diagnosed with systemic lupus erythematosus (SLE) or they had discoid lupus erythematosus (DLE) simultaneously. The most common clinical features were erythematous patches, nodules or plaques and the most common site was the face. Eight of eighteen patients (44.4%) had photosensitivity and this value was lower than that of the previous reports. The antinuclear antibody (ANA) was negative or weakly positive in 13/15 (86.7%) cases. The main histopathological characteristics were perivascular and/or periadnexal lymphocytic infiltrates, and interstitial mucin deposition. Epidermal changes were absent or there were minimal changes. The treatments included antimalarial drugs, dapsone and topical and systemic corticosteroid. The patients showed a good response to the treatments without scarring. Conclusion: A clinicopathological review of 18 LET cases revealed results that were similar to those of the previous reports, although there were some differences in the gender ratio and frequency of photosensitivity. Further studies are needed to elucidate the cause of such differences. (Korean J Dermatol 2010;48(5):402~408)
Gray Mold of Day Lily (Hemerocallis fulva L.) Caused by Botrytis elliptica in Korea
Chang, Seog-Won,Kim, Sung-Kee,Hwang, Byung-Kook The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.5
In March 2000, gray mold was found on day lily (Hemerocallis fulva L.) in Korea. Among the symptoms observed was blight or early rot with chlorotic halo of the leaves. All the isolates obtained from the lesions of the diseased plant parts were identified as Botrytis elliptica, based on the morphological characteristics of conidia. Conidia that formed on conidiogenous cells were not in chains, hyaline to pale brown, unicellular, ellipsoidal to obovate with a single hilum at the base, entirely verruculose, and 21-31 x 12-$23\mu\textrm{m}$ in size. Pathogenicity of the fungus was established by artificial inoculation on day lily plants. This is the first record of gray mold on day lily caused by B. elliptica in Korea.
First Report of Sclerotinia Rot Caused by Sclerotinia sclerotiorum on Some Vegetable Crops in Korea
Chang, Seog-Won,Kim, Sung-Kee The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.2
Sclerotinia rot occurred severely on some vegetable crops grown in Namyangju, Yangpyung, and Yangiu areas in Korea in 2001-2002. The crops infected with Scterotinia sp. were Adenophora remotiflora, Armoracia lapathfolia, Angelica acutiloba, Angelica archangelica, Anthriscus sylvestris, Aster tataricus, Beta vulgaris var. cicla, Brassica campestris var. marinosa, Brassica juncea var. laciniata, Chicholium intybus, Lactuca indica var. dracoglossa, Lactuca sativa var. oak-leaf, Petroselinum crispum, and Phyteuma japonicum. The fungus associated with the disease was identified as Sclerotinia sclerotiorum, based on the morphological characteristics of the pathogen. The symptoms were water-soaked spots that enlarged later and became a watery soft rot. Infected parts became yellow and then turned brown, followed by death of the whole plant. White mycelia developed on the upper petioles and leaves and on the soil where these plant parts lay. Then black sclerotia in variable size and shape formed from the mycelial mass. Pathogenicity of the fungus was proven by artificially inoculating each crop. This is the first report of Sclerotinia rot on the listed vegetable crops in Korea.
Gesture based Input Device: An All Inertial Approach
Chang Wook,Bang Won-Chul,Choi Eun-Seok,Yang Jing,Cho Sung-Jung,Cho Joon-Kee,Oh Jong-Koo,Kim Dong-Yoon Korean Institute of Intelligent Systems 2005 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.5 No.3
In this paper, we develop a gesture-based input device equipped with accelerometers and gyroscopes. The sensors measure the inertial measurements, i.e., accelerations and angular velocities produced by the movement of the system when a user is inputting gestures on a plane surface or in a 3D space. The gyroscope measurements are integrated to give orientation of the device and consequently used to compensate the accelerations. The compensated accelerations are doubly integrated to yield the position of the device. With this approach, a user's gesture input trajectories can be recovered without any external sensors. Three versions of motion tracking algorithms are provided to cope with wide spectrum of applications. Then, a Bayesian network based recognition system processes the recovered trajectories to identify the gesture class. Experimental results convincingly show the feasibility and effectiveness of the proposed gesture input device. In order to show practical use of the proposed input method, we implemented a prototype system, which is a gesture-based remote controller (Magic Wand).
A taper tension profile maker in a converting machine
Chang Woo Lee,Jang Won Lee,Kee Hyun Shin,Soon Oh Kwon 대한기계학회 2008 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.22 No.1
Winding is an integral operation in almost every web handling process, and a center-wound roll is one of the suitable and general schemes in a winding system. However, improper internal stresses within a center-wound roll can cause damage such as buckling, spoking, cinching, etc. Wound roll quality and performance are known to be related to the distribution of in-roll stresses. It is therefore necessary to analyze the relationship between taper tension in the winding section and internal stress distribution within the center-wound roll to prevent winding failure. But it is hard to compensate for an undesirable winding roll shape such as starring, buckling, and telescoping. This is because the winding section is the final process in a roll to roll system and has no feedback control system to correct winding roll shape directly. A time varying tension profile and accurate control of it in a winding section is one way to shape the fail-safe in-roll stress distribution of a winding roll. In this study, a new taper tension profile making method is aimed for designing high quality wound rolls. A new method to determine the proper taper tension profile was designed by analyzing the winding mechanism which includes the stress model in center-wound rolls, nip induced tension model, relationship between taper tension profile and telescoping, relationship between taper tension type and internal stress distribution. An auto taper tension profile making method was proposed not only to optimize radial stress distribution but also to minimize lateral error (telescoping). Simulation results show that the proposed method is very useful for determining the desirable taper tension profile during the winding process and preventing defects of winding roll shape such as telescoping, starting, and dishing and so on.