A genetic variation in microRNA target site of <i>KRT81</i> gene is associated with survival in early-stage non-small-cell lung cancer

Lee, S. Y.,Choi, J. E.,Jeon, H. S.,Hong, M. J.,Choi, Y. Y.,Kang, H. G.,Yoo, S. S.,Lee, E. B.,Jeong, J. Y.,Lee, W. K.,Lee, J.,Cha, S. I.,Kim, C. H.,Kim, Y. T.,Jheon, S.,Son, J. W.,Park, J. Y. Oxford University Press 2015 ANNALS OF ONCOLOGY Vol.26 No.6

<P>In this study, <I>KRT81</I> rs3660G>C was associated with survival of patients with NSCLC after surgical resection. Mechanistic study suggested that the G-to-C change caused reduced binding efficiency of miRNA, leading to decreased translational repression, thereby increased <I>KRT81</I> expression. The <I>KRT81</I> rs3660G>C may be a useful prognostic biomarker in early-stage NSCLC patients.</P><P><B>Background</B></P><P>MicroRNAs (miRNAs) have a key role in carcinogenesis through negative regulation of their target genes. Therefore, genetic variations in miRNAs or their target sites may affect miRNA–mRNA interactions, thereby result in altered expression of target genes. This study was conducted to investigate the associations between single-nucleotide polymorphisms (SNP) located in the miRNA target sites (poly-miRTSs) and survival of patients with early-stage non-small-cell lung cancer (NSCLC).</P><P><B>Methods</B></P><P>Using public SNP database and miRNA target sites prediction program, 354 poly-miRTSs were selected for genotyping. Among these, 154 SNPs applicable to Sequenom's MassARRAY platform were investigated in 357 patients. A replication study was carried out on an independent patient population (<I>n</I> = 479). <I>Renilla</I> luciferase assay and reverse transcription-polymerase chain reaction were conducted to examine functional relevance of potentially functional poly-miRTSs.</P><P><B>Results</B></P><P>Of the 154 SNPs analyzed in a discovery set, 14 SNPs were significantly associated with survival outcomes. Among these, <I>KRT81</I> rs3660G>C was found to be associated with survival outcomes in the validation cohort. In the combined analysis, patients with the rs3660 GC + CC genotype had a significantly better overall survival compared with those with GG genotype [adjusted hazard ratio (aHR) for OS, 0.65; 95% confidence interval (CI) 0.50–0.85; <I>P</I> = 0.001]. An increased expression of the reporter gene for the C allele of rs3660 compared with the G allele was observed by luciferase assay. Consistently, the C allele was associated with higher relative expression level of <I>KRT81</I> in tumor tissues.</P><P><B>Conclusion</B></P><P>The rs3660G>C affects KRT81 expression and thus influences survival in early-stage NSCLC. The analysis of the rs3660G>C polymorphism may be useful to identify patients at high risk of a poor disease outcome.</P>

Preparation of binder-free thin film Li₄Ti₅O₁₂ anode with an adjustable thickness through anodic TiO₂ nanotubes

Cha, G.,Lee, H.J.,Choi, J. Elsevier 2013 Current Applied Physics Vol.13 No.8

Binder-free thickness-controllable Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> for application in lithium ion batteries was fabricated by the reaction of Li<SUB>2</SUB>CO<SUB>3</SUB> and anodic nanotubular TiO<SUB>2</SUB> at 800 <SUP>o</SUP>C. As the concentration of Li<SUB>2</SUB>CO<SUB>3</SUB> increased, the thickness of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> film increased, leading to increase in discharge capacity. The Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> film prepared at the optimized concentration of Li<SUB>2</SUB>CO<SUB>3</SUB> of 3.8 x 10<SUP>-6</SUP> mol displayed the maximum capacity of 104 μA h cm<SUP>-2</SUP> at the first cycle, which corresponds to 103 mA h g<SUP>-1</SUP>. We found that excess Li<SUB>2</SUB>CO<SUB>3</SUB> led to creation of LiTiO<SUB>2</SUB> phases in the Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> film, which reduced the discharge capacity. For comparison, a Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> film was prepared by the reaction of Li<SUB>2</SUB>CO<SUB>3</SUB> on a non-anodized Ti foil. In this case, discharge capacity was dramatically reduced due to the formation of Li<SUB>2</SUB>TiO<SUB>3</SUB> phases in Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>, which was confirmed by TEM and XRD analysis.

음란물 접촉과 청소년의 성 가치관의 변화 : 서울 일개 중학교를 중심으로

김가영,김슬기,김지아,박다현,연현숙,오연경,주혜인,차근영 이화여자대학교 간호과학대학 2011 이화간호학회지 Vol.- No.45

This document's purposes are to give basic data in the viewpoint of nursing through investigation on the change of teenager's sexual values resulted from pornography experience. 150 middle school boy students who were in first, second and third grade were surveyed. Investigated place is limited only one school which is located in Seoul city. 123 Data was available among the 150 surveyed data. Collected data are statistically processed using SPSS program. To verify the reliability, Cronbach'a was applied and analysed using t- test, ANOVA. In short, the main results of this survey are as follows. 1. The general characteristics of surveyed students: Most of surveyed students were middle school 3rdgrade(42.5%) who had computer at their room ranked first (47.2%). The average using time of internet was more than 2 hours (35.4%, the highest rate). 76.6% of surveyed students answered that they had experience of watching the pornography. 2. 53.1% of surveyed students, which is the highest ratio, experienced pornography before they entered middle school. The curiosity rank first (57.%) about the question why they watch pornography. In case of students who had no experiences of pornography answered that they didn't watch pornography because they had no concern on pornography (51.6%). 3. On sexual values, the average scores of pornography non-experienced (3.77±0.46, full score: 5..0) are higher than those of pornography-experienced (3.53±0.51), showing statically significant difference against pornography -experienced group. 4. Findings of sexual values scores on general characteristics were as follows; The higher in grades, the lower in scores. Top-graded students gained higher scores of sexual values than low graded students showing statistically significant difference in both case of characteristics.

Leadbetterella byssophila gen. nov., sp. nov., isolated from cotton-waste composts for the cultivation of oyster mushroom

Weon, Hang-Yeon,Kim, Byung-Yong,Kwon, Soon-Wo,Park, In-Cheol,Cha, In-Bae,Tindall, Brian J.,Stackebrandt, Erko,Trü,per, Hans G.,Go, Seung-Joo Microbiology Society 2005 International journal of systematic and evolutiona Vol.55 No.6

<P>A bacterial strain, designated 4M15<SUP>T</SUP>, was isolated from cotton-waste composts used as mushroom cultivation in South Korea. Properties of this isolate were studied on the basis of physiological and biochemical characteristics, fatty acid profile, isoprenoid quinone, DNA G+C content and phylogenetic position based on 16S rRNA gene sequence analysis. The strain was found to form a distinct phylogenetic lineage related to the family ‘<I>Flexibacteraceae</I>’ within the phylum ‘<I>Bacteroidetes</I>’. No recognized species showed >85 % 16S rRNA gene sequence similarity to strain 4M15<SUP>T</SUP>. The fatty acid profile of strain 4M15<SUP>T</SUP> included C16 : 1<I>ω</I>7<I>c</I>/iso-C15 : 0 2-OH (30·5 %), iso-C15 : 0 (24·2 %), iso-C15 : 0 2-OH/C16 : 1<I>ω</I>7<I>c</I> (15·9), iso-C17 : 0 3-OH (10·5 %) and C16 : 0 (5·6 %). The major isoprenoid quinone was menaquinone MK-7. The DNA G+C content was 33·0 mol%. Cells were Gram-negative, strictly aerobic, rod-shaped, non-motile, catalase-positive, oxidase-positive and flexirubin-positive. The strain hydrolysed aesculin, gelatin, starch and tyrosine. Several phenotypic tests could be used to differentiate strain 4M15<SUP>T</SUP> from other members of the family ‘<I>Flexibacteraceae</I>’. On the basis of the data presented, strain 4M15<SUP>T</SUP> should be assigned to the phylum ‘<I>Bacteroidetes</I>’ as a novel genus and species, for which the name <I>Leadbetterella byssophila</I> gen. nov., sp. nov. is proposed. The type strain is 4M15<SUP>T</SUP> (=KACC 11308<SUP>T</SUP>=DSM 17132<SUP>T</SUP>).</P>

여대생의 우울, 스트레스와 문제도박과의 관계

하주희,차민주,조에스더,조세빈,홍소리,황정민,박지윤,강하영,노규상 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49

Purpose: The purpose of this study was to learn about problem-gambling level, depression and stress among female college students. Method: In this cross-sectional design study, a convenient sample of 325 female college students were recruited between September and October, 2014. Measurement used for this study were the CPGI(Canadian Problem Gambling Index), the CES-D(Center for Epidemiologic Studies Depression Scale), and the Life stress scale for college students. Data were statistically analyzed using descriptive statistics, t-test, ANOVA and chi-square test. Result: About 58.5 percent of the participants had experience of gambling; lottery was the most frequently used gambling among participants. 36 participants(11.1%) were problem gamblers. Relationship of smoking status to problem gambler group was statistically significant. There were statistically significant relationships among problem-gambling level(Non-problem, Low-risk, Moderate-risk, High-risk), depression and stress. The problem gambler group had significantly higher trait stress scores compared to the normal group. Conclusion: Problem gambling was a risk factor for both depression and stress among female college students. The proposals of this study are as follows; first, in depth-research are required with more expanded sampling as sample of this study was conveniently gathered, it is hard to generalize the result. Second, a problem gambling prevention program is recommended for low-risk problem gamblers as they have risk to become problem gambler later. Third, nursing interventions for problem gamblers should be investigated with regard to depression and stress level.

OGLE-2016-BLG-0693LB: Probing the Brown Dwarf Desert with Microlensing

Ryu, Y.-H.,Udalski, A.,Yee, J. C.,Albrow, M. D.,Chung, S.-J.,Gould, A.,Han, C.,Hwang, K.-H.,Jung, Y. K.,Shin, I.-G.,Zhu, W.,Cha, S.-M.,Kim, D.-J.,Kim, H.-W.,Kim, S.-L.,Lee, C.-U.,Lee, Y.,Park, B.-G.,P American Astronomical Society 2017 The Astronomical journal Vol.154 No.6

<P>We present an analysis of microlensing event OGLE-2016-BLG-0693, based on the survey-only microlensing observations by the OGLE and KMTNet groups. In order to analyze the light curve, we consider the effects of parallax, orbital motion, and baseline slope, and also refine the result using a Galactic model prior. From the microlensing analysis, we find that the event is a binary composed of a low-mass brown dwarf (49(-18)(+20) Mj) companion and a K- or G-dwarf host, which lies at a distance of 5.0 +/- 0.6 kpc toward the Galactic bulge. The projected separation between the brown dwarf and its host star is less than similar to 5 au, thus it is likely that the brown dwarf companion is located in the brown dwarf desert.</P>

Formate-driven growth coupled with H₂ production

Kim, Yun Jae,Lee, Hyun Sook,Kim, Eun Sook,Bae, Seung Seob,Lim, Jae Kyu,Matsumi, Rie,Lebedinsky, Alexander V.,Sokolova, Tatyana G.,Kozhevnikova, Darya A.,Cha, Sun-Shin,Kim, Sang-Jin,Kwon, Kae Kyoung,Im Nature Publishing Group, a division of Macmillan P 2010 Nature Vol.467 No.7313

Although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and H<SUB>2</SUB> (with a change in Gibbs free energy of ?G째 = +1.3??kJ??mol<SUP>??1</SUP>) has not been considered energetic enough to support growth of microorganisms. Recently, experimental evidence for growth on formate was reported for syntrophic communities of Moorella sp. strain AMP and a hydrogen-consuming Methanothermobacter species and of Desulfovibrio sp. strain G11 and Methanobrevibacter arboriphilus strain AZ. The basis of the sustainable growth of the formate-users is explained by H<SUB>2</SUB> consumption by the methanogens, which lowers the H<SUB>2</SUB> partial pressure, thus making the pathway exergonic. However, it has not been shown that a single strain can grow on formate by catalysing its conversion to bicarbonate and H<SUB>2</SUB>. Here we report that several hyperthermophilic archaea belonging to the Thermococcus genus are capable of formate-oxidizing, H<SUB>2</SUB>-producing growth. The actual ?G values for the formate metabolism are calculated to range between ??8 and ??20??kJ??mol<SUP>??1</SUP> under the physiological conditions where Thermococcus onnurineus strain NA1 are grown. Furthermore, we detected ATP synthesis in the presence of formate as a sole energy source. Gene expression profiling and disruption identified the gene cluster encoding formate hydrogen lyase, cation/proton antiporter and formate transporter, which were responsible for the growth of T. onnurineus NA1 on formate. This work shows formate-driven growth by a single microorganism with protons as the electron acceptor, and reports the biochemical basis of this ability.

소뇌에서 발생한 원발성 악성 임파종 1례

차명호,황성남,김병준,지제근 대한신경외과학회 1981 Journal of Korean neurosurgical society Vol.10 No.2

Primary malignant lymphoma of the central nervous system which is characterized by high radiosensitiveness is rarely reported in the literature. We have experienced a case of recurred cerebellar lymphoma whose criginal site was septal area. Though septa1 tumor disappeared completely after radiation, a large cerebellar tumor was found 2 years thereafter. After decompressive suboccipital craniectomy and partial tumor removal the patient's general condition improved so secondary radiation and chemotherapy were started. In the course of post op therapy, the patient abruptly deteriorated and succumbed.

수무지 첨부손상의 즉시 재건

차규호,우상현,이대훈,설정현 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.3

In the department of plastic surgery, Yeung Nam university hospital, 38 cases of thumb tip injury were immediately reconstructed during last 4 years. We analysed and followed up the patient and obtained following results: 1. Thumb tip injury was most commonly involving in third decade with 18 cases (45%) and occurred predominantly in male with 34 cases (90%). 2. The injuries were mainly caused by press machine with 19 cases (50%). 3. Crushing was predominant nature of the injuries with 19 cases (50%). 4. The thumb tips were most commonly involved at the level of nail matrix with 16 cases (42%). 5. In 28 cases (73%), only thumb tip was injured without other combined injury. 6. Rt. side (23 cases, 60%) was more commonly involved than Lt. 7. Cross-side finger flap revealed the best result for the treatment of distal amputation of nail matrix. Contrarily in proximal amputation, microreplantation had the best result in both the cosmetic and the functional standpoint.

鼻咽腔 脈管纖維腫 : 8例의 臨床 및 病理組織學的 檢索 A Clinico-Pathologic Study on Eight Cases

車日甫,盧寬澤,池堤根,咸毅根 대한이비인후과학회 1967 대한이비인후과학회지 두경부외과학 Vol.10 No.2