sfs유전자의 malE유전자 발현 촉진

정수열,이희영,최용락 東亞大學校附設遺傳工學硏究所 1995 遺傳工學硏究 Vol.- No.2

CRP에 변이가 도입된 crp*¹ 유전자를 통하여 cloning된 sfs(sugar fermentation stimulation)유전자 중 nlp와 sfs4 유전자가 maltose 및 maltodextrin의 운반에 관여하는 유전자들 중에서 MalE 단백질을 증가시킴을 immunoblotling을 통하여 확인했다. 즉 MalE 단백질은 periplasmic에 존재하면서 cytoplasmic membrane에 존재하는 MalFGK 단백질과 반응하여 cytoplasm내로 maltose, maltodextrin을 운반하는 MBP(maltose binding protein)로 밝혀져 있다. Most crp* mutants isolated so far were capable of fermenting lactose despite the absence of cAMP, however, they could ferment other sugar such as maltose in the presence of cAMP. nlp and sfs4 genes, which stimulated the maltose metabolism in a crp*¹, cya::km(MK2001) host system. Especially, MalE protein(malE gene: MBP: maltose binding protein), that is transport protein with maltose and maltodextrin in periplasmic, were increased in the cloned with nlp and sfs4 genes.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

작약의 RAPD 분석을 위한 PCR 최적조건 구명

최인수,김성만,김용철,이충렬,박현철 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

To optimize the PCR condition is one of the most important steps for RAPD analysis. The purpose of this study was to optimize PCR condition in Paeonia. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, and extension) was also conducted. The most appropriate template DNA concentration was 60ng. Clear bands were observed from 2.5mM and 4.5mM of MgCl2 if template DNA concentration and amount of taq polymerase were proper. Amount of taq polymerase for the optimal PCR condition was 0.5unit and 1unit. In the consideration of results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 4 conditions (60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and lunit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperature for the optimal PCR condition was 92℃, 36℃, 72℃.

洛東江 流域의 土壤 特色 및 土地 利用 實態 調査

이충열,김용철,박현철,김성만,최인수 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

1980년에 발표한 자료와 그 후 토양조사를 한 결과를 종합하여 낙동강 유역의 토양을 중심으로 우리 나라의 토양특성을 비롯한 각종 토양해설 자료를 근거로 요약하면 다음과 같다. 우리 나라 지형은 매우 복잡하나 산악이 대부분을 차지하고 있으며, 태백산을 중심으로 동부는 급경사의 지형이 발달하여 있고, 서부는 완만한 경사를 이루는 산악지가 많을 뿐만 아니라 우리 나라의 곡창지대라 할 수 있는 평야지는 한강을 비롯하여 낙동강, 금강, 및 영산강 유역에 분포하고 있다. 산악지의 면적이 많아 농경지의 절반 이상이 경사지에 분포하고 있는 것이 특징이라 할 수 있다. 토양 모재는 산성암 조립질인 화강암 또는 화강편마암이 전국의 2/3 이상 점유하고 있으며 수성암인 혈암 및 사암은 낙동강 유역이 위치한 경상남북도에 많이 분포하고 있다. 석회암은 강원도에 가장 많이 분포하고 있고 경기 및 경북의 북부지방에 약간 분포하며 기타 지역에는 산재하여 분포하고 있다. 이외에도 반암, 안산암, 유문암 및 편암 등이 분포하고 있으며 충적층은 주로 낙동강 유역을 포함한 4대강 및 이들 4대강의 재류에 넓게 분포하고 있다. 정밀 토양조사 결과 면적은 9,577,000ha(논 879,000ha, 밭 1,288,000ha, 과수 및 뽕밭 119,000ha, 초지 103,000ha, 임지 6,425,000ha, 기타 763,000ha), 이 중 낙동강 유역은 논, 밭을 포함한 농경지 240,731ha, 임지 1,132,655ha 및 기타 154,945ha로서 총 면적은 대략 1,565,332ha이다. The summary of the survey, which was based on the published data since 1980, on soil characteristics and land utilization of Korea including the basin of the Nakdong river is as follows : The mountainous area comprises the various physiographic features. The eastern part from Taebaeg Mountain is mostly covered by the steep sloped land and the western part perches on gently sloped hil1y area in which the major productive area is distributed along the alluvial plains of the major river basins, Han river, Nakdong river, Kum river and Youngsan river in Korea. More than half of the cultivated lands occur on the local valleys and mountain foot slopes. Soils from coarse textured acidic crystalline rocks such as granite and granitic gneiss cover more than 2/3 of the country. Soils from sedimentary rocks like shale and sandstone are mainly confined Kyungbuk province, soils from limestone are mainly in Kangwon and partly in Kyungki, Kyungbuk and other provinces, and others are from porphyry, andesite, gabbro, rhyolite and schist. Alluvial soils are mainly developed on four river basins and their tributaries. The area of whole cultivated land covered by the detailed soil survey was turned out to be 9.577.000ha, upland 879.000ha, paddy land 1,288,000ha, orchard and mulberry 119.000ha, grass land 103,000ha, forest 6,425,000ha and the others 763,000ha, In Nakdong river valley, total area was approximately 1,565,332ha and the cultivated area including upland and paddy land was 240,731ha. The area of forest and the others were respectively 1,132,655ha and 154,945ha.

개량조개의 煮熟冷凍品에 關한 細菌學的 硏究

張壽賢,鞠重烈 군산대학교 1978 群山水産專門大學 硏究報告 Vol.12 No.2

VA-N, TMA, Coliforms, E. coli and Bacterial flora in the raw sample of Henclam, in the Henclam by their process and the Henclam by date during the frozen storage were studied. The results were as follows : 1) The value of VB-N and TMA got increased according to the period of frozen storage. But the increased rate of TMA was higher than that of VB-N. 2) The number of General bacteria, Coliforms and E. coli in the sample selected after the third washing were 2.6×106 cells/g, 3.1×102 cells/g and 2.8×10 cells/100g. The sample showd maximum of bacterial contamination compared with others. 3) Pseudomonas and Vibrio of Bacterial flora in the raw Henclam were superior in number. 4) Micrococcus and Moraxella of Bacterial flora in the Period of frozen storage were superior, in number, showing 35% and 25%. 5) Klebsiella aerogenes group of Coliforms showed maximum of 50.7% compared with others in the period of frozen storage.

경동맥 내막 절제술 후 발생한 과관류 증후군 1예

송희정,박광열,정진상,조수진,이순정,허승,김동익,이광호,이병붕 대한신경과학회 1999 대한신경과학회지 Vol.17 No.2

glpD and glpE 유전자의 조절영역 결손변이주기가 전사조절에 미치는 영향

정희태,정수열,최용락 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

glpD와 glpE는 같은 조절 영역 하에서 121 bp 간격을 두고 역방향으로 전사되어지며, 이들 유전자 상류영역에 존재하는 하나의 CRP binding site가 두 유전자의 전사 조절에 관여함을 이미 여러 연구를 통하여 확인되어졌다. 본 연구에서는 glpD와 glpE의 조절영역이 다양한 크기로 결손된 변이주를 작성하였다. 각 변이된 promoter의 발현조절 영향을 보고자 LacZ와의 융합 plasmid를 작성하였고, β-galactosidase 활성을 측정하여 cAMP에 의한 발현조절을 검토하였다. 발현조절 특성을 확인한 결과 CRP binding site 전까지 결손이 된 변이주는 wild와 비슷한 활성이지만 CRP bin-ding site가 없어진 변이주의 경우는 promoter 활성이 크게 감소했고 cAMP에 의한 발현조절은 나타내지 않았다. 한편 CRP의 공통배열을 삽입시키면 회복이 가능하였다. glpE의 조절영역도 glpD와 마찬가지로 다양하게 결손시킨 변이주를 작성하여 promoter 조절활성을 확인한 결과 CRP binding site가 없어진 변이주의 경우는 promoter 활성이 크게 감소하였으며, 결손된 CRP 결합영역을 삽입시키니 cAMP에 의한 전사촉진이 거의 회복되어졌다. The glpD gene encoding gly-3-p dehydrogenase is essential for the aerobic growth of E. coli on glycerol or gly-3-p. The glpE gene, the function of which is unknown, is transcribed divergently with respect to glpD gene. Expression of the adjacent but divergently transcribed glpD and glpE genes is positively regulated by the cAMP-CRP complex. In this study, for a precise investigation of the functional elements in the regulatory region for transcription activation by cAMP-CRP, deletion mutation have been introducted into the regulatory region. The effect of the deletion mutant on transcriptional regulation was tested in vivo by β-galctosidase activity. Deletion mutants in the regulatory region of glpD demonstrated that the presence of the CRP-binding site resulted in an sixfold increase in promoter activity. And also deletion mutants of glpE gene demonstrated that the presence of the CRP-binding site resulted in an eightfold increase in promoter activity. Insertion of 22 bp oligomer in the deletion mutants has shown that the CRP binding site is need for maximal expression of glpD and glpE genes.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 東亞大學校附設遺傳工學硏究所 1994 遺傳工學硏究 Vol.- No.1

대장균의 nlp(Ner like protein) 유전자를 클로닝하여 구조해석을 한 결과 전사조절 단백질인 Ner와는 61%의 높은 상동성을 가지고 있음을 이미 보고한 바 있다. 본 연구는 당 대사 관련 유전자의 발현 조절을 보고자 nlp 유전자를 도입시켜 tac promoter에 의해 대량 발현시킴으로서 lactose대사 관련 유전자 lacZ와 maltose 대사 관련 유전자인 malQ, malP의 유전자 발현이 2.5배에서 최고 8.3배 정도까지 증가됨을 확인하였다. 이는 nlp유전자가 cAMP비존재하에 crp*1와 상호작용하여 maltose및 lactose대사를 촉진시킴을 시사한다. nlp-lacZ 융합 유전자 산물을 Immunoblotting하여 분석한 결과 nlp의 promoter가 in vivo 상태에서 발현되어짐을 확인하였다. tac promoter와 IPTG에 의하여 nlp유전자 산물을 대량 발현시킨 결과 약 10,000Da의 산물을 SDS-poly-acrylamide gel 전기영동으로서 확인하였으며, 부분 정제된 Nlp단백질을 조절 영역의 DNA단편에 결합함으로서 전사조절에 관여하는 것으로 사료되어졌다. An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here we show that expression of the sugar metabolism related genes, lacZ, malQ and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose-and lactose-metabolism coordinately with crp*1 in the absence of CAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified NIp protein bound a specific DNA of the regulatory region of the nlp gene.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here was show that expression of the sugar metabolism related genes, lacZ, malQ, and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose- and lactose-metabolism coordinately with crp*1 in the absence of cAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified Nlp protein bound a specific DNA of the regulatory region of the nlp gene.

Cellulose 생합성 세균의 분리 및 특성

이승진,유주순,정수열,최용락 東亞大學校附設遺傳工學硏究所 1998 遺傳工學硏究 Vol.- No.5

발효시켜서 만드는 감식초에서 시료를 채취하여 배양하고, pellicle을 형성하는 single colony를 cellulose생합성균으로 분리하였다. 분리된 균주는 형태적 특성, 알콜의 재산화 등의 생리, 생화학적 특성 등에 의하여 Acetobacter속으로 분류되었으며, Acetobacter CBI-2라고 명명하였다. Acetobacter CBI-2는 정지배양 시에 기존의 생합성 균주로 알려진 Acetobacter xylinum과 대등한 cellulose생합성 능력을 나타내었다. Acetobacter CBI-2이 생성한 고분자물질의 분해산물을 TLC법으로 확인한 결과 기존 cellulose의 것과 일치하였다. Acetobacter CBI-2에서 genomic DNA를 분리 정제하여 cel A 영역을 probe로 하여 hybridization시킨 결과, 상동성을 나타내어 분리 균주에는 cellulose 생합성 관련 유전자가 존재함을 나타내었다.(1997년 1월 7일 접수, 1997년 2월 24일 수리). A screening was performed to isolate the cellulose-producing microorganisms from vinegar in Korea. The isolated strain was identified as Acetobacter sp. with respect to physiological and biochemical characteristics and designated as Acetobacter CBI-2. Cellulose production of Acetobacter CBI-2 was equal with the well known cellulose-producing bacteria, A.xylinum. The result of separation on thin layer chromatography (TLC) was consistent with the degradation product of native cellulose. The presence of genes required for the cellulose biosynthesis in Acetobacter CBI-2 was confirmed by Southern hybrization.