공연예술축제의 기능과 성격에 따른 조직체계의 효과적인 방향 연구

안병주 경희대학교 체육대학 한국체육과학연구소 2002 體育學論文集 Vol.30 No.-

In this study we study organization and the operation of human resource of a festival of art of performance in the area of art of dance through going over the functions and traits of a festival of art of performance. In the inner part we improve quality of itself. In the outer part we suggest the effective direction of development that a festival of art of performance should take about the system of organization and it also should be able to have the effect of society and culture, national competition, economy and the international exchange of culture.

N-cadherin이 배양 계배 근아세포에서 발현되는 유전자에 미치는 영향

안병태,김영주 가톨릭대학교 자연과학연구소 2003 자연과학논문집 Vol.24 No.-

근아세포는 여러 단계의 근육형성 프로그램을 거쳐 다핵의 근관세포를 형성하며, 근특이 단백질의 합성 및 축적이 일어난다. 근특이 단백질이 합성은 유전자 조절 단백질인 Myo D, myogenin, Myf-5 그리고 MRF-4 등에 의해 조절되며, 이들 단백질이 동형접합체나 이형접합체를 형성함에 따라 DNA와 결합하여, 전사를 조절한다. 근아세포가 응집을 하기 위해서는 cell adhesion molecule이 필요하며, 이 과정에서 칼슘 의존적인 cell-cell adhesion molecule로서 생쥐와 닭의 뇌세포에서 발현되는 N-cadherin이 관여한다. N-cadherin은 골격근 세포나 그 밖의 많은 세포에서도 태아시기에 일시적으로 발현되며, 분화초기에 근아세포에서 발현되어 근세포간의 집합등에 관여한다. 본 연구는 N-cadherin이 근육에서 일시적으로 발현되는 것에 초점을 맞추고, anti-N-cadherin antibody(NCAD2)를 사용하여 N-cadherin을 억제함으로써 발생하는 근육분화의 형태적, 생화학적 변화를 분석하였다. 근아세포 성장에 NCAD2를 처리함에 따라 형태적 분화양상의 차이를 관찰하였고, 융합의 정도가 감소되어 나타났다. 이러한 분화에 작용하는 기작을 보기 위하여 7.5k의 cDNA chip을 사용하여 실험을 수행하였다. 이 실험을 통하여 NCAD2를 처리하였을 때 근세포 분화동안 발현되는 여러 유전자의 발현이 변화하는 것을 관찰하였다. 근세포분화과정에 관여하는 것으로 알려져 있는 Wnt 경로, TGF-beta 경로, ubiquitin경로가 작용하는 것으로 추정할 수 있었다. 이 중에서 근분화에 관여하는 유전자만을 선별하였고, 또한 calponin, integrin, ADAM15, ARNT-like 1 등이 세포-세포, 세포-기질간의 상호 관계에 관련된 유전자의 발현이 증폭됨을 알 수 있었다. 또한 NCAD2를 처리했을 때, 근세포 분화동안 발현되는 MyoD이 발현이 단백질 수준에서 억제됨을 관찰하였다. 이러한 결과들을 종합하여 세포 상호작용을 중재하는 N-cadherin이 근세포 분화에 요구되는 여러가지의 경로에 중요한 역할을 하는 것을 알 수 있었다.

배추(Brassica campestris L. var. pekinensis Makino)의 cDNA library 구축 및 상동성 비교

안주미,권오병,전봉균,이풍연,이정민,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Expressed sequence tag(EST) has a good value to discover a new gene or to study its structure. Some 20 ESTs were generated to obtain new genetic resources of chinese cabbage(Brassian campestris L. var. pekinensis Makino). Poly A+ RNAs were isolated from 10-day-old seedlings grown at 25℃ under the day light. cDNA gene bank was constructed using λ ZAP /cDNA synthesis /Giga Pack Gold Packing kit. About a million clones were able to obtain from the library. All the clones examined so far had insert DNAs. Nucleotide compositions of randomly selected clones were determined by the Sanger mthod. The DNA sequences were compared with those deposited in the GenePept and GenBank database to figure out nucleotide homologies. Two ESTs showed significant similarities to the enlisted sequences. They are chloroplast GADPH subunit of Arabidopsis thaliana and carbonic anhydrase of A. thaliana. The full DNA sequences of the two clones are being determined. The cDNA gene bank constructed. in this experiment will being used to isolate more genes induced by the light in the plant.

국립ㆍ국회도서관에 소장된 무용학 학위논문 현황 및 연구동향 분석

안병주 경희대학교 체육과학연구소 1999 體育學論文集 Vol.27 No.-

The constant efforts to overcome a permanent possibility in the aspect of preservation have continued to be tried out through new methods with present scientific civilization. The efforts and attempts for these new methods stimulate the need for a theoretical specialized trait of dancing and the various supports of learning research in dancing field. The study of dancing can be acknowledged as a settled learning not abiding by a series of formal process if the task scope of dancing is definitely arranged to establish peculiar methodology systematically. The diverse groping based on precedent researches must be unpartially preceded to present future-oritinted direction of dancing study. Therefore, centering around master and doctor's theses owned in the National Assembly Library from 1960 to 1999, the analysis on the range and distribution of researches is required.

고층빌딩공사의 마감자재 양중계획 수립과정에 대한 실태조사 연구

안병주 전주대학교 공학기술종합연구소 2002 전주대학교 공학기술종합연구소 학술논문집 Vol.8 No.1

This article is a basic study for making a basis on the reasonable lift planning process for finish materials in high-rise building construction. The objective of this study is to analyze the cases of the lift planning process and to address a questionnaire to planners on the actual condition of the process. The results are the lack of information and the low-utilization of lift facilities. Author can understand the actual condition of the lift planning process and realize that planners need a reasonable lift planning process, which can investigate the application possibility of the planning at site.

문자 Media의 기능으로서의 무용 비평

안병주,김효순 경희대학교 체육과학연구소 1997 體育學論文集 Vol.25 No.-

"Dance criticism" is one of the major branches of art criticism. There are various filds of criticism such as 'literary criticism', 'music criticism', 'social criticism', 'journal criticism', 'economical criticism'. even 'interior criticism' which deals with such a minute area of our daily living like interior design. In the criticism of art, there are supposed to be a branch called 'dance criticism'. 'Dance criticism' is a separate entity as an academic field, but it doesn't mean that dance criticism has its own discipline or principles which cannot be applied to other branches of criticism, In other words, dance criticism is not different in its nature from other criticism of art. Of course, it should be admitted that every field of criticism has its own techniques of analysis and standards of evaluation. but principles applied to dance should be relevant in any other fields of art criticism. Charles Augustin do Sainte-Beuve(1804-1869), a French literary Critic in the 19th Century, defines the condition of criticism as "not having one's own art or style". According to him, if a critic has his own style. he would like to fit the work of art in question into his particular thought or style, which means that his critique can be biased with his own preconceptions and Interests. So we should say that critics are supposed to do justice to a given work of art without any prejudice. Now we define the task and function of the dance criticism as follows : First, reasonable and disinterested judgment can be achieved only by horizontal or synchronic comparison and contrast with other fields of art. Second. dance critic should have extensive knowledge of the history and the theory of art, which enables him to carry out diachronic research. Third. a critic should know what is possible and what is impossible in the actual creation of the work. Dance critics should be aware of these principles, if he is to be a great critic. The tack of dance criticism in our time is to make the performers and the audience understand each ocher better, to familiarize them to direct them to appreciate the work of art more properly and, finally, to show the way of progress in the liven field of art.

경직성 뇌성마비아동의 도형-배경 색상역전과 시지각 및 시각-운동 수행능력

최은주,안병즙 大邱大學校 再活科學硏究所 1993 再活科學硏究 Vol.11 No.1

The present study was designed to investigate the effect of color reversal of figure-ground on visual perceptional and visuo-motor performance of cerebral palsied. The 20 spastic cerebral palsied and the 20 non-cerebral palsied children participated in the study as subjects. Perceptual subtest of perceptuo-Motor Development Diagnostic Test(PMDT) and Beery-Buktenica Development Test of Visual-Motor Intergration(VMI) were administered to assess visual perceptual and visuo-motor performance, respectively. The main conclusions from the study were as follows. First, the cerebral palsied subjects performed more poorly in both visual perceptional and in visuo-motor tasks than non-cerebral palsied counterparts. However, it was revealed that the relatively poor performance of the former in visual perceptual tasks were primarily due to Synthesis of Figure factor. Second, for spastic cerebral palsied subjects, the color reversal of testing materials had the facilitating effect on visual perception but not on visuo-motor. However, any improvement as a function of color reversal was evidenced neither in visual perceptioanl nor in visuo-motor performance of the non-cerebral palsied. Finally, presentation order did not produce significant improvement of visual perceptional and visuo-motor performance in either group. It suggest thath improvement by color reversal did not result from presentation order.

신경과 근세포의 응집에 미치는 127 Kda과 135 Kda단백질의 영향

김영주,안병태 가톨릭대학교 자연과학연구소 1996 자연과학논문집 Vol.17 No.-

세포가 접합을 형성할 때 여러 종류의 단백질이 관여하는데, 이는 칼슘 의존계, 칼슘 비의존계, 그밖의 단백질 등, 크게 세가지 계의 단백질로 나눌 수 있다. 칼슘 의존계의 단백질은 칼슘에 의해서 분자 구조가 바뀌는 특성이 있으며, 트립신 등의 단백질 분해효소에 의해 접합 물질들이 변성된다(Takeichi, 1977). 이러한 칼슘 의존계 접합물질의 존재여부를 알아보기 위해 CaCl₂, EDTA, 트립신을 차등을 두어 처리하고 응집율 변화를 관찰하였다. 그 결과, E, TC, TE, LTE 처리구에서 응집율의 차이를 볼 수 있었으며, 신경아세포와 골격근아세포의 접합과 응집에 관여하는 물질 중 칼슘 의존계와 비의존계가 있음을 알 수 있었다. 한편, 신경아세포와 골격근아세포를 같이 배양하여 이들 세포가 서로 응집하는 것을 알아보기 위해, FITC로 생체염색을 하였다. 그 결과 E 처리구와 TC 처리구에서 세포간의 응집이 일어난 것이 관찰되었다. TE 처리구에서는 응집이 일어나지 않았다. LTE 처리구에서도 간혹 응집이 일어난 것이 관찰되었으나 대부분 응집이 일어나지 않았다. 이로서, 칼슘 의존계의 단백질이 신경아세포와 골격근아세포의 상호 응집에 관여한다고 추정할 수 있었다. 응집에 관여하는 단백질의 변화를 보기 위해 전기영동을 한 결과, E 처리구와 TC 처리구의 시료에서 135Kda, 127Kda의 두 단백질을 관찰할 수 있었다. TE 처리구와 LTE 처리구 등 칼슘 의존계가 소멸된 시료에서는 관찰되지 않았다. 따라서, 칼슘 의존계에만 관여하는 단백질의 존재가 확인되었다. 이는 N-cadherin으로 추정되어지나, immunoblot을 통한 검정과정이 요구된다. 이러한 결과를 종합하여 보면, 신경아세포와 골격근아세포에 동일한 칼슘 의존계 단백질이 존재하며, 이 단백질이 신경아세포와 골격근아세포의 접합에 영향을 미친다는 것을 알 수 있었다. 그러나, 신경아세포와 골격근아세포의 접합에 대한 보다 세밀한 생리학적 연구와 신경아세포와 골격근아세포의 연접부위에서의 단백질의 분포등 생화학적 연구가 뒷받침되어야 하며, 135Kda과 127Kda 단백질에 대한 면역학적 분석이 요구된다. There are several kinds of cell adhesion proteins which can be divided into three major groups. Those are the calcium-dependent system, the calcium-independent system, and a third system unrelated with calcium. Calcium-dependent system has several characteristics which reveal calcium-sensitive conformational changes, trypsin and protease-sensitive degradation. In this study, CaCl₂, EDTA, and trypsin were treated to conform the existence of these adhesion molecules. As the results, there were aggregation rate differences among E, TC, TE, and LTE treatments. The results suggest that there are calcium-dependent system and independent system which are related with neuron-muscle adhesion and aggregation. To show muscle cells aggregated with neurons, FITC vital staining was employed. TC and E treated cells were aggregated each other but LTE and TE treated cells were not aggregated. It suggests that calcium-dependent proteins were related to neuron-muscle aggregation. To detect the proteins related to aggregation, electrophoresis was employed. 127 Kda and 135 Kda proteins were detected on E and TC treated samples. However they were not detected on TE and LTE treated samples with eliminated calcium dependent system. Thus, it suggests that calcium-dependent adhesion proteins participated in neuron-muscle aggregation. N-cadherin was thought to be a candidate, but further studies are needed. Taken together, neuron and muscle have same calcium-dependent proteins and these proteins affect neuron-muscle aggregation. Futher immunological as says to identify 135 and 127 Kda proteins are needed and morphological studies about proteins distribution of neuromuscular junction are needed.

Trp P-1 변이원성에 대한 Resveratrol의 항돌연변이 효과

장귀현,안병용,권용주,최동성 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.4

Trp P-1에 의해 유도된 돌연변이원성에 대한 resveratrol의 억제효과를 Salmonella typhimurium TA 98, 100를 이용한 Ames test법으로 검색하였다 먼저 틀변경 돌연변이를 일으키는 S. typhimurium TA98과 염기치환 돌연변이 특성을 지닌 S.typhimurium TA 100에서 Trp P-1의 변이원성을 비교한 결과 S. typhimurium TA98에서 복귀돌연변이의 뚜렷하게 수가 증가되었다. 이러한 결과는 Trp P-1의 돌연변이 유발은 틀변경을 주로 일으킨 돌연변이 유도란 사실을 뒷받침하는 결과로 해석되며,S. typhimurium TA98에서 Trp P-1의 최적농도는 400 ng로 나타났다. S. typhimurium TA98의 생육에 영향을 미치지 않는 농도까지 resveratrol의 농도를 assay당 2, 10, 25, 50 100, 300 ㎍으로 증가시켰을 경우 항돌연변이 효과는 각각 13%, 30%, 52%, 65%, 81% 및 89%로 나타났다. 이러한 결과로부터 Trp P-1의 변이원성에 대하여 resveratrol은 강한 돌연변이 억제효과를 나타내며, 용량 의존적으로 억제함이 확인되었다. The antimutagenic activity of resveratrol on the mutagenicity induced by Trp P-1(3-amino-1,4-dimethyl-5H-pyrido{4,3-b}indole) was studied using the Ames test with Salmonella typhimurium TA98 and 100. Trp P-1 showed strong mutagenecity in S. typhimurium TA98, but was higly decreased mutagenecity in S. typhimurium TA100. This result suggests that the mutagenecity of Trp P-1 can be mainly induced by the DNA lesions causing frame shift. Resveratrol itself did not show antibacterial effect upon 300 rig/assay. Resveratrol showed the strongest inhibitory effect with dose dependent manner on the mutagenicity induced by Trp P-1. The inhibition rates of resveratrol at concentration of 2, 10, 25, 50, 100, 300 ㎍/assay were 13%, 30%, 52%, 65%, 81%, 89%, respectively.

허혈 유사 조건이 심방근의 활동전위 특성에 미치는 영향

김재하,주정민,조용범,신동호,안병희,주신배,김상형 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11