Dog leukocyte antigen identity among cloned dogs with same donor cell

Ji Eun Kim,Hyun Ju Oh,Jung Eun Park,Min Jung Kim,Goo Jang,Byeong Chun Lee 한국발생생물학회 2010 한국발생생물학회 학술발표대회 Vol.29 No.-

The canine major histocompatibility complex (MHC) is referred to dog leukocyte antigens (DLA), which is known to be the most polymorphic genetic system in canine species. Many cloned dogs have been produced since Snuppy, first cloned dog, there was no research about genetic identity of MHC among cloned animals. Recently in Lee’s group, two non-transgenic cloned beagles (BG1, 2) were produced by somatic cell nuclear transfer (SCNT) using fetal fibroblast (BF). Also, four transgenic cloned beagles (Ruppy 1-3, 5) were generated using transgenic BF transfected with Red fluorescent protein (RFP) gene. We hypothesize that non-transgenic (BG1, 2) and transgenic (Ruppy 1-3, 5) cloned beagles derived from identical donor cells have the same immunological genetic characteristic except for RFP gene insertion in the genome. Thus, the aim of this study is to confirm the immunological identity of DLA class II in cloned beagles produced using same nuclear donor cell. Genomic DNA was extracted from blood of BG1, BG2, Ruppy 1, 2, 3 and 5. Genomic DNA of normal two control beagle, no correlation with BF was also investigated for rulling out the possibility that beagles were inbred. Forward and reverse primers used for DLA-DQA1 and DQB1 respectively were DQAF: 5’-TAAGGTTCTTTTCTCCCTCT-3’ and DQAR: 5’-GGACAGATTCAGTGAAGAGA-3’ DQBR:5’-CTCACTGGCCCGGCTGTCTC-3’ and DQBR: 5’-CACCTCGC CGCTGCAACGTG-3’. Polymerase Chain Reaction (PCR) products were purified, sequenced directly using the Big Dye Terminator kit. Sequencing analysis was performed on an automated 3730xl DNA analyzer. In experiment 1, sequence of DLA-DQ alpha 1 (DQA1) and DLA-DQ beta 1 (DQB1) exon 2, hypervariabel region, was compared in BG1 and BG2. Experiment 2 also compared the sequence of DQA1 and DQB1 among Ruppy 1, 2, 3 and 5. Experimental 3 compared sequence of DQA1 and DQB1 among all cloned dogs (BG1, BG2 and Ruppy 1-3, 5). As a result, BG1 and BG2 have same allele for DQA1 and DQB1 as we expected. They share DQA1*00101 and DQB1*02901 in experiment 1. In experiment 2, Ruppy 1, 2, 3 and 5 also have identical DQA1*00101 and DQB1*02901 allele. No discrimination between transgenic dogs and cloned dogs was seen in DQA1 and DQB1 Allele in experiment 3. DQA1, DQB1 allele was identified as *00101 and *02901 in all dogs. We provided the allele identity of DQA1and DQB1 in cloned beagles, which can be used as preliminary data for immunological related studies. In conclusion, transgenic cloned dogs despite of red fluorescent protein genes being inserted in their nuclear DNA were immunologically compatible with non-transgenic cloned dogs. We demonstrated that cloned beagles produced using identical nuclear donor were immunologically compatible.

만성 간질환에 대한 DDB(Biphenyl Dimethyl Dicaboxylate)의 임상적 효과

이헌영,임의혁,김성걸,김진희,육은주,성자원,김병호,정형용,강대영,김영건 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

To investigate the effects of short-term administration of DDB(Biphenyl Dimethyl Dicarboxylate) for decreasing elevated serum aminotransferase levels in chronic liver diseases, authors applied 3 capsules of DDB per day at each meal time for 35 patients and compared with 35 patients treated with other conservative management. The following results were obtained. 1. Serum alanine aminotransferase (ALT) levels significantly decreased from 247.1± 180.0 IU/L of pretreatment level to 96.6±126.0 IU/L, 61.1±62.7 IU/L and 38.0±26.8 IU/L after 1, 3 and 6 months of treatment(p<0.05) and effective rates were observed in 60.0%, 71.4% and 88.6% of patients after 1, 3 and 6 months of treatment. 2. Serum aspartate aminotransferase (AST) levels decreased also significantly from 167.8±96.9 IU/L of pretreatment levels to 124.4±155.0 IU/L, 77.9±49.6 IU/L, 60.6± 46.9 IU/L after 1, 3 and 6 months of treatment(p<0.05), but decreasing pattern of serum AST was less significant than that of serum ALT. 3. No significant difference were observed in the serum ALT and AST changes according to the cause and duration of hepatitis. 4. No significant adverse effects were observed in whole patients treated with DDB. It is suggested that small dose administration of DDB is effective for decreasing serum aminotrans ferase levels in chronic liver disease in which other conservative management was not effective.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

수업용 CD-ROM 제작 및 이를 적용시킨 효과적인 학습지도안 개발 : 중학교 1학년 가정 한복 입기를 중심으로 In the Point of Wearing Korean Traditional Costume for First Grade of Junior Middle School Students in Home Economics Teaching

이은선,김병미 한국 가정과 교육 학회 1999 한국가정과교육학회지 Vol.11 No.2

The goals of the research are for producing and optimizing the CD-ROM effective and practical Teaching-Learning method. It consists of Wearing Korean Tradition Costume for the First Grade of Middle School Students in Home Economics Teaching. This research's summarization in following. First, the multi- media material, CD-ROM making use of Powerpoint. Wearing Korean Traditional Costume is produced to help the students learn the difficult contents in terms of video and audio. Second, It is introduced the model of "Open Education for increasing the efficiency f class. Third, it is developed to proceed the class with the CD-ROM and small group study of place activity. Fourth, it helps students concentrate on the class with proper sound effect whenever the slide films are changed. And it helps to link the web sited related to Korean Traditional Costume. Finally, another kinds of suggestions are following. The effective verification of this software that is tested and applied at the field for a given period willbe necessary. And, it is necessary to upgrade for the CD-ROM and the supplementary teaching materials in Korean Traditional Costume education.

中性시스템에서 白水의 再循還과 脫水 條件이 內添 藥品의 擧動에 미치는 影響

이은정,김병수,정현채,김철환,박종열 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.4

중성 초지에서 공정의 최적화를 위해 사용되는 각종 첨가제로 인해 백수 시스템은 산성 초지보다 훨씬 복잡한 특성을 나타내고 있다. 특히, 환경 및 경제적 이유로 공정수의 완전한 재사용을 위한 노력이 계속되고 있으며, 백수의 재사용은 제지 공정에 있어서 원료의 절감과 폐수의 부하를 줄일 수 있는 잇점이 있기 때문에 지속적인 연구가 이루어지고 있다. 그러나 초지 공정에서 배출되는 백수는 각 위치에 따라 그 특성이 다름에도 불구하고 통합되어 관리되고 있다. 따라서 본 연구에서는 백수의 재순환 회수와 탈수부 압력을 달리 했을 때 탈수부의 위치와 조건에 따른 백수의 유기 성분, 무기 성분과 수질 특성 그리고 이들 조건이 미세분 보류도와 종이의 특성에 미치는 영향을 파악한 결과 다음과 같은 결론을 얻었다. ① 백수의 재순환과 탈수부 조건에 따른 유기 및 무기 성분에 대하여 각각 FTIR과 WRD를 사용한 결과, 제지 공법에 사용되는 각종 물질의 추적을 위해 효과적인 방법으로 판단 되었다. ② 백수의 재순환 회수와 탈수 압력에 따른 수질 분석 결과 자연 탈수부에서는 백수의 재순환 회수가 증가할수록 무기염, 탁도, COD는 증가하였으며 진공 탈수부에서 보다 무기염, 탁도 및 COD는 낮은 것으로 나타났다. ③ 백수의 재순환 회수와 탈수 압력에 따른 종이의 강도적 특성과 미세분 보류도의 결과를 비교한 결과 이들간에 밀접한 관련이 있었다. ④ 백수의 재순환 회수에 따른 자연 탈수부와 진공 탈수부에서 탈수 조건에 따라 백수의 특성이 달라진다는 것을 알 수 있었다. 특히 무기염의 함유량, 탁도, COD 그리고 양이온 요구도에 있어서 자연 탈수부와 진공 탈수부 조건 사이에 나타난 결과에 따라 자연 탈수부에서 수집된 백수는 진공 탈수부에서 수집된 백수보다 미세분, 섬유, 충전제 및 기타 첨가제들을 많이 함유하고 있었으므로 탈수조건에 따라 백수의 이원화 관리가 필요한 것으로 판단된다. There has been shown more complicate characteristics of white water in the alkaline paper making system than that of acid paper making system. Because the alkaline wet end system is consisted with various functional chemicals to control optimum one pass retention on the wire part and chemi-mechanical properties of final paper, especially perfect reuse of white water due to environmental economical view point has been performed. However, in the paper making system, discharged white water in different position and physical conditions to remove in wet web is managed in same line. Therefore, this reserch was performed to identify organic, inorganic material in white water and water recycling and vacuum intensity on the chemical characteristics in the water and properties the paper. The results obtained are summarized as follow: 1. FTIR and XRD which were used for identifying wet end chemical were effective instrument to chase the chemicals from wet end stock to white water.88 2. According to control white water recycle number and vacuum intensity, the characteristics of white water were changed. 3. In condition of atmospheric pressure, apparent amount of fines and several chemicals which produce environmental problom was higher than that of high vaccum condition. 4. Paper mill has to control the white water differently on condition of vacuum and to separate from white water discharged in atmospheric pressure and high vacuum intensity 5. It can be very effective white water management to reduce water pollution and to improve runnability.

運動選手의 意識構造에 關한 硏究(I) : 排球選手를 中心으로

李殷洪,尹炳烈 弘益大學校 1983 弘大論叢 Vol.15 No.2

We can make a conclusion like this through examining and analysing the psychological structure about the sports centering around men's university and business players of all over the country. 1. Conclusion 1) The motivation to become a volley ball player is due to the proposal of parents and acquaintances(including the teachers and seniors.) The principle reason is 45.3% which is saying that the environmental conditions and postulates can find talent in a good player and improve that talent. 2) In the part of the satisfaction's degree in the player's life, "I am satisfied" is 44.2% and "I am not satisfied" is 37.4% and the principle reason of satisfaction is because of a beneficial job and in preserving their own good health and the largest reason of unsatisfaction is that because there is no future and they have a low apititude. I could reckon that it is followed to find the aptitude of players well and improve the player's welfare. 3) In the part of occupation but player which they want to choose, to be teacher approaching 40.5% is the most desirable branch, and the field of administration and commerce to be also expressed 20% says that they want to be convinient economically and study the field to be able to use book in the rest time. 4) This results say that most of them read the book in the rest time. 5) In the satisfaction's degree of only business players. 51.2% of them are not satisfied of their remuneration, and the reason of that result is that they are hard to earn the life and allowance like bonus etc. is too small, and so they aren't satisfied of the remuneration, therefore I could think that the fundamental countermeasure to preserve and rear the players, similarly incomes must be firstly edified. 6) The view that Olympic game must be continues to be enforced in spite of political and ideological polutions is 45.3% and the contrary view is 44.7%, which is almost similar with each other and expressed such phenomenons and the most principle reason to be enforced is expressed that it gives us many chances of cultural exchange and the most principle reason not to be is expressed that between policy and sports must be distinguish and I could think that they have the considerable negative thoughts of political usage and intervention to sports. 7) About the custody of 88th Seoul Olympic, 86.3% of them think it good phenomenon because the possibility of all national development through exchange of culture and economy is more important than that of sports, and negative view is seemed to prevent and object because of economical blow of citizens. 8) In Olympic game, the view that there is the signification in the attendance is 50% because, as I think olympic is the sacred festival and to the contrary, the players who think that there is no siginification in the attendance is 47.9%, for they think that the games without victory don't exist. I could think because the phenomenon that the recenter it comes, the stronger desire of victory reveals controlling trend. 2. Overture 1) If I propose on the basis of research till now, (1) in research's subject, if women player is also examined, it will be more overall "contrast study" and if be distinguished from men and women, university and business to contrast, and determine the quarter of research. I think that it will be seemed to be going to be a better study. 2) I could think that if the solving term of psychological reason is actually inserted and studied on the basis fo this study in guarding the players, it will be an important file.

임상가를 위한 외상후 스트레스 장애 척도의 신뢰도, 타당도 연구

이병용,김임,이선미,은헌정,김동인,김지연 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적 : 본 연구의 목적은 임상가를 위한 외상후 스트레스 장애 척도의 신뢰도와 타당도를 알아보고자 하는 것이다. 방 법 : 28명의 PTSD 군, 30명의 Non-PTSD군, 36명의 정상군에 대하여 임상가를 위한 외상후 스트레스 장애 척도를 실시하였다. 면담자간 신뢰도를 평가하기 위하여 10명의 외상후 스트레스 장애 환자를 2명의 검사자가 동시에 평가하였다. 타당도를 평가하기 위하여 모든 피검자들을 대상으로 사건 충격 척도, Beck의 우울 검사, Spielberger의 상태 특성 불안 검사 Ⅰ, Ⅱ를 시행하였다. 결 과 : Cronbach's α와 면담자간 일치도는 각각 .95와 .89였다. 임상가를 위한 외상후 스트레스 장애 척도는 각각 사건 충격 척도(r=.80), Beck의 우울 검사(r=.70), Spielberger의 상태 특성 불안 검사 Ⅱ(r=.56)와 높은 상관을 가지고 있었다. 그러나 Spielberger의 상태 특성 불안 검사 Ⅰ(r=.20)과는 상관을 보이지 않았다. 임상가를 위한 외상후 스트레스 장애 척도는 임상 진단과 82.1%의 일치도를 보였다. 결 론 : 임상가를 위한 외상후 스트레스 장애 척도는 충분한 신뢰도와 타당도를 가지고 있었다. 임상가를 위한 외상후 스트레스 장애 척도는 외상후 스트레스 장애를 진단하기에 유용한 도구라 할 수 있다. Objectives : The purpose of this study was to examine the reliability and validity of A Clinician-Administered PTSD Scale (CAPS). Methods : CAPS was administered to 28 PTSD subjects, 30 non-PTSD subjects, and 36 normal subjects, Interrater reliability for the CAPS was established by interviewing 10 PTSD subjects. The interviews were conducted by 2 interviewers simultaneously. The authors administered to all the subjects Impact of Event Scale(IES), Beck Depression Inventory(BDI), State Trait Anxiety InventoryⅠ, Ⅱ(STAI-I, Ⅱ) for measuring concurrent validity. Results : The value of Cronbach's α and interrater agreement were .95 and .89, respectively. The CAPS was highly correlated with IES(r=.80), BDI(r=.70), STAI-Ⅱ(r=.56). But the CAPS was not correlated with STAI-I(r=.20). The CAPS showed an overall agreement with clinical diagnosis of 82.1%. Conclusions : The CAPS shows a reasonable degree of reliability and validity. The CAPS could be a valuable tool to diagnose PTSD.

Response of appetite-related genes in relation to the rearing water temperature in red spotted grouper (Epinephelus akaara)

Eun-Jeong Jeon,Byeong-Hoon Kim,Chi-Hoon Lee1,Young-Don Lee 제주대학교 해양과학연구소 2020 해양과환경연구소 연구논문집 Vol.44 No.-

Growth of fish is controlled by various environmental factors, including water temperature (WT). WT is also a major factor that affects the eating behavior of fish. In this study, we studied the relationship between WT and expression profiles of factors involved in appetite regulation and digestion in red spotted grouper. Fish were divided into three groups with 30 fish per group and reared for 2 weeks in a recirculating system at three different WT (natural WT, 20 °C, and 25 °C). After 2 weeks, they were sacrificed immediately after food intake (+ 0 h), 3 h after (+ 3 h), 6 h after (+ 6 h), and 3 h before being fed the next day (− 3 h). Fish samples were obtained for analysis of mRNA expression levels of cholecystokinin (CCK), leptin A (LepA), leptin B (LepB), and neuropeptide Y (NPY) in the brain, liver, and intestine. Expression of CCK and NPY mRNA was significantly higher at 25 °C compared to that at natural WT and 20 °C, but no difference was observed for leptin. This suggests that the red spotted grouper shows characteristics typical of subtropical fish and has higher levels of feeding and digestion when reared at 25 °C water temperature condition.

菜蔬類의 原形質體融合에 관한 硏究

李炳基,殷鍾旋,高正愛,韓光洙 전북대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.1 No.1

This study was carried out to investigate the optimal conditions for producing somatic cell hybrids of vegetables. The conditions investigated are enzyme concentrations and duration of treatment for isolation of protoplast, concentration of PEG 4000 for fusion of isolated protoplasts, and viability of isolated protoplasts and fused protoplasts. The results obtained are as follows: 1. The size of onion protoplasts which are isolated from white mesophyll tissue is 52.6μm. The size of welsh onion and nanking shallot protoplasts is almost uniform (about 45μm) but the size of tomato and potato protoplasts isolated from callus tissue is various(21 to 86μm). 2. The optimal conc. of enzyme for isolation of protoplasts in the mesophyll tissue of radish, chinese cabbage, welsh onionm, and nanking ahallot are 0.5% macerozyme and 1.0% cellulase. While the onion is 0.5% macerozyme and 1.5% cellulase. 3. The optimal duration of enzyme treatment for produced intact protoplasts were 4hr in chinese cabbage, 5hr in radish, 3hr in welsh onion, and 2hr in nanking shallot and onion respectively. 4. Isolated protoplasts from mesophyll tissus were fused easily and produced the hetero-karyon with 25-35% PEG 4000 in inter-or intraspecies, while protoplasts of tomato and potato derived from cell were not fused easily. 5. The isolated protoplasts were viable until 1-2 days duration of culture, after 3-4 days they were died. The fused protoplasts regenerate cell wall in 1-2 days, while cell division and morphological changes were not observed.

更年期 障碍의 治療에 關한 硏究 : 中醫雜誌와 東義治療經驗集을 中心으로

閔丙和,崔銀洙,李仁仙 동의대학교 한의학연구소 1999 동의한의연구 Vol.3 No.-

This study has been carried out to investigate pathogenesis, symptoms, treatment method of Climacteric Hindrance. The results were obtained as follows ; 1. In oriental medicine, Oimacteric Hindrance is caused by exhaustion of chungye(天癸), discordance of internal organs, discordance of the roles of yin and yang in kidney and its pathogenesis are the deficiency of Qi of the kidney and the depression of liver energy. In western medicine, the decline of ovary function is regarded as Climacteric Hindrance's pathogenesis. 2. The symptoms of Climacteric Hindrance are divided into the whole body's symptoms(psychogenic symptom) and partial symptoms(menstruation hindrance). 3. The medical treatments of Climacteric Hindrance are medicine treatment, acupuncture and psychologic therapy. And the treatment method of Climacteric Hindrance are aliment liver and kidney, aliment um(陰) and blood, making mental comfort, aliment um(陰) and controlling fire, softening liver energy. 4. The symptoms of Climacteric Hindrance are different one another, so many treatment methods can be required.