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Bongsoo Choi,Do Young Hyeo,Juhun Lee,Terri A. Long,Daehee Hwang,Inhwan Hwang 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.5
E3 ligase BRUTUS (BTS), a putative iron sensor, is expressed in both root and shoot tissues in seedlings of Arabidopsis thaliana. The role of BTS in root tissues has been well established. However, its role in shoot tissues has been scarcely studied. Comparative transcriptome analysis with shoot and root tissues revealed that BTS is involved in regulating energy metabolism by modulating expression of mitochondrial and chloroplast genes in shoot tissues. Moreover, in shoot tissues of bts-1 plants, levels of ADP and ATP and the ratio of ADP/ATP were greatly increased with a concomitant decrease in levels of soluble sugar and starch. The decreased starch level in bts-1 shoot tissues was restored to the level of shoot tissues of wild-type plants upon vanadate treatment. Through this study, we expand the role of BTS to regulation of energy metabolism in the shoot in addition to its role of iron deficiency response in roots.
Bongsoo Lee,양지원,Gang-Guk Choi,Yoon-E. Choi,Minji Sung,Min S. Park 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.6
Microalgae-derived biomass has been considered as the most promising candidate for next generation biofueldue to its sustainability and biodegradability. In this study, microalgal strain Chlamydmonas reinhardtii was randomlymutagenized by using a chemical mutagen, ethyl methane sulfonate (EMS) to create mutants showing enhanced lipidproduction. We identified three random mutants that displayed high lipid production in the screening using Nile redstaining. Among those, mutant #128 was selected as candidate for further studies. Our flow cytometry and confocalmicroscopy analysis revealed that mutant #128 contains larger and more abundant lipid bodies than that of wild-type. Moreover, mutant #128 showed 1.4-fold increased fatty acid methyl ester (FAME) content compared to wild-type undernitrogen depleted condition. In addition, mutant #128 grew faster and accumulated more biomass, resulting in highlipid production. 2D gel electrophoresis and MALDI-TOF analysis used for gene targeting revealed that β-subunit ofmitochondrial ATP Synthase and two-component response regulator PilR may be involved in enhanced characteristicsof mutant #128. These results show the possibilities of EMS mediated random mutagenesis in generation of mutantsto produce high amount of lipid as well as further study for molecular mechanism of mutants.
Wafer-scale and patternable synthesis of NbS2 for electrodes of organic transistors and logic gates
Choi, Yongsuk,Bark, Hunyoung,Kang, Boseok,Lee, Myeongjae,Kim, BongSoo,Lee, Sungjoo,Lee, Changgu,Cho, Jeong Ho The Royal Society of Chemistry 2019 Journal of Materials Chemistry C Vol.7 No.28
<P>We developed a patternable synthesis method of wafer-scale NbS2, which can be applied for the fabrication of source and drain electrodes of p- and n-type organic field-effect transistors (OFETs) and logic gates. NbS2 film with high uniformity was synthesized directly on a 2-in Si wafer. NbS2 patterns of various sizes and shapes were readily synthesized onto an entire wafer. OFETs with NbS2 electrodes exhibited superior performances to those with conventional metal electrodes. The superior performance of the former OFETs was primarily a result of the enhanced crystallinity of the organic semiconductor layer deposited onto the NbS2 electrode surface. Furthermore, organic complementary circuits such as NOT, NAND, and NOR gates were successfully assembled using the resulting OFETs as a proof of applicability of these devices to complex logic circuits.</P>
Choi, Jong Yong,Kang, Woonggi,Kang, Boseok,Cha, Wonsuk,Son, Seon Kyoung,Yoon, Youngwoon,Kim, Hyunjung,Kang, Youngjong,Ko, Min Jae,Son, Hae Jung,Cho, Kilwon,Cho, Jeong Ho,Kim, BongSoo American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.10
<P>Bottom-contact bottom-gate organic field-effect transistors (OFETs) are fabricated using a low band gap pDTTDPP-DT polymer as a channel material and single-layer graphene (SLG) or Au source/drain electrodes. The SLG-based ambipolar OFETs significantly outperform the Au-based ambipolar OFETs, and thermal annealing effectively improves the carrier mobilities of the pDTTDPP-DT films. The difference is attributed to the following facts: (i) the thermally annealed pDTTDPP-DT chains on the SLG assume more crystalline features with an edge-on orientation as compared to the polymer chains on the Au, (ii) the morphological features of the thermally annealed pDTTDPP-DT films on the SLG electrodes are closer to the features of those on the gate dielectric layer, and (iii) the SLG electrode provides a flatter, more hydrophobic surface that is favorable for the polymer crystallization than the Au. In addition, the preferred carrier transport in each electrode-based OFET is associated with the HOMO/LUMO alignment relative to the Fermi level of the employed electrode. All of these experimental results consistently explain why the carrier mobilities of the SLG-based OFET are more than 10 times higher than those of the Au-based OTFT. This work demonstrates the strong dependence of ambipolar carrier transport on the source/drain electrode and annealing temperature.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-10/acsami.5b00747/production/images/medium/am-2015-00747f_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b00747'>ACS Electronic Supporting Info</A></P>
최정아,지은서,심지황,우애자,김봉수,최애란 이화여자대학교 교과교육연구소 2017 교과교육학연구 Vol.21 No.4
본 연구에서는 2014학년도부터 2017학년도의 중등화학교사신규임용후보자선정경쟁시험을 한국교육과정평가원에서 발표한 『2009학년도 표시과목 「화학」의 교사자격기준과 평가영역 및 평가내용요소』에 제시된 5개 기본 이수과목 및 분야의 평가영역과 평가내용요소를 준거로 분석하였다. 또한 중등화학교사임용시험 문항과 2009 개정 교육과정에 따른 성취기준과의 관련성도 파악하였다. 분석 결과 중등화학교사임용시험의 문항들이 설정된 평가영역과 평가내용요소들을 고르게 반영하고 있지 못하고 있음을 알 수 있었다. 또한 중등교육과정과 연계성이 있는 평가내용요소가 출제 비율이 낮거나, 매년 꾸준히 출제되는 문항들이지만 중등교육과정과 연계성이 적은 평가요소들이 다수 있었다. This study analyzed the examination for selecting secondary school chemistry teachers that was conducted from 2014 to 2017. As the analytic framework, we used the assessment domains and contents that were presented by the Korea Institute for Curriculum and Evaluation (KICE) for the five most basic prerequisite subjects for preservice chemistry teachers. We also identified relationship between the question items and the school chemistry/science curriculum. The results indicated that there were assessment domains and contents which were not involved in the question items of the examination for selecting secondary school chemistry teachers. Also, several question items were not relevant to the school chemistry/science curriculum.
황성원,Bongsoo Lee,Suk-Ho Choi 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.69 No.5
Nitride-based nanowires (NWs) have several advantages, such as flexibility in choosing a substrate, easy fabrication, large light-emitting area, no internal electric field, enhanced light extraction, and reduced defects by strain relief, that are useful for enhancing the efficiency of light-emitting diodes (LEDs). Here, we report how crucial the formation properties of the InGaN active layer are for enhancing the efficiency of core-shell InGaN/GaN multi-quantum-well (MQW)-NW LEDs that are selectively grown on oxide templates with perfectly-circular hole patterns. The nanostructures are analyzed for two types of LEDs, one containing defect-free MQW active layer and the other containing MQW layer with defects by using high-resolution transmission electron microscopy. The I-V curve of the defect-free LED shows a rectifying behavior with an on/off ratio of 109, typical of a diode, and the off-state leakage current of the LED with defects is much larger than that of the defect-free LED, resulting in brighter electroluminescence from the latter device. These results suggest that well-defined nonpolar InGaN/GaN MQW-NWs can be utilized for the realization of high-performance LEDs.