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Cu^2+-quinolone 복합체 존재하 DNA의 DNase Ⅰ에 대한 가수분해 반응성 증가
吳鳳京,徐正仁,柳聲元,高東成 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
특히, 그람음성 세균에 대한 활성을 가지며 Escherichia coli, Proteus 및 Klebsiella류의 감염에 의한 요도염(urinary tract infections)의 임상학적 치료에 유효함이 증명된1-5 합성 항균제인 nalidixic acid(lethy1-1,4-dihydro-7-methyl-4-oxo-1,8-naphthyridine-3-carboxylic acid)를 비롯한 quinolones 항균제의 개발연구가6 국내외 공히 활발하게 진해되고 있음에도 불구하고 그 작용 메커니즘이 제대로 밝혀지지 못하고있는 실정이다. 이들은 DNA합성을 방해 하므로써 향균 기능을 갖는다고 알려져 있으나 그 표적물(target)이 무엇인가에 대하여는 불분명하다. 이들 quinolones는 생리적 pH에서 음이온으로 존재하므로 정전기적 반발로 인하여 다음이온성(polyanionic)인 DNA와의 직접적 상호작용 가능성이 배제되어 왔으며 일반적인 견해는 DNA gyrase subunit A가 nalidixic acid의 특이한 target인 것으로 알려져 있다.
신경회로망 제어기를 이용한 PID 파라미터 추정에 관한 연구
權重東,裵銀敬,金恩基,全基英,李承桓,吳鳳煥,李勳九,金容珠,韓慶熙 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-
In this paper, supposed to solve these problem to PID parameters controller algorithm using ANN. In the proposed algorithm, the parameters of the controller were adjusted to reduce by on-line system the error of the speed of IM. In this process, EBPA NN was constituted to an output error value of an IM and conspired an input and output. The performance of the self-tuning controller is compared with that of the PH) controller tuned by conventional method (Ziehler-Nichols). The effectiveness of the proposed control method is verified thought the Matlab Simulink and experimental results.
강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-
MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.
Oh, Bong-Kyeong,Ryu, Hyeong-Won,Ko, Thong-Sung 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
본 연구에서 환원시킨 nalidixic acid가 송아지 흉선 DNA의 DNase 1에 대한 가수분해 활성을 증가시킴을 알았다. 이 결과는 nalidixic acid가 환원되면 DNA intercalation 가능 형태로 전환됨을 시사한다. We have prepared reduced nalidixic acid and have shown the enhancing effect of the reduced nalidixic acid on the susceptibility of calf thymus DNA to DNase 1. The data have been interpreted to indicate the possibility that quinolone can be converted to DNA intercalative form after being reduced.
Oh, Bong-Kyeong,Kim, Young-Joo,Park, Young Nyun,Choi, Jinsub,Kim, Kyung Sik,Park, Chanil American College of Gastroenterology 2006 The American journal of gastroenterology Vol.101 No.4
<P>BACKGROUND: Telomerase reverse transcriptase (hTERT) is the rate-limiting determinant of telomerase, which is critical for carcinogenesis. Dysplastic nodules (DNs) appear to be preneoplastic lesions of hepatocellular carcinomas (HCCs). In this study, in order to characterize DNs, hTERT mRNA, hTERT gene dosage, and mRNA for c-myc, a transcriptional activator of hTERT were studied in human multi-step hepatocarcinogenesis. METHODS: Fifty four hepatic nodules including 5 large regenerative nodules, 14 low-grade DNs, 7 high-grade DNs, 11 DNs with HCC foci and 17 HCCs, 23 livers with chronic hepatitis/cirrhosis, and 6 normal livers were examined. Transcript levels were measured by real-time quantitative RT-PCR and gene dosages by real-time PCR and Southern blotting. RESULTS: The hTERT mRNA levels increased with the progression of hepatocarcinogenesis, and a significant induction in the transition between low- and high-grade DNs was seen. Most high-grade DNs strongly expressed hTERT mRNA at levels similar to those of HCCs. Twenty-one percent of low-grade DNs had high levels of hTERT mRNA, up to those of high-grade DNs and there was no difference in the pathological features between low-grade DNs with and without increased hTERT mRNA levels. No correlation was found between hTERT mRNA levels, hTERT gene dosage, and c-myc mRNA levels. CONCLUSIONS: These results suggest that the induction of hTERT mRNA is an important early event and that its measurement by real-time quantitative RT-PCR is a useful tool to detect premalignant/malignant tendencies in hepatic nodules. However, hTERT gene dosage and c-myc expression are not the main mechanisms regulating hTERT expression in hepatocarcinogenesis.</P>
Variable TERRA abundance and stability in cervical cancer cells
Oh, Bong-Kyeong,Keo, Ponnarath,Bae, Jaeman,Ko, Jung Hwa,Choi, Joong Sub Spandidos Publications 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.6
<P>Telomeres are transcribed into long non-coding RNA, referred to as telomeric repeat-containing RNA (TERRA), which plays important roles in maintaining telomere integrity and heterochromatin formation. TERRA has been well characterized in HeLa cells, a type of cervical cancer cell. However, TERRA abundance and stability have not been examined in other cervical cancer cells, at least to the best of our knowledge. Thus, in this study, we measured TERRA levels and stability, as well as telomere length in 6 cervical cancer cell lines, HeLa, SiHa, CaSki, HeLa S3, C-33A and SNU-17. We also examined the association between the TERRA level and its stability and telomere length. We found that the TERRA level was several fold greater in the SiHa, CaSki, HeLa S3, C-33A and SNU-17 cells, than in the HeLa cells. An RNA stability assay of actinomycin D-treated cells revealed that TERRA had a short half-life of similar to 4 h in HeLa cells, which was consistent with previous studies, but was more stable with a longer half-life (>8 h) in the other 5 cell lines. Telomere length varied from 4 to 9 kb in the cells and did not correlate significantly with the TERRA level. On the whole, our data indicate that TERRA abundance and stability vary between different types of cervical cancer cells. TERRA degrades rapidly in HeLa cells, but is maintained stably in other cervical cancer cells that accumulate higher levels of TERRA. TERRA abundance is associated with the stability of RNA in cervical cancer cells, but is unlikely associated with telomere length.</P>
Bong Seob Yang,Seungha Oh,Yoon Jang Kim,Sang Jin Han,Hong Woo Lee,Hyuk Jin Kim,Sungmin Kim,Hui Kyung Park,Jaeyeong Heo,Jae Kyeong Jeong,Hyeong Joon Kim IEEE 2014 IEEE transactions on electron devices Vol.61 No.6
<P>The oxygen ratio-dependent device performance and reliability of zinc tin oxide (ZTO) thin-film transistors (TFTs) were examined. ZTO TFTs fabricated under pure Ar conditions exhibited a high saturation mobility of 21.5 cm<SUP>2</SUP>/Vs, low subthreshold gate swing of 0.34 V/decade, and high I<SUB>ON/OFF</SUB> ratio (10<SUP>9</SUP>), whereas modest mobility of 7.3 cm<SUP>2</SUP>/Vs was obtained for the ZTO TFTs prepared at an oxygen ratio [R = O<SUB>2</SUB>/(Ar + O<SUB>2</SUB>)] of 0.3. The photobias stability (AV<SUB>th</SUB> ≈-2.0 V) of the ZTO TFTs under the Ar only condition was much better than that (AV<SUB>th</SUB> ≈-5.9 V) of the device at R of 0.3, even though their channel layers contained the larger density of oxygen vacancies. This abnormal behavior was attributed to the compressive stress of ZTO films retarding the phototransition of oxygen vacancies.</P>
Kyeong Ho Song,Won Seok Oh,Jae Woo Lee,Min Wook Kim,Dae Kyun Jeong,Seong Hwan Bae,Hyun Yul Kim,Youn Joo Jung,Ki Seok Choo,Kyung Jin Nam,Ji Hyeon Joo,Mi Sook Yun,Su Bong Nam 대한성형외과학회 2021 Archives of Plastic Surgery Vol.48 No.6
Background Breast reconstruction using an extended latissimus dorsi (eLD) flap can supplement more volume than reconstruction using various local flaps after partial mastectomy, and it is a valuable surgical method since the reconstruction area is not limited. However, when performing reconstruction, the surgeon should consider latissimus dorsi (LD) volume reduction due to postoperative chemotherapy (POCTx) and postoperative radiotherapy (PORTx). To evaluate the effect of POCTx and PORTx on LD volume reduction, the effects of each therapy—both separately and jointly—need to be demonstrated. The present study quantified LD volume reduction in patients who underwent POCTx and PORTx after receiving breast-conserving surgery (BCS) with an eLD flap. Methods This study included 48 patients who received immediate breast reconstruction using an eLD flap from January 2013 to March 2017, had chest computed tomography (CT) 7–10 days after surgery and 10–14 months after radiotherapy completion, and were observed for more than 3 years postoperatively. One surgeon performed the breast reconstruction procedures, and measurements of breast volume were obtained from axial CT views, using a picture archiving and communication system. A P-value <0.05 was the threshold for statistical significance. Results The average volume reduction of LD at 10–14 months after completing POCTx and PORTx was 64.5% (range, 42.8%–81.4%) in comparison to the volume measured 7–10 days after surgery. This change was statistically significant (P<0.05). Conclusions Based on the findings of this study, when harvesting an eLD flap, surgeons should anticipate an average LD volume reduction of 64.5% if chemotherapy and radiotherapy are scheduled after BCS with an eLD flap.