Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

Wang Laiyou,Guo Shuxian,Zeng Bo,Wang Shanshan,Chen Yan,Cheng Shuang,Liu Bingbing,Wang Chunyan,Wang Yu,Meng Qingshan 한국균학회 2022 Mycobiology Vol.50 No.1

The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key eco- nomic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequenc- ing data or associated annotations for this strain are available at present, it remains chal- lenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic com- plete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mech- anistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

Insights into interfacial stability of Li6PS5Cl solid electrolytes with buffer layers

Bingbing Chen,Chaoqun Xu,Han Wang,Jianqiu Zhou 한국물리학회 2019 Current Applied Physics Vol.19 No.2

The large interfacial resistance seriously restricts the development of all-solid-state lithium batteries (ASSLBs). In our work, first-principles calculations are employed to investigate the interfacial properties on lithium (Li) metal anode/Li6PS5Cl solid electrolyte (LPSCl) interface system as well as buffer layers (Li2S) effects. The stable interface structures, Li/LPSCl, L2S/LPSCl and Li/L2S, are established at atomic level. We find that PS4 tetrahedral structure has been seriously destroyed in Li/LPSCl interface, whereas the presence of Li2S buffer layers may smooth the interface without PS4 tetrahedral damage occurred. In addition, the electronic structure of interface indicates that solid electrolyte interphases are not easy to form on LPSCl surfaces considering buffer layers effects, which may improve the stability of anode/solid electrode interface. Moreover, the calculated energies of exchange ions between Li metal and solid electrolyte with buffer layers suggest that the Li2S interposition can suppress the atoms diffusion in LPSCl layers, and provide a smooth interface structure, which may promote the stability of Li/LPSCl interface. This work on the atomic scale will offer a useful perspective for designing high performance of solid electrolytes to enhance good cyclability in ASSLBs.

Path Following of Underactuated Unmanned Surface Vehicle Based on Trajectory Linearization Control with Input Saturation and External Disturbances

Bingbing Qiu,Guo-Feng Wang,Yun-Sheng Fan,Dong-Dong Mu,Xiaojie Sun 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.8

This paper investigates the path following control problem for underactuated unmanned surface vehicle(USV) in the presence of unmodeled dynamics, external disturbances and input saturation. A novel adaptive robustpath following control scheme is proposed by employing trajectory linearization control (TLC) technology andfinite-time disturbance observer, which is composed of a concise yaw rate controller and a surge speed controller. The salient features of the proposed scheme include: a path following guidance law is designed to ensure USVeffectively converging to and following the desired path; TLC is introduced into the field of USV motion controlas new effective technique, and it is the first time used to design path following controller for underactuated USV;a finite-time nonlinear tracking differentiator is constructed not only to avoid the signal jump caused by derivation,but also to filter noise and high frequency interference. A finite-time disturbance observer (FDO) is devised toexactly observe the uncertain dynamics and unknown external disturbances, which improves the tracking accuracyand precise disturbance rejection of the proposed controller; then, an auxiliary dynamic system that is governed bysmooth switching function is developed to compensate for the saturation constraint on actuator. Stability analysisverifies that all signals in the closed-loop system are uniformly ultimately bounded. Finally, simulation results andcomparisons illustrate the superiority of the proposed control scheme.

Inhibition of Wnt/β-catenin signaling by monensin in cervical cancer

Fu Bingbing,Fang Lixia,Wang Ranran,Zhang Xueling 대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.1

The challenging clinical outcomes associated with advanced cervical cancer underscore the need for a novel therapeutic approach. Monensin, a polyether antibiotic, has recently emerged as a promising candidate with anti-cancer properties. In line with these ongoing efforts, our study presents compelling evidence of monensin's potent efficacy in cervical cancer. Monensin exerts a pronounced inhibitory impact on proliferation and anchorage-independent growth. Additionally,monensin significantly inhibited cervical cancer growth in vivo without causing any discernible toxicity in mice. Mechanism studies show that monensin's anti-cervical cancer activity can be attributed to its capacity to inhibit the Wnt/β -catenin pathway, rather than inducing oxidative stress. Monensin effectively reduces both the levels and activity of β -catenin, and we identify Akt, rather than CK1, as the key player in-volved in monensin-mediated Wnt/β-catenin inhibition. Rescue studies using Wnt activator and β-catenin-overexpressing cells confirmed that β-catenin inhibition is the mechanism of monensin’s action. As expected, cervical cancer cells exhibiting heightened Wnt/ β-catenin activity display increased sensitivity to monensin treat-ment. In conclusion, our findings provide pre-clinical evidence that supports further exploration of monensin's potential for repurposing in cervical cancer therapy, par - ticularly for patients exhibiting aberrant Wnt/ β -catenin activation

Dynamic threshold location algorithm based on fingerprinting method

Xuxing Ding,Bingbing Wang,Zaijian Wang 한국전자통신연구원 2018 ETRI Journal Vol.40 No.4

The weighted K‐nearest neighbor (WKNN) algorithm is used to reduce positioning accuracy, as it uses a fixed number of neighbors to estimate the position. In this paper, we propose a dynamic threshold location algorithm (DH‐KNN) to improve positioning accuracy. The proposed algorithm is designed based on a dynamic threshold to determine the number of neighbors and filter out singular reference points (RPs). We compare its performance with the WKNN and Enhanced K‐Nearest Neighbor (EKNN) algorithms in test spaces of networks with dimensions of 20 m × 20 m, 30 m × 30 m, 40 m × 40 m and 50 m × 50 m. Simulation results show that the maximum position accuracy of DH‐KNN improves by 31.1%, and its maximum position error decreases by 23.5%. The results demonstrate that our proposed method achieves better performance than other well‐known algorithms.

DPT (Data Process Tracking) Analysis of Biopharmaceutical Companies Based on fsQCA Method of ESG System: Aiming at Improving the ESG System MES-DPT & GMP-DPT and the ESG Quantitative Indicators of the Biopharmaceutical Industry

Gao Yu,Wang Lingyan,Qin Bingbing 한중경제문화학회 2023 한중경제문화연구 Vol.24 No.-

In recent years, safety recalls have occurred frequently in the biopharmaceutical industry, which affects the health of consumers. This article attempts to use the fsQCA method to draw a conclusion through the study of ESG and its quantification system, as well as the study of data samples related to MES and GMP processes, that is, MES-DPT has a positive impact on process safety management, and GMP-DPT has a positive impact on process safety. Management has a positive and positive impact, and ESG-DPT has a positive and positive impact on process safety management. Finally, this article puts forward suggestions for improving ESG-DPT, MES-DPT, GMP-DPT and the biomedical ESG-DPT model. Future research hopes to further study ESG -DPT model and ESG biomedical industry indicators.

Omega model for a jump–diffusion process with a two-step premium rate

Jingmin He,Zhongqin Gao,Bingbing Wang 한국통계학회 2019 Journal of the Korean Statistical Society Vol.48 No.3

In this paper, a jump–diffusion Omega model with a two-step premium rate is studied. In this model, the surplus process is a perturbation of a compound Poisson process by a Brown motion. Firstly, using the strong Markov property, the integro-differential equations for the Gerber–Shiu expected discounted penalty function and the bankruptcy probability are derived. Secondly, for a constant bankruptcy rate function, the renewal equations satisfied by the Gerber–Shiu expected discounted penalty function are obtained, and by iteration, the closed-form solutions of the function are also given. Further, the explicit solutions of the Gerber–Shiu expected discounted penalty function are obtained when the individual claim size is subject to exponential distribution. Finally, a numerical example is presented to illustrate some properties of the model.

Modeling and dynamic analysis of artillery barrel-cradle structure with clearance

Jie Liu,Changda Wang,Bingbing Hu,Bing Li 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.4

Clearance is unavoidable in artillery barrel-cradle mechanism due to the need for relative motion, and has been considered as a key factor to affect the artillery firing accuracy. Therefore, in this paper, the dynamic behaviors of artillery barrel-cradle structure are studied. The barrel-cradle structure is simplified as a variable cross-section barrel structure with two clearances, and nonlinear spring-damping model is used to describe the clearance contact force. And then, based on solving the dynamic response of such model, the clearance effects on muzzle vibration, contact force and dynamic characteristics (frequency response function and “hardening” effect) are also analyzed in detail. Furthermore, this paper also presents an improved identification algorithm which is combining the probability density derivative method and nonlinear detection to identify the clearance value. These studies can provide better understanding of clearance effects and contribute significantly to improving firing accuracy by adjusting the clearance.

Characteristics of calcium carbonate fouling on heat transfer surfaces under the action of electric fields

Zhiming Xu,Hongliang Chang,Bingbing Wang,Jingtao Wang,Qi Zhao 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.7

The present study examined the effect of electric fields in calcium carbonate (CaCO 3 ) scale formation on a heat transfer surface. The effects of electric fields ranging from 0 V to 4000 V on the fouling properties of CaCO 3 were investigated. Results showed that the optimal electric voltage was approximately 500 V, at which the asymptotic value of fouling resistance and the deposited weight were minimal and corresponded to 52.8 % and 61.3 % reductions, respectively, compared with the results recorded at 0 V. At higher voltages of 3000 V or 4000 V, the asymptotic value of fouling resistance and the weight of fouling deposits increased relative to those obtained at 0 V. The scanning electron microscope images of the fouling deposits obtained at 0 V showed mainly aragonites with sharp and needle-like crystal structures. The structure of CaCO 3 fouling changed from aragonites to spherical vaterites as the applied voltage was increased.

Prognostic Value of 18F-FDG PET/CT Radiomics in Extranodal Nasal-Type NK/T Cell Lymphoma

Luo Yu,Huang Zhun,Gao Zihan,Wang Bingbing,Zhang Yanwei,Bai Yan,Wu Qingxia,Wang Meiyun 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.2

Objective: To investigate the prognostic utility of radiomics features extracted from 18F-fluorodeoxyglucose (FDG) PET/CT combined with clinical factors and metabolic parameters in predicting progression-free survival (PFS) and overall survival (OS) in individuals diagnosed with extranodal nasal-type NK/T cell lymphoma (ENKTCL). Materials and Methods: A total of 126 adults with ENKTCL who underwent 18F-FDG PET/CT examination before treatment were retrospectively included and randomly divided into training (n = 88) and validation cohorts (n = 38) at a ratio of 7:3. Least absolute shrinkage and selection operation Cox regression analysis was used to select the best radiomics features and calculate each patient’s radiomics scores (RadPFS and RadOS). Kaplan–Meier curve and Log-rank test were used to compare survival between patient groups risk-stratified by the radiomics scores. Various models to predict PFS and OS were constructed, including clinical, metabolic, clinical + metabolic, and clinical + metabolic + radiomics models. The discriminative ability of each model was evaluated using Harrell’s C index. The performance of each model in predicting PFS and OS for 1-, 3-, and 5-years was evaluated using the time-dependent receiver operating characteristic (ROC) curve. Results: Kaplan–Meier curve analysis demonstrated that the radiomics scores effectively identified high- and low-risk patients (all P < 0.05). Multivariable Cox analysis showed that the Ann Arbor stage, maximum standardized uptake value (SUVmax), and RadPFS were independent risk factors associated with PFS. Further, β2-microglobulin, Eastern Cooperative Oncology Group performance status score, SUVmax, and RadOS were independent risk factors for OS. The clinical + metabolic + radiomics model exhibited the greatest discriminative ability for both PFS (Harrell’s C-index: 0.805 in the validation cohort) and OS (Harrell’s C-index: 0.833 in the validation cohort). The time-dependent ROC analysis indicated that the clinical + metabolic + radiomics model had the best predictive performance. Conclusion: The PET/CT-based clinical + metabolic + radiomics model can enhance prognostication among patients with ENKTCL and may be a non-invasive and efficient risk stratification tool for clinical practice.