3D Animation Skills Training Research based on Performance Technology

Ma Ben(마분),Hyun-Cheol Lee(이현철),Eun-Seok Kim(김은석),GI-Taek Hur(허기택) 한국콘텐츠학회 2011 한국콘텐츠학회 종합학술대회 논문집 Vol.9 No.1

Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

Lan-Qing Ma,Yan-Wu Guo,Hui-Li Guo,Xing Li,Li-Li Huang,Bo-Ning Zhang,Xiao-Bin Pang,Ben-Ye Liu,Hong Wang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

In our recent work (Ma et al., in Planta229(3):457–469, 2009a and 229(4):1077–1086, 2009b),two three-intron type III PKS genes, PcPKS1 and PcPKS2,were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is athree-intron chalcone synthase (CHS) gene, and PcPKS2 isfound to be a three-intron benzalacetone synthase (BAS)gene. The regular CHS encoded by a single intron genehave not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3)and a stilbene synthase (STS) gene with three-intron(PcPKS5) were isolated and characterized by functionaland phylogenetic analyses. In comparison with PcPKS1, abifunctional enzyme with both CHS and BAS activity, theenzymatic product of recombinant PcPKS3 was naringenin,bis-noryangonin (BNY) and 4-coumaroyltriacetic acidlactone (CTAL) occurred as side products. The PcPKS5synthesized resveratrol and a trace amount of naringeninfrom p-coumaroyl-CoA. To our knowledge, PcPKS5 is thefirst reported three-intron STS gene in flowering plants. Inthis work, we speculated that this involved a possibleevolutionary route of plant-specific type III PKS superfamilyin P. cuspidatum.

Enhanced electrical conductivity of nanocomposites containing hybrid fillers of carbon nanotubes and carbon black.

Ma, Peng-Cheng,Liu, Ming-Yang,Zhang, Hao,Wang, Sheng-Qi,Wang, Rui,Wang, Kai,Wong, Yiu-Kei,Tang, Ben-Zhong,Hong, Soon-Hyung,Paik, Kyung-Wook,Kim, Jang-Kyo American Chemical Society 2009 ACS APPLIED MATERIALS & INTERFACES Vol.1 No.5

<P>Nanocomposites reinforced with hybrid fillers of carbon nanotubes (CNTs) and carbon black (CB) are developed, aiming at enhancing the electrical conductivity of composites with balanced mechanical properties while lowering the cost of the final product. Epoxy-based nanocomposites were prepared with varying combinations of CNTs and CB as conducting fillers, and their electrical and mechanical properties were evaluated. It was shown that the addition of CNTs in CB composites enhanced the electrical conductivity of composites: a low percolation threshold was achieved with 0.2 wt % CNTs and 0.2 wt % CB particles. The CB particles also enhanced the ductility and fracture toughness of nanocomposites, confirming the synergistic effect of CB as a multifunctional filler. The novelty of this work lies in the synergy arising from the combination of two conducting fillers with unique geometric shapes and aspect ratios as well as different dispersion characteristics, which have not been specifically considered previously.</P>

Thermo- and pH-Responsive CeF3 Nanocrystals/HPC–PAA Complex Nanogel and Its Drug Release Behavior

Ben Liu,Qiusheng Song,Qiancheng Zhang,Haihong Ma,Ziwen Zhang,Dapeng Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.8

In this study, a kind of novel rare-earth nanocrystals/hydroxypropylcellulose–poly (acrylic acid) (HPC–PAA) complex fluorescent nanogel, its responsive behavior to environmental temperature and pH value were reported. For preparation, with HPC being used as template, HPC–PAA nanogel was first synthesized by polymerization and crosslinking reaction of acrylic acid (AA) in water solution. In the process, a redox initiator, which composed of ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED), was used to initiate the reaction. Then, the as-prepared nanogel reacted with Ce(NO3)3 and NH4F solution successively, and a novel CeF3 nanocrystals/HPC–PAA complex nanogel was fabricated. The microstructure of the nanogel was characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), Thermogravimetric (TG) and Transmission electron microscopy (TEM). The environmental sensitivity of the nanogel was investigated by photoluminescence (PL) and UV–Visible spectrophotometer (UV–Vis). The thermo- and pH-sensitive fluorescence were studied by PL at various temperatures and pH values; besides, the behavior of drug loading and release was researched by PL with a famous antibiotic of Ibuprofen as model drug. The results show that the PL intensity of the nanogel was largely affected by environmental temperature, or content of Ibuprofen loaded in the nanogel. The as-prepared nanogel can be used as useful sensitive material to detect temperature and pH value change, and drug loading or release property of Ibuprofen can be detected by PL emission of the nanogels conveniently.

Aberrant methylation of the 16q23.1 tumor suppressor gene ADAMTS18 promotes tumorigenesis and progression of clear cell renal cell carcinoma

Ben Xu,Yi‑ji Peng,Bing‑lei Ma,Si‑da Cheng 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background The 16q23.1 tumor suppressor gene (TSG) of ADAMTS18 has been identifed to be aberrant methylated in clear cell renal cell carcinoma (ccRCC), and there still exists an unclear situation between its methylation and the progression of ccRCC. Objective To analyze the biological function and mechanism of ADAMTS18 gene in the tumorigenesis and progression of ccRCC. Methods We examined ADAMTS18 gene methylation using methylation- specifc polymerase chain reaction (MSP) in 92 ccRCC primary tumors from September 2017 to May 2018. Using reverse transcriptase PCR (RT-PCR) and immunohistochemical (IHC) assay, the relative expression level of ADAMTS18 was measured in the representative tumor samples with their adjacent normal tissues. Meanwhile, colony formation, cell viability, wound healing, transwell chamber, fow cytometry, and PI staining were performed to confrm the tumor-suppressive function and mechanism of ADAMTS18 gene. Results Aberrant methylation was further detected in 47 of the 92 (51.1%) primary tumors and in 8 of the 92 (8.7%) adjacent normal tissues (p<0.05). Due to the phenomenon of aberrant methylation, ectopic low-level expression of ADAMTS18 gene could result in the promotion of tumorigenesis and progression in ccRCC. Conclusion The aberrantly methylated ADAMTS18 gene may be involved in the tumorigenesis and progression of ccRCC.

Expression and Characterization of a New L-amino Acid Oxidase AAO Producing α-ketoglutaric Acid from L-glutamic Acid

Rao Ben,Liao Xianqing,Liu Fang,Chen Wei,Zhou Ronghua,Ma Lixin,Wang YaPing 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.6

L-amino acid oxidase (AAO) was reported to be capable of converting L-glutamic acid to α-aketoglutaric acid (α-KG). The sequence of AAO from Kitasatospora cheerisanensis was synthesized based on Pichia pastoris codon-usage preferences. AAO gene was cloned into plasmid pPICZα which was transformed into P. pastoris. Next, multi-copy expression plasmids were constructed by using plasmid pHBM905BDM. High-density fermentation was performed and the recombinant enzyme was characterized. The conversion conditions were optimized. By using Escherichia coli expression system, no soluble or active AAO was obtained from two strains after fermentation and induction. We can’t obtain high-level expression of recombinant strains by using plasmid pPICZα. Therefore, we constructed multi-copy expression plasmids using plasmid pHBM905BDM. By using this plasmid, multi-copy strains were constructed and named as PAAO1, PAAO2, PAAO3, PAAO4, and PAAO5, respectively. The following results showed that expression of AAO in multicopy strains increased as designed and strain PAAO5 was chosen for high-density fermentation and enzyme activity experiments. After high-density fermentation, we achieved an AAO-expression yield of 120.8 U/mL. After temperature and pH optimization, the highest AAO activity was observed at a temperature and pH of 20°C and 6, respectively. After optimization of the conversion conditions, the average production rate of L-glutamic acid to α-KG was 3.46 g/L/h and the highest α-KG titer (103 g/L) was converted from 120 g/L L-glutamic acid. In this study, AAO was abundantly expressed by using P. pastoris expression system. The following experiments indicated that AAO is suitable for use in industrial applications.

Fault-tolerant Consensus of Leader-following Multi-agent Systems Based on Distributed Fault Estimation Observer

Pu Yang,Ben Ma,Yan Dong,Jianwei Liu 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.5

This paper is concerned with the fault-tolerant consensus control problem for leader-following multiagent systems with actuator faults and external disturbance. Firstly, by employing the relative output estimation errors with respect to the corresponding neighbours and the leader, a distributed fault estimation observer is constructed for each follower and the established global estimation error system is proven to be asymptotically stable. Secondly, based on the obtained fault estimation, the active fault-tolerant consensus control protocol for each follower is proposed with the utilization of the relative output measurements rather than the state of the systems. Under this control protocol, the actuator faults can be compensated and the influence of disturbance can be eliminated as well. Moreover, it is worth noticing that the process of designing the fault estimation observer and the fault-tolerant controller is separated and their performances are considered simultaneously to simplify the design procedures. Finally, an illustrative example is presented to demonstrate the applicability and the effectiveness of the obtained results.

Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with Colorectal Cancer Susceptibility

Du, Wei,Ma, Xue-Lei,Zhao, Chong,Liu, Tao,Du, Yu-Liang,Kong, Wei-Qi,Wei, Ben-Ling,Yu, Jia-Yun,Li, Yan-Yan,Huang, Jing-Wen,Li, Zi-Kang,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Background: MicroRNAs (miRNAs) are an abundant class of endogenous small non-coding RNAs of 20-25 nucleotides in length that function as negative gene regulators. MiRNAs play roles in most biological processes, as well as diverse human diseases including cancer. Recently, many studies investigated the association between SNPs in miR-146a rs2910164, miR-196a2 rs11614913, miR-149 rs229283, miR-499 rs3746444 and colorectal cancer (CRC), which results have been inconclusive. Methodology/Principal Findings: PubMed, EMBASE, CNKI databases were searched with the last search updated on November 5, 2013. For miR-196a2 rs11614913, a significantly decreased risk of CRC development was observed under three genetic models (dominant model: OR = 0.848, 95%CI: 0.735-0.979, P = 0.025; recessive model: OR = 0.838, 95%CI: 0.721-0.974, P = 0.021; homozygous model: OR = 0.754, 95%CI: 0.627-0.907, P = 0.003). In the subgroup analyses, miR-$196a2^*T$ variant was associated with a significantly decreased susceptibility of CRC (allele model: OR = 0.839, 95%CI: 0.749-0.940, P = 0.000; dominant model: OR = 0.770, 95%CI: 0.653-0.980, P = 0.002; recessive model: OR = 0.802, 95%CI: 0.685-0.939, P = 0.006; homozygous model: OR = 0.695, 95%CI: 0.570-0.847, P = 0.000). As for miR-149 rs2292832, the two genetic models (recessive model: OR = 1.199, 95% CI 1.028-1.398, P = 0.021; heterozygous model: OR = 1.226, 95% CI 1.039-1.447, P = 0.013) demonstrated increased susceptibility to CRC. On subgroup analysis, significantly increased susceptibility of CRC was found in the genetic models (recessive model: OR = 1.180, 95% CI 1.008-1.382, P = 0.040; heterozygous model: OR = 1.202, 95% CI 1.013-1.425, P = 0.013) in the Asian group. Conclusions: These findings supported that the miR-196a2 rs11614913 and miR-149 rs2292832 polymorphisms may contribute to susceptibility to CRC.

Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,Huang, Li-Li,Zhang, Bo-Ning,Pang, Xiao-Bin,Liu, Ben-Ye,Ma, Lan-Qing,Wang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.

Flexural behavior of ultra high performance concrete beams reinforced with high strength steel

Jun-Yan Wang,Jin-Ben Gu,Chao Liu,Yu-Hao Huang,Ru-Cheng Xiao,Biao Ma 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.81 No.5

A detailed experimental program was conducted to investigate the flexural behavior of ultra high performance concrete (UHPC) beams reinforced with high strength steel (HSS) rebars with a specified yield strength of 600 MPa via direct tensile test and monotonic four-point bending test. First, two sets of direct tensile test specimens, with the same reinforcement ratio but different yield strength of reinforcement, were fabricated and tested. Subsequently, six simply supported beams, including two plain UHPC beams and four reinforced UHPC beams, were prepared and tested under four-point bending load. The results showed that the balanced-reinforced UHPC beams reinforced with HSS rebars could improve the ultimate loadbearing capacity, deformation capacity, ductility properties, etc. more effectively owing to interaction between high strength of HSS rebar and strain-hardening characteristic of UHPC. In addition, the UHPC with steel rebars kept strain compatibility prior to the yielding of the steel rebar, further satisfied the plane-section assumption. Most importantly, the crack pattern of the UHPC beam reinforced with HSS rebars was prone to transform from single main crack failure corresponding to the normal-strength steel, to multiple main cracks failure under the condition of balanced-reinforced failure, which validated by the conclusion of direct tensile tests cooperated with acoustic emission (AE) source locating technique as well.