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미세현미조작된 생쥐배의 발생과 단일할구의 성감별 기술에 관한 연구 2 . 미세조작에 의해 분리된 할구의 염색체 분석조건에 관한 연구
김익수(I . S . Jeon),정범식(B . S . Jung),박수봉(S . B . Park),최광수(K . S . Choi) 한국축산학회 1991 한국축산학회지 Vol.33 No.7
The study was carried out to investigate the optimum condition of C-banding for sexing mouse embryo by chromosome analysis of single blastomere. The sexing rate was 42.9% with the 114 blastocyst embryos which were used C-banding after being hyp-otonic-treated in 1% sodium citrate for 30 minutes. The separated blastomeres for 4-cell embryos were used for C-banding after being hypotonic-treated in 1% sodium citrate for 20-30, 10-15 and 1-2 min., and the sexing rate for different treatment times were 5.5%. 0%, and 0%, respectively. The separated blastomeres from 4-cell embryos were used for C-banding after being hypotonic-treated in 30% fetal calf serum for 20-30, 10-15 and 1-2 min., and the sexing rate for different treatment times were 18.5%, 0% and 0% respectively.
전익수(I . S . Jeon),김선의(S . E . Kim),정진관(J . K . Jung),정일정(I . C . Cheong),박영식(Y . S . Park),최광수(K . S . Choi) 한국축산학회 1993 한국축산학회지 Vol.35 No.6
The effect of micromanipulation and zona pellucida(ZP) on in vitro viability and development of frozen embryos was investigated for the effective cryopreservation of biopised embryos. The ZP-intact 4-cell embryos and ZP-removed 4-cell embryos were biopised using micromanipulator and micropipette, respectively. Biopsied embryos were equilibrated in M16 containing 3.5M DMSO and 0.25M sucrose for 1.5 minutes and then were ultrarapidly frozen in LN₂. The frozen embryos were thawed at 37℃ and were cultured in M 16 at 37℃ for 48 hours under an atmosphere of 5% CO₂ and 100% humidity. The developmental rate of fresh and frozen embryos was evaluated as percentage of embryo development from 4 cell stage to blastocyst. The results were summarized as: 1) the survival rate of 4-cell embryos ultrarapidly frozen and thawed was 73%. The development rate of survived embryos after freezing was 92%; 2) the survival rate of micromanipulated-embryos ultrarapidly frozen and thawed was 79?. The development rate of survived embryos after freezing was 59%; 3) the developmental rate of the cultured fresh ZP-intact embryos was significantly higher than those of the fresh ZP-removed embryos and fresh ZP-removed biopsied embryos (p$lt;.05); and 4) the developmental rate of the cultured frozen ZP-intact embryos was significantly higher than those of the frozen ZP-removed embryos and frozen ZP-removed biopsied embryos(p$lt;.05).
돼지의 혈청 Insulin-like Growth Factor-I과 산자수간의 연관성 연구
양성호,서동삼,박희복,김기동,강창원,최광수,박성수,홍기창,고용,Yang, S.H.,Seo, D.S.,Park, H.B.,Kim, K.D.,Kang, C.W.,Choi, K.S.,Park, S.S.,Hong, K.C.,Ko, Y. 한국동물번식학회 1999 Reproductive & developmental biology Vol.23 No.3
The litter size has been the primary interest of economic traits in pig reproduction. It has been recently shown that insulin-like growth factor-Ⅰ(IGF-Ⅰ) plays roles in establishing pregnancy and in supporting fetal growth and development. But, the effect of serum IGF-Ⅰ on litter size has not been studied. Therefore, this study was conducted to relate serum IGF-Ⅰ concentration with porcine litter size and to investigate the possible connection with estrogen receptor(ER) as a candidate gene for the litter size. Sera during day 45 to 105 of pregnancy were collected from two groups showing high and low litter size and serum IGF-Ⅰ concentration was measured by radioimmunoassay (RIA). IGF-Ⅰ levels in both groups decreased gradually as pregnancy stage proceeded but were not significantly different. Secondly, DNA was extracted from blood and PCR-RFLP was utilized to analyze ER genotypes of pigs in each group, which produced three polymorphic patterns. Based on the ER genotypes analyzed, low litter size group showed higher IGF-Ⅰ concentration than high litter size group. Taken together, the results indicate that the serum IGF system was correlated with steroid system but not with the litter size in pigs. Thus, this study implies that porcine litter size could be determined locally at the ovary level.
Saanen 종과의 누진교잡에 의한 재래산양 개량시험 제2보 , Saanen 종과 재래산양의 교잡종에 대한 비유능력의 변화
이길왕,최광수,탁태영,설동섭,이기만 ( K . W . Lee,K . S . Choi,T . Y . Tak,D . S . Sul,K . M . Lee ) 한국축산학회 1975 한국축산학회지 Vol.17 No.1
The data from 393 purebred and crossbred goats produced by matings of Korean Native Goats and Saanen, which were investigated at Livestock Experiment Station since 1963, were analyzed by least-square method to investigate changes of milking performance of the Korean Native Goats by grading up with the Saanen. This procedure adjusted the measurement data for surveyed year, parity, mating system and for the number of kid born at one birth. The results obtained are as follows. 1. The milk productions of the Korean Native Goats, the first filial, the second, and the third graded generations were 423.91, 90.97, 288.12, 354.81 and 372.76㎏, respectively. Their milk fat percentages were 4.15, 5.72, 5.37, 4.30 and 4.24, respectively. Their lactation periods were 239.7, 173.2, 214.3, 236.2 and 234.2 days, respectively. 2. The milk performances of the first filial, the second, the third graded generation and Saanen were significantly higher than those of Native Goats. The increment of milk character is the filial, the second, and the third graded generation, compared to the Native Goats were 197.15, 263.94 and 281.79㎏ in milk production; 41.14, 63.05 and 61.05 days in lactation period; -0.34, -1.42 and -1.48% in milk fat percentage. 3. The difference of milk production, lactation period and milk fat percentage between the third and the second graded generation was 17.89㎏, -2.00 days and -0.06, respectively. The difference between purebred Saanen and the third graded generation was 51.15㎏ for milk production, 1.55 days for lactation period, and -0.08% for milk fat percentage. However there was no significant difference between them. 4. The individual comparisons among the least square estimates showed that the difference between the 3 and 1 kid per birth was 130.4㎏ for milk production, 29. 4 days for lactation period, and -0.7% for milk fat percentage. The difference between the 3 .and 2 kid per birth was 3.13㎏, 11.6 days and 0.02%, respectively. The difference between the 2 and 1 kid per birth was 41.6㎏, 17.8 days and 0.09%, respectively.
Saanen 과의 누진교잡에 의한 재래산양 개량 시험 제1보 Saanen 과 재래산양의 교잡종에 대한 월령별 체중변화
이길왕,최광수,설동섭,이기만 ( K . W . Lee,K . S . Choi,D . S . Sul,K . M . Lee ) 한국축산학회 1973 한국축산학회지 Vol.15 No.4
The data from 112 purebred and crossbred goats produced by matings of Korean native goats and Saanen, which were investigated at Livestock Experiment Station since 1963, were analysed by least square method to investigate changes of body weights of the native goats by grading up with Saanen. This procedure adjusted the measurement data for environmental effects of year of birth, age of dam and litter size. The results obtained were as follows; 1. The body weight was measured and adjusted at birth, weaning, 12 and 18 months of age as 1.83㎏, 6.99㎏, 14.51㎏ and 18.90㎏ in Korean native goats, 2.98㎏. 12.25㎏, 25.49㎏ and 36.93㎏ in purebred Sannen, 2.69㎏, 11.14㎏, 23.03㎏ and 29.27㎏ in the first filial generation, and 2.78㎏, 11.85㎏, 24.77㎏ and 36.19㎏ in the second graded generation, respectively. 2. The body weights of first filial and second generation were heavier than those of native goats through all months of age. The increment of body weight in the first filial generation to the native goats was 0.86㎏ at birth, 4.15㎏ at weaning, 8.50㎏ at 12 months and 10.37㎏ at 18 months of age. In the second generation the increment of body weight was 0.94㎏, 4.85㎏, 10.26㎏ and 17.29㎏, respectively. 3. The differences of body weight between purebred Saanen and the first generation were 0.28㎏ at birth, 1.11㎏ at weaning 2.4㎏ at 12 months and 7.6㎏ at 18 months of age, but significant difference was found only at 18 months of age (p$lt;0.01). The body weight of the second generation was slightly lighter by 0.20 to 0.75㎏ than that of purebred Saanen through all months of age. 4. The second generation was heavier than the first generation by 0.08㎏ at birth. 0.71㎏ at weaning, 1.73㎏ at 12 months and 6.92㎏ at 18 months of age, but significant difference was found after 18 months of age (p$lt;0.05).