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Structure of Canine Gastrin Gene
강창원,강신애,조혜련 한국유전학회 1989 Genes & Genomics Vol.11 No.4
The full length canine (dog) gastrin gene was cloned from a genomic library by in situ plaque hybridization and mapped with several restriction endonucleases. Two clones containing 12.5 kb and 14.6 kb genomic DNA fragments were isolated among over 500,000 recombinant phages by using the canine gastrin cDNA as a hybridization probe. Restriction mapping of the clones reveals that they share the identical 11.0 kb fragment containing gastrin gene of 2.5 kb. Comparison of partial genomic sequence with cDNA sequence reveals three exons and two introns like human gastrin gene. Splicing junctions are identical to the human and follow the GU/AG consensus sequence rule of mRNA processing. The canine intron I, however, is only 1.7 kb long unlike the human 3.5 kb intron I. The 5’ flanking region of the gene contains the conserved sequence T-A-T-A-A, which is present at 26 bp upstream from the putative cap site. The sequence G-A-C-T-C-A-T-A-T, found at 86 bp upstream in the human and other eukaryotic genes, is not detected. The sequence T_5-A₂-T-A-T₄-A-C-T₂-A-T₃-A-T₃-A-T₃, similar to a human RNA polymerase II terminator sequence T_9-A₂-T_5-A-T₄-A-T₄-A-T₄, appears to be the canine terminator. However, it has a inverted repeat of 6.5 bp sequence instead of the human 10.5 bp repeat unit.
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강창원,강경래,남기택 한국가금학회 1995 한국가금학회지 Vol.22 No.2
Vitarnin A levels of egg yolk from hens fed diets supplemented with vitamin A increased slowly up to Week 4, while the values rapidly increased thereafter. The highest value(360% of initial value) was observed at Week 8 in birds fed 64,000 IU of vitamin A/kg diet. On the other hand, the contents of vitamin E in egg yolk rapidly increased in the first 2 weeks of the experiment and then stabilized up to Week 8. However, the efficiency of egg transfer decreased(P<0.05) as dietary vitamins A and E levels increased. It took at least 4 weeks for the increased levels of vitamin A and E in eggs from hens fed diets supplemented with each vitamin to recover the normal levels. Vitamin E levels of egg yolk in hens fed the vitamin A supplemented diets decreased as dietary vitamin A level increased (P<0.05). On the other hand, a slightly reduced levels of vitamin A levels of egg yolks from hens fed the vitamin E supplemented diet for 4 weeks of experiment was recovered back to control level at Week 8(P<0.05). Supplemental vitamin A markedly decreased the yolk deposition of vitamin F when two vitamins were supplemented simultaneously. The yolk deposition of vitamin A decreased as high level of vitamin B was supplemented, suggesting an antagonistic interactions between the two vitamins.
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