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Yorkshire 종 돼지에서 에스트로겐 수용체 유전자 좌위 변이와 산자수간의 관련성
서동삼,고용,박희복,양성호,김기동,박성수,홍기창 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.2
This study was conducted to investigate a possible association of the porcine estrogen receptor(ER) locus with the total number of born(TNB) and number of born alive(NBA) in Yorkshire pigs. Using DNAs extracted from 61 Yorkshire pigs, the ER genotype was determined by PCR-RFLP and Southern blot analysis. The ER allele frequencies of two types of A and B were 0.38 and 0.62, respectively. The least squares means of the litter size per ER genotypes were evaluated. The TNB and NBA were found to be associated with an specific ER allele. To estimate the quantitative effects of the different ER genotypes more accurately, a linear regression analysis was performed. The regression coefficients for ER genotypes were statistically significant, and the additive effect of ER locus was detected. The genotype at the porcine ER locus has an application potential for marker-assisted selection for litter size in pigs.
한국 재래 오골계의 초기산란능력과 혈중 Insulin - like Growth Factor - l 간의 연관성
서동삼,고용,이철영,홍기창,강우진,윤준수 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.6
There are considerable evidences that growth factors are involved in the regulation of ovulation rate and oviductal development in birds. This study was conducted to investigate the association of early egg productivity with serum insulin-like growth factor-I (IGF-I) in Korean Native Ogol Chicken (KNOC). Whole blood was collected from KNOC at the age of 20, 30, and 40 weeks. Serum IGF-I concentration was measured by radioimmunoassay. Chicks were divided into high and low groups, based on egg production and IGF-I concentration until 40 weeks of age. IGF-I was significantly higher at 30 and 40 weeks of age in high egg production group compared to that of low egg production one (P$lt;0.05). This result indicates that serum IGF-I and number of egg production have a positive association and implies the regulation of egg production by IGF-I in KNOC. Further studies will need to understand the endocrine mechanism of egg production to describe the endocrine regulators in KNOC.
혈중 내분비인자 함량과 Insulin - like Growth Factor-1 유전자 다형현상이 한국 재래 오골계의 초산일령에 미치는 영향
서동삼,고용,홍기창,강우진,윤준수 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.2
There are considerable evidences that endocrine factors are involved in the development and differentiation of ovary in the poultry. However, the effects of IGF-1 and steroid hormones on the sexual maturity of Korean Native Ogol Chicken (KNOC) have not yet been studied. Therefore, this study was performed to examine the expression of endocrine factors (IGF-I, estradiol, and progesterone) in KNOC and to find relationships between serum concentrations of these and the sexual maturity. Serum IGF-1, estradiol, and progesterone concentrations were measured by radioimmunoassays. Based on IGF-1 PCR-RFLP patterns. chickens were divided into three groups, that is. AA. AB, and BB genotypes. Compared with the AA and AB genotypes. the BB genotypes layed the first egg at a younger (P$lt;0.05) age. Moreover. progesterone concentration at 20wk of age before the sexual maturity was greater (P$lt;0.05) in the BB genotype than in the AA and AB genotypes. Although a significant difference in estradiol concentration between the three genotypes was not detected. the BB genotype tended to have a greater concentration of this hormone than the AA and AB genotypes. On the other hand, IGF-1 concentration was greater in the AA and AB genotypes than in the BB genotype. Concentrations of IGF-1. estradiol, and progesterone were negatively correlated with the age when the bird first layed an egg in the AA genotype. Taken together, the results suggest that the sexual maturity in KNOC is likely to result from complex interactions of several endocrine factors.
배반엽성세포를 이용한 카이메라 닭 ( 화이트레그혼 ↔ 오골계 ) 생산
서동삼(D . S . Seo),홍영호(Y . H . Hong),최강덕(K . D . Choi),양무희(M . H . Yang),신영수(Y . S . Shin),한재용(J . Y . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.1
The chicken embryo contains 30,000 to 60,000 cells at development stage X, the blastodermal cells at this stage are believed to he pluripotent and subsequently contribute to melanocytes derived from the neural crest, to erythrocytes and to germ cells. In this study, blastodermal cells were isolated from stage X embryos of Korean Native Ogol Chicken (KNOC). The isolated blastodemial cells were transfected with liposome and dispersed by pipetting. A 2㎕ volume of cell suspension was microinjected into the subgerminal cavity of recipient embryos at an equivalent stage of development of the donor and recipient. Of the 332 White Leghorn embryos that were injected with KNOC blastodermal cells, three unhitched embryos were phenotypically chimeric with respect to feather color and one embryo hatched. This technique may be useful in development of a transgenic chicken production system.
동물 번식에서의 Transforming Growth Factor-β 의 역할
정명호,서동삼,고용 한국축산학회 1999 한국축산학회지 Vol.41 No.3
Mammalian transforming growth factor-βs (TGF-βs) consist of three TGF-β isoforms (TGF-β1-β3) are glycosylated polypeptides and act via type I and II receptor complex which is a serine/threonine protein kinase. They show both overlapping and distinct spatial and temporal patterns of the expression during different reproductive stages in mammals. TGF-βs have several regulatory roles in cell proliferation and differentiation, cell migration, tissue remodeling, extracellular matrix modulation, bone formation, angiogenesis, steroidogenesis, wound healing, and immune regulation. Based on the multifunctional property of TGF-βs, they are suggested to play an important role in animal reproduction. The present article summarizes the current knowledge on the structure, signal transduction, and functions of TGF-β on animal reproduction and development.
임신된 마우스 자궁내의 Transforming Growth Factor - β mRNA 의 발현
정명호,서동삼,고용 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.5
Along with several hormones and growth factors, transforming growth factor-β(TGF- β) has been shown to be involved in animal reproductive processes. To further understand the potential functions of TGF-β in the uterus, studies on the expression of the three mammalian TGF-β isoforms (TGF-β1, -β2, and -β3) and receptors (type I and II ) in pregnant mouse uterus were carried out using reverse transcription polymerase chain reaction (RT-PCR). Temporal and distinct patterns of their expression were observed, showing that the mRNA of TGF-β1, β2, and -β3 were highly expressed during implantation and mid-pregnancy, during late pregnancy, and during postimplantation, respectively. In receptors, TβR I mRNA was highly expressed during preimplantation whereas TβR Ⅱ mRNA during postimplantation. Such temporal mRNA expressions of TGF-β isoforms and their receptors during pregnancy indicate that TGF-βs function temporally and imply that this distribution can be correlated with specific developmental processes.
한국 재래 오골계의 성장호르몬 유전자 다형현상과 혈중 Insulin - like Growth Factor-1 의 함량 및 산란능력간의 관련성
이재현,서동삼,고용,홍기창,강우진,윤준수 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.3
The growth hormone (GH) affects a wide range of biological processes such as proliferation and differentiation in ovary or target tissues by releasing IGF-I from the liver. Thus, IGF-I has been reported to have important roles on the formation of follicles, ovarian development and ovulation rates in chickens. So, in order to improve the egg productivity of the Korean Native Ogol Chicken (KNOC), this study was conducted for the identification of the polymorphism of GH gene and for its possible association with egg productivity and serum IGF-I concentration. The RFLP patterns (AA, BB, CC, DD, EE, and FF genotypes) for GH gene were identified by the Msp I restriction enzyme. The dominant genotypes were BB and EE, which were 32.9% and 41.4%, respectively. Highest number of egg produced and IGF-I concentration at 40 wks indirectly imply their association. And the statistical significances were observed in age at first egg laying, egg production number, and IGF-1 concentration except for egg weight and body weight (p$lt;0.05) by GH genotypes, indicating that egg productivity in KNOC is associated with GH genotypes. Taken together, these results suggested the possibility of the GH genotypes as a marker for the improvement of egg productivity and considered the requirement of research on other endocrine factors affecting ovulation in KNOC.